Pharmaceutical bioassays: methods and applications
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Hoboken, N.J.
J. Wiley
2009
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references |
| Beschreibung: | XXIII, 497 S. Ill., graph. Darst. |
| ISBN: | 9780470227602 |
Internformat
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| 245 | 1 | 0 | |a Pharmaceutical bioassays |b methods and applications |c Shiqi Peng ... [et al.] |
| 264 | 1 | |a Hoboken, N.J. |b J. Wiley |c 2009 | |
| 300 | |a XXIII, 497 S. |c Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Includes bibliographical references | ||
| 650 | 4 | |a Drugs |x Testing | |
| 650 | 4 | |a Biological assay | |
| 650 | 4 | |a Biological Assay |x methods | |
| 650 | 4 | |a Pharmaceutical Preparations |x analysis | |
| 700 | 1 | |a Peng, Shiqi |e Sonstige |4 oth | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018742442&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804140866764275712 |
|---|---|
| adam_text | Titel: Pharmaceutical bioassays
Autor: Peng, Shiqi
Jahr: 2009
CONTENTS
Preface xxi
Contributors xxiii
1 Methods and Applications of Anticancer Bioassays / 1
Shiqi Peng
1.1 MTT Assay for Six Carcinoma Cells / 2
1.2 Flow Cytometric Assay for Cell Apoptosis / 3
1.3 DNA Fragmentation Assay / 3
1.4 Bcl-XL/BH3 Interaction Assay / 3
1.5 Dissociation-Enhanced Lanthanide Fluoro-Immunoassay
(DELFIA) / 4
1.6 Ishikawa Cell and Rat Assay for Detecting Antiestrogens / 5
1.7 ATP Assay for Eight Cells / 7
1.8 AP Activity Assay / 7
1.9 Tumor Endothelial Cell Tube Formation Assay / 8
1.10 Antiangiogenic Assay / 9
1.11 In Vivo Hollow Fiber Assay / 10
1.12 VX2 Rabbit Lung Assay / 11
1.13 Insulin-Like Growth Factor-I-Induced Kinase Receptor
Activation Assay / 11
1.14 Insulin-Like gD.trkA-Induced Kinase Receptor Activation Assay / 12
1.15 UV Spectra-Based Calf Thymus DNA Intercalation Assay / 12
1.16 Fluorescence Spectra-Based Calf Thymus DNA
Intercalation Assay / 13
1.17 P-Glycoprotein Pump in MCF-7R Cells Assay / 13
1.18 P-Glycoprotein Pump-Related Efflux Carriers Assay / 14
1.19 [3H]Substrate Transport Inhibition Assay / 15
1.20 Lactate Dehydrogenase Release Assay / 15
1.21 Functional Assay of Mitochondrial P-gp / 16
1.22 Resistance Index Value Assay / 16
References and Notes / 17
v
Vi CONTENTS
2 Methods and Applications of Antiviral Assays / 23
Shiqi Peng, Ming Zhao, and Chunying Cui
2.1 Nonradioactive HIV-1 RT Activity Assay / 24
2.2 Respiratory Syncytial Virus Assay / 24
2.3 Influenza Virus Types A and B Assay / 24
2.4 Nasal Exhaled NO Concentration Assay / 25
2.5 Nasal NOS2 mRNA Quantity Assay / 25
2.6 RT-PCR and Swine Assay for Anti-HEV Antibody / 26
2.7 HIV-1 Protease and Reverse Transcriptase Kinetic Assay / 26
2.8 Anti-HIV Assay / 27
2.9 Robust Antiviral Assays / 28
2.10 HIV/SIV Fusion Assay / 28
2.11 Rapid DNA Hybridization Assay / 29
2.12 Antiviral Screening Assay for HepAD38 Cell Cultures / 29
2.13 Trak-C HCV Core Assay / 31
References and Notes / 33
3 Methods and Applications of Antitubercular Assays / 39
Shiqi Peng, Ming Zhao, and Chunying Cui
3.1 Mycobacterium tuberculosis Assay / 39
3.2 DNA Polymerase (3 Lyase Assay / 40
3.3 Agar Dilution Assay for In Vitro Antitubercular Activity / 41
3.4 Microplate Alamar Blue Assay for In Vitro Antitubercular
Activity / 41
3.5 Radiometric Respiratory Assay for In Vitro Antitubercular
Activity / 42
3.6 Mycobacterium bovis BCG Inhibition Assay / 42
References and Notes / 43
4 Methods and Applications of Thrombus-Related Assays / 45
