Chemical modifications of aldo-keto reductases:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 146 S. Ill., graph. Darst. 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV035547216 | ||
| 003 | DE-604 | ||
| 005 | 20090603 | ||
| 007 | t | ||
| 008 | 090603s2005 gw ad|| m||| 00||| eng d | ||
| 015 | |a 06,H02,1124 |2 dnb | ||
| 016 | 7 | |a 976728036 |2 DE-101 | |
| 035 | |a (OCoLC)181487953 | ||
| 035 | |a (DE-599)BVBBV035547216 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
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| 100 | 1 | |a Richter, Michael |d 1972- |e Verfasser |0 (DE-588)130436267 |4 aut | |
| 245 | 1 | 0 | |a Chemical modifications of aldo-keto reductases |c vorgelegt von Michael Richter |
| 264 | 1 | |c 2005 | |
| 300 | |a IV, 146 S. |b Ill., graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Leipzig, Univ., Diss., 2005 | ||
| 650 | 0 | 7 | |a Aldo-Keto Reductasen |0 (DE-588)4785721-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Modifizierung |0 (DE-588)4299539-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Aldo-Keto Reductasen |0 (DE-588)4785721-3 |D s |
| 689 | 0 | 1 | |a Modifizierung |0 (DE-588)4299539-5 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017603182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-017603182 | |
Datensatz im Suchindex
| _version_ | 1808135046559694848 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
I
TABLE
OF
CONTENTS
CHAPTER
1
1
E
XPRESSED
P
ROTEIN
L
IGATION
TO
OBTAIN
S
ELECTIVELY
M
ODIFIED
A
LDO
/K
ETO
R
EDUCTASES
1
1.1
ABSTRACT
2
1.2
INTRODUCTION
2
1.3
THE
ALDO/KETO
REDUCTASE
SUPERFAMILY
3
1.3.1
STRUCTURE
4
1.3.2
CATALYTIC
MECHANISM
5
1.3.3
COFACTOR
BINDING
5
1.3.4
SUBSTRATE
SPECIFICITY
6
1.4
EXPRESSED
PROTEIN
LIGATION
6
1.4.1
THE
IMPACT-SYSTEM
7
1.5
MODIFICATION
OF
ALDO/KETO
REDUCTASES
BY
EPL
7
1.5.1
EXAMPLES
OF
SUCCESSFULLY
MODIFIED
OXIDOREDUCTASES
8
1.6
PERSPECTIVES
10
1.7
ACKNOWLEDGEMENTS
10
CHAPTER
2
11
E
XPRESSED
PROTEIN
LIGATION
:
M
ETHOD
AND
APPLICATIONS
11
2.1
ABSTRACT
12
2.2 INTRODUCTION
12
2.3
NATIVE
CHEMICAL
LIGATION
14
2.4
THIOESTER
FORMATION
16
2.5 RECOMBINANT
GENERATION
OF
PROTEINS
WITH
C-TERMINAL
THIOESTER
OR
N
TERMINAL
CYS
18
2.5.1
INTEINS
AND
THEIR
USE
IN
PROTEIN
CHEMISTRY
18
2.5.2
SPLICING
MECHANISM
20
2.5.3 CLASSIFICATION
OF
INTEINS
23
2.5.4
THREE
DIMENSIONAL
STRUCTURES
OF
INTEINS
23
2.5.5
FORMATION
OF
C-TERMINAL
THIOESTER-ACTIVATED
PROTEINS
24
II
TABLE
OF
CONTENTS
2.5.6
IMPACT-SYSTEM
25
2.5.7
CHOICE
OF
THIOLS
27
2.5.8
GENERATION
OF
ACYS
PROTEINS
27
2.5.9
EXPRESSED
PROTEIN
LIGATION
(EPL)
28
2.6
APPLICATIONS
OF
EXPRESSED
PROTEIN
LIGATION
30
2.6.1
SITE
SPECIFIC
PROTEIN
MODIFICATIONS
30
2.6.2
INTERMOLECULAR
PROTEIN
SPLICING
IN
TRANS
TO
STUDY
PROTEIN-PROTEIN
INTERACTION
32
2.6.3
GENERATION
OF
CYCLIC
PEPTIDES
AND
PROTEINS
33
2.6.4
GENERATION
OF
CYTOTOXIC
PROTEINS
36
2.6.5
SEGMENTAL
ISOTOPIC
LABELLING
37
2.7
ALTERNATIVE
LIGATION
METHODS
38
2.7.1
NCL
WITH
CYS-MIMETICS
39
2.7.2
NCL
COMBINED
WITH
DESULFURIZATION
39
2.7.3
STAUDINGER
LIGATION
39
2.7.4
EXPRESSED
ENZYMATIC
LIGATION
40
2.8
CONCLUDING
REMARKS
42
2.9
ACKNOWLEDGEMENTS
42
CHAPTERS
43
S
ITE
SPECIFIC
BIOTINYLATION
OF
THE
HUMAN
ALDO
/
KETO
REDUCTASE
AKR1
AL
FOR
IMMOBILIZATION
43
3.1
ABSTRACT
44
3.2
INTRODUCTION
44
3.3
RESULTS
AND
DISCUSSION
47
3.4
CONCLUSIONS
52
3.5
EXPERIMENTAL
53
3.5.1
GENERAL
53
3.5.2
DNA
CONSTRUCTS
54
3.5.3
FUSION
PROTEIN
EXPRESSION
IN
E.
