Cilia: [1] Structure and motility
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
91 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 332 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123749734 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804141009109516288 |
|---|---|
| adam_text | Titel: Bd. 1. Cilia. Structure and motility
Autor:
Jahr: 2009
CONTENTS
Contributors
Preface
1. Cryo-Electron Microscope Tomography to Study Axonemal
Organization
Daniela Nicastro
I. Introduction and Rationale 2
II. Methods and Materials 12
III. Discussion 30
References 32
2. Electron Microscopic Imaging and Analysis of Isolated
Dynein Particles
Anthony J. Roberts and Stan A. Burgess
I. Introduction 42
II. Rationale 45
III. Methods 46
VI. Summary 59
References 60
3. Immunogold Labeling of Flagellar Components In Situ
Stefan Geimer
I. Introduction 64
II. Methods for Immunogold Labeling of Flagellar Components 67
III. Materials 74
IV. Summary and Discussion 77
References 78
Scanning Electron Microscopy to Examine Cells and Organs
Jovcnal T. SanAgustin, John A. FoUit, Gregory Hendricks, and Gregory]. Pjzour
I. Introduction 82
II. Materials 82
III. Metal-Impregnation Method for Scanning EM Analysis of Mouse Kidneys
(modified from McManus et ah, 1993) 84
Contents
IV. Method for Scanning EM Visualization of Mouse Nodal Cilia 86
V. Summary 87
References 87
5. X-ray Fiber Diffraction Studies on Flagellar Axonemes
Kazuhiro Oitva, Shinji Kamimura, and Hiroyuki Iwamoto
I. Introduction 90
II. Rationale 91
HI. Methods 97
IV. Results and Discussion 105
V. Summary 107
References 108
6. Markers for Neuronal Cilia
Jacqueline S. Domire and Kirk Mykytyn
I. Introduction 112
II. Immunoenzymatic Labeling of Neuronal Cilia in Brain Sections 113
HI. Immunofluorescent Labeling of Neuronal Cilia in Brain Sections 115
IV. Visualization of Neuronal Cilia In Vitro 117
V. Summary 120
References 121
Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells
Heymut Omran and Niki T. Loges
I. Introduction 124
II. Antibodies 126
III. Materials and Methods 129
IV. Methods 129
V. Discussion 131
References 133
Irnmunoprecipitation to Examine Protein Complexes
Gregory]. Pazour
I. Introduction 135
II. General Procedure 137
HI. Controls for Irnmunoprecipitation Assays 139
IV. Summary 141
References 142
Contents
9. Tandem Affinity Purification of Ciliopathy-Associated Protein
Complexes
Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,
Marius Uejjing, and Ronald Roepman
I. Introduction 144
II. Methods 150
III. Data Evaluation 155
IV. Oudook 157
References 158
10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products
Miho Sakato
I. Chemical Crosslinking 162
II. Identification of Interaction Partners 164
III. Summary 170
References 170
11. Analysis of the Ciliary/Flagellar Beating of Chlamydonionas
Kenneth W. Foster
I. Introduction 174
II. Rationale 182
III. Techniques 182
IV. Future Directions 231
References 232
12. Assays of Cell and Axonemal Motility in Chlamydonionas reinhardtii
Ritsu Kamiya
I. Introduction 242
II. Motility Assessments in Live Cldamydomonas Cells 242
III. In Vitro Reactivation of Axonemal Beating 246
IV. Microtubule Sliding by Axonemal Dynein 248
References 251
13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain
Karl-Ferdinand Lcchtreck, Michael]. Sanderson, and George B. Witmam
I. Introduction 256
II. Materials and Equipment 257
HI. Methods 259
IV. Discussion 262
V. Summary 262
References 263
14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow
Yasushi Okada and Nobutaka Hirokawa
I. Introduction and Historical Background 266
II. Observation of Nodal Cilia Motility in Mouse Embryo 269
III. Observation of Nodal Flow in Mouse Embryo 276
IV. Observation of Nodal Cilia Motility and Nodal Flow in Rabbit Embryo 278
V. Discussion 281
References 284
15. Modification of Mouse Nodal Flow by Applying Artificial Flow
Shigenori Nonaka
I. Introduction 288
II. Solutions 289
III. Experimental Setup 289
IV. Methods 291
V. Discussion 295
References 296
16. Measuring Cilium-Induced Ca + Increases in Cultured Renal Epithelia
Helle A. Praetorius
I. Introduction 300
II. Methods 300
HI. Materials 310
IV. Summary/Conclusion 311
References 311
Index 315
Volume in Series 325
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| indexdate | 2024-07-09T22:09:03Z |
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| isbn | 9780123749734 |
| language | English |
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| series | Methods in cell biology |
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| spelling | Cilia [1] Structure and motility ed. by Stephen M. King ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2009 XII, 332 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 91 Methods in cell biology ... Molekularer Motor (DE-588)4720488-6 gnd rswk-swf Zilie (DE-588)4190826-0 gnd rswk-swf Dynein (DE-588)4236074-2 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Strukturanalyse (DE-588)4183787-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zilie (DE-588)4190826-0 s Dynein (DE-588)4236074-2 s Strukturanalyse (DE-588)4183787-3 s DE-604 Molekularer Motor (DE-588)4720488-6 s Genregulation (DE-588)4122166-7 s King, Stephen M. Sonstige (DE-588)141104457 oth (DE-604)BV035961549 1 Methods in cell biology 91 (DE-604)BV002534878 91 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018879749&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cilia Methods in cell biology Molekularer Motor (DE-588)4720488-6 gnd Zilie (DE-588)4190826-0 gnd Dynein (DE-588)4236074-2 gnd Genregulation (DE-588)4122166-7 gnd Strukturanalyse (DE-588)4183787-3 gnd |
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| title | Cilia |
| title_auth | Cilia |
| title_exact_search | Cilia |
| title_full | Cilia [1] Structure and motility ed. by Stephen M. King ... |
| title_fullStr | Cilia [1] Structure and motility ed. by Stephen M. King ... |
| title_full_unstemmed | Cilia [1] Structure and motility ed. by Stephen M. King ... |
| title_short | Cilia |
| title_sort | cilia structure and motility |
| topic | Molekularer Motor (DE-588)4720488-6 gnd Zilie (DE-588)4190826-0 gnd Dynein (DE-588)4236074-2 gnd Genregulation (DE-588)4122166-7 gnd Strukturanalyse (DE-588)4183787-3 gnd |
| topic_facet | Molekularer Motor Zilie Dynein Genregulation Strukturanalyse Aufsatzsammlung |
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