Identification of novel Salmonella virulence genes involved in invasion and intracellular survival:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
mbv
2009
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| Schriftenreihe: | biology
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 157 S. graph. Darst. 21 cm |
| ISBN: | 9783866646445 |
Internformat
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS ABBREVIATIONS VII ABSTRACT X ZUSAMMENFASSUNG XII 1
INTRODUCTION 1 1.1 SALMONELLA AND SALMONELLOSIS 1 1.2 SALMONELLA HOST
CELL INVASION 2 1.3 INTRACELLULAR GROWTH OF SALMONELLA 3 1.4 VIRULENCE
DETERMINANTS OF SALMONELLA 5 1.4.1 SALMONELLA PATHOGENICITY ISLANDS 6
1.4.2 PLASMID-ENCODED VIRULENCE GENES 11 1.4.3 OTHER VIRULENCE GENES 11
1.5 REGULATION OF VIRULENCE GENES 13 1.5.1 ENVIRONMENTAL REGULATION 14
1.5.2 GENETIC REGULATION 14 1.6 FLAGELLAR REGULON OF SALMONELLA 22 1.7
LYSR FAMILY OF TRANSCRIPTIONAL REGULATORS 24 1.7.1 ROLE OF LRHA
HOMOLOGUES IN BACTERIAL VIRULENCE 25 1.8 METHODS AND TOOLS FOR
IDENTIFICATION AND CHARACTERISATION OF SALMONELLA VIRULENCE GENES 26
1.8.1 METHODS FOR IDENTIFICATION OF NEW VIRULENCE GENES 26 1.8.2
REPORTER SYSTEMS 29 1.8.3 MODEL SYSTEMS FOR THE STUDY OF SALMONELLA 30 2
RATIONALE AND AIMS OF RESEARCH 33 3 RESULTS 35 3.1 IDENTIFICATION AND
CHARACTERISATION OF NOVEL SALMONELLA GENES 35 3.1.1 ROLE OF NOVEL
SALMONELLA GENES IN HOST CELL INVASION IN VITRO 35 3.1. BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/998075302 DIGITALISIERT DURCH 3.2.4 LRHA
IS A NEGATIVE REGULATOR OF MOTILITY IN SALMONELLA TYPHIMURIUM 48 3.2.5
LRHA EXPRESSION IS POSITIVELY AUTOREGULATED 48 3.2.6 LRHA REGULATES THE
EXPRESSION OF TWO COMPONENT SYSTEM ACTIVATORS OF HILA TRANSCRIPTION. 49
3.2.7 LRHA REGULATES VIRULENCE GENES ENCODED WITHIN MULTIPLE
PATHOGENICITY ISLANDS AND THROUGHOUT THE SALMONELLA GENOME. 51 3.2.8
LOSS OF HILA EXPRESSION IS INDEPENDENT OF HHA, STPA, CSRB/C AND RFRA/B
REGULATORS. 54 3.2.9 LRHA DIRECTLY INTERACTS WITH THE PROMOTER REGIONS
OF ITS TARGET GENES. 57 3.2.10 PRODUCTION OF LRHA IS GROWTH PHASE
DEPENDENT 60 3.2.11 IN VIVO CHARACTERISATION OF IRHA 61 4 DICUSSION 62
4.1 CONTRIBUTION OF NEW VIRULENCE GENES TO SALMONELLA VIRULENCE 62 4.2
LRHA AS A GLOBAL REGULATOR OF SALMONELLA VIRULENCE GENE EXPRESSION 64
4.3 OUTLOOK 74 5 METHODS 75 5.1 MOLECULAR BIOLOGY METHODS 75 5.1.1 DNA
ISOLATION 75 5.1.2 ISOLATION AND CONCENTRATION OF DNA FROM AQUEOUS
SOLUTIONS 76 5.1.3 RNA PREPARATION 76 5.1.4 DETERMINATION OF RNA QUALITY
77 5.1.5 ELECTROPHORESIS AND DETECTION OF NUCLEIC ACIDS ON AGAROSE GELS
77 5.1.6 DETERMINATION OF CONCENTRATION OF NUCLEIC ACIDS 77 5.1.7
MICROARRAY ANALYSIS OF GENE EXPRESSION 77 5.1.8 CONSTRUCTION OF DELETION
MUTANTS 80 5.1. 5.2.3 ELECTROPORATION OF BACTERIA 88 5.2.4 MOTILITY
ASSAY 89 5.2.5 COLONY PCR 90 5.2.6 BACTERIAL STRAINS AND LONG TERM
STORAGE 90 5.2.7 MEDIA AND GROWTH CONDITIONS 91 5.2.8 ANIMAL EXPERIMENTS
91 5.3 CELL CULTURE METHODS 92 5.3.1 MAINTENANCE OF CELL LINES 92 5.3.2
PREPARATION OF STOCKS 92 5.3.3 INVASION ASSAY 92 6 MATERIALS 93 6.1
BACTERIAL STRAINS AND VECTORS 93 6.2 PRIMERS 97 6.3 LABORATORY
EQUIPMENTS 101 6.4 CONSUMABLES 101 6.5 REAGENTS 102 6.6 COMMERCIAL KITS
103 6.7 ENZYMES 103 6.8 SOLUTIONS, MEDIUM AND BUFFERS 103 6.8.1 AGAROSE