Ming Zhao
4.1 In Vitro Anti-Platelet Aggregation Assay / 46
4.2 Fibrinolytic Area Assay / 47
4.3 TXB2 and PGD2 TLC Assay / 47
4.4 TXA2 Synthase Activity Assay / 48
4.5 [Ca2+]; Measuring Assay / 48
4.6 Arachidonic Acid Liberation Assay / 48
4.7 Serotonin Secretion Assay / 48
4.8 cAMP Release Assay / 49
4.9 Ex Vivo Anti-Platelet Aggregation Assay for Patients / 49
4.10 ATP Release Assay / 50
4.11 PAF-Induced Mice Mortality Assay / 50
CONTENTS Vii
4.12 PGE2 and TXB2 ELISA / 50
4.13 Thrombelastograph Assay / 51
4.14 Image-Monitored FeCl3-Induced Thrombosis Assay for Rat / 51
4.15 Weight-Monitored FeCl3-Induced Thrombosis Assay for Rats / 52
4.16 Occlusion Time-Monitored FeCl3-Induced Thrombosis
Assay for Pig / 52
4.17 Doppler Blood Flow-Monitored FeCl3-Induced Thrombosis
Assay for Mouse / 53
4.18 Rat Groin Flap Assay / 54
4.19 Ferret Acute Thrombosis Assay / 55
4.20 Rat Acute Thrombosis Assay / 56
4.21 Arteriovenous Shunt Assay / 56
4.22 Plasma Clotting Time Assay / 56
4.23 Thromboembolic Photochemical Assay for Repeated
Stroke in Mice / 57
4.24 Euglobulin Clot Lysis Assay / 58
4.25 Clot Formation and Lysis (CloFAL) Assay / 59
4.26 Fibrin Microplate Assay / 59
4.27 Fibrinolytic Activity Assay / 60
4.28 Fibrinolysis Assay / 60
4.29 Thrombolytic Assay / 61
References and Notes / 62
5 Methods and Applications of Anticoagulation Assays / 65
Ming Zhao
5.1 Ecarin Chromogenic Assay / 66
5.2 Anticoagulation Activity Assay in an In Vitro System / 67
5.3 Anticoagulation Activity Assays in an In Vivo System / 67
5.4 Rat Thrombosis Assay / 68
5.5 In Vivo Microvascular IVC Blood Flow Assay / 68
5.6 OwrenPT Assay / 68
5.7 Rabbit Double-Balloon Injury Assay / 69
5.8 A Rapid Point-of-Care Assay for Enoxaparin / 70
5.9 Thromboplastin Clotting Assay / 71
5.10 Rat Assay for Reproducible Stasis-Induced Venous Thrombosis / 71
5.11 In Vivo ACT II/Ecarin Clotting Time Assay / 72
5.12 Ex Vivo and In Vivo Anticoagulation Assay / 73
5.13 Plasma-Based Ecarin Clotting Time Assay for r-Hirudin / 74
5.14 Protamine Titration for Heparin in Whole Blood / 75
5.15 Automated Assay Evaluating Response of Kaolin ACT
to Heparin / 76
5.16 Platelet/Monocyte Interaction-Based PM Exposure
Mouse Assay / 77
Viii CONTENTS
5.17 Mouse Tail-Bleeding Time Assay / 78
5.18 MRI Assay for Rabbit Atherosclerotic Lesions / 78
5.19 Spiral Computed Tomography Assay / 79
5.20 Duplex Ultrasound Assay / 80
5.21 Standard Hemochron Assay / 81
5.22 Platelet Serotonin Release Assay / 81
5.23 Activated Partial Thromboplastin Time Assay / 82
5.24 Rat Stroke Outcome Assay / 82
References and Notes / 83
6 Methods and Applications of Blood Pressure-Related Assays / 87
Ming Zhao
6.1 Human Plasma New Pressor Protein Assay / 88
6.2 Pulmonary Hypertension Assay / 89
6.3 Coronary Arteries Constriction Assay / 90
6.4 MRI and Brain Natriuretic Peptide Assays / 90
6.5 Competition Enzyme-Linked Immunosorbent Assay / 91
6.6 Right Ventricular Pressure Assay / 92
6.7 Plasma Nitrite/Nitrate Concentration Assay / 92
6.8 Adeno-Associated Virus Vector-Caused Rat Pulmonary Artery
Pressure Assay / 92
6.9 Adeno-Associated Virus Vector-Caused Rat Protein and mRNA
Assay / 93
6.10 N-Terminal pro-Brain Natriuretic Peptide (N-T proBNP) Assay / 93
6.11 Middle Cerebral Artery Occlusion Assay / 94
6.12 Vascular Endothelial Growth Factor Level Assay / 95
6.13 Antispasmodic Agent In Vivo Action Assay / 95
6.14 Temperature Assay in Awake Subjects / 96
References and Notes / 97
7 Methods and Applications of Assays Related to Parkinson s
Disease and Graves Disease / 101
Shiqi Peng
7.1 Flow Cytometric Assay for Cellular DNA Content and
Caspase-3 / 102
7.2 Swine Resuscitation Assay / 103
7.3 HTLV-taxl or pMuLV-SV-nlslacZ Vectors Transfected
Cell Assay / 104
7.4 Parkinsonian Rat Assay / 104
7.5 ELISA for Nerve Growth Factor Antigen / 105
7.6 Assay for P-Nerve Growth Factor Levels in Cerebrospinal
Fluid / 106
CONTENTS JX
7.7 MDCK Scatter Assay / 107
7.8 Facial Nerve and Spinal Root Avulsions / 108
7.9 TRAb Assays / 108
7.10 Human Thyrotropin Receptor (hTSHR) Assay / 109
7.11 Soluble ICAM-1, TSAb, and TBIAb Activity Assays /111
7.12 Microarray Immunoassay for hTSHr Production / 112
7.13 Affinity Assay for [35S]GTP7S Binding to Gas/olf / 113
7.14 Tissue Segment Binding Assay fora, B-Adrenoceptor / 114
7.15 Membrane Binding Assay with Rat Cerebral Cortex for
aie-Adrenoceptor / 115
7.16 Whole Cell Binding Assay for a1B-Adrenoceptor / 115
7.17 Functional Assay for a1AB-Adrenoceptor / 116
7.18 Rat Neuroprotective Assay / 116
7.19 GABA-Benzodiazepine Receptor Assay / 117
7.20 Forced Swimming and Tail Suspension Assays / 117
7.21 IGF-I Kinase Receptor Activation (KIRA) Assay / 118
7.22 gD.trkA Kinase Receptor Activation (KIRA) Assay / 118
7.23 gD.trk KIRA-ELISA / 118
7.24 Assays for Cannabinoid Receptors in Rat Cerebella or Mouse
Brains / 120
References and Notes / 121
8 Methods and Applications of Alzheimer s Disease Assays / 125
Shiqi Peng
8.1 Assay for Oxidative Stress in Cerebral Cortex of AD Mice / 126
8.2 Reporter Assay for Primary Neuronal Cultures / 127
8.3 Electrophoretic Mobility Shift Assay (EMSA) / 127
8.4 Binding Assays Using Aggregated AfS Peptide in Solution / 127
8.5 Assay for Muscarinic Receptor 1 in Alzheimer s
Dementia Model / 128
8.6 (3-Secretase Activity Assay / 128
8.7 AP Fibril Binding Assay / 129
8.8 TLC and Microplate Assays for Acetylcholinesterase
Inhibitors / 129
8.9 Immunocapture Assay Measuring Specific Enzyme Activity
ofNeprilysin / 130
8.10 In Vivo AChE Inhibition Assay / 131
8.11 Single Particle Assay for A|3 Aggregates / 132
8.12 Indirect Immunofluorescence Assay / 133
8.13 Mouse Behavioral Assays / 133
8.14 Center of Pressure (CoP) Assay in Mice / 135
8.15 Assay for Plasma Levels of DJ-1 / 136
8.16 Membrane Filter Assay for Tau Aggregation / 137
X CONTENTS
8.17 Assays for Motor Neuron Degeneration / 138
8.18 HPLC Assay for Neuroprotective Agent in Mice Plasma / 140
8.19 Tissue Culture Assays for SOD 1 Mutations / 141
8.20 Luciferase-Based Reporter Assay / 142
References and Notes / 142
9 Methods and Applications of Antiosteoporosis Assays / 145
Ming Zhao
9.1 Rat Bone Mineral Density Assay / 146
9.2 Osteoblastic Cell Proliferation and Alkaline Phosphatase (ALP)
Activity Assays / 146
9.3 Murine Osteoblastic MC3T3-E1 Cell Calcification and
Van Kossa Assays / 147
9.4 Osteoclast Generation Assay for Male Senile Rat / 148
9.5 Bone Resorption and Recovery Related Assays / 148
9.6 Mouse Bone Mineral Density Assay / 149
9.7 PPAR-7 Competitor Assay / 150
9.8 Human Serum Estrogen Level Assay / 150
9.9 Luciferase Activity Assay / 150
9.10 IL-lp and TNF-a Level Assay / 150
9.11 ER Binding and Receptor Activity Assays / 151
9.12 Fluorescent Estrogen Receptor Assay / 152
9.13 ELISA for Urinary Helical Peptide / 153
9.14 Urine Midmolecule Osteocalcin Assay / 153
9.15 BMD and Osteocalcin Assay / 154
9.16 In Vivo Antiosteoporosis Assay on Mice / 154
References and Notes / 155
10 Methods and Applications of Immunomodulating Assays / 157
Shiqi Peng
