Chemokines: A
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
460 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIX, 459 S., [9] Bl. Ill., graph. Darst. |
| ISBN: | 9780123749086 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV035645139 | ||
| 003 | DE-604 | ||
| 005 | 20110803 | ||
| 007 | t | ||
| 008 | 090724s2009 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123749086 |9 978-0-12-374908-6 | ||
| 035 | |a (OCoLC)642998734 | ||
| 035 | |a (DE-599)BVBBV035645139 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-20 |a DE-91G |a DE-19 |a DE-12 | ||
| 245 | 1 | 0 | |a Chemokines |n A |c ed. by Tracy M. Handel ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2009 | |
| 300 | |a XLIX, 459 S., [9] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 460 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Handel, Tracy M. |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV035645025 |g 1 |
| 830 | 0 | |a Methods in enzymology |v 460 |w (DE-604)BV000000938 |9 460 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017699863&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017699863 | ||
Datensatz im Suchindex
| _version_ | 1804139328166690816 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xxi
Volumes in Series
xxiii
Section I. Chemokines and Receptors in Disease
1
1.
Chemokines in Human Breast Tumor Cells: Modifying Their
Expression Levels and Determining Their Effects on the
Malignancy Phenotype
3
Gali
Soria, Tsipi Meshel,
and Adit Ben-Baruch
1.
Introduction
4
2.
Modifying Chemokine Expression in Breast Tumor Cells
5
3.
Establishment of Primary Local Breast Tumors and
Pulmonary
Métastases
9
Acknowledgments
15
References
15
2.
CCR5 Pharmacology Methodologies and Associated Applications
17
Roy Mansfield, Sarah Able, Paul Griffin, Becky Irvine, Ian James,
Malcolm Macartney, Ken Miller, James Mills, Carolyn Napier,
lva Navrátilova,
Manos Perros,
Graham Rickett,
Harriet Root,
Elna
van
der Ryst, Mike Westby, and Patrick
Dorr
1.
Introduction
18
2.
CCR5 Signaling Assays and Application to Quantify and
Characterize Ligand-Dependent Agonism, Antagonism,
and Inverse Agonism
19
3.
CCRS-Associated Ligand-Binding Assays
33
4.
Surrogate In Vitro Antiviral Assays
38
5.
CCR5 Site-Directed Mutagenesis and Ligand Docking Studies
43
6.
Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice
48
References
52
Contents
3. CXCR4
and Mobilization of Hematopoietic Precursors
Michael P. Rettig, Pablo Ramirez,
Bruno Nervi,
and John F. DiPersio
58
1.
Introduction
2.
HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis
3.
Donor Selection for HSPC Mobilization 64
4.
Flow Cytometric Enumeration of Mobilized HSPCs 65
5.
Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor
66
6.
Flow Cytometric Analysis of CXCR4 Expression on
Human CD34+ Subsets
7.
Functional Characterization of Mobilized HSPCs
73
8.
Concluding Remarks
80
Acknowledgment
80
References
80
4.
Double-Label Nonradioactive In Situ Hybridization for the
Analysis of Chemokine Receptor Expression in the Central
Nervous System
91
Meizhang Li and Richard M. Ransohoff
1.
Introduction
92
2.
Basic Protocol for ISH (Using Digoxygenin-Labeled Probe)
93
3.
Comments
101
References
102
5.
Expression of Chemokines and Chemokine Receptors
in Human Colon Cancer
105
Marco Erreni, Paolo
Bianchi, Luigi Laghi,
Massimiliano
Mirólo,
Marco
Fabbri,
Massimo
Locati,
Alberto
Mantovani,
and
Paola Allavena
1.
Introduction 106
2.
Materials and Methods 108
3.
Results 110
4.
Discussion 117
Acknowledgments 119
References
Section II. Chemokine Related Proteins from Pathogens
123
6.
Kapósig
Sarcoma Virally Encoded, G-Protein-Coupled Receptor·
A Paradigm for Paracrine Transformation 125
Daniel Martin and
).
Silvio Gutkind
1.
Introduction
2.
Cloning of vGPCR 127
128
Contents
vii
3.
Assaying vGPCR Transforming Activity In Vitro
130
4.
vGPCR Transforming Activity In Vivo Using Xenograft Systems
130
5.
In Vivo Targeted Infection Using the TVA-RCAS System
131
6.
vGPCR-lnduced Paracrine Transformation
134
7.
Characterization of vGPCR-lnduced Molecular Signaling
135
8.
Evaluation of Activation of Second-Messenger-Generating Systems
136
9.
Activation of Signal-Transducing Protein Kinases and Small GTPases
137
10. Akt Kinase
Assay
137
11.
vGPCR Stimulated Activation of Rad-Pulldown Assays
138
12.
Western Blotting Using Phospho-Specific Antibodies
140
13.
Activation of Transcription Factors
141
14.
Global Changes in Gene Expression: Microarray Analysis
141
15.
NFkB Luciferase Assays
143
16.
NFkB Binding Assays
144
17.
Nuclear
Translocation
of NFkB
146
Acknowledgments
147
References
147
7.
Pharmacological and Biochemical Characterization of Human
Cytomegalovirus-Encoded
G
Protein-Coupled Receptors
151
David
Maussang,
Henry F. Vischer, Andreas
Schreiber, Detlef
Michel,
and
Martine
J.
Smit
1.
Introduction
152
2.
Virally Encoded GPCR Engineering
154
3.
vGPCR Expression, Trafficking, and Radioligand Binding
156
4.
vGPCR-lnduced Signal Transduction
159
5.
vGPCR-lnduced Oncogenesis
162
6.
Generation of
Recombinant HCMV
Strains by Markerless
Bacterial Artificial Chromosome Mutagenesis
165
7.
Conclusions
167
Acknowledgments
167
References
167
8.
Identification and Characterization of Virus-Encoded
Chemokine Binding Proteins
173
Antonio Alcami and
Abel Viejo-Borbolla
1.
Introduction
174
2.
Methods for Studying Chemokine-Binding Proteins
175
Acknowledgments
189
References
189
Contents
9. The Chemokine-Binding Protein M3
as a Tool to Understand the
Chemokine Network In Vivo 193
Sergio A. Lira,
Abel Viejo-Borbolla,
Limin Shang, and Andrea P. Martin
193
1.
Introduction
2.
Generation of Transgenic Mice Expressing M3
in Insulin-Producing
β
Cells 195
3.
M3 Expression in Islets of
Langerhans
Blocks CCL2-, CCL21-,
and CXCLO-lnduced Migration of Cells to Islets
198
4.
M3 Expression in
β
Cells Blocks Cellular Infiltration and
Prevents Diabetes Development
199
5.
Generation of a Conditional Transgenic System for Expression of M3
202
6.
Concluding Remarks
204
References
205
10.
M-T7: Measuring Chemokine-Modulating Activity
209
Mee Y.
Bartee, Erbin
Dai, Liying Liu, Ganesh Munuswamy-Ramanujam,
Colin Macaulay, Dana Mclvor, Grant McFadden, and Alexandra R. Lucas
1.
Introduction
210
2.
Protein Expression
213
3.
Quantifying the Effects of M-T7 In Vitro and Ex Vivo
216
4.
Quantifying the Effects of M-T7 on Vascular Inflammatory
Responses in Rodent Vascular Transplant Models
220
5.
Preclinical
Toxicity
Testing
225
References
227
Section III. Atypical and Novel Chemoattractants
and Receptors
229
11.
Role of the Chemokine Scavenger Receptor D6 in Balancing
Inflammation and Immune Activation
231
Elena M. Borroni,
Chiara Buracchi, Benedetta Savino, Fabio Pasqualini,
Remo
С.
Russo,
Manuela Nebuloni, Raffaella Bonecchi, Alberto
Mantovani,
and
Massimo Locati
1.
Introduction
2.
Methods
3.
Results 232
4.
Discussion 235
References 236
241
Contents
¡χ
12. Structure-Function
Dissection
of D6,
an
Atypical
Scavenger Receptor
245
Robert
J.
В.
Nibbs, Pauline McLean, Clare McCulloch, Alan Riboldi-Tunnicliffe,
Emma Blair, Yanshi Zhu, Neil Isaacs, and Gerard J. Graham
1.
Introduction
246
Acknowledgments
260
References
260
13.
Modeling Small Molecule-Compound Binding to
G-Protein-Coupled Receptors
263
Nagarajan Vaidehi, James E. Pease, and Richard Horuk
1.
Introduction
264
2.
Similarity and Differences in the Crystal Structures of Class
-А
GPCRs
Solved to Date
265
3.
GPCR Modeling Methods
266
4.
Computational Methods for Receptor Flexibility and Ligand-lnduced
Conformationat Changes in GPCRs
271
5.
Validation of GPCR-Ligand Models
274
6.
Conclusions
284
References
286
14.
Elucidation of Chemerin and Chemokine-Like Receptor-
1
Function
in Adipocytes by Adenoviral-Mediated shRNA Knockdown of
Gene Expression
289
Kerry B.
Góralski
and Christopher J. Sinai
1.
Introduction
290
2.
The 3T3-L1 Cell Model for Adipogenesis and Adipocyte Metabolism
292
3.
RNA
Interference
293
4.
Methods for Adenoviral shRNA Knockdown of Chemerin
and CMKLR1 in 3T3-L1 Cells
297
5.
Concluding Remarks
309
Acknowledgments
309
References
309
Section IV. Chemokine Signaling
313
15.
Characterization of Chemokine Receptor CXCR2
Interacting Proteins Using a Proteomics Approach to
Define
the CXCR2 Chemosynapse
315
Dayanidhi Raman, Nicole F. Neel, liqing
Sai,
Raymond
L Mernaugh,
Amy-Joan L. Ham, and Ann J. Richmond
Contents
316
1.
Introduction
_.—- 490
2.
Validation
of the Interaction of Novel Proteins with CXCR2
3.
Mutational Analysis of Residues at Interactive Interface
of CXCR2 and CXCItt-Binding Proteins 3
4.
Radioactive Phosphorylation of CXCR2 Interacting Proteins
326
5.
Chemotaxis Assay
6.
Conclusions
Acknowledgments
References
16.
Phosphoproteomic Analysis of Chemokine Signaling Networks
Morgan O Hayre, Catherine L.
Salanga,
Pieter C.
Dorrestein,
and Tracy
M.
Handel
1.
Introduction 332
2.
Methods 334
3.
Summary 343
Acknowledgments 3/*4
References 3^5
7.
Monitoring NF-kB Mediated Chemokine Transcription in
Tumorigenesis
347
Jinming Yang and Ann J. Richmond
1.
Introduction
348
2.
Development of NF-kB Reporter Model for Tumors
348
3.
Bioluminescent
Imaging of Intratumor Signaling of Anesthetized Mice
349
4.
Cell-Based Assays for Kinase and Transcriptional Activity In Vitro
352
5.
Peripheral Spying of Intratumoral Signaling of Conscious Mice
353
Acknowledgments
354
References
354
18.
Analysis of Chemokine Receptor Endocytosis
and Intracellular Trafficking
357
Tom Kershaw,
Silène T. Wavre-Shapton,
Nathalie
Signorét,
and Mark Marsh
1.
Introduction ...-o
2.
Receptor Detection
,¿n
362
4.
Monitoring Receptor Endocytosis 364
5.
Monitoring Receptor Recycling 366
6.
Monitoring Receptor Degradation 36g
7.
Electron Microscopy Analysis of Receptor Internalization 371
Contents xi
Acknowledgments
375
References
375
19.
Measuring the Proximity of
Т
-Lymphocyte CXCR4 and TCR by
Fluorescence Resonance Energy Transfer (FRET)
379
Ashok Kumar, Kimberly
N.
Kremer, Olivia L. Sims, and Karen E.
Hedin
1.
Introduction
380
2.
Assaying CXCR4-TCR Proximity via the PE/APC mAb FRET Approach
386
3.
Assaying CXCR4-TCR Proximity via the CFP/YFP Fusion
Protein Approach
392
4.
Concluding Remarks
396
References
396
20.
Expression of CXCR4, a G-Protein-Coupled Receptor for CXCL12
in Yeast: Identification of New-Generation Inverse Agonists
399
Barry J. Evans, Zixuan Wang, James R. Broach, Shinya Oishi,
Nobutaka
Fujii,
and Stephen C. Peiper
1.
Introduction
400
2.
Methods and Discussion
402
3.
Summary
408
References
410
21.
Ubiquitination of Chemokine Receptors
413
Adriano
Marchese
1.
Introduction
414
2.
Cell Culture and Transfections
416
3.
Agonist Treatment and Ubiquitination Assay
417
4.
E3 Ubiquitin
Ligase
AIP4 Mediates Ubiquitination of CXCR4
419
References
421
Author Index
423
Subject Index
451
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV035645139 |
| ctrlnum | (OCoLC)642998734 (DE-599)BVBBV035645139 |
| edition | 1. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01237nam a2200325 cc4500</leader><controlfield tag="001">BV035645139</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20110803 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">090724s2009 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123749086</subfield><subfield code="9">978-0-12-374908-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)642998734</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV035645139</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-91G</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Chemokines</subfield><subfield code="n">A</subfield><subfield code="c">ed. by Tracy M. Handel ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2009</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XLIX, 459 S., [9] Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">460</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">...</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Handel, Tracy M.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV035645025</subfield><subfield code="g">1</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in enzymology</subfield><subfield code="v">460</subfield><subfield code="w">(DE-604)BV000000938</subfield><subfield code="9">460</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017699863&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-017699863</subfield></datafield></record></collection> |
| id | DE-604.BV035645139 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:42:20Z |
| institution | BVB |
| isbn | 9780123749086 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017699863 |
| oclc_num | 642998734 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-12 |
| owner_facet | DE-355 DE-BY-UBR DE-20 DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-12 |
| physical | XLIX, 459 S., [9] Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Chemokines A ed. by Tracy M. Handel ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2009 XLIX, 459 S., [9] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 460 Methods in enzymology ... Handel, Tracy M. Sonstige oth (DE-604)BV035645025 1 Methods in enzymology 460 (DE-604)BV000000938 460 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017699863&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chemokines Methods in enzymology |
| title | Chemokines |
| title_auth | Chemokines |
| title_exact_search | Chemokines |
| title_full | Chemokines A ed. by Tracy M. Handel ... |
| title_fullStr | Chemokines A ed. by Tracy M. Handel ... |
| title_full_unstemmed | Chemokines A ed. by Tracy M. Handel ... |
| title_short | Chemokines |
| title_sort | chemokines |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017699863&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035645025 (DE-604)BV000000938 |
| work_keys_str_mv | AT handeltracym chemokinesa |