Cilia: [2] Motors and regulation
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
92 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 230 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123749741 |
Internformat
MARC
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| 245 | 1 | 0 | |a Cilia |n [2] |p Motors and regulation |c ed. by Stephen M. King ... |
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| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2009 | |
| 300 | |a XIV, 230 S., [4] Bl. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804140980013629440 |
|---|---|
| adam_text | CONTENTS
Contributors
Preface
1.
Bioinformatic Approaches to Dynein Heavy Chain Classification
Toshiki Yagi
I. Introduction
2
II. Methods
3
III. Materials
5
IV. Results and Discussion
6
References
g
2.
Identification and Characterization of Dynein Genes in Tetrahymena
David E. Wilkes, Nicole Bennardo, Clarence W. C, Chan, Yu-Loung Chang,
Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime,
Julianna
Erickson,
Jonathan Hetzel, Erin E.
Heyer,
Mark
J. Hubenschmidt,
Ekaterina Kniazeva,
Hallie
Kuhn,
Michelle hum, Andrea Sand, Alicia
Schep, Oksana Sergeeva,
Natt
Supab, Caroline
R.
Townsena, LJesl Van Ryswyk, Hadley E.
Watson,
Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J.
Asai
I. Introduction
12
II. Methods and Results
14
III. Discussion
26
References
27
3.
Purification of Axonemal Dyneins and Dynein-Associated Components
from Chkmydomonas
Stephen M. King
I. Introduction
32
II. Detachment and Isolation of
Flagella
34
III.
Flagellar Demembranation
35
IV. Dynein Extraction from
Flagellar
Axonemes
36
V. Fractionation of
Flagellar
Extracts
36
VI. Immunoprecipitation from Ceil Body Extracts
44
VII.
Conclusions
46
References
46
Contents
4.
Purification
of Dyneins from Sperm
Flagella
Kazuo Inaba and Katsutoshi Mizuno
I. Introduction
50
II. Short Protocol for Isolation of Sperm Outer Arm Dynein
from the Ascidian C.
intestinais
51
III. Collection of Sperm
52
IV. Isolation of Sperm
Flagella
54
V. Purification of Outer Arm Dyneins
55
VI. Regulation of Outer Arm Dynein
61
VII.
Purification of Inner Arm Dyneins
61
VIII.
Conclusion
62
References
62
5.
Protein Engineering Approaches to Study the Dynein Mechanism
using a Dictyosteliunt Expression System
Takahide
Kon,
Tomohiro Shima, and Kazuo Sutoh
I. Introduction
66
II. Preparation of
Recombinant
Dynein
67
HI. FRET-Based Detection of Dynein s Conformational Changes
72
IV. Motility Assays
75
References
80
6.
Biophysical Measurements on Axonemal Dyneins
Hiroaki
Kojima,
Shiori
Toba,
Hitoshi Sakakibara, and Kazuhiro Oiwa
I. Introduction
84
II. Materials and Methods
85
III. Results and Discussion
100
IV. Summary
102
References
103
7.
Protein Electroporation into Chlamydomonas for Mutant Rescue
Masahito Hayashi and Ritsu Kamiya
I. Introduction
107
II. Materials and Methods
108
III. Discussion
110
References
110
Contents
8.
Analysis of the Role of Nucleotides in Axonemal Dynein Function
Chikako Shingyoji
I. Introduction
114
II. Materials and Methods
117
III. Methods
119
IV. Results and Discussion
122
V. Outlook
129
References
130
9.
The Regulation of Dynein-Driven Microtubule Sliding in Chlatnydomonas
Flagella
by Axonemal Kinases and Phosphatases
Candice
A. Ehm,
Winfieli S. Sale, and Maureen Wirschell
I. Introduction
134
II. The Chlamydomonas Experimental System: Axoneme Isolation
and the
Flagellar
Mutants
137
III. In Vitro Microtubule Sliding Assays: The Method and Discovery
of Kinases and Phosphatases that Regulate Dynein
138
IV. Biochemical Analysis of Kinases and Phosphatases Localized
to the Chlamydomonas Axoneme
142
V. Discussion of the Regulatory Model and Concluding Remarks
145
References
147
10.
Analysis of
Redox-Sensitive
Dynein Components
Ken-idii Wakabayashi
I. Introduction
154
II. Materials and Reagents
155
III. Methods
155
References
160
11.
Calcium Regulation of Ciliary Motility: Analysis of Axonemal
Calcium-Binding Proteins
Christen DiPetrillo and Elizabeth Smith
I. Introduction
164
II. How Does Calcium Enter Motile Cilia?
164
III. What Ciliary Proteins Serve as Calcium Sensors?
165
IV. How Does a Change in Calcium Concentration Modulate Motility?
173
V. Summary
176
References
176
Contents
12. Isolation
and Analysis of
Radial
Spoke
Proteins
Pooja Kdekar,
Mei Wei,
ana Pinjen
Yang
I. Introduction
182
II. Isolation of Intact RS Complexes
183
III. Two-Dimensional Gel Electrophoresis of the RS Complexes
188
IV. Blue Native PAGE for Characterizing Subparticles in RS Complexes
192
V. Summary
195
References
195
13.
Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii
Oavid R. Mitchell and Brandon Smith
I. Introduction
198
II. Basic Methods for Central Pair Structure Analysis by Thin Section
Electron Microscopy
199
III. Genetic Dissection of the Central Pair
201
IV. Biochemical Dissection of the Central Pair
205
V. Methods to Study Central Pair Structural Conformation
207
VI. Summary
211
References
212
Index
215
Volume in Series
223
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| indexdate | 2024-07-09T22:08:35Z |
| institution | BVB |
| isbn | 9780123749741 |
| language | English |
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| physical | XIV, 230 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
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| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Cilia [2] Motors and regulation ed. by Stephen M. King ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2009 XIV, 230 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 92 Methods in cell biology ... Genregulation (DE-588)4122166-7 gnd rswk-swf Strukturanalyse (DE-588)4183787-3 gnd rswk-swf Dynein (DE-588)4236074-2 gnd rswk-swf Zilie (DE-588)4190826-0 gnd rswk-swf Molekularer Motor (DE-588)4720488-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zilie (DE-588)4190826-0 s Dynein (DE-588)4236074-2 s Strukturanalyse (DE-588)4183787-3 s DE-604 Molekularer Motor (DE-588)4720488-6 s Genregulation (DE-588)4122166-7 s King, Stephen M. Sonstige (DE-588)141104457 oth (DE-604)BV035961549 2 Methods in cell biology 92 (DE-604)BV002534878 92 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855717&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cilia Methods in cell biology Genregulation (DE-588)4122166-7 gnd Strukturanalyse (DE-588)4183787-3 gnd Dynein (DE-588)4236074-2 gnd Zilie (DE-588)4190826-0 gnd Molekularer Motor (DE-588)4720488-6 gnd |
| subject_GND | (DE-588)4122166-7 (DE-588)4183787-3 (DE-588)4236074-2 (DE-588)4190826-0 (DE-588)4720488-6 (DE-588)4143413-4 |
| title | Cilia |
| title_auth | Cilia |
| title_exact_search | Cilia |
| title_full | Cilia [2] Motors and regulation ed. by Stephen M. King ... |
| title_fullStr | Cilia [2] Motors and regulation ed. by Stephen M. King ... |
| title_full_unstemmed | Cilia [2] Motors and regulation ed. by Stephen M. King ... |
| title_short | Cilia |
| title_sort | cilia motors and regulation |
| topic | Genregulation (DE-588)4122166-7 gnd Strukturanalyse (DE-588)4183787-3 gnd Dynein (DE-588)4236074-2 gnd Zilie (DE-588)4190826-0 gnd Molekularer Motor (DE-588)4720488-6 gnd |
| topic_facet | Genregulation Strukturanalyse Dynein Zilie Molekularer Motor Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018855717&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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| work_keys_str_mv | AT kingstephenm cilia2 |