GEL ELECTROPHORESIS BUFFERS 103 6.8.2 BACTERIAL CULTURE MEDIUM 103 6.8.3
ANTIBIOTICS 104 6.8.4 PROTEIN STUDIES 104 6.8.5 BETA-GALACTOSIDASE ASSAY
106 6.8.6 PLASMID PREPARATION BUFFERS 106 6.8.7 RNA PREPARATION 106
6.8.8 MICROARRAY STUDIES 107 6.9 SOFTWARES 107 7 REFERENCES 107 8
APPENDIX 143 9 ACKNOWLEDGEMENTS 153 10 PUBLICATIONS 154 11 CURRICULUM
VITAE 155
|
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| author | Chikkaballi Anne Gowda, Deepak 1978- |
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| author_facet | Chikkaballi Anne Gowda, Deepak 1978- |
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| dewey-full | 571.9934411 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.9934411 |
| dewey-search | 571.9934411 |
| dewey-sort | 3571.9934411 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-09T22:09:12Z |
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| isbn | 9783866646445 |
| language | English |
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| physical | XIV, 157 S. graph. Darst. 21 cm |
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| spelling | Chikkaballi Anne Gowda, Deepak 1978- Verfasser (DE-588)139800816 aut Identification of novel Salmonella virulence genes involved in invasion and intracellular survival von Deepak Chikkaballi Anne Gowda Berlin mbv 2009 XIV, 157 S. graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier biology Zugl.: Berlin, Humboldt-Univ., Diss., 2008 Gen (DE-588)4128987-0 gnd rswk-swf Virulenzfaktor (DE-588)4354782-5 gnd rswk-swf Salmonella typhimurium (DE-588)4121604-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Salmonella typhimurium (DE-588)4121604-0 s Virulenzfaktor (DE-588)4354782-5 s Gen (DE-588)4128987-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018885733&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chikkaballi Anne Gowda, Deepak 1978- Identification of novel Salmonella virulence genes involved in invasion and intracellular survival Gen (DE-588)4128987-0 gnd Virulenzfaktor (DE-588)4354782-5 gnd Salmonella typhimurium (DE-588)4121604-0 gnd |
| subject_GND | (DE-588)4128987-0 (DE-588)4354782-5 (DE-588)4121604-0 (DE-588)4113937-9 |
| title | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival |
| title_auth | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival |
| title_exact_search | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival |
| title_full | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival von Deepak Chikkaballi Anne Gowda |
| title_fullStr | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival von Deepak Chikkaballi Anne Gowda |
| title_full_unstemmed | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival von Deepak Chikkaballi Anne Gowda |
| title_short | Identification of novel Salmonella virulence genes involved in invasion and intracellular survival |
| title_sort | identification of novel salmonella virulence genes involved in invasion and intracellular survival |
| topic | Gen (DE-588)4128987-0 gnd Virulenzfaktor (DE-588)4354782-5 gnd Salmonella typhimurium (DE-588)4121604-0 gnd |
| topic_facet | Gen Virulenzfaktor Salmonella typhimurium Hochschulschrift |
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