SUMO protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana
2009
|
| Schriftenreihe: | Methods in molecular biology
497 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 324 S. Ill., graph. Darst. |
| ISBN: | 9781934115800 9781597455664 |
Internformat
MARC
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| 020 | |a 9781597455664 |9 978-1-59745-566-4 | ||
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| 245 | 1 | 0 | |a SUMO protocols |c ed. by Helle D. Ulrich |
| 264 | 1 | |a New York, NY |b Humana |c 2009 | |
| 300 | |a X, 324 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 497 | |
| 490 | 0 | |a Springer-Protocols | |
| 650 | 2 | |a Petites protéines apparentées à ubiquitine | |
| 650 | 2 | |a Ubiquitines | |
| 650 | 7 | |a Ubiquitines |2 ram | |
| 650 | 4 | |a Small Ubiquitin-Related Modifier Proteins |x analysis | |
| 650 | 4 | |a Small Ubiquitin-Related Modifier Proteins |x physiology | |
| 650 | 4 | |a Ubiquitin | |
| 650 | 0 | 7 | |a Modifikation |g Biochemie |0 (DE-588)4307844-8 |2 gnd |9 rswk-swf |
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| 689 | 0 | |C b |5 DE-604 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-017186793 | ||
Datensatz im Suchindex
| _version_ | 1804138712658870272 |
|---|---|
| adam_text | Contents
Preface
..................................................................
v
Contributors.
......................................................... ix
Part I: Introduction
1.
The SUMO System: An Overview
.................................... 3
Helle D. Ulrich
Part II:
Identification
of SUMO Targets
2.
Identification of SUMO Target Proteins
by Quantitative Proteomks
......................................... 19
Jens S. Andersen, Ivan
Matic,
and Alfred CO.
Vertegaal
3.
Identification of SUMO-Conjugated Proteins
and their SUMO Attachment Sites
Using Proteomic Mass Spectrometry
.................................. 33
James A. WoMscbleyel
4.
Identification of SUMO Targets Through
In Vitro Expression Cloning
........................................ 51
Christian B. Gocke and Hongtao Yu
5.
Enhanced Detection of In Vivo SUMO Conjugation
by Ubc9 Fusion-Dependent Sumoylation (UFDS)
........................ 63
Rainer Niedenthal
6.
In Vivo Detection and Characterization
of Sumoylation Targets in Saccharomyces cerevisiae
........................ 81
Helle
D.
Ulrich
and
Adelina A. Davies
Part III: Identification of SUMO Interactors
7.
Identification ofSUMO-Interacting Proteins
by Yeast Two-Hybrid Analysis
....................................... 107
Mary B. Kroetz and Mark Hochstrasser
8.
Identification of SUMO-Binding Motifs by NMR
........................ 121
Candace S.
Seu
and Yuan Chen
Part IV: Functional Analysis of Slmoylatