Sugars in early and late polyketide biosynthesis: functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Verl. Dr. Hut
2009
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Pharmazeutische Biologie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 131 S. Ill., graph. Darst. 210 mm x 148 mm, 197 gr. |
| ISBN: | 9783868532838 |
Internformat
MARC
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|---|---|---|---|
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| 100 | 1 | |a Engels, Silke |d 1975- |e Verfasser |0 (DE-588)140300287 |4 aut | |
| 245 | 1 | 0 | |a Sugars in early and late polyketide biosynthesis |b functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 |c Silke Engels |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a München |b Verl. Dr. Hut |c 2009 | |
| 300 | |a VI, 131 S. |b Ill., graph. Darst. |c 210 mm x 148 mm, 197 gr. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Pharmazeutische Biologie | |
| 502 | |a Zugl.: Bonn, Univ., Diss., 2009 | ||
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| 650 | 0 | 7 | |a Streptomyces |0 (DE-588)4183668-6 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
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|---|---|
| adam_text | TABLE OF CONTENTS LIST OF ABBREVIATIONS V 1 SUMMARY 1 2 INTRODUCTION 3
2.1 STREPTOMYCETES AS POLYKETIDE FACTORIES 3 2.2 BIOSYNTHESIS OF
POLYKETIDES 7 2.2.1 POLYKETIDE SYNTHASES 7 2.2.2 POST-PKS TAILORING 11
2.2.3 ENGINEERED BIOSYNTHESIS OF UNNATURAL NATURAL PRODUCTS 12 2.3
SUGAR MOIETIES IN EARLY AND LATE POLYKETIDE BIOSYNTHESIS 13 2.3.1
RIFAMYCINS 13 2.3.2 FUNCTIONAL STUDIES OF RISS, RIJK AND RIFL/L IN EARLY
RIFAMYCIN BIOSYNTHESIS 14 2.3.3 PENTANGULAR AROMATIC POLYKETIDES 16
2.3.4 IB-00208 AND POST-PKS TAILORING IN LATE POLYKETIDE BIOSYNTHESIS 16
3 GOALS OF THE PRESENT STUDY .*. 19 4 RESULTS AND
DISCUSSION..........»*....*».....»....**».....**......*»»»*»»»***»»»»*»»**»»»**»»»»»
22 4.1 INVESTIGATING THE INITIAL STEPS IN RIFAMYCIN BIOSYNTHESIS 22
4.1.1 THE RIFL-OXIDOREDUCTASE ASSAY 23 4.1.2 IMPACT OF DIFFERENT
NITROGEN SOURCES ON THE RIFL ASSAY 27 4.1.3 APPLICATION OF DIFFERENT
HYDRIDE ACCEPTORS IN THE RIFL ASSAY 29 4.1.4 PURIFICATION OF CELL-FREE
EXTRACTS FROM STREPTOMYCES LIVIDANS PHGF7604 30 4.1.5 SIZE DETERMINATION
OF PROTEINS FROM STREPTOMYCES LIVIDANS PHGF7604 32 4.1.6 DETECTION OF
KANOSAMINE 33 4.1.7 H-NMR EXPERIMENTS OF RIFL-INCUBATION SAMPLES 35
4.1.8 RT-PCR OF CDNA FROM STREPTOMYCES LIVIDANS PHGF7604 37 4.1.
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/999629123 DIGITALISIERT
DURCH TABLE OF CONTENTS 4.2.7 HETEROLOGOUS EXPRESSION OF THE POSITIVE
COSMID SEQUENCES 55 4.2.8 SUBCLONING AND SEQUENCING OF SELECTED COSMID
CLONES 59 5 MATERIALS AND METHODS (EXPERIMENTAL PROCEDURES) 73 5.1
CHEMICALS, LABORATORY EQUIPMENT AND OTHER MATERIALS 73 5.1.1 CHEMICALS
73 5.1.2 MOLECULAR BIOLOGICAL KITS, ENZYMES AND MOLECULAR WEIGHT MARKERS
75 5.1.3 LABORATORY EQUIPMENT AND OTHER MATERIALS 76 5.1.4 STERILISATION
OF SOLUTIONS AND EQUIPMENT 77 5.1.5 PREPARATION OF MILLIPORE WATER 77
5.1.6 CLEAN ROOM CONDITIONS 77 5.2 BIOLOGICAL MATERIAL, PLASMIDS AND
OLIGONUCLEOTIDES 77 5.3 BUFFERS AND SOLUTIONS 80 5.4 MICROBIOLOGICAL
METHODS 83 5.4.1 CULTURE MEDIA AND ANTIBIOTICS 83 5.4.2 CULTIVATION AND
LONG TERM STORAGE OI STREPTOMYCES LIVIDANS STRAINS 84 5.4.3 CULTIVATION
OF STREPTOMYCES SP. GW2/5831 85 5.4.4 PREPARATION OF A SPORE SUSPENSION
FROM STREPTOMYCES ALBUS FOR CONJUGATION 86 5.4.5 CULTIVATION AND LONG
TERM STORAGE OF RECOMBINANT STREPTOMYCES ALBUS STRAINS... 86 5.4.6
CULTIVATION AND LONG TERM STORAGE OF RECOMBINANT E. COLI STRAINS 87
5.4.7 GROWTH KINETICS OF STREPTOMYCES LIVIDANS 87 5.5 MOLECULAR
BIOLOGICAL METHODS 87 5.5.1 ISOLATION OF GENOMIC DNA FROM GRA TABLE OF
CONTENTS 5.6.3 REMOVAL OF LOW MOLECULAR WEIGHT COMPONENTS FROM CRUDE
CELL-FREE EXTRACTS.. 108 5.6.4 CONCENTRATION OF PRE-PURIFIED PROTEINS
FROM COLUMN ELUENTS 108 5.6.5 COLORIMETRIC QUANTITATIVE ANALYSIS BASED
ON BRADFORD S METHOD 109 5.6.6 UV-SPECTROSCOPY 109 5.6.7 PROTEIN
SEPARATION VIA POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE) 110 5.7
ANALYTICAL METHODS 112 5.7.1 THIN LAYER CHROMATOGRAPHY (TLC) 112 5.7.2
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 112 5.7.3 FAST PROTEIN