COLL
54
3.5.4
PURIFICATION
AND
ISOLATION
OF
THE
PROTEIN
A-THIOESTER
55
3.5.5
ENZYMATIC
ASSAY
55
TABLE
OF
CONTENTS
III
3.5.6
SYNTHESIS
AND
ANALYSIS
OF
THE
BIOTINYLATED
PEPTIDE
CYS-LYS(AHX-AHX-
BIOTIN)-NH
2
56
3.5.7
MONOLABELLING
OF
AKR1A1
BY
ATTACHMENT
OF
THE
BIOTINYLATED
PEPTIDE
57
3.5.8
SDS-PAGE
AND
WESTERN
BLOTTING
57
3.5.9
PURIFICATION
AND
IMMOBILIZATION
OF
THE
ARTIFICIAL
PROTEIN
58
3.6
ACKNOWLEDGEMENTS
58
CHAPTER
4
59
E
NGINEERING
OF
A
LDO
/K
ETO
R
EDUCTASES
FOR
S
ITE
S
PECIFIC
I
MMOBILIZATION
59
4.1
ABSTRACT
60
4.2
INTRODUCTION
60
4.3
RESULTS
64
4.3.1
MOLECULAR
MODELLING
OF
AKR
MEMBERS
TO
INVESTIGATE
THE
SITES
FOR
ENGINEERING
AND
IMMOBILIZATION
64
4.3.2
CLONING
OF
THE
RAT
3A-HYDROXYSTEROID
DEHYDROGENASE
AKR1C9
68
4.3.3
MUTAGENESIS
OF
THE
AKR1A1
C-TERMINAL
REGION
72
4.3.4
COFACTOR
ANALOGUES
75
4.4
DISCUSSION
76
4.5
MATERIALS
AND
METHODS
78
4.5.1
GENERAL
78
4.5.2
DNA
CONSTRUCTS
79
4.5.3
FUSION
PROTEIN
EXPRESSION
IN
E.
COLL
79
4.5.4
ISOLATION
AND
PURIFICATION
AND
OF
THE
PROTEIN
A-THIOESTER
80
4.5.5
MUTAGENESIS
OF
THE
C-TERMINUS
OF
THE
HUMAN
AKR1A1
80
4.5.6
ENZYMATIC
ASSAY
81
4.5.7 SDS-PAGE
81
4.5.8
SYNTHESIS
OF
3-CARBAMOYL-1-(2-CARBOXYETHYL)PYRIDINIUM
BROMIDE
81
4.5.9
NMR-SPECTROSCOPY
82
4.5.10
MASS
SPECTROMETRY
82
4.5.11
THIN
LAYER
CHROMATOGRAPHY
82
4.5.12
MOLECULAR
MODELS
83
4.6
CONCLUSIONS
83
4.7
ACKNOWLEDGEMENTS
83
IV
TABLE
OF
CONTENTS
CHAPTER
5
85
S
PECIFIC
I
MMOBILIZATION
OF
THE
HUMAN
A
LDO
/K
ETO
R
EDUCTASE
AKR1
AL
85
5.1
ABSTRACT
86
5.2
INTRODUCTION
87
5.3
RESULTS
90
5.4
DISCUSSION
98
5.5
CONCLUSIONS
101
5.6
MATERIALS
AND
METHODS
101
5.6.1
GENERAL
101
5.6.2
DNA
CONSTRUCTS
102
5.6.3
FUSION
PROTEIN
EXPRESSION
IN
E.
