Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Verl.Dr. Hut
2009
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Biochemie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 111 S. Ill., graph. Darst. 210 mm x 148 mm, 201 gr. |
| ISBN: | 9783868532012 |
Internformat
MARC
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| 100 | 1 | |a Maurer, Ulrike Elisabeth |e Verfasser |0 (DE-588)139838287 |4 aut | |
| 245 | 1 | 0 | |a Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |c Ulrike Elisabeth Maurer |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a München |b Verl.Dr. Hut |c 2009 | |
| 300 | |a VII, 111 S. |b Ill., graph. Darst. |c 210 mm x 148 mm, 201 gr. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Biochemie | |
| 502 | |a München, Techn. Univ., Diss., 2009 | ||
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| 650 | 0 | 7 | |a Glykoproteine |0 (DE-588)4071905-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Membranfusion |0 (DE-588)4210049-5 |2 gnd |9 rswk-swf |
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| 689 | 0 | 2 | |a Glykoproteine |0 (DE-588)4071905-4 |D s |
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Datensatz im Suchindex
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| adam_text |
1 TABLE OF CONTENTS 1 TABLE OF CONTENTS 1 TABLE OF CONTENTS I 2
ABBREVIATIONS V 3 ZUSAMMENFASSUNG 1 4 ABSTRACT 3 5 INTRODUCTION 5 5.1
VIRAL FUSION PROTEINS G 5.2 HERPES SIMPLEX VIRUS 1 7 5.2.1 HERPESVIRUSES
7 5.2.2 HSV-1 STRUCTURE AND PROTEINS 8 5.2.3 HSV-1 LIFE CYCLE 13 5.2.4
HSV-1 ENTRY-ASSOCIATED GLYCOPROTEINS 14 5.2.4.1 GLYCOPROTEIN C 14
5.2.4.2 GLYCOPROTEIN D 14 5.2.4.3 GLYCOPROTEIN B 16 5.2.4.4
GLYCOPROTEINS H AND L 17 5.2.5 HSV-1 ENTRY 17 5.3 SEMLIKI FOREST VIRUS
20 5.4 TICK-BORNE ENCEPHALITIS VIRUS 21 5.5 CRYO ELECTRON MICROSCOPY AND
TOMOGRAPHY 22 5.6 AIM OF THE THESIS 22 6 MATERIALS AND METHODS 25 6.1
CELL CULTURE AND VIROLOGICAL TECHNIQUES 25 6.1.1 CELL CULTURE 25 6.1.2
VIRUSES 25 6.1.2.1 HERPES SIMPLEX VIRUS 1 26 6.1.2.2 SEMLIKI FOREST
VIRUS 27 6.1.2.3 TICK-BORNE ENCEPHALITIS VIRUS AND VIRUS-LIKE PARTICLES
27 6.1.3 LIGHT AND FLUORESCENCE MICROSCOPY 27 6.2 CHARACTERISATION OF
HSV-1 VIRUS PREPARATIONS 27 6.2.1 PLAQUE TITRATION OF HSV-1 28 6.2.2
SDS-PAGE 28 6.2.3 DETERMINATION OF THE GENOME/PFU RATIO 28 6.2.3.1
PURIFICATION OF VIRAL DNA 28 6.2.3.2 PLASMID PREPARATION 29 6.2.3.3
QUANTITAETEN OF DNA 29 6.2.3.4 PRIMER 29 6.2.3.5 PCR 30 6.2.3.6 AGAROSE
GEL ELECTROPHORESIS 30 6.2.3.7 REAL-TIME PCR 31 6. BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/998026905 DIGITALISIERT DURCH II 1 TABLE
OF CONTENTS 6.3.1 ISOTOPE-LABELLED STANDARD PEPTIDEA 31 6.3.2 SAMPLE
PREPARATION 32 6.3.3 NANOLC-MS/MS MEASUREMENTS 32 6.3.4 PEPTIDE
IDENTIFICATION AND QUANTITATION 33 6.4 GOLD LABELLING OF VIRAL
GLYCOPROTEINS 33 6.4.1 PROTEIN PREPARATION FOR LABELLING 33 6.4.1.1
ANTIBODIES 33 6.4.1.2 PROTEIN QUANTITATION 33 6.4.1.3 PREPARATION OF
FAB-FRAGMENTS 34 6.4.2 COLLOIDAL GOLD PARTICLES 34 6.4.3 CONJUGATION OF