10.1 Rat Mast Cell Histamine-Release Assay / 158
10.2 Rabbit Aortic Force Assay / 159
10.3 Dopaminergic Cell Death-Based Neural Transplantation
Assay / 159
10.4 Mast Cell Degranulation Assay / 160
10.5 Basophils Assay as Allergen / 161
10.6 RBL-2H3 Cell Desensitization Assay / 162
10.7 Migration Assay of Dendritic Cell from PBMCs / 162
10.8 Mouse EAE Induction Assay / 163
10.9 COSTIM Assay for DC/T-Cells / 163
10.10 Cytokine Assay for IL-6, IL-10, IL-12, and
TNF-aofDCs / 164
10.11 ELISPOT Assay for DC IFN--y / 165
CONTENTS Xi
10.12 DC Function Assay for Evaluating Toll-like Receptor
Function / 166
10.13 Migration Assay of Dendritic Cell from Bone Marrow of
A/JMice / 167
10.14 Lymphoid Organ Assay / 168
10.15 ELISA of IFN-7 from Human Myelomonocytic KG-1 Cells / 170
10.16 Human Whole Blood IFN-7 Assays / 171
10.17 Sheep Whole Blood IFN-7 Assays / 171
10.18 ELISPOT Assay for IFN-7 / 172
10.19 IFN-3RG Assay / 173
10.20 Anti-rHuEPO NAb Assay / 173
10.21 Chloramphenicol Acetyltransferase Assay / 174
10.22 Chemotaxis Assay / 175
10.23 Fibroblast-Populated Microsphere Assay / 175
10.24 Fibroblast-Populated Concentric Microsphere Assay / 176
10.25 Radial Assay of Chemotaxis / 178
10.26 Antibody Forming Cell Assay / 179
10.27 Immunosuppressive Assay / 179
10.28 Cell-Based ELISA / 180
10.29 Large Animal Lung Transplantation Assay / 181
References and Notes / 182
11 Methods and Applications of Anti-Inflammatory Assays / 187
Ming Zhao
11.1 Adhesion Formation Assay / 188
11.2 Ligand Complex-Based Adhesion Assay / 189
11.3 Human Umbilical Vein Endothelial Cell Assay / 190
11.4 Pleurisy Mouse Assay / 191
11.5 Proliferation of PBMC Assay / 192
11.6 COX-1, COX-2, and 5-LOX Assay with [ 1 - 14C]Arachidonic
Acid / 193
11.7 COX-1 and COX-2 Assay with Human Whole Blood / 193
11.8 o-Hydroxyleukotriene B4 Assay / 194
11.9 Leukocyte Rolling and Adherence Assay / 194
11.10 CCR5 Receptor Binding Assay / 195
11.11 Tissue Binding Affinity Assay / 195
11.12 G93A-SOD1 Transgenic Mouse Assay / 196
11.13 MCP-1-Induced ERK1 and ERK2 Phosphorylation Assay / 197
11.14 LPS- and IL-6-Induced ERK1 and ERK2 Phosphorylation
Assay / 198
11.15 ELA4.NOB-1/CTLL Cell Assay / 199
11.16 FK506 Binding Protein 51 (FKBP51) mRNA Assay / 200
11.17 Xylene-Induced Ear Edema Assay / 201
References and Notes / 201
Xii CONTENTS
12 Methods and Applications of Antioxidant Activity Assays / 205
Shiqi Peng
12.1 Blood and Plasma Total Antioxidant Capacity (TAC) Assay / 206
12.2 Ferric Reducing-Antioxidant Power (FRAP) Assay / 207
12.3 Human LDL Oxidation Assay / 207
12.4 DPPH Radical Cation Scavenging Assay / 207
12.5 ABTS+Radical Cation Scavenging Assay / 208
12.6 Lipid Peroxidation Assay Using Rat Brain Tissue / 208
12.7 Flow-Through Chemiluminescence (FTCL) Assay / 209
12.8 Superoxide Radical Scavenging Assay / 209
12.9 Deoxyribose Assay for Hydroxyl Radical Scavenging
Activity / 209
12.10 DNA Nicking Assay for Hydroxyl Radical Scavenging
Activity / 210
12.11 Oxidative Lag-Time Assay / 210
12.12 TBARS and Electrophoresis Assay / 210
12.13 Reporter and Electrophoretic Mobility Shift Assay (EMS A) / 211
12.14 [Ca2+]cyt Assay / 211
12.15 Quantitative Real-Time PCR Assay / 212
12.16 FT-IR-Based Assay for Antioxidation Activity of Ionol and
Piperidone / 212
12.17 HPLC Assay for Antioxidation Potential of Polyphenol / 213
12.18 ROS Production Assay / 213
12.19 Rabbit LDL Oxidation Assay / 213
12.20 ROS Scavenging Assays / 214
12.21 DNA Damage Assay / 215
12.22 Egg Yolk TBARS Assay / 216
12.23 Mouse Catalase (CAT) Assay / 216
