Forensic DNA analysis: a laboratory manual
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boca Raton [u.a.]
CRC Press
c2008
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references (p. 157-158) and index |
| Beschreibung: | xiii, 161 p. ill. 28 cm |
| ISBN: | 9781420063295 1420063294 |
Internformat
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| 650 | 4 | |a DNA fingerprinting |v Laboratory manuals | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
List of Figures vii
List of Tables ix
Welcome to the Forensic DNA Analysis Laboratory xi
Laboratory Rules xiii
Chapter 1
An Overview of Forensic DNA Analysis 1
Chapter 2
Types of Biological Samples 3
Chapter 3
Exercise 1 - DNA Extraction 7
Chapter 4
Exercise 2 - Concentration of Extracted DNA 17
Chapter 5
Exercise 3 - Microcon Concentration and Purification of Extracted DNA 23
Chapter 6
Exercise 4 - Assessing the Quality and Quantity of Isolated DNA 29
Chapter 7
Exercise 5 - DNA Analysis Using Restriction Fragment Length Polymorphisms (RFLP) 39
Chapter 8
Polymerase Chain Reaction (PCR) 39
Chapter 9
Exercise 6 - Polymerase Chain Reaction (PCR)-Based Tests: The AmpliType PM/DQA1 System 61
Chapter 10
Exercise 7 - Polymerase Chain Reaction (PCR)-Based Tests: The D1S80 System 71
v
vi Forensic DNA Analysis: A Laboratory Manual
Chapter 11
Experiment 8 - Polymerase Chain Reaction (PCR)-Based Tests: Short Tandem
Repeat (STR) Analysis 83
Chapter 12
Experiment 9 - Polymerase Chain Reaction (PCR)-Based Tests: Short Tandem Repeat (STR)
Analysis to Determine Paternity (a Case Study) 97
Chapter 13
Experiment 10 - Polymerase Chain Reaction (PCR)-Based Tests: Y-Chromosome Short Tandem
Repeat (Y-STR) Analysis (a Case Study) 105
Chapter 14
Exercise 11 - Mitochondrial DNA (mtDNA) Analysis 119
Chapter 15
Exercise 12 - Assessment of Lumigraph or Autoradiograph Data 133
Appendix A
Composition of Buffers and Solutions 139
Appendix B
Using a Micropipet 143
Appendix C
Glossary 147
References and Suggested Reading 157
Index 159
List of Figures HI
Figure 1 DNeasy Blood and Tissue Procedure for the Isolation, Purification, and Concentration of
DNA
Figure 2 Restriction Fragment Length Polymorphism (RFLP) Lumigraph (see Exercise 9) Demon¬
strating DNA Degradation
Figure 3 Restriction Fragment Length Polymorphism (RFLP) Lumigraph (see Exercise 9) Demon¬
strating DNA Degradation
Figure 4 Slot Blot Used to Determine the Quantity of Human DNA Recovered from a Sample
Figure 5 Digest Gel Demonstrating Completely and Incompletely Digested DNA Samples
Figure 6 Overview of RFLP Procedure
Figure 7 Overview of Polymerase Chain Reaction (PCR)
Figure 8 Detection of PCR Products Using the AmpliType PM/DQA1 Test and Reverse Dot Blot
Technology
Figure 9 DQA1 Typing Strip
Figure 10 HLA DQA1 Typing Results
Figure 11 PM Typing Strip
Figure 12 Profiles from GenePrint STR Systems (CSF1PO, TPOX, and TH01)
Figure 13 Capillary Electrophoresis System
Figure 14 PowerPlex Y System
Figure 15 PowerPlex Y-STR Typing Results
Figure 16 PowerPlex Y Allelic Ladder Mix
Figure 17 PowerPlex Y-STR Typing Results: Alleles Identified
Figure 18 Mitochondrial Genome in Humans
Figure 19 LI-COR Infrared DNA Analyzer (Model 4300)
Figure 20 Expanded View and List of Parts for the Gel Apparatus
Figure 21 Sharkstooth Comb
Figure 22 Rainin Classic Pipetman
vii
List of Tables HI
Table 1 DNA Contents of Biological Samples
Table 2 Physical Evidence Collected at Crime Scenes
Table 3 PCR Master Mix for D1S80 Locus
Table 4 Information on the 13 Core Short Tandem Repeat Loci Listed in CODIS
Table 5 Multiplex Reactions Containing Three Loci
Table 6 Locus Specific Information for the Geneprint STR Systems (CSF1PO, TPOX, and TH01)
Table 7 PowerPlex 16 BIO Typing Results
Table 8 Y-STR Loci of PowerPlex Y System
Table 9 Master Mix for PowerPlex Y System
Table 10 Y-STR Typing Results
Table 11 Template Amount Used in Simultaneous Bidirectional Sequencing (SBS)
Table 12 Concentration of Template DNA Recommended for Labeled Primer Cycle Sequencing
Table 13 Template-Primer Master Mix
Table 14 Mitochondrial DNA Typing Results
ix
|
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| indexdate | 2024-07-09T21:34:13Z |
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| isbn | 9781420063295 1420063294 |
| language | English |
| lccn | 2007045327 |
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| physical | xiii, 161 p. ill. 28 cm |
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| spelling | McClintock, J. Thomas Verfasser aut Forensic DNA analysis a laboratory manual J. Thomas McClintock Boca Raton [u.a.] CRC Press c2008 xiii, 161 p. ill. 28 cm txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references (p. 157-158) and index DNA Analysis Laboratory manuals DNA fingerprinting Laboratory manuals Forensic genetics Laboratory manuals DNA Fingerprinting Laboratory Manuals Forensic Medicine methods Laboratory Manuals Gerichtliche Chemie (DE-588)4202330-0 gnd rswk-swf Fingerprint-Verfahren (DE-588)4231579-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf DNS (DE-588)4070512-2 s Fingerprint-Verfahren (DE-588)4231579-7 s Gerichtliche Chemie (DE-588)4202330-0 s Methode (DE-588)4038971-6 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017314457&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | McClintock, J. Thomas Forensic DNA analysis a laboratory manual DNA Analysis Laboratory manuals DNA fingerprinting Laboratory manuals Forensic genetics Laboratory manuals DNA Fingerprinting Laboratory Manuals Forensic Medicine methods Laboratory Manuals Gerichtliche Chemie (DE-588)4202330-0 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd Methode (DE-588)4038971-6 gnd DNS (DE-588)4070512-2 gnd |
| subject_GND | (DE-588)4202330-0 (DE-588)4231579-7 (DE-588)4038971-6 (DE-588)4070512-2 |
| title | Forensic DNA analysis a laboratory manual |
| title_auth | Forensic DNA analysis a laboratory manual |
| title_exact_search | Forensic DNA analysis a laboratory manual |
| title_full | Forensic DNA analysis a laboratory manual J. Thomas McClintock |
| title_fullStr | Forensic DNA analysis a laboratory manual J. Thomas McClintock |
| title_full_unstemmed | Forensic DNA analysis a laboratory manual J. Thomas McClintock |
| title_short | Forensic DNA analysis |
| title_sort | forensic dna analysis a laboratory manual |
| title_sub | a laboratory manual |
| topic | DNA Analysis Laboratory manuals DNA fingerprinting Laboratory manuals Forensic genetics Laboratory manuals DNA Fingerprinting Laboratory Manuals Forensic Medicine methods Laboratory Manuals Gerichtliche Chemie (DE-588)4202330-0 gnd Fingerprint-Verfahren (DE-588)4231579-7 gnd Methode (DE-588)4038971-6 gnd DNS (DE-588)4070512-2 gnd |
| topic_facet | DNA Analysis Laboratory manuals DNA fingerprinting Laboratory manuals Forensic genetics Laboratory manuals DNA Fingerprinting Laboratory Manuals Forensic Medicine methods Laboratory Manuals Gerichtliche Chemie Fingerprint-Verfahren Methode DNS |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017314457&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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