Non-natural amino acids:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, AP
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
462 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIII, 264 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123743107 |
Internformat
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| 245 | 1 | 0 | |a Non-natural amino acids |c ed. by Tom W. Muir ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, AP |c 2009 | |
| 300 | |a XLIII, 264 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 462 | |
| 650 | 4 | |a Amino Acids |x chemistry | |
| 650 | 4 | |a Amino acids | |
| 650 | 4 | |a Protein Engineering | |
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| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018637476&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-018637476 | ||
Datensatz im Suchindex
| _version_ | 1804140711317078016 |
|---|---|
| adam_text | Contents
Contributors
ix
Preface
xiii
Volumes in Series
xvii
1.
Protein Phosphorylation by Semisynthesis:
From Paper to Practice
1
Lawrence
M. Szewczuk, Mary Katherine Tarrant,
and Philip A. Cole
1.
Overview of Protein Phosphorylation
2
2.
Investigating Protein Phosphorylation with Phosphomimetics
3
3.
Phosphonate Analogues and Protein Semisynthesis
5
4.
Methods
6
5.
Practical Uses of Semisynthetic Phosphoproteins
14
6.
Future of Protein Semisynthesis in Signaling
19
Acknowledgments
21
References
21
2.
Protein Engineering with the Traceless Staudinger Ligation
25
Annie Tarn and Ronald T. Raines
1.
Introduction
26
2.
Traceless Staudinger Ligation
26
3.
Choice of Coupling Reagent
28
4.
Preparation of the Azido Fragment
33
5.
Preparation of the Phosphinothioester Fragment
35
6.
Protein Assembly by Orthogonal Chemical Ligations
40
7.
Prospectus
41
Acknowledgments
43
References
43
3.
Replacement of Y730 and Y731 in the
al Subunit
of
Escherichia
coli Ribonucleotide
Reducíase
with 3-Aminotyrosine
using an Evolved Suppressor tRNA/tRNA-Synthetase Pair
45
Mohammad R. Seyedsayamdost and JoAnne
Stubbe
1.
Introduction
47
vi
Contents
2.
Site-Specific Insertion of Unnatural
Amino
Acids using the
Suppressor tRNA/RS Method
49
3.
NH2Y,
a Y
Analogue for Investigating Enzymatic PCET Reactions
51
4.
Directed Evolution of NHjY-RS in
E. coli
53
5.
Examination of the Fidelity and Specificity of NH2Y
Incorporation in a Protein Expressed in
E. coli
55
6.
Generation of
Yr30NH2Y-a2
and YjgiNHzY-oa
57
7.
Characterization of NHzY-^s
65
8.
Summary
71
Acknowledgments
71
References
71
4.
Semisynthesis of Proteins Using Split Inteins
77
Christina
Ludwig, Dirk Schwarzer,
Joachim Zettler, Daniel
Garbe,
Petra Janning, Claus
Czeslik, and
Henning
D. Mootz
1.
Introduction
78
2.
Protein Splicing in
cis
and in trans is Performed by Inteins
81
3.
Design of Split Inteins and Considerations on the Intein Insertion Site
82
4.
Materials and Methods
87
5.
Summary and Conclusion
92
Acknowledgments
93
References
93
5.
Expressed Protein Ligation for
Metalloprotein
Design
and Engineering
97
Kevin M. Clark, Wilfred A. van
der Donk,
and Yi
Lu
1.
Introduction
98
2.
Methods of Selenocysteine Incorporation
101
3.
Incorporation of Selenocysteine into the Type
1
Copper
Site of Azurin
102
4.
Tuning the Type
1
Copper Reduction Potential Using
Isostructural Methionine
Analogues
109
5.
Conclusion 111
Acknowledgments
112
References
113
6.
Using Expressed Protein Ligation to Probe the
Substrate Specificity of Lantibiotic Synthetases
117
Xingang Zhang and Wilfred A. van
der Donk
1.
Introduction
118
Contents
vii
2.
Use of Expressed Protein Ligation to Prepare Substrate Analogues
120
3.
Conclusion
132
Acknowledgments
133
References
133
7.
Semisynthesis of
К
Channels
135
Alexander
6.
Komárov,
Kellie
M.
Linn, Jordan
J. Devereaux,
and Francis
I.
Valiyaveetil
1.
Introduction
136
2.
Experimental Protocols
139
3.
Application of Semisynthesis
in Investigating the
Selectivity Filter of the K+ Channels
147
4.
Summary
148
Acknowledgment
149
References
149
8.
Segmental
Isotopie
Labeling of Proteins for Nuclear
Magnetic Resonance
151
Dongsheng Liu, Rong Xu, and David Cowburn
1.
Introduction
152
2.
Segmentai Isotopie
Labeling using Expressed Protein Ligation
153
3.
Segmental
Labeling using Protein Trans-Splicing
159
4.
Multiple Segment Assembly
160
5.
Segmental
Labeling of
C-Terminal
SRC Kinase(Csk)
160
Acknowledgments
171
References
171
9.
Semisynthesis of Membrane-Attached Prion Proteins
177
Nam Ky
Chu
and Christian
F. W.
Becker
1.
Introduction
178
2.
Chemical Synthesis of Membrane Anchors
179
3.
Semisynthesis of rPrP^1 1 by Expressed Protein Ligation
180
4.
Semisynthesis of
rPrPPalm
by Protein Trans-Splicing
185
5.
Liposome
Attachment and Aggregation of
rPrPPalm
and rPrPGPI
188
6.
Conclusion
190
References
190
viii Contents
10.
Use of Intein-Mediated Protein Ligation Strategies
for the Fabrication of Functional Protein Arrays
195
Souvik Chattopadhaya, Farhana B. Abu
Bakar,
and Shao Q. Yao
1.
Introduction
196
2.
Intein-Mediated Protein Ligation Strategies
197
3.
In Vitro, In Viva and Cell Free Strategies for Protein
Biotinylation at the
C-Terminal
203
4.
Methods
207
5.
Concluding Remarks
219
References
220
11.
Semisynthesis of Ubiquitylated Proteins
225
Robert K. McGinty, Champak Chatterjee, and Tom W.
Muir
1.
Introduction
226
2.
Semisynthesis of Ubiquitylated Histone H2B
228
3.
Methods
230
4.
Synthesis of Photodeavable Ligation Auxiliary
231
5.
Peptide
Synthesis
234
6.
Generation of
Recombinant
Protein a-Thioesters
235
7.
Expressed Protein Ligation
237
8.
Generation of Ubiquitylated Mononucleosomes
239
9.
Conclusions
242
Acknowledgments
242
References
242
Author Index
245
Subject Index
259
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| id | DE-604.BV035777905 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:04:19Z |
| institution | BVB |
| isbn | 9780123743107 |
| language | English |
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| physical | XLIII, 264 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Non-natural amino acids ed. by Tom W. Muir ... 1. ed. Amsterdam [u.a.] Elsevier, AP 2009 XLIII, 264 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 462 Amino Acids chemistry Amino acids Protein Engineering Proteins Proteins chemistry Reaktionsmechanismus (DE-588)4177123-0 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf Aminosäuren (DE-588)4142205-3 gnd rswk-swf Teilsynthese (DE-588)4508944-9 gnd rswk-swf Proteindesign (DE-588)4346801-9 gnd rswk-swf Aminosäuren (DE-588)4142205-3 s Enzym (DE-588)4014988-2 s Reaktionsmechanismus (DE-588)4177123-0 s DE-604 Teilsynthese (DE-588)4508944-9 s Proteindesign (DE-588)4346801-9 s b DE-604 Muir, Tom W. Sonstige oth Methods in enzymology 462 (DE-604)BV000000938 462 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018637476&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Non-natural amino acids Methods in enzymology Amino Acids chemistry Amino acids Protein Engineering Proteins Proteins chemistry Reaktionsmechanismus (DE-588)4177123-0 gnd Enzym (DE-588)4014988-2 gnd Aminosäuren (DE-588)4142205-3 gnd Teilsynthese (DE-588)4508944-9 gnd Proteindesign (DE-588)4346801-9 gnd |
| subject_GND | (DE-588)4177123-0 (DE-588)4014988-2 (DE-588)4142205-3 (DE-588)4508944-9 (DE-588)4346801-9 |
| title | Non-natural amino acids |
| title_auth | Non-natural amino acids |
| title_exact_search | Non-natural amino acids |
| title_full | Non-natural amino acids ed. by Tom W. Muir ... |
| title_fullStr | Non-natural amino acids ed. by Tom W. Muir ... |
| title_full_unstemmed | Non-natural amino acids ed. by Tom W. Muir ... |
| title_short | Non-natural amino acids |
| title_sort | non natural amino acids |
| topic | Amino Acids chemistry Amino acids Protein Engineering Proteins Proteins chemistry Reaktionsmechanismus (DE-588)4177123-0 gnd Enzym (DE-588)4014988-2 gnd Aminosäuren (DE-588)4142205-3 gnd Teilsynthese (DE-588)4508944-9 gnd Proteindesign (DE-588)4346801-9 gnd |
| topic_facet | Amino Acids chemistry Amino acids Protein Engineering Proteins Proteins chemistry Reaktionsmechanismus Enzym Aminosäuren Teilsynthese Proteindesign |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018637476&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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