12.24 Rat Tissue TBARS Assay / 216
12.25 Antioxidant Activity Assay for P-Carotene/Linoleic
Acid System / 217
12.26 Rat Brain Tissue NO Assay / 217
12.27 Rat Brain Antioxidative Enzyme Assay / 218
12.28 Rat Brain Hippocampi Protein Oxidation Assay / 218
References and Notes / 219
13 Methods and Applications of Analgesic Assays / 223
Ming Zhao
13.1 Algogenic Activity Assay for Rat Ureteral Stone / 224
13.2 Cold Pressor-Based Assay for Acute Pain of Healthy
Volunteers / 224
13.3 Heat-Based Assay for Acute Pain of Healthy Volunteers / 225
13.4 Electrical Stimulation-Based Assay for Acute Pain of Healthy
Volunteers / 225
CONTENTS Xiii
13.5 Radiant Heat Tail-Flick Assay for Mice / 226
13.6 Pain Behaviors/Responses Assays in Rats / 226
13.7 Hot Plate Assay in Rats / 227
13.8 Plantar Assay in Rats / 227
13.9 Hot Plate Assay in Mice / 227
13.10 Paw and Tail Formalin Assays in Mice / 228
13.11 Rat Assays for Bone Cancer Pain / 229
13.12 Mouse Assay for Hindpaw Cancer Pain / 229
13.13 Visceral Pain Assay / 230
13.14 Canine Nociceptive Thermal Escape Assay in Dog / 230
13.15 Carrageenan Assay in Rats / 232
13.16 Electrophysiologic Assay for Mice with Tumor-Evoked
Hyperalgesia / 232
13.17 Mouse Assay for Bone Cancer Pain / 233
References and Notes / 234
14 Methods and Applications of Epilepsy Assays / 237
Ming Zhao, Shiqi Peng, and Guohui Cui
14.1 HISS Assay / 238
14.2 Mouse Locomotor Activity Assay / 239
14.3 Mouse Diathesis-Stress Assay / 240
14.4 Social Interaction Assay / 240
14.5 Spatial Learning Ability Assay for Recurrent Seizure Rats / 241
14.6 Timed PTZ Infusion Assay for Mice / 241
14.7 Timed PTZ Seizure Assay for Mice / 242
14.8 Maximal Electroconvulsions Threshold Assay for Mice / 243
14.9 MES Assay / 243
14.10 6-Hz Psychomotor Seizure Assay for Mice / 244
14.11 Subcutaneous Bicuculline and Picrotoxin Assay for Mice / 244
14.12 NMDA-Induced Convulsions Assay for Mice / 244
14.13 AGS Assay / 245
14.14 Kindled Rat Assay for Focal Seizures / 245
14.15 Cobalt/Homocysteine Assay for Status Epilepticus
of Rats / 246
14.16 PTZ-Induced Kindling Assay / 247
14.17 ICES Assay for Mice / 247
References and Notes / 248
15 Methods and Applications of Diabetes Assays / 251
Ming Zhao, Shiqi Peng, and Guohui Cui
15.1 Islet Xenograft Assay for Diabetic Mice / 252
15.2 Assays for Spontaneous Diabetes and Adoptive Transfer of
Diabetes / 252
XIV CONTENTS
15.3 Oral Glucose Tolerance Assays for Patients / 253
15.4 Injection Glucose Tolerance Assays for Rats / 253
15.5 Renal Cortical TGF-01 Protein Assays for Rats / 254
15.6 Low-Dose Streptozotocin-Treated Heminephrectomized
Rat Assay / 254
15.7 Rat Early Diabetic Nephropathy Assay / 255
15.8 Urinary Endothelin-1 Excretion Assay for Type 2
Diabetes Rats / 255
15.9 Subtotally Nephrectomized Rat Assay / 256
15.10 Type 2 Diabetes Mice Assay / 256
References / 257
16 Methods and Applications of Assays for Toxins
from Microorganisms / 259
Shiqi Peng
16.1 Colorimetric Yeast Assay for Trichothecene
Mycotoxins / 260
16.2 Colorimetric Cell Proliferation Assay / 261
16.3 Yeast DEL Assay / 262
16.4 NCCLS and EUCAST Assays / 263
16.5 Alcohol Dehydrogenase-Based Colorimetric Assays / 264
16.6 Colorimetric Assay for Iron in Yeast / 265
16.7 Microplate Redox Assays of E. coli / 265
16.8 Ciliate Tetrahymena thermophila Assay for Trichothecene
Mycotoxins / 266
16.9 Fluorescent Dyes-Based Cell Viability Assay for Triton X-100
Toxicity / 267
16.10 MTT Assay for Fusarium Mycotoxins / 268
16.11 Mortality and Frass Production Assay for Toxicity of Bacterial
