Biomolecular EPR spectroscopy:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boca Raton [u.a.]
CRC Press
2009
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 249 S. Ill., graph. Darst. |
| ISBN: | 9781420059571 1420059572 |
Internformat
MARC
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| 100 | 1 | |a Hagen, Wilfred R. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Biomolecular EPR spectroscopy |c Wilfred Raymond Hagen |
| 264 | 1 | |a Boca Raton [u.a.] |b CRC Press |c 2009 | |
| 300 | |a XII, 249 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Electron paramagnetic resonance spectroscopy | |
| 650 | 4 | |a Biomolecules |x Analysis | |
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| 650 | 0 | 7 | |a Biomaterial |0 (DE-588)4267769-5 |2 gnd |9 rswk-swf |
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| 689 | 0 | |C b |5 DE-604 | |
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Datensatz im Suchindex
| _version_ | 1804140767097126912 |
|---|---|
| adam_text | Table
of Contents
Preface
.......................................................................................................................xi
Part
1
Basics
Chapter
1
Introduction
........................................................................................3
1.1
Overview of biomolecular EPR spectroscopy
...................................................3
1.2
How to use this book and associated software
.................................................4
1.3
A brief history of bioEPR
.................................................................................5
Chapter
2
The Spectrometer
................................................................................9
2.1
The concept of magnetic resonance
..................................................................9
2.2
The microwave frequency
...............................................................................12
2.3
Overview of the spectrometer
.........................................................................15
2.4
The resonator
..................................................................................................17
2.5
From source to detector
..................................................................................20
2.6
The magnet
.....................................................................................................22
2.7
Phase-sensitive detection
.................................................................................23
2.8
Tuning the spectrometer
.................................................................................25
2.9
Indicative budget considerations
.....................................................................27
Chapter
3
The Sample
.......................................................................................33
3.1
Sample tube and sample size
...........................................................................33
3.2
Freezing and thawing
......................................................................................36
3.3
Solid air problem
.............................................................................................39
3.4
Biological relevance of a frozen sample
.........................................................40
3.5
Sample preparation on the vacuum/gas manifold
...........................................43
3.6
Choice of reactant
...........................................................................................47
3.7
Gaseous substrates
..........................................................................................49
3.8
Liquid samples
................................................................................................50
3.9
Notes on safety
................................................................................................51
Chapter
4
Experimental Key Parameters
..........................................................53
4.1
Boltzmann and
Heisenberg
dictate optimal (P,T) pairs
..................................53
4.2
Homogeneous versus inhomogeneous lines
....................................................58
4.3
Spin multiplicity and its practical implications
..............................................61
VII
v¡¡¡
Contents
Chapter
5
Resonance Condition
........................................................................67
5.1
Main players in EPR theory: B, S, and 1
.........................................................67
5.2
Anisotropy
.......................................................................................................
I
5.3
Hyperfine interactions
.....................................................................................75
5.4
Second-order effects
.......................................................................................
78
5.5
Low-symmetry effects
....................................................................................80
5.6
Zero-field interactions
.....................................................................................82
5.7
Integer spins
....................................................................................................87
5.8
Interpretation of
g, A, D
..................................................................................89
Chapter
6
Analysis
............................................................................................95
6.1
Intensity
...........................................................................................................95
6.2
Quantification
..................................................................................................96
6.3
Walking the unit sphere
................................................................................100
6.4
Difference spectra
.........................................................................................103
Part
2
Theory
Chapter
7
Energy Matrices
..............................................................................109
7.1
Preamble to Part
2.........................................................................................109
7.2
Molecular Hamiltonian and spin Hamiltonian
.............................................112
7.3
Simple example:
S
= 1/2................................................................................115
7.4
Not-so-simple example:
S
= 3/2....................................................................119
7.5
Challenging example: integer spin
S
> 2.......................................................123
7.6
Compounded (or product) spin wavefunctions
..............................................131
Chapter
8
Biological Spin Hamiltonians
.........................................................135
8.1
Higher powers of spin operators
...................................................................135
8.2
Tensor noncolinearity
...................................................................................140
8.3
General EPR intensity expression
.................................................................141
8.4
Numerical implementation of diagonalization solutions
..............................145
8.5
A brief on perturbation theory
......................................................................147
Chapter
9
Conformational Distributions
.........................................................153
9.1
Classical models of anisotropic linewidth
.....................................................153
9.2
Statistical theory of g-strain
..........................................................................157
9.3
Special case of full correlation
......................................................................159
9.4
A (brojmolecular interpretation of g-strain
...................................................162
9.5
А
-strain and D-strain: coupling to other interactions
...................................164
Contents ix
Part
3
Specific Experiments
Chapter
10
Aqueous Solutions
.........................................................................169
10.1
Spin traps
.....................................................................................................169
10.2
Spin labels in
isotropie
media
.....................................................................171
10.3
Spin labels in anisotropic media
.................................................................177
10.4 Metalloproteins in
solution
.........................................................................179
Chapter
11
Interactions
.....................................................................................181
11.1
Dipole-dipole interactions
..........................................................................181
11.2
Dipolar interaction in
multicenter
proteins
.................................................184
11.3
Exchange interactions
..................................................................................188
11.4
Spin ladders
.................................................................................................193
11.5
Valence isomers
...........................................................................................196
11.6
Superparamagnetism
...................................................................................197
Chapter
12
High Spins Revisited
......................................................................199
12.1
Rhombograms for
S
= 7/2
and
S
= 9/2........................................................199
12.2
D-strain modeled as a rhombicity distribution
...........................................204
12.3
Population of half-integer spin
multiplets
...................................................205
12.4
Intermediate-field case for
S
= 5/2..............................................................207
12.5
Analytical lineshapes for integer spins
.......................................................208
Chapter
13
Black Box Experiments
.................................................................213
13.1
EPR-monitored binding experiments
..........................................................214
13.2
EPR monitoring of
redox
states
..................................................................215
13.3
EPR monitored kinetics
..............................................................................221
13.4
EPR of whole cells and organelles
..............................................................222
Chapter
14
Strategic Considerations
................................................................225
14.1
Bio-integrated bioEPR
.................................................................................225
14.2
To be advanced or not to be advanced
........................................................226
14.3
Friday afternoon experiment
.......................................................................228
References
.............................................................................................................231
Index
.....................................................................................................................241
|
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| dewey-sort | 3538 3364 |
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| discipline | Chemie / Pharmazie Physik Biologie |
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| genre_facet | Einführung |
| id | DE-604.BV035814195 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:05:12Z |
| institution | BVB |
| isbn | 9781420059571 1420059572 |
| language | English |
| lccn | 2008048857 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018673062 |
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| physical | XII, 249 S. Ill., graph. Darst. |
| publishDate | 2009 |
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| publisher | CRC Press |
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| spelling | Hagen, Wilfred R. Verfasser aut Biomolecular EPR spectroscopy Wilfred Raymond Hagen Boca Raton [u.a.] CRC Press 2009 XII, 249 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Electron paramagnetic resonance spectroscopy Biomolecules Analysis Elektronenspinresonanzspektroskopie (DE-588)4151888-3 gnd rswk-swf Biomaterial (DE-588)4267769-5 gnd rswk-swf (DE-588)4151278-9 Einführung gnd-content Elektronenspinresonanzspektroskopie (DE-588)4151888-3 s Biomaterial (DE-588)4267769-5 s b DE-604 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018673062&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hagen, Wilfred R. Biomolecular EPR spectroscopy Electron paramagnetic resonance spectroscopy Biomolecules Analysis Elektronenspinresonanzspektroskopie (DE-588)4151888-3 gnd Biomaterial (DE-588)4267769-5 gnd |
| subject_GND | (DE-588)4151888-3 (DE-588)4267769-5 (DE-588)4151278-9 |
| title | Biomolecular EPR spectroscopy |
| title_auth | Biomolecular EPR spectroscopy |
| title_exact_search | Biomolecular EPR spectroscopy |
| title_full | Biomolecular EPR spectroscopy Wilfred Raymond Hagen |
| title_fullStr | Biomolecular EPR spectroscopy Wilfred Raymond Hagen |
| title_full_unstemmed | Biomolecular EPR spectroscopy Wilfred Raymond Hagen |
| title_short | Biomolecular EPR spectroscopy |
| title_sort | biomolecular epr spectroscopy |
| topic | Electron paramagnetic resonance spectroscopy Biomolecules Analysis Elektronenspinresonanzspektroskopie (DE-588)4151888-3 gnd Biomaterial (DE-588)4267769-5 gnd |
| topic_facet | Electron paramagnetic resonance spectroscopy Biomolecules Analysis Elektronenspinresonanzspektroskopie Biomaterial Einführung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018673062&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hagenwilfredr biomoleculareprspectroscopy |