Reverse chemical genetics: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Dordrecht [u.a.]
Humana Press
2009
|
| Schriftenreihe: | Methods in molecular biology
577 |
| Schlagworte: | |
| Online-Zugang: | Cover Inhaltsverzeichnis |
| Beschreibung: | XI, 272 S. Ill., graph. Darst. |
| ISBN: | 1607612313 9781607612315 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804140890715848704 |
|---|---|
| adam_text | Titel: Reverse chemical genetics
Autor: Koga, Hisashi
Jahr: 2009
Contents
Preface v
Contributors. ix
Part I The Importance of Fully-Characterized Genomic
Resources and Informatics for Reverse Chemical Genetics
1. ORFeome Cloning 3
Osamu Ohara
2. Systematic Cloning of an ORFeome Using the Gateway System 11
Akihisa Matsuyama and Minoru Yoshida
3. High-Throughput Construction of ORF Clones
for Production of the Recombinant Proteins 25
Hisashi Yamakawa
4. Computational Overview of GPCR Gene Universe to Support
Reverse Chemical Genomics Study 41
Makiko Suwa and Yukiteru Ono
5. High-Performance Gene Expression Module Analysis
Tool and Its Application to Chemical Toxicity Data 55
Wataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada,
Takeaki Tanijjuchi, and Hideko Sone
6. Human Protein Reference Database and Human
Proteinpedia as Discovery Tools for Systems Biology 67
T.S. Keshava Prasad, Kumaran Kandasamy, andAkhilesb Pandey
Part II Modification of cDNA Resources Utilized
for Reverse Chemical Genetics
7. High-Throughput Production of the Recombinant Proteins
Expressed in Escherichia colt Utilizing cDNA Resources 83
Kiyo Shimada and Hisashi Koga
8. An Insect Cell-Free System for Recombinant Protein Expression
Using cDNA Resources 97
Takashi Suzuki, Toru Ezure, Masaaki Ito, Masamitsu Shikata,
and Eiji Ando
9. Recombinant Protein Production by a Kaiko-Baculovirus System 109
Hidekazu Najjaya
10. Pulse-Chase Experiment for the Analysis of Protein
Stability in Cultured Mammalian Cells by Covalent
Fluorescent Labeling of Fusion Proteins 121
Kei Yamagucbi, Shinichi Inoue, Osamu Ohara, and Takahiro Naj/ase
vii
viii Contents
Part III The Refinement of Established Methods
by Recent Innovations
11. Application of 2D-DIGE in Cancer Proteomics
Toward Personalized Medicine 135
Tadashi Kondo and Setsuo Hirohashi
12. Fully Automated Two-Dimensional Electrophoresis System
for High-Throughput Protein Analysis 155
Atsunori Hiratsuka and Kenji Tokoyama
13. Large-Scale Whole Mount In Situ Hybridization
of Mouse Embryos 167
Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asabara
14. Identification of the Specific Binding Proteins of Bioactive
Small Compound Using Affinity Resins 181
Akito Tanaka
Part IV Potential Applicable Techniques for Reverse
Chemical Genetics
15. Protein Microarrays: Effective Tools for the Study
of Inflammatory Diseases 199
Xiaobo Til, Nicole Schneiderhan-Marra, Hsin-Yun Hsu,
Jutta Bachmann, and Thomas O. Joos
16. Preparation of Highly Sensitive Protein Array Using
Reactive Polymer 215
Toshifumi Shiroya, Hiroyuki Tanaka, Minako Hanasaki,
and Hisao Takeuchi
17. High-Throughput SPR Biosensor 227
Motoki Kyo, Kimihiko Ohtsuka, Eiji Okamoto, and Kazuki Inamori
18. Drug Discovery Through Functional Screening
in the Drosophila, Heart 235
Takeshi Akasaka and Karen Ocorr
19. Permeable Cell Assay: A Method for High-Throughput
Measurement of Cellular ATP Synthetic Activity 251
Kiyotaka Y. Hara
20. Novel Methodology for Immobilization of Biomolecules on the Surface
of a Photoresponsible Polymer Containing Azobenzene Moiety 259
Osrnnu Watanabe
Index 269
|
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| indexdate | 2024-07-09T22:07:10Z |
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| isbn | 1607612313 9781607612315 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Reverse chemical genetics methods and protocols ed. by Hisashi Koga Dordrecht [u.a.] Humana Press 2009 XI, 272 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 577 Springer protocols Aktivität (DE-588)4141766-5 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Chemische Biologie (DE-588)7657972-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Chemische Biologie (DE-588)7657972-4 s Proteine (DE-588)4076388-2 s Aktivität (DE-588)4141766-5 s b DE-604 Koga, Hisashi edt Methods in molecular biology 577 (DE-604)BV035362695 577 DE-576;springer image/jpeg http://swbplus.bsz-bw.de/bsz312918097cov.htm 20091112085620 Cover HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018765250&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Reverse chemical genetics methods and protocols Methods in molecular biology Aktivität (DE-588)4141766-5 gnd Proteine (DE-588)4076388-2 gnd Chemische Biologie (DE-588)7657972-4 gnd |
| subject_GND | (DE-588)4141766-5 (DE-588)4076388-2 (DE-588)7657972-4 (DE-588)4143413-4 |
| title | Reverse chemical genetics methods and protocols |
| title_auth | Reverse chemical genetics methods and protocols |
| title_exact_search | Reverse chemical genetics methods and protocols |
| title_full | Reverse chemical genetics methods and protocols ed. by Hisashi Koga |
| title_fullStr | Reverse chemical genetics methods and protocols ed. by Hisashi Koga |
| title_full_unstemmed | Reverse chemical genetics methods and protocols ed. by Hisashi Koga |
| title_short | Reverse chemical genetics |
| title_sort | reverse chemical genetics methods and protocols |
| title_sub | methods and protocols |
| topic | Aktivität (DE-588)4141766-5 gnd Proteine (DE-588)4076388-2 gnd Chemische Biologie (DE-588)7657972-4 gnd |
| topic_facet | Aktivität Proteine Chemische Biologie Aufsatzsammlung |
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