COLL
102
5.6.4
SDS-PAGE
AND
SILVER
STAINING
103
5.6.5
PURIFICATION
AND
ISOLATION
OF
THE
PROTEIN
A-THIOESTER
104
5.6.6
ENZYMATIC
ASSAY
104
5.6.7
SYNTHESIS
AND
CHARACTERIZATION
OF
THE
CYS
MODIFIED
TENTAGEL-BEADS
105
5.6.8
LIGATION
OF
AKR1A1
ON
THE
CYS
MODIFIED
TENTAGEL-BEADS
106
5.6.9
EDMAN
SEQUENCING
106
5.6.10
IMMOBILIZATION
OF
N-TERMINALLY
HIS
TAGGED
AKR1A1
ONTO
HIS-SELECT
HC
NICKEL
COATED
96-WELL
PLATES
106
5.7
ACKNOWLEDGEMENTS
107
REFERENCES
108
ABBREVIATIONS
125
CURRICULUM
VITAE
128
PUBLICATIONS
129
POSTERS
130
ORAL
PRESENTATIONS
131
DANKSAGUNG
132
SUMMARY
133
ZUSAMMENFASSUNG
DER
WISSENSCHAFTLICHEN
ERGEBNISSE
ZUR
DISSERTATION
139 |
| any_adam_object | 1 |
| author | Richter, Michael 1972- |
| author_GND | (DE-588)130436267 |
| author_facet | Richter, Michael 1972- |
| author_role | aut |
| author_sort | Richter, Michael 1972- |
| author_variant | m r mr |
| building | Verbundindex |
| bvnumber | BV035547216 |
| ctrlnum | (OCoLC)181487953 (DE-599)BVBBV035547216 |
| dewey-full | 572.79 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.79 |
| dewey-search | 572.79 |
| dewey-sort | 3572.79 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV035547216 |
| illustrated | Illustrated |
| indexdate | 2024-08-23T00:12:33Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017603182 |
| oclc_num | 181487953 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | IV, 146 S. Ill., graph. Darst. 30 cm |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| record_format | marc |
| spelling | Richter, Michael 1972- Verfasser (DE-588)130436267 aut Chemical modifications of aldo-keto reductases vorgelegt von Michael Richter 2005 IV, 146 S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Leipzig, Univ., Diss., 2005 Aldo-Keto Reductasen (DE-588)4785721-3 gnd rswk-swf Modifizierung (DE-588)4299539-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Aldo-Keto Reductasen (DE-588)4785721-3 s Modifizierung (DE-588)4299539-5 s b DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017603182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Richter, Michael 1972- Chemical modifications of aldo-keto reductases Aldo-Keto Reductasen (DE-588)4785721-3 gnd Modifizierung (DE-588)4299539-5 gnd |
| subject_GND | (DE-588)4785721-3 (DE-588)4299539-5 (DE-588)4113937-9 |
| title | Chemical modifications of aldo-keto reductases |
| title_auth | Chemical modifications of aldo-keto reductases |
| title_exact_search | Chemical modifications of aldo-keto reductases |
| title_full | Chemical modifications of aldo-keto reductases vorgelegt von Michael Richter |
| title_fullStr | Chemical modifications of aldo-keto reductases vorgelegt von Michael Richter |
| title_full_unstemmed | Chemical modifications of aldo-keto reductases vorgelegt von Michael Richter |
| title_short | Chemical modifications of aldo-keto reductases |
| title_sort | chemical modifications of aldo keto reductases |
| topic | Aldo-Keto Reductasen (DE-588)4785721-3 gnd Modifizierung (DE-588)4299539-5 gnd |
| topic_facet | Aldo-Keto Reductasen Modifizierung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017603182&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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