Molecular biology and biotechnology:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Cambridge
RSC Publ.
2009
|
| Ausgabe: | 5. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIX, 604 S. Ill., graph. Darst. |
| ISBN: | 9780854041251 0854041257 |
Internformat
MARC
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| 245 | 1 | 0 | |a Molecular biology and biotechnology |c ed. by John M. Walker ... |
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| 300 | |a XIX, 604 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804138899515113472 |
|---|---|
| adam_text | Titel: Molecular biology and biotechnology
Autor: Walker, John M
Jahr: 2009
Contents
Chapter 1 Basic Molecular Biology Techniques
Ralph Rapley
1.1 Enzymes Used in Molecular Biology 1
1.2 Isolation and Separation of Nucleic Acids 3
1.2.1 Isolation of DNA 3
1.2.2 Isolation of RNA 5
1.3 Electrophoresis of Nucleic Acids 6
1.4 Restriction Mapping of DNA Fragments 8
1.5 Nucleic Acid Analysis Methods 8
1.5.1 DNA Blotting 9
1.5.2 RNA Blotting 10
1.6 Gene Probe Derivation 11
1.7 Labelling DNA Gene Probe Molecules 12
1.7.1 End Labelling of DNA Molecules 13
1.7.2 Random Primer Labelling 14
1.7.3 Nick Translation 15
1.8 The Polymerase Chain Reaction 15
References 18
Chapter 2 Molecular Cloning and Protein Expression
Stuart Harbron
2.1 Introduction 20
2.2 Host-related Issues 21
2.3 Vectors 24
2.4 Expression Systems 30
2.4.1 The pET Expression System 30
2.4.2 The pBAD Expression System 33
Molecular Biology and Biotechnology, 5th Edition
Edited by John M Walker and Ralph Rapley
£ Royal Society of Chemistry 2009
Published by the Royal Society of Chemistry, www.rsc.org
vti
viii Contents
2.5 Problems 34
2.6 Fusion Proteins 37
2.6.1 Solubility-enhancing Tags 37
2.6.2 Purification-facilitating Tags 40
2.6.3 HT Approaches 43
2.7 Other Hosts 44
2.8 Cell-free Systems 44
2.9 Conclusion 45
References 45
Chapter 3 Molecular Diagnostics
Laura J. Tafe, Claudine L. Bartels, Joel A. Lefferts and
Gregory J. Tsongalis
51
52
53
55
59
62
65
71
References 71
Chapter 4 Molecular Microbial Diagnostics
Karl-Henning Kattand
4.1 Introduction 76
4.2 Classical Microbiological Diagnosis 78
4.3 Sample Collection and Nucleic Acid Purification 79
4.3.1 Sample Collection and Transport 79
4.3.2 Extraction of Nucleic Acids 80
4.3.3 Manual Extraction of Nucleic Acids 81
4.3.4 Automated Extraction of Nucleic Acids 81
4.4 Nucleic Acid Amplification Techniques 81
4.4.1 Polymerase Chain Reaction (PCR) 81
4.4.2 The Contamination Problem 82
4.4.3 Reverse PCR - cDNA Synthesis 82
4.4.4 Nested PCR 83
4.4.5 Real-time PCR 83
4.4.6 Visualisation of Real-time PCR Amplification 84
4.4.7 Real-time PCR Equipment 86
4.4.8 Real-time Quantitative PCR 86
4.4.9 Determination of Viral Load in Clinical
Microbiology 87
3.1 Introduction
3.2 Technologies
3.3 The Infectious Disease Paradigm
3.4 Genetics
3.5 Hematology
3.6 Oncology
3.7 Pharmacogenomics
3.8 Conclusion
Contents ix
4.4.10 Internal Controls in Microbiological
Real-time qPCR 87
4.4.11 Multiplex Real-time PCR 88
4.4.12 Melting Curve Analysis 88
4.4.13 Genotyping 89
4.5 Other Techniques Used in Clinical Microbiology 90
4.5.1 Hybridisation Techniques 90
4.5.2 Nucleic Acid-based Typing of Bacteria 93
4.5.3 Pyrosequencing 97
4.5.4 TaqMan Low-density Arrays (TLDAs) 98
4.6 Selected Examples of Clinical Nucleic
Acid-based Diagnosis 99