IGGS OR FAB-FRAGMENTS TO GOLD 34 6.4.3.1 CONJUGATION OF IGGS OR
FAB-FRAGMENTS TO COLLOIDAL GOLD 34 6.4.3.2 CONJUGATION OF FAB-FRAGMENTS
TO NANOGOLD* 35 6.4.4 GOLD LABELLING OF NATIVE VIRIONS 35 6.4.4.1 VIRUS
LABELLING WITH FAB-FRAGMENTS COUPLED TO TANNIC ACID GOLD 35 6.4.4.2
VIRUS LABELLING USING A SECONDARY GOLD-COUPLED PROTEIN 35 6.4.4.3 GOLD
ENHANCEMENT AND SILVER ENHANCEMENT 36 6.5 LIPOSOMES 36 6.6 STRUCTURAL
ANALYSIS OF HSV-1 GB 36 6.6.1 FLOTATION ASSAY 37 6.6.2 LIPOSOME-PROTEIN
BINDING ASSAY 37 6.7 HOST SYSTEMS FOR VIRUS ENTRY 37 6.7.1 HSV-1
INOCULATION OF CELLS 38 6.7.2 HSV-1 INOCULATION OF SYNAPTOSOMES 38 6.7.3
HSV-1 INOCULATION OF LIPOSOMES 38 6.7.4 TBEV RSP-LIPOSOME EXPERIMENTS 38
6.7.5 SFV INOCULATION OF LIPOSOMES 39 6.8 ELECTRON MICROSCOPY 39 6.8.1
SAMPLE PREPARATION 39 6.8.1.1 FIDUCIAL GOLD MARKERS 39 6.8.1.2 NEGATIVE
STAIN 39 6.8.1.3 CRYO-NEGATIVE STAIN 40 6.8.1.4 PLUNGE-FREEZING 40 6.8.2
DATA ACQUISITION 40 6. 1 TABLE OF CONTENTS III 7.1.2 ENTRY INTO
SYNAPTOSOMES 48 7.1.2.1 STRUCTURAL DYNAMICS DURING HSV-1 ENTRY 49
7.1.2.2 CHANGE IN MEMBRANE CURVATURE DURING ENTRY 52 7.1.2.3 POST-FUSION
MORPHOLOGY OF VIRAL GLYCOPROTEINS 52 7.1.2.4 NECTIN-1 IS THE RECEPTOR
FOR HSV-1 ENTRY IN SYNAPTOSOMES 52 7.1.3 ENTRY INTO LIPOSOMES 53 7.1.4
PRE-AND POST-ENTRY HSV-1 CAPSID MORPHOLOGY 54 7.1.5 GLYCOPROTEIN
INTERMEDIATE STRUCTURES DURING HSV-1 ENTRY 55 7.1.5.1 CONNECTING
STRUCTURES BETWEEN HOST AND VIRUS MEMBRANE 55 7.1.5.2 ORIENTATION OF THE
VIRION DURING ENTRY 57 7.1.5.3 MODEL FOR FUSION MECHANISM 58 7.2
ANALYSIS OF HSV-1 ENTRY-ASSOCIATED GLYCOPROTEINS ON ISOLATED VIRIONS 60
7.2.1 OPTIMISATION AND CHARACTERISATION OF VIRUS PREPARATIONS 60 7.2.2
QUANTITATION OF ENTRY-ASSOCIATED VIRAL GLYCOPROTEINS 63 7.2.3 SCREENING
OF ANTIBODIES FOR BINDING TO THE NATIVE VIRION 67 7.2.4 GOLD-LABELLING
OF NATIVE ENTRY-ASSOCIATED GLYCOPROTEINS 67 7.3 STRUCTURES OF HSV
ENTRY-ASSOCIATED GLYCOPROTEINS 72 7.3.1 STRUCTURE OF GB730 BOUND TO
LIPOSOMES 72 7.3.1.1 THE GB ECTODOMAIN BINDS TO LIPOSOMES IN A DEFINED
ORIENTATION 73 7.3.1.2 CRYO-ET STRUCTURE OF GB730 BOUND TO LIPOSOMES 73
7.3.1.3 DIFFERENCE MAP OF THE X-RAY AND CRYO-ET GB73O STRUCTURE 74
7.3.1.4 MODEL FOR GB730 BINDING TO THE MEMBRANE 75 7.3.2 COFLOTATION
ASSAYS WITH GB730 AND GB730-Y179S 76 7.3.3 CRYO-EM OF GB773 77 7.3.4
MORPHOLOGY OF HSV-2 GH/GL 78 7.4 VISUALISATION OF MEMBRANE FUSION 79
7.4. |
| any_adam_object | 1 |
| author | Maurer, Ulrike Elisabeth |
| author_GND | (DE-588)139838287 |
| author_facet | Maurer, Ulrike Elisabeth |
| author_role | aut |
| author_sort | Maurer, Ulrike Elisabeth |
| author_variant | u e m ue uem |
| building | Verbundindex |
| bvnumber | BV035844904 |
| classification_tum | CHE 890d CHE 820d BIO 303d |
| ctrlnum | (OCoLC)643446951 (DE-599)DNB998026905 |
| dewey-full | 616.9041 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.9041 |
| dewey-search | 616.9041 |
| dewey-sort | 3616.9041 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Chemie Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-10-21T12:06:51Z |