Strains / 269
16.12 Cell Toxicity Assay / 270
16.13 Dhase Inhibition Assay / 271
16.14 Sediment Toxicity Assay / 271
16.15 Toxicity Assay of Particle-Associated Arsenite
and Mercury / 272
16.16 Genetic Toxicity Assay / 273
16.17 Microtox Assays / 275
16.18 DNA Piezoelectric Biosensor Assay / 275
16.19 Protein Phosphatase Inhibition Assay and ELISA
of Microcystins / 276
16.20 Lepidium sativum Assay for Microcystin Toxicity / 277
16.21 Antiproliferative Assay for Interleukin-4 / 278
References and Notes / 278
CONTENTS XV
17 Methods and Applications of Toxicity Assays for Chemicals / 281
Shiqi Peng
17.1 Lux-Fluoro Assay for Combined Genotoxicity and Cytotoxicity
of Chemicals / 282
17.2 How Cytometry and Microscopy Based Assay / 283
17.3 Cell Transformation Assay / 284
17.4 ALIC-Based Assay for Toxicity of Chemicals in SPM / 284
17.5 Immunization in the Murine Assay / 286
17.6 Immunotoxicological Functional Assay / 286
17.7 PWM-Induced IgM Assay for Toxicity of Chemicals / 288
17.8 Tetrachlorodibenzo-p-dioxin (TCDD)-Induced Toxicity Assay / 289
17.9 H4IIE EROD Assay / 291
17.10 Rat Mutation Assay / 291
17.11 AhR and Related Assay / 292
17.12 Ectonucleotidase Expression Assay / 293
17.13 Toxicity Equivalent Quantity (TEQ) Assay / 296
17.14 Enzyme Lacking A A Epoxygenase Activity Assay / 297
17.15 Japanese Medaka Embryo-Larval Assay / 301
17.16 Green Fluorescent Protein-Based Cell Assay / 302
17.17 RACB Assay for Ovarian Toxicity of Xenobiotics / 303
17.18 Uroepithelial Cell Assay / 304
17.19 Male Rat Systemic Toxicity Assay / 306
17.20 28-Day Oral Toxicity Assay / 308
17.21 Endocrine Disruptors Assay / 310
17.22 Mating Efficiency Assay / 310
17.23 Soils Assay for Contaminants and Toxicity / 311
17.24 Rice Nitrate Reductase Activity Assay / 313
17.25 Tradescantia-Micronucleus Assay / 314
17.26 Nitric Oxide Neurotoxicity Assay / 314
17.27 EBV-Transformed Human Burkitt s Lymphoma Cell Assay / 315
17.28 Murine Bone Marrow Assay for Hemopoietic and Osteogenic
Toxicity / 316
17.29 PNAR-GFP Assay for Carcinogenic Toxicity of Nitrate / 317
17.30 Brine Shrimp (Artemia salina) Assay / 318
17.31 DPPH Radical Scavenging Property Assay / 318
References and Notes / 319
18 Methods and Applications of Hepatoxicity and Hepatoprotective
Assays / 325
Shiqi Peng
18.1 GST-P Enzyme-Altered Foci Assay / 326
18.2 GST-P+and TGF-a+Foci Assay / 327
XVi CONTENTS
18.3 Partial Hepatectomy Assay / 327
18.4 In Vivo Short-Term Liver Initiation Assay in Rats / 328
18.5 TUNEL Related Assay / 328
18.6 Hematopoietic Progenitor Cells Mobilization Assay / 330
18.7 Electrophoretic Mobility Shift Assay / 331
18.8 Telomerase Activity Assay / 332
18.9 Von Willebrand Factor (vWF) Level Assay / 333
18.10 Bromodeoxyuridine Incorporation Assay / 333
18.11 NF-kB Activation Assay / 334
18.12 Rat Summation of Initiation Activity Assay / 335
18.13 Hepatoprotective Assay / 335
18.14 Cytokine Gene Expression Assay / 336
18.15 Competitive Inhibition Assay for Immunodominance of
O-Specific Polysaccharides of Gram-Negative Bacilli / 337
18.16 Marine HP Toxin Okadaic Acid (OA) Assay and ELISA / 338
18.17 Anti-GST-CTX MVIIA Antibody in Omega-Conotoxin
MVIIA Assay / 338
18.18 Assays for Rituximab in Patient Plasmas / 340
18.19 Assays of TNF-a, Superoxide and Thymocyte for Liver Injury
of Mice / 341
18.20 Gene Transfer of Kringle 1-5 and Related Assays for Mice with
Hepatocellular Carcinoma / 343
18.21 Body Weight Assay for Mice with Liver Tumor
Incidence / 345