4.6.1 Central Nervous System (CNS) Disease 99
4.6.2 Respiratory Infections 100
4.6.3 Hepatitis 100
4.6.4 Gastroenteritis 101
4.6.5 Sexually Transmitted Diseases 102
4.6.6 HIV Infection and AIDS 103
4.6.7 Bacterial Antibiotic Resistance and Virulence
Factor Genes 104
4.7 Conclusion and Future Challenges 106
References 107
Chapter 5 Genes and Genomes
David B. Whitehouse
5.1 Introduction 112
5.1.1 Background 112
5.2 Key DNA Technologies 115
5.2.1 Molecular Cloning Outline 115
5.2.2 Cloning Vectors 115
5.2.3 The Cloning Process 118
5.2.4 DNA Libraries 120
5.3 The Polymerase Chain Reaction (PCR) 122
5.3.1 Steps in the PCR 123
5.3.2 PCR Primer Design and Bioinformatics 125
5.3.3 Reverse Transcriptase PCR (RT-PCR) 126
5.3.4 Quantitative or Real-time PCR 126
5.4 DNA Sequencing 128
5.4.1 Dideoxynucleotide Chain
Terminators 129
5.4.2 Sequencing Double-stranded DNA 129
5.4.3 PCR Cycle Sequencing 130
5.4.4 Automated DNA Sequencing 130
5.4.5 Pyrosequencing 131
Contents
5.5 Genome Analysis 131
5.5.1 Mapping and Identifying Genes 131
5.5.2 Tools for Genetic Mapping 132
5.5.3 Mutation Detection 139
5.6 Genome Projects Background 144
5.6.1 Mapping and Sequencing Strategies 144
5.7 Gene Discovery and Localisation 149
5.7.1 Laboratory Approaches 149
5.7.2 Bioinformatics Approaches 150
5.8 Future Directions 152
References 153
Chapter 6 The Biotechnology and Molecular Biology of Yeast
Brendan P. G. Curran and Virginia C. Bugeja
6.1 Introduction 159
6.2 The Production of Heterologous Proteins by Yeast 161
6.2.1 The Yeast Hosts 161
6.2.2 Assembling and Transforming Appropriate
DNA Constructs into the Hosts 162
6.2.3 Ensuring Optimal Expression of the Desired
Protein 165
6.3 From Re-engineering Genomes to Constructing Novel
Signal and Biochemical Pathways 170
6.3.1 Large-scale Manipulation of Mammalian and
Bacterial DNA 170
6.3.2 Novel Biological Reporter Systems 176
6.3.3 Novel Biochemical Products Include
Humanised EPO 179
6.4 Yeast as a Paradigm of Eukaryotic Cellular Biology 187
6.4.1 Genomic Insights 187
6.4.2 Transeriptomes, Profeomes and Metabolomes
and Drug Development 188
6.4.3 Systems Biology 190
6.5 Future Prospects 191
References 191
Chapter 7 Metabolic Engineering
Stefan Kempa, Dirk Walther, Oliver Ebenhoeh and Wolfram
Weckwerth
7.1 Introduction 196
7.2 Theoretical Approaches for Metabolic Networks 197
7.2.1 Kinetic Modelling 198
Contents
7.2.2 Metabolic Control Analysis (MCA),
Elementary Flux Modes (EFM) and
Flux-balance Analysis (FBA) 202
7.3 Experimental Approaches for Metabolic Engineering 208
7.3.1 Tools for Metabolic Engineering 208
7.3.2 Metabolomics 209
7.3.3 Metabolomics in the Context of Metabolic
Engineering 210
7.4 Examples in Metabolic Engineering 211
7.4.1 Metabolic Engineering of Plants 211
7.4.2 Acetate Metabolism and Recombinant Protein
Synthesis in E. coli - a Test Case for Metabolic
Engineering 213
7.4.3 Metabolic Flux Analysis and a Bioartificial Liver 213
7.5 Omics Technologies Open New Perspectives for
Metabolic Engineering 214
7.6 Acknowledgement 215
References 215
Chapter 8 Bionanotechnology
David W. Wright
8.1 Introduction 220
8.2 Semiconductor Quantum Dots 222
8.2.1 Quantum Confinement Effects 222
8.2.2 Biotechnological Applications of Fluorescent
Semiconductor Quantum Dots 224
8.3 Magnetic Nanoparticles 226
8.3.1 Nanoscaling Laws and Magnetism 226
8.3.2 Biotechnological Applications of Magnetic
