Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:19-105870 Inhaltsverzeichnis |
| Beschreibung: | 103 S. Ill., graph. Darst. |
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MARC
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| 245 | 1 | 0 | |a Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |c von Katharina Engelke |
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow
Autor: Engelke, Katharina
Jahr: 2009
Index of Content
LIST OF FIGURES..............................................................................................1-5
LIST OF TABLES...............................................................................................1-7
ABBREVIATIONS...............................................................................................1-8
1 INTRODUCTION..........................................................................................1-9
1.1 Bone Marrow.......................................................................................1-9
1.1.1 Bone Manow Function...................................................................1-9
1.1.2 Bone Marrow Configuration.........................................................1-10
1.1.3 Previous Intravital Microscopy Studies in the Bone Marrow........1-12
1.2 Multiphoton Microscopy..................................................................1-15
1.2.1 History, Theory and Physics of Multiphoton Microscopy..............1-15
1.2.2 Previous Multiphoton Microscopy Studies...................................1-21
2 AIMS OF THE STUDY...............................................................................2-24
3 MATERIALS AND METHODS...................................................................3-26
3.1 Experimental Design.........................................................................3-26
3.1.1 Place of the Study........................................................................3-26
3.1.2 Components.................................................................................3-26
3.1.3 Preparation of the Animal............................................................3-35
3.1.4 Intravital Microscopy Experiments...............................................3-37
3.2 Image Processing and Analysis.........................................................3-44
3.2.1 Quantification of Bone Marrow.....................................................3-44
3.2.2 Adoptive Transfer Experiments....................................................3-50
3.2.3 Vessel Diameter Measurements..................................................3-51
3.2.4 3D Reconstruction from Processed Image Stacks.......................3-52
3.3 Statistics...........................................................................................3-52
3.3.1 Bone Manow Quantification.........................................................3-52
3.3.2 Adoptive Transfer Experiments....................................................3-52
4 RESULTS..................................................................................................4-54
4.1 Anatomy and Morphology of the Bone Marrow..............................4-54
4.2 Quantification of Bone Marrow Compartments.............................4-56
4.3 Adoptive Transfer Experiments.......................................................4-61
5 DISCUSSION.............................................................................................5-67
5.1 Multiphoton Imaging in the Bone Marrow Organ............................5-67
5.2 Experimental Design of the Study...................................................5-74
5.3 Image Processing and Analysis.........................................................5-79
5.4 Spatial Relationships in the Bone Marrow......................................5-82
5.5 Subcompartmentalization of the Bone Marrow..............................5-84
6 SUMMARY.................................................................................................6-87
7 ZUSAMMENFASSUNG.............................................................................7-88
8 REFERENCES...........................................................................................8-90
9 DANKSAGUNG.......................................................................................9-102
10 CURRICULUM VITAE........................................................................10-103
1-5
List of Figures
Figure 1. Organization of BM microvasculature in murine skull BM..................1-14
Figure 2. CLSM in scattering media..................................................................1-16
Figure 3. Principle of multiphoton excitation compared to confocal excitation... 1-18
Figure 4. MPM in scattering media....................................................................1-19
Figure 5. Phenotype of TCm...............................................................................3-30
Figure 6. Phenotype of total splenocytes..........................................................3-31
Figure 7. MACS purification of naive B-cells by negative selection...................3-33
Figure 8. Phenotype of enriched naive B-cells..................................................3-34
Figure 9. Animal preparation and immobilization technique of the mouse........3-36
Figure 10. Configuration of the MPM system....................................................3-38
Figure 11. Image acquisition during the experiment..........................................3-41
Figure 12. Montage of the BM region investigated during one experiment.....3-42
Figure 13. Additional Image Acquisition in Adoptive Transfer Experiments......3-44
Figure 14. Image processing technique in Adobe Photoshop...........................3-47
Figure 15. Comparison of a raw data section with its corresponding processed
section........................................................................................................3-48
Figure 16. Measurement performance by VoxBIast..........................................3-49
Figure 17. Image analysis in adoptive transfer experiments.............................3-51
1-6
Figure 18. Arrangement of microvascular segments in the frontoparietal region of
skull BM......................................................................................................4-55
Figure 19. IV at different depth below skull surface...........................................4-56
Figure 20. Spatial relationships of BM compartments in different microvascular
segments....................................................................................................4-57
Figure 21. 3D reconstruction of an IV................................................................4-61
Figure 22. Ton home to the BM compartment...................................................4-62
Figure 23. Analysis of TCm homing.....................................................................4-64
Figure 24. Analysis of naive B-cell homing.......................................................4-66
Figure 25. Comparison of MPM and CLSM in XZ images of a CV....................5-69
Figure 26. Comparison MPM and CLSM in XY images of a CV........................5-69
Figure 27. Penetration depth in MPM................................................................5-71
Figure 28. Fluorescence distribution in a BM cavity..........................................5-77
1-7
List of Tables
Table 1. Diameters and ratios of intravascular to extravascular space for different
microvascular segments.............................................................................4-58
Table 2. Dimensions of BM microvessels by Mazo et. al..................................4-58
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| spelling | Eder, Katharina 1979- Verfasser (DE-588)139396098 aut Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow von Katharina Engelke 2009 103 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2009 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-105870 http://edoc.ub.uni-muenchen.de/10587/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-105870 Resolving-System HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018618690&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Eder, Katharina 1979- Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| subject_GND | (DE-588)4113937-9 |
| title | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| title_auth | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| title_exact_search | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| title_full | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow von Katharina Engelke |
| title_fullStr | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow von Katharina Engelke |
| title_full_unstemmed | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow von Katharina Engelke |
| title_short | Intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| title_sort | intravital multiphoton microscopy analysis of spatial relationships in murine skull bone marrow |
| topic_facet | Hochschulschrift |
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