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| isbn | 9783868532012 |
| language | English |
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| physical | VII, 111 S. Ill., graph. Darst. 210 mm x 148 mm, 201 gr. |
| publishDate | 2009 |
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| series2 | Biochemie |
| spelling | Maurer, Ulrike Elisabeth Verfasser (DE-588)139838287 aut Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses Ulrike Elisabeth Maurer 1. Aufl. München Verl.Dr. Hut 2009 VII, 111 S. Ill., graph. Darst. 210 mm x 148 mm, 201 gr. txt rdacontent n rdamedia nc rdacarrier Biochemie München, Techn. Univ., Diss., 2009 Herpes-simplex-Virus (DE-588)4137122-7 gnd rswk-swf Elektronenstrahltomografie (DE-588)4303135-3 gnd rswk-swf Glykoproteine (DE-588)4071905-4 gnd rswk-swf Membranfusion (DE-588)4210049-5 gnd rswk-swf Kryoelektronenmikroskopie (DE-588)4500745-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Herpes-simplex-Virus (DE-588)4137122-7 s Membranfusion (DE-588)4210049-5 s Glykoproteine (DE-588)4071905-4 s Kryoelektronenmikroskopie (DE-588)4500745-7 s Elektronenstrahltomografie (DE-588)4303135-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018703123&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Maurer, Ulrike Elisabeth Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses Herpes-simplex-Virus (DE-588)4137122-7 gnd Elektronenstrahltomografie (DE-588)4303135-3 gnd Glykoproteine (DE-588)4071905-4 gnd Membranfusion (DE-588)4210049-5 gnd Kryoelektronenmikroskopie (DE-588)4500745-7 gnd |
| subject_GND | (DE-588)4137122-7 (DE-588)4303135-3 (DE-588)4071905-4 (DE-588)4210049-5 (DE-588)4500745-7 (DE-588)4113937-9 |
| title | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |
| title_auth | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |
| title_exact_search | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |
| title_full | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses Ulrike Elisabeth Maurer |
| title_fullStr | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses Ulrike Elisabeth Maurer |
| title_full_unstemmed | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses Ulrike Elisabeth Maurer |
| title_short | Structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |
| title_sort | structural analysis of viral glycoproteins and membrane fusion during host entry of enveloped viruses |
| topic | Herpes-simplex-Virus (DE-588)4137122-7 gnd Elektronenstrahltomografie (DE-588)4303135-3 gnd Glykoproteine (DE-588)4071905-4 gnd Membranfusion (DE-588)4210049-5 gnd Kryoelektronenmikroskopie (DE-588)4500745-7 gnd |
| topic_facet | Herpes-simplex-Virus Elektronenstrahltomografie Glykoproteine Membranfusion Kryoelektronenmikroskopie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018703123&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT maurerulrikeelisabeth structuralanalysisofviralglycoproteinsandmembranefusionduringhostentryofenvelopedviruses |