Nanoparticles 228
8.4 Zerovalent Noble Metal Nanoparticles 232
8.4.1 Nanoscale Properties of Zerovalent Nobel
Metal Nanoparticles 232
8.4.2 Bionanotechnology Application of Zerovalent
Noble Metal Nanoparticles 234
8.5 Making Nanoscale Structures Using Biotechnology 236
8.6 Conclusions 241
References 241
Chapter 9 Molecular Engineering of Antibodies
James D. Marks
9.1 Introduction 245
9.2 Antibodies as Therapeutics 246
Contents
9.3 Antibody Structure and Function 250
9.4 Chimeric Antibodies 251
9.5 Antibody Humanization 255
9.6 Antibodies from Diversity Libraries and Display
Technologies 256
9.6.1 Antibody Phage Display 257
9.6.2 Alternative Display Technologies 262
9.7 Engineering Antibody Affinity 263
9.8 Enhancing Antibody Potency 264
9.9 Conclusion 265
References 265
Chapter 10 Plant Biotechnology
Michael G. K. Jones
10.1 Introduction 272
10.2 Applications of Molecular Biology to Speed Up the
Processes of Crop Improvement 273
10.2.1 Molecular Maps of Crop Plants 273
10.2.2 Molecular Markers 274
10.2.3 Types of Molecular Markers 274
10.2.4 Marker-assisted Selection 275
10.2.5 Examples of Marker-assisted Selection 276
10.2.6 Molecular Diagnostics 277
10.2.7 DNA Fingerprinting, Variety Identification 278
10.2.8 DNA Microarrays 279
10.2.9 Bioinformatics 279
10.3 Transgenic Technologies 279
10.3.1 Agrobacterium-medmted Transformation 280
10.3.2 Selectable Marker and Reporter Genes 280
10.3.3 Particle Bombardment 281
10.4 Applications of Transgenic Technologies 281
10.5 Engineering Crop Resistance to Herbicides 283
10.6 Engineering Resistance to Pests And Diseases 284
10.6.1 Insect Resistance 284
10.6.2 Engineered Resistance to Plant Viruses 285
10.6.3 Resistance to Fungal Pathogens 287
10.6.4 Natural Resistance Genes 288
10.6.5 Engineering Resistance to Fungal Pathogens 290
10.6.6 Resistance to Bacterial Pathogens 291
10.6.7 Resistance to Nematode Pathogens 292
10.7 Manipulating Male Sterility 292
10.8 Tolerance to Abiotic Stresses 293
10.9 Manipulating Quality 294
10.9.1 Prolonging Shelf Life 294
Contents xiti
10.9.2 Nutritional and Technological Properties 294
10.9.3 Manipulation of Metabolic Partitioning 297
10.10 Production of Plant Polymers and Biodegradable
Plastics 298
10.11 Plants as Bioreactors: Biopharming and
Neutraceuticals 298
10.11.1 Edible Vaccines 298
10.11.2 Production of Antibodies in Plants 299
10.11.3 Plant Neutraceuticals 299
10.12 Plant Biotechnology in Forestry 300
10.13 Intellectual Property 300
10.14 Public Acceptance 301
10.15 Future Prospects 302
References 303
Chapter 11 Biotechnology-based Drug Discovery
K. K. Jain
11.1 Introduction to Drug Discovery 307
11.1.1 Basics of Drug Discovery in the Biopharma-
ceutical Industry 307
11.1.2 Historical Landmarks in Drug Discovery and
Development 308
11.1.3 Current Status of Drug Discovery 309
11.2 New Biotechnologies for Drug Discovery 310
11.3 Genomic Technologies for Drug Discovery 310
11.3.1 SNPs in Drug Discovery 311
11.3.2 Gene Expression Profiling 312
11.3.3 Limitations of Genomics for Drug Discovery
and Need for Other Omics 312
11.4 Role of Proteomics in Drug Discovery 313
11.4 J Proteins as Drug Targets 313
11.4.2 Protein Expression Mapping by 2D Gel
Electrophoresis 314
11.4.3 Liquid Chromatography-based Drug
Discovery 314
11.4.4 Matrix-assisted Laser Desorption, lonisation
Mass Spectrometry 314
11.4.5 Protein-Protein Interactions 315
11.4.6 Use of Proteomic Technologies for
Important Drug Targets 316
11.5 Metabolomic and Metabonomic Technologies for
Drug Discovery 317
11.6 Role of Nanobiotechnology
in Drug Discovery 318
xiv Contents
11.6.1 Nanobiotechnology for Target Validation 318
11.6.2 Nanotechnology-based Drug Design at
Cell Level 318
11.6.3 Nanomaterials as Drug Candidates 319
11.7 Role of Biomarkers in Drug Discovery 320
11.8 Screening in Drug Discovery 320
11.8.1 Cell-based Screening System 321
11.8.2 Receptor Targets: Human versus Animal
Tissues 321
11.8.3 Tissue Screening 322
11.9 Target Validation Technologies 322
11.9.1 Animal Models for Genomics-based
Target Validation Methods 322
11.9.2 Role of Knockout Mice in Drug Discovery 323
11.10 Antisense for Drug Discovery 323
11.10.1 Antisense Oligonucleotides for Drug
Target Validation 324
11.10.2 Aptamers 324
11.10.3 RNA as a Drug Target 325
11.10.4 Ribozymes 325
11.11 RNAi for Drug Discovery 326
11.11.1 Use of siRNA Libraries to Identify Genes
as Therapeutic Targets 327
11.11.2 RNAi as a Tool for Assay Development 328
11.11.3 Challenges of Drug Discovery with
RNAi 328
11.11.4 Role of MicroRNA in Drug Discovery 329
11.12 Biochips and Microarrays
for Drug Discovery 329
11.12.1 Finding Lead Compounds 330
11.12.2 High-throughput cDNA Microarrays 330
11.12.3 Use of Gene Expression Data to Find New
Drug Targets 330
11.12.4 Investigation of the Mechanism of Drug
Action 331
11.13 Applications of Bioinformatics
in Drug Discovery 331
II. 13.1 Combination oflnSilico and In vitro Studies 332
11.14 Role of Model Organisms in Drug Discovery 333
11.15 Chemogenomic Approach to Drug Discovery 334
11.16 Virtual Drug Development 334
11.17 Role of Biotechnology in Lead Generation and
Validation 335
11.18 Conclusion 335
References 336
Contents
Chapter 12 Vaccines
Niall McMullan
12.1 An Overview of Vaccines and
Vaccination 337
12.2 Types of Vaccines in Current Use 338
12.2.1 Live, Attenuated Vaccines 338
12.2.2 Inactivated Vaccines 339
12.2.3 Subunit Vaccines 340
12.3 The Need for New Vaccines 342
12.4 New Approaches to Vaccine Development 343
12.4.1 Recombinant Live Vectors 343
12.4.2 Recombinant BCG Vectors 343
12.4.3 Recombinant Salmonella Vectors 344
12.4.4 Recombinant Adenovirus Vectors 345
12.4.5 Recombinant Vaccinia Vectors 346
12.4.6 DNA Vaccines 346
12.5 Adjuvants 347
12.5.1 Immune-stimulating Complexes (ISCOMs)
and Liposomes 347
12.5.2 Freund-type Adjuvants 347
12.5.3 CpG Oligonucleotides (CpG ODNs) 348
References 349
Chapter 13 Tissue Engineering
Nils Link and Martin Fussenegger
13.1 Introduction 351
13.1.1 Economic Impact of Healthcare 351
13.1.2 Tissue Engineering 352
13.1.3 Treating Disease Through Tissue
Engineering 353
13.2 Cell Types 356
13.2.1 Embryonic Stem Cells 356
13.2.2 Adult Stem Cells 360
13.2.3 Mature Cells 361
13.3 Extracellular Matrix 362
13.3.1 Biological Extracellular Matrices 362
13.3.2 Artificial Extracellular Matrices 364
13.4 Tissue Engineering Concepts 369
13.4.1 Cultivation of Artificial Tissues 369
13.4.2 Design of Scaffold-free Tissues 372
13.5 Conclusions 373
References 373
xvj Contents
Chapter 14 Transgenesis
Elizabeth J. Cartwright and Xin Wang
14.1 Introduction 390
14.1.1 From Gene to Function 390
14.2 Transgenesis by DNA Pronuclear Injection 391
14.2.1 Generation of a Transgenic Mouse 391
14.2.2 Summary of Advantages and Disadvantages
of Generating Transgenic Mice by
Pronuclear Injection of DNA 397
14.3 Gene Targeting by Homologous Recombination
in Embryonic Stem Cells 397
14.3.1 Basic Principles 398
14.3.2 Generation of a Knockout Mouse 400
14.3.3 Summary of Advantages and Disadvantages
of Generating Gene Knockout Mice 404
14.4 Conditional Gene Targeting 404
14.4.1 Generation of a Conditional Knockout
Mouse Using the Cre-loxP System 406
14.4.2 Chromosomal Engineering Using the
Cre-loxP System 410
14.4.3 Summary of Advantages and
Disadvantages of Conditional Gene
Targeting 410
14.5 Phenotypic Analysis of Genetically
Modified Mice 411
14.6 Ethical and Animal Welfare Considerations 412
14.7 Conclusions 414
14.8 Acknowledgements 414
References 415
Chapter 15 Protein Engineering
John Adair and Duncan McGregor
15.1 Introduction 418
15.1.1 Protein Structures 419
15.2 Tools of the Trade 420
15.2.1 Sequence Identification 420
15.2.2 Structure Determination and Modelling 420
15.2.3 Sequence Modification 421
15.2.4 Production 432
15.2.5 Analysis 433
15.3 Applications 434
15.3.1 Point Mutations 434
15.3.2 Domain Shuffling (Linking, Swapping
and Deleting) 435
Contents xvii
15.3.3 Whole Protein Shuffling 441
15.3.4 Protein-Ligand Interactions 441
15.3.5 Towards De Novo Design 442
15.4 Conclusions and Future Directions 443
References 447
Chapter 16 Immobilisation of Enzymes and Cells
Gordon F. Bicker staff
16.1 Introduction 454
16.2 Biocatalysts 455
16.2.1 Enzymes 455
16.2.2 Ribozymes, Deoxyribozymes and Ribosomes 459
16.2.3 Splicesomes 460
16.2.4 Abzymes 461
16.2.5 Multienzyme Complexes 462
16.2.6 Cells 466
16.2.7 Biocatalyst Selection 468
16.3 Immobilisation 469
16.3.1 Choice of Support Material 470
16.3.2 Choice of Immobilisation Procedures 474
16.4 Properties of Immobilised Biocatalysts 483
16.4.1 Stability 483
16.4.2 Catalytic Activity 484
16.4.3 Coenzyme Regeneration 485
16.5 Applications 487
References 489
Chapter 17 Downstream Processing
Daniel G. BraceweU, Mohammad AU S. Mumtaz and
C. Mark Smales
17.1 Introduction 492
17.2 Initial Considerations and Primary Recovery 493
17.2.1 Centrifugation and Filtration 494
17.2.2 Ceil Lysis 494
17.2.3 Recovery of Material from Inclusion Bodies 495
17.3 Protein Precipitation 496
17.4 Chromatography 497
17.4.1 Ion-exchange Chromatography (IEX) 499
17.4.2 Affinity Chromatography 500
17.4.3 Hydrophobic Interaction Chromatography
(HIC) 501
17.4.4 Gel Filtration Chromatography 501
17.5 Alternatives to Packed Bed Chromatography 502
xvjii Contents
17.5.1 Expanded Bed Adsorption 502
17.5.2 Aqueous Two-phase Extraction 503
17.5.3 Membrane Chromatography and Filtration 503
17.5.4 Crystallisation 504
17.5.5 Monolith Columns 505
17.6 Design of Biomolecules for Downstream Processing 505
17.7 Scaledown Methods 506
17.8 Validation and Robustness 506
17.9 Formulation and Antiviral Treatments 507
17.9.1 Formulation 507
17.9.2 Antiviral Treatments 508
17.10 Current Developments and Future Directions 509
References 510
Chapter 18 Biosensors
Martin F. Chaplin
18.1 Introduction 513
18.2 The Biological Reaction 518
18.3 Theory 519
18.4 Electrochemical Methods 522
18.4.1 Amperometric Biosensors 522
18.4.2 Potentiometric Biosensors 531
18.4.3 Conductimetric Biosensors 533
18.5 Piezoelectric Biosensors 534
18.6 Optical Biosensors 536
18.6.1 Evanescent Wave Biosensors 538
18.6.2 Surface Plasmon Resonance 540
18.7 Whole Cell Biosensors 542
18.8 Receptor-based Sensors 543
18.9 Conclusion 545
References 546
Chapter 19 Biofuels and Biotechnology
Jonathan R. Mielenz
19.1 Introduction 548
19.2 Production of the Major Biofuels 549
19.2.1 Corn Processing and Ethanol 550
19.2.2 Biomass Conversion for Ethanol 552
19.2.3 Biodiese! 556
19.3 Application of Biotechnology Tools to Biofuels
Processes 557
19.3.1 Improved Production of Corn Ethanol 559
19.3.2 Ethanol Production from Biomass 561
Contents xix
19.3.3 Biobutanol 572
19.3.4 Biodiesel 573
19.3.5 New Concepts 573
19.4 Future Perspectives 574
References 576
Subject Index 585
|
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| building | Verbundindex |
| bvnumber | BV035449381 |
| classification_rvk | WF 9700 |
| classification_tum | BIO 220f BIO 180f CIT 972f |
| ctrlnum | (OCoLC)441888699 (DE-599)BSZ304799718 |
| discipline | Biologie Chemie-Ingenieurwesen Biotechnologie |
| edition | 5. ed. |
| format | Book |
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| genre | 1\p (DE-588)1071861417 Konferenzschrift 1985 Hatfield gnd-content |
| genre_facet | Konferenzschrift 1985 Hatfield |
| id | DE-604.BV035449381 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:35:31Z |
| institution | BVB |
| isbn | 9780854041251 0854041257 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017369464 |
| oclc_num | 441888699 |
| open_access_boolean | |
| owner | DE-20 DE-91G DE-BY-TUM |
| owner_facet | DE-20 DE-91G DE-BY-TUM |
| physical | XIX, 604 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | RSC Publ. |
| record_format | marc |
| spelling | Molecular biology and biotechnology ed. by John M. Walker ... 5. ed. Cambridge RSC Publ. 2009 XIX, 604 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biotechnologie (DE-588)4069491-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf 1\p (DE-588)1071861417 Konferenzschrift 1985 Hatfield gnd-content Molekularbiologie (DE-588)4039983-7 s Biotechnologie (DE-588)4069491-4 s 2\p DE-604 Gentechnologie (DE-588)4071722-7 s 3\p DE-604 Walker, John M. Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017369464&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Molecular biology and biotechnology Biotechnologie (DE-588)4069491-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Gentechnologie (DE-588)4071722-7 gnd |
| subject_GND | (DE-588)4069491-4 (DE-588)4039983-7 (DE-588)4071722-7 (DE-588)1071861417 |
| title | Molecular biology and biotechnology |
| title_auth | Molecular biology and biotechnology |
| title_exact_search | Molecular biology and biotechnology |
| title_full | Molecular biology and biotechnology ed. by John M. Walker ... |
| title_fullStr | Molecular biology and biotechnology ed. by John M. Walker ... |
| title_full_unstemmed | Molecular biology and biotechnology ed. by John M. Walker ... |
| title_short | Molecular biology and biotechnology |
| title_sort | molecular biology and biotechnology |
| topic | Biotechnologie (DE-588)4069491-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Gentechnologie (DE-588)4071722-7 gnd |
| topic_facet | Biotechnologie Molekularbiologie Gentechnologie Konferenzschrift 1985 Hatfield |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017369464&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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