Membrane protein crystallization:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Current topics in membranes
63 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 305 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123749871 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
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| 003 | DE-604 | ||
| 005 | 20090904 | ||
| 007 | t | ||
| 008 | 090826s2009 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123749871 |9 978-0-12-374987-1 | ||
| 035 | |a (OCoLC)303020781 | ||
| 035 | |a (DE-599)BVBBV035694649 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
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| 084 | |a WE 5000 |0 (DE-625)148296: |2 rvk | ||
| 245 | 1 | 0 | |a Membrane protein crystallization |c ed. by Larry DeLucas |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2009 | |
| 300 | |a XX, 305 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Current topics in membranes |v 63 | |
| 650 | 4 | |a Crystallization | |
| 650 | 4 | |a Membrane Proteins |x physiology | |
| 650 | 4 | |a Membrane proteins | |
| 650 | 0 | 7 | |a Kristallisation |0 (DE-588)4033215-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Membranproteine |0 (DE-588)4130026-9 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Membranproteine |0 (DE-588)4130026-9 |D s |
| 689 | 0 | 1 | |a Kristallisation |0 (DE-588)4033215-9 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a DeLucas, Larry |e Sonstige |4 oth | |
| 830 | 0 | |a Current topics in membranes |v 63 |w (DE-604)BV036839511 |9 63 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017748669&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017748669 | ||
Datensatz im Suchindex
| _version_ | 1804139407733686272 |
|---|---|
| adam_text | Contents
Contributors
xi
Preface
xv
Previous Volumes in Series
xvii
Introduction
1
CHAPTER
1
Introduction to the Crystallization of
Biological Macromolecules
Alex McPherson
I. Overview
5
II. Introduction
6
III. The Requirement for
Supersaturation
6
References
22
CHAPTER
2
In Vitro Synthesis of Posttranslationally
Modified Membrane Proteins
Stefan
Kubiek,
Michael
Gerrits,
Helmut
Merk,
Wolfgang
Stiege,
and
Volker
A. Erdtnann
I. Overview
25
II. Introduction
26
III. Materials and Methods
29
IV. Results
30
V. Conclusions
46
References
47
CHAPTER
3
Harnessing Photosynthetic Bacteria for
Membrane Protein Production
Deborah K. Hanson, Donna L. Mielke, and Philip D. Laible
I. Overview
52
II. Introduction
52
III. Foreign Gene Expression in
Rhodobacter sphaeroides
57
vii
Contents
IV.
Membrane Protein
Preparations for Structural
and Functional Studies
68
V. Practical Aspects of Heterologous Protein
Expression in Rhodobacter
73
VI. Concluding Statements
77
References
78
CHAPTER
4
Monoacylglycerols: The Workhorse Lipids for
Crystallizing Membrane Proteins in Mesophases
M. Caffrey, J. Lyons, T. Smyth, and D. J. Hart
I. Overview
83
II. Introduction
84
III. In
Meso Crystallogenesis
in Molecular Detail
IV. Mesophase Behavior: From Lamellar
to Cubic and Sponge Phases
87
V. A Central Role for
Lipid
91
VI.
Lipid
Synthesis and Purification
95
VII.
Conclusions
105
References
106
86
CHAPTER
5
Practical Aspects of Membrane Proteins
Crystallization in Bicelles
Salem Faham, Rachna Ujwal, Jeff Abramson, and James U. Bowie
I. Overview
109
II. Introduction
110
III. Bicelles
112
IV. Phase Behavior of Bicelles
113
V. Examples of Membrane Proteins
Crystallized in Bicelles
114
VI. Crystal Packing
118
VII.
Practical Information
118
VIII.
Conclusion
122
References
123
CHAPTER
6
Membrane Protein Crystallization: Approaching
the Problem and Understanding the Solutions
Mads Gabrieken, Alastair T. Gardiner.
Petra Fromme,
and Richard J. Cogdell
I. Overview
128
II. Introduction
128
Contents
III. The Basic Crystallization Process
128
IV. Vapor Diffusion
131
V. Microdialysis
133
VI. Microfluidics
134
VII.
Counter-Diffusion
134
VIII.
Novel Methods using Lipids in Crystallization
135
IX. How to obtain Crystals from a Membrane
Protein of Choice
137
X. Optimization of Initial Hits
139
XI. Seeding
140
XII.
Is it Possible to Preselect Crystallization
Conditions That are Inherently More Favorable
for Crystallization?
144
XIII.
Conclusions
145
References
146
CHAPTER
7
Tools to Enhance Membrane Protein Crystallization
W. William Wilson, Gregg Whited, Robert W. Payne, Charles Henry,
David H. Johnson, and Larry
DeLucas
I. Overview
152
II. Introduction
152
III. Protein-Protein Interactions
and Crystallization
153
IV. Self-Interaction Chromatography
156
V. Validation Studies: SIC Accurately
Quantifies
ß 158
References
175
CHAPTER
8
Advances in Microfluidic Membrane Protein
Crystallization Techniques
Cory
Geräts
and Peter
Notiert
I. Overview
179
II. Membrane Protein Crystallization
180
III. Microfluidics
180
IV. Crystallization by FID in Polydimethylsiloxane
(PDMS) Devices
182
V. Membrane Protein Crystallization in Gradients
Established in
Microchannels 184
VI. Plug-Based Membrane Protein Crystallization
in Microcapillaries
185
VII.
Conclusion
187
References
189
Contents
CHAPTER
9
Crystallization of Photosynthetic
Membrane Proteins
Petra Fromme
and
Ingo Grotjohann
I. Overview
192
II. Overview of Crystallization of Photosynthetic
Membrane Proteins
192
III. Biological Parameters that Influence
Crystallization
196
IV. Physical-Chemical Parameters that Influence
Crystallization
202
V. Crystallization Techniques
209
VI. Determination of Phase Diagrams
216
VII.
Seeding Techniques
221
References
224
CHAPTER
10
A Practical Guide to
Х
-Ray Crystallography
of /3-BarreI Membrane Proteins: Expression,
Purification, Detergent Selection, and
Crystallization
Mikio Tanabe and Tina M.
¡versori
I. Overview
230
II. Introduction
230
III. Expression and Purification
232
IV. Preliminary Crystallization and
Diffraction-Based Optimization
252
V. Materials and Methods
262
References
263
CHAPTER
í í
Bacterial Membrane Proteins: The New
Soluble Proteins?
Hubing Lou,
Konstantinos
Beis,
and James H. Naismith
I. Overview
269
II. Structures of Omps
270
III.
Recombinant
Production of Omps
284
IV.
Recombinant
Bacterial Inner Membrane Proteins
and Eukaryotic Membrane Proteins
286
V. Crystallization of Membrane Proteins
287
VI. New Strategies for Eukaryotic Proteins
from the Study of GPCR
288
VII.
Summary
291
References
292
Index
299
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV035694649 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:43:36Z |
| institution | BVB |
| isbn | 9780123749871 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017748669 |
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| open_access_boolean | |
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| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | XX, 305 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Current topics in membranes |
| series2 | Current topics in membranes |
| spelling | Membrane protein crystallization ed. by Larry DeLucas 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2009 XX, 305 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Current topics in membranes 63 Crystallization Membrane Proteins physiology Membrane proteins Kristallisation (DE-588)4033215-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Membranproteine (DE-588)4130026-9 s Kristallisation (DE-588)4033215-9 s b DE-604 DeLucas, Larry Sonstige oth Current topics in membranes 63 (DE-604)BV036839511 63 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017748669&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Membrane protein crystallization Current topics in membranes Crystallization Membrane Proteins physiology Membrane proteins Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4033215-9 (DE-588)4130026-9 (DE-588)4143413-4 |
| title | Membrane protein crystallization |
| title_auth | Membrane protein crystallization |
| title_exact_search | Membrane protein crystallization |
| title_full | Membrane protein crystallization ed. by Larry DeLucas |
| title_fullStr | Membrane protein crystallization ed. by Larry DeLucas |
| title_full_unstemmed | Membrane protein crystallization ed. by Larry DeLucas |
| title_short | Membrane protein crystallization |
| title_sort | membrane protein crystallization |
| topic | Crystallization Membrane Proteins physiology Membrane proteins Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Crystallization Membrane Proteins physiology Membrane proteins Kristallisation Membranproteine Aufsatzsammlung |
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