LIQUID CHROMATOGRAPHY (FPLC) 112 5.7.4 MASS SPECTROSCOPY 113 5.7.5
H-NMR EXPERIMENTS 114 5.8 SYNTHESIS OF 2-OXOGLUTARAMATE 114 6 APPENDIX
116 7 CURRICULUM VITAE 118 8 SELBSTAENDIGKEITSERKLAERUNG 120 9 REFERENCES
121 III
|
| any_adam_object | 1 |
| author | Engels, Silke 1975- |
| author_GND | (DE-588)140300287 |
| author_facet | Engels, Silke 1975- |
| author_role | aut |
| author_sort | Engels, Silke 1975- |
| author_variant | s e se |
| building | Verbundindex |
| bvnumber | BV035967763 |
| ctrlnum | (OCoLC)612434509 (DE-599)DNB999629123 |
| dewey-full | 615.1901 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 615 - Pharmacology and therapeutics |
| dewey-raw | 615.1901 |
| dewey-search | 615.1901 |
| dewey-sort | 3615.1901 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV035967763 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:08:44Z |
| institution | BVB |
| isbn | 9783868532838 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018861834 |
| oclc_num | 612434509 |
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| owner_facet | DE-12 DE-19 DE-BY-UBM |
| physical | VI, 131 S. Ill., graph. Darst. 210 mm x 148 mm, 197 gr. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Verl. Dr. Hut |
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| series2 | Pharmazeutische Biologie |
| spelling | Engels, Silke 1975- Verfasser (DE-588)140300287 aut Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 Silke Engels 1. Aufl. München Verl. Dr. Hut 2009 VI, 131 S. Ill., graph. Darst. 210 mm x 148 mm, 197 gr. txt rdacontent n rdamedia nc rdacarrier Pharmazeutische Biologie Zugl.: Bonn, Univ., Diss., 2009 Xanthon (DE-588)4332380-7 gnd rswk-swf Biosynthese (DE-588)4006902-3 gnd rswk-swf Gencluster (DE-588)4355303-5 gnd rswk-swf Rifamycin (DE-588)4178141-7 gnd rswk-swf Streptomyces (DE-588)4183668-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rifamycin (DE-588)4178141-7 s Biosynthese (DE-588)4006902-3 s Streptomyces (DE-588)4183668-6 s Xanthon (DE-588)4332380-7 s Gencluster (DE-588)4355303-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018861834&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Engels, Silke 1975- Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 Xanthon (DE-588)4332380-7 gnd Biosynthese (DE-588)4006902-3 gnd Gencluster (DE-588)4355303-5 gnd Rifamycin (DE-588)4178141-7 gnd Streptomyces (DE-588)4183668-6 gnd |
| subject_GND | (DE-588)4332380-7 (DE-588)4006902-3 (DE-588)4355303-5 (DE-588)4178141-7 (DE-588)4183668-6 (DE-588)4113937-9 |
| title | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 |
| title_auth | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 |
| title_exact_search | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 |
| title_full | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 Silke Engels |
| title_fullStr | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 Silke Engels |
| title_full_unstemmed | Sugars in early and late polyketide biosynthesis functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 Silke Engels |
| title_short | Sugars in early and late polyketide biosynthesis |
| title_sort | sugars in early and late polyketide biosynthesis functional studies of rifl rifk and rifm in rifamycin biosynthesis towards the characterisation of a pks gene cluster from streptomyces sp gw2 5831 encoding the biosynthesis of the polycyclic xanthone ib 00208 |
| title_sub | functional studies of rifL, rifK and rifM in rifamycin biosynthesis ; towards the characterisation of a PKS gene cluster from Streptomyces sp. GW2/5831, encoding the biosynthesis of the polycyclic xanthone IB-00208 |
| topic | Xanthon (DE-588)4332380-7 gnd Biosynthese (DE-588)4006902-3 gnd Gencluster (DE-588)4355303-5 gnd Rifamycin (DE-588)4178141-7 gnd Streptomyces (DE-588)4183668-6 gnd |
| topic_facet | Xanthon Biosynthese Gencluster Rifamycin Streptomyces Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018861834&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT engelssilke sugarsinearlyandlatepolyketidebiosynthesisfunctionalstudiesofriflrifkandrifminrifamycinbiosynthesistowardsthecharacterisationofapksgeneclusterfromstreptomycesspgw25831encodingthebiosynthesisofthepolycyclicxanthoneib00208 |