Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Leipzig
Helmholtz Centre for Environmental Research, UFZ
2009
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| Schriftenreihe: | PhD dissertation
2009,3 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 138, XXVIII S. Ill., graph. Darst. 25 cm |
Internformat
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| 245 | 1 | 0 | |a Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos |c Ulrike Gündel |
| 264 | 1 | |a Leipzig |b Helmholtz Centre for Environmental Research, UFZ |c 2009 | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: Proteomics approach for toxicity assessment in zebrafish (Danio rerio) embryos
Autor: Gündel, Ulrike
Jahr: 2009
General Structure
Contents
List of Abbreviations i
Chapter 1 General Introduction 1
Chapter 2 Methodology 15
Chapter 3 Proteome Analysis During the Development of Zebrafish 29
Chapter 4 Proteomics Study of Toxic Stress in Early Embryonal
Stages of the Zebrafish 51
Chapter 5 Proteomics Study of Toxic Stress in Zebrafish Larvae 67
Chapter 6 Cathepsins as a New Family of Biomarkers in Zebrafish
Embryos for Normal Embryonal Development and Toxic
Exposure 101
Chapter 7 Summary - Conclusion 119
Zusammenfassung 125
References 131
Appendix I
Contents
List of Abbreviations i
1 General Introduction 1
2 Methodology 15
2.1 Physico-Chemical Properties of Characterised Toxicants 16
2.2 Fish Culture, Embryo Collection and Exposure Conditions 17
2.2.1 Fish Embryo Test (DarT) 17
2.2.2 Effect Determination in Larvae 19
2.2.3 Exposure Conditions for the Analysis of Toxic Responses
at the Proteome Level 20
2.3 Protein Analysis 21
2.3.1 Protein Sample Preparation 21
2.3.2 One Dimensional SDS-PAGE 21
2.3.3 Two-Dimensional Gelelectrophoresis (2-DE) 22
2.3.4 Image Analysis and Statistics 23
2.3.5 Identification of Proteins 24
2.3.6 Immunoblot Analysis 24
2.4 Cathepsin Enzymatic Activity Analysis (Chapter 6) 25
2.4.1 Danio rerio Embryo Collection for Enzymatic Measurements
at Different Developmental Stages 25
2.4.2 Exposure Conditions to Study in vivo Effects of Leupeptin,
DNOC, Ethanol and Rotenone on Cathepsin Activities 25
2.4.3 Protein Isolation from Danio rerio Embryos 26
2.4.4 Cathepsins Assays (CatC, CatH, CatL) 26
3 Proteome Analysis During the Development of Zebrafish 29
3.1 Introduction 30
3.2 Results 32
3.2.1 Characterisation of the Protein Composition of Zebrafish
Embryos at Different Developmental Stages 32
3.2.2 Identification of Proteins in 2 dpf old Embryos 35
3.2.3 Immunoblot Analysis with Anti-Vtg Antibody 40
3.2.4 Identification of Proteins in Five Day old Larvae 42
3.3 Discussion 44
3.3.1 Protein Pattern Changes During Embryonal Development 44
3.3.2 Ratio of Yolk-to Cellular Proteins Decreases During
Embryonal Development 47
4 Proteomics Study of Toxic Stress in Early Embryonal Stages of
the Zebrafish 51
4.1 Introduction 52
4.2 Results 54
4.2.1 Danio rerio Fish Embryo Test with Ethanol 54
4.2.2 2-DE Analysis of Zebrafish Embryos Exposed to two
Different Ethanol Concentrations 56
4.3 Discussion 62
5 Proteomics Study of Toxic Stress Zebrafish Lavae 67
5.1 Introduction 68
5.2 Results 69
5.2.1 Optimisation of 2-DE Method Concerning Age of Larvae 69
5.2.2 Analysis of Morphological and Physiological Effects
in Zebrafish Larvae Treated with DNOC, Rotenone and
Diclofenac 72
5.2.3 Toxicoproteomics Approaches with Zebrafish Larvae
Using three Toxicants: Rotenone, DNOC, Diclofenac 75
5.3 Discussion 87
6 Cathepsins as a new Family of Biomarkers in Zebrafish
Embryos for Normal Embryonal Development and Toxic Exposure 101
6.1 Introduction 102
6.2 Results 103
6.2.1 Cathepsin Activities in Embryonal and Larval Stages
of the Zebrafish 103
6.2.2 Leupeptin as Positive Control for direct in vivo Cathepsin
Inhibition 106
6.2.3 Effect of three Chemicals on the in vivo Activity of the
Cathepsins L, C and H 108
6.2.4 Concentration Dependence of Effects on Cathepsin
Activity 110
6.3 Discussion 112
6.3.1 Cathepsin L, C and H Activity During Embryogenesis 113
6.3.2 Toxicant Related Changes in Cathepsins Activities 115
7 Summary and Conclusions 119
Zusammenfassung 125
References 131
Appendix I
I Results of Toxicoproteomics Studies II
II Results of Effect Screening Assays with Embryonal and Larval
Stages of the Zebrafish XVI11
Publications/Conference Contributions
Erklarungen
Danksagung
Lebenslauf
|
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| spelling | Gündel, Ulrike 1980- Verfasser (DE-588)137854048 aut Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos Ulrike Gündel Leipzig Helmholtz Centre for Environmental Research, UFZ 2009 138, XXVIII S. Ill., graph. Darst. 25 cm txt rdacontent n rdamedia nc rdacarrier PhD dissertation 2009,3 Zugl.: Berlin, Humboldt-Univ., Diss., 2008 Zebrabärbling (DE-588)4079468-4 gnd rswk-swf Toxizitätstest (DE-588)4060540-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zebrabärbling (DE-588)4079468-4 s Toxizitätstest (DE-588)4060540-1 s DE-604 PhD dissertation 2009,3 (DE-604)BV035421074 2009,3 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017371735&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gündel, Ulrike 1980- Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos PhD dissertation Zebrabärbling (DE-588)4079468-4 gnd Toxizitätstest (DE-588)4060540-1 gnd |
| subject_GND | (DE-588)4079468-4 (DE-588)4060540-1 (DE-588)4113937-9 |
| title | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos |
| title_auth | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos |
| title_exact_search | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos |
| title_full | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos Ulrike Gündel |
| title_fullStr | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos Ulrike Gündel |
| title_full_unstemmed | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos Ulrike Gündel |
| title_short | Proteomics approach for toxicity assessment in Zebrafish (Danio rerio) embryos |
| title_sort | proteomics approach for toxicity assessment in zebrafish danio rerio embryos |
| topic | Zebrabärbling (DE-588)4079468-4 gnd Toxizitätstest (DE-588)4060540-1 gnd |
| topic_facet | Zebrabärbling Toxizitätstest Hochschulschrift |
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