Histology protocols:
Gespeichert in:
Weitere Verfasser: | |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York, NY
Humana Press
2010
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Schriftenreihe: | Methods in molecular biology
611 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | X, 229 S. Ill., graph. Darst. 26 cm |
ISBN: | 9781603273442 1603273441 |
Internformat
MARC
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490 | 0 | |a Springer protocols | |
650 | 4 | |a Histocytological Preparation Techniques | |
650 | 4 | |a Histology |v Laboratory manuals | |
650 | 4 | |a Microscopy |x methods | |
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Datensatz im Suchindex
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adam_text | Titel: Histology protocols
Autor: Hewitson, Tim D.
Jahr: 2010
Contents
Preface............................................................ p
Contributors........................................................ ix
Part I: Tissue Processing
1. Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen
Retrieval for Light Microscopy........................................ 3
Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker
2. An Optimized RNA Extraction Method from Archival Formalin-Fixed
Paraffin-Embedded Tissue........................................... 19
Joon-Tong Chung and Stephen M. Hewitt
3. Laser-Capture Microdissection and Pressure Catapulting for the Analysis
of Gene Expression in the Renal Glomerulus............................. 29
Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly
Part II: Staining Techniques
4. Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix
in Tissue and Cultured Cells.......................................... 43
Josiane Smith-Clerc and Boris Hinz
5. Double Immunohistochemistry with Horseradish Peroxidase and Alkaline
Phosphatase Detection Systems....................................... 59
Vincent Sarrazy and Alexis Desmouliere
6. Retrogradely Transported Neuronal Tracers Combined with
Immunohistochemistry Using Free-Floating Brain Sections.................. 73
Emilio Badoer
7. High-Pressure Freezing, Chemical Fixation and Freeze-Substitution
for Immuno-electron Microscopy...................................... 87
Christian Muhlfeld
8. Lectin Histochemistry for Light and Electron Microscopy................... 103
Su Ee Wong, Catherine E. Winbanks, Chrishan S. Samuel,
and Tim D. Hewitson
9. Duplex In Situ Hybridization in the Study of Gene Co-regulation
in the Vertebrate Brain.............................................. 115
Raphael Pinaud and Jin K. Jeong
10. Special Stains for Extracellular Matrix................................... 131
Andrea Monte-Alto-Costa and Luis Cristovdo Porto
11. Active Staining of Mouse Embryos for Magnetic Resonance Microscopy........ 141
Alexandra Petiet and G. Allan Johnson
12. Immunohistochemical Detection of Tumour Hypoxia...................... 151
Richard J. Young and Andreas Mbller
VII
viii Contents
13. In Situ Localization of Apoptosis Using TUNEL.......................... 161
Tim D. Hewitson and Ian A. Darby
Part III: Imaging Techniques
14. Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons
in the Spinal Cord.................................................. 173
Martin Stebbing, Simon Potocnik, Pinglu Te, and Emilio Badoer
15. High-Resolution Confocal Imaging in Tissue............................. 183
Verena C. Wimmer and Andreas Moller
16. Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic
Range in Digital Photomicrography.................................... 193
J org Piper
17. Image Analysis and Quantitative Morphology............................ 211
Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos,
and Marcia Barbosa Aguila
Subject Index.......................................................... 227
|
any_adam_object | 1 |
author2 | Hewitson, Tim D. |
author2_role | edt |
author2_variant | t d h td tdh |
author_facet | Hewitson, Tim D. |
building | Verbundindex |
bvnumber | BV035977374 |
callnumber-first | Q - Science |
callnumber-label | QM556 |
callnumber-raw | QM556 |
callnumber-search | QM556 |
callnumber-sort | QM 3556 |
callnumber-subject | QM - Human Anatomy |
ctrlnum | (OCoLC)455818822 (DE-599)BSZ312381255 |
dewey-full | 611.0180721 |
dewey-hundreds | 600 - Technology (Applied sciences) |
dewey-ones | 611 - Human anatomy, cytology, histology |
dewey-raw | 611.0180721 |
dewey-search | 611.0180721 |
dewey-sort | 3611.0180721 |
dewey-tens | 610 - Medicine and health |
discipline | Medizin |
format | Book |
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id | DE-604.BV035977374 |
illustrated | Illustrated |
indexdate | 2024-07-09T22:08:53Z |
institution | BVB |
isbn | 9781603273442 1603273441 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018871331 |
oclc_num | 455818822 |
open_access_boolean | |
owner | DE-12 |
owner_facet | DE-12 |
physical | X, 229 S. Ill., graph. Darst. 26 cm |
publishDate | 2010 |
publishDateSearch | 2010 |
publishDateSort | 2010 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Histology protocols ed. by Tim D. Hewitson ... New York, NY Humana Press 2010 X, 229 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 611 Springer protocols Histocytological Preparation Techniques Histology Laboratory manuals Microscopy methods Histology / Laboratory manuals Hewitson, Tim D. edt Methods in molecular biology 611 (DE-604)BV035362695 611 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018871331&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Histology protocols Methods in molecular biology Histocytological Preparation Techniques Histology Laboratory manuals Microscopy methods |
title | Histology protocols |
title_auth | Histology protocols |
title_exact_search | Histology protocols |
title_full | Histology protocols ed. by Tim D. Hewitson ... |
title_fullStr | Histology protocols ed. by Tim D. Hewitson ... |
title_full_unstemmed | Histology protocols ed. by Tim D. Hewitson ... |
title_short | Histology protocols |
title_sort | histology protocols |
topic | Histocytological Preparation Techniques Histology Laboratory manuals Microscopy methods |
topic_facet | Histocytological Preparation Techniques Histology Laboratory manuals Microscopy methods |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018871331&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT hewitsontimd histologyprotocols |