18.22 P450 Activity and Inducibility in Rat Hepatocytes for
Cytotoxicity Assay / 346
18.23 LDH Release in Rat Hepatocytes for Toxicity Assay / 348
18.24 Rainbow Trout Hepatocytes EROD Activity Assay / 349
18.25 EROD Activity, ROS Production and Cytotoxic Concentration in
Fish Hepatocytes for Drug Toxicity Assays / 349
18.26 Coho Salmon s IGF-I Gene Sequencing and TaqMan Assay / 350
18.27 DNA Strand Break in Rat Hepatocyte and Comet Assay / 352
18.28 DNA Laddering in Rat Hepatocyte and TUNEL Assay / 353
18.29 Counting Increases in Viable Cell Number for Hepatocyte
Proliferation Assay / 354
18.30 Assay of HGF and TGF-0 in CCL-64 Cells / 356
18.31 Assay for Serum EFN-a of Patients with Chronic Hepatitis C / 356
18.32 Rat Medium-Term Liver, DNA Microarray and Cu+-Reducing
Antioxidation Assays for DEN-Induced Hepatocarcinogenesis / 358
18.33 Rat Medium-Term Liver Assay for DEN-Induced
Hepatocarcinogenesis Assay / 360
18.34 Assay for Rat Carcinogenesis Initiated by Three Carcinogens / 361
18.35 Assay for Rat Carcinogenesis Initiated by Five Carcinogens / 362
18.36 Assay for DEN-Initiated Rat Carcinogenesis / 363
CONTENTS XVii
18.37 Assay for Normal Dose DEN-Initiated and Low Dose
DEN-Maintained Rat Carcinogenesis / 363
18.38 TGF-|3-Mediated Antiproliferation Assay / 364
18.39 Assay for DEN and HCB-Initiated Rat Carcinogenesis / 365
References and Notes / 365
19 Methods and Applications of Estrogen Assays / 373
Shiqi Peng
19.1 Yeast Estrogen Assay / 374
19.2 In Vivo, Ex Vivo, and In Vitro Assays for Estrogen-Like
Effect / 375
19.3 Ameliorative Yeast Assay / 376
19.4 In Yeast Two-Hybrid Assay / 377
19.5 Estrogen-Sensitive Yeast Strain RMY/ER-ERE Assay / 378
19.6 Bioluminescent Yeast Assays / 379
19.7 LYES Assay / 380
19.8 ERa and ERfJ Stable Transactivation Assay / 381
19.9 HELNa and HELNP Transfected Cell Assay / 382
19.10 Luciferase Reporter Gene Assay / 382
19.11 Target AKT Pathway Assay / 383
19.12 Luciferase Assay / 384
19.13 Androgen Receptor Transactivation Assay / 385
19.14 Green Fluorescent Protein Expression Assay / 387
19.15 Luteinizing Hormone Releasing Hormone (LHRH) Assay / 388
19.16 Ishikawa Endometrial Cancer Cell Assay for Estrogen
Receptor Expression / 390
19.17 Ishikawa Cell Assay for 11 (3-HSD2 Activity / 390
19.18 Ishikawa Cell Assay for Estrogen Activity / 391
19.19 Ishikawa Cell Assay for Plasminogen Activator
Inhibitor-1 (PAI-1) / 391
19.20 Testosterone Induction Assay with Sertoli Cell / 392
19.21 Electrophoretic Mobility and Antibody Shift Assays / 392
References and Notes / 393
20 Methods and Applications of Antimalarial Assays / 397
Shiqi Peng
20.1 Plasmodium falciparum and Murine P388 Leukemia
Cell Assay / 398
20.2 Antiplasmodial Activity Assay / 398
20.3 Plasmodium falciparum Growth In Vitro Assay / 399
20.4 Antibody Assay for Red Blood Cell Polymorphisms / 400
20.5 Leishmania Macrophage Assay / 400
xviii contents
20.6 Lactate Dehydrogenase-Based Antiplasmodial Assay / 401
20.7 Histidine-Rich Protein II Assay / 401
20.8 Histidine-Rich Protein II Assay / 402
20.9 Antimalarial Activity Assay / 403
20.10 Plasmodium yoelii Liver Stage Parasites Inhibition Assay / 403
20.11 In Vivo Antimalarial Assay / 404
20.12 3-Hematin Inhibition Assay / 405
20.13 Non-radiolabeled Ferriprotoporphyrin IX Biomineralization
Inhibition Assay / 405
20.14 Survival of Anopheles gambiae Assay / 406
20.15 Chloroquine Assay / 407
20.16 MS Assay for Quantification of Chloroquine in
Dog Plasma / 407
20.17 Sensitive Fluorescence HPLC Assay for AQ-13 / 408
20.18 HPLC and HPTLC Assays for Chloroquine, Primaquine,
and Bulaquine / 409
20.19 Real-Time PCR-Based Chloroquine Sensitivity Assay / 410
20.20 Rat Embryos In Vitro Assay / 411
20.21 HPLC-MS Assay for (3-DHA in Rat Plasma / 413
20.22 Plasmodium falciparum Clone and DHA Assay / 414
References / 415
21 Methods and Applications of Cytogenetic Receptor and Enzyme
Assays / 419
Shiqi Peng
21.1 UT-7/EPO Cell Proliferation and Neutralizing Anti-EPO
Antibody Assays / 420
21.2 Assay System for Biotin Protein Ligase (BPL) from
Escherichia coli / 422
21.3 p-Galactosidase and a-Amylase Biosynthesis Assays / 422
21.4 Penicillin-Binding Protein (PBP 5) and Vancomycin Activity
Assays / 423
21.5 Recombinant Bacteria Assay Evaluating Androgen
Biosynthesis / 424
21.6 UDP-Glc 4-Epimerase Assay / 426
21.7 ATPs Exchange Assays / 426
21.8 Lux-Fluoro Assay for Combined Genotoxicity
and Cytotoxicity / 427
21.9 Reporter Gene Assay for Aryl Hydrocarbon Receptor / 427
21.10 CYP1A Enzyme Assay / 428
21.11 CYP1A Activity (EROD) Assay / 430
21.12 Deubiquitinating Enzyme (DUB) Activity Assay / 431
21.13 How Cytometric Assay / 432
21.14 Serum Neutralization Assay Based on rHMPV-GFP / 434
CONTENTS XJX
21.15 Yellow Fluorescent Protein-Based Assay / 435
21.16 Red Fluorescent Cytotoxic T Lymphocyte Assay / 437
21.17 Green Fluorescent Protein-Based Assay / 437
21.18 DNA Break Assay in HepG2 Cells / 439
References and Notes / 439
Index 445
|
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| id | DE-604.BV035884956 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-09T22:06:47Z |
| institution | BVB |
| isbn | 9780470227602 |
| language | English |
| lccn | 2009007433 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018742442 |
| oclc_num | 311059904 |
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| physical | XXIII, 497 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | J. Wiley |
| record_format | marc |
| spelling | Pharmaceutical bioassays methods and applications Shiqi Peng ... [et al.] Hoboken, N.J. J. Wiley 2009 XXIII, 497 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references Drugs Testing Biological assay Biological Assay methods Pharmaceutical Preparations analysis Peng, Shiqi Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018742442&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pharmaceutical bioassays methods and applications Drugs Testing Biological assay Biological Assay methods Pharmaceutical Preparations analysis |
| title | Pharmaceutical bioassays methods and applications |
| title_auth | Pharmaceutical bioassays methods and applications |
| title_exact_search | Pharmaceutical bioassays methods and applications |
| title_full | Pharmaceutical bioassays methods and applications Shiqi Peng ... [et al.] |
| title_fullStr | Pharmaceutical bioassays methods and applications Shiqi Peng ... [et al.] |
| title_full_unstemmed | Pharmaceutical bioassays methods and applications Shiqi Peng ... [et al.] |
| title_short | Pharmaceutical bioassays |
| title_sort | pharmaceutical bioassays methods and applications |
| title_sub | methods and applications |
| topic | Drugs Testing Biological assay Biological Assay methods Pharmaceutical Preparations analysis |
| topic_facet | Drugs Testing Biological assay Biological Assay methods Pharmaceutical Preparations analysis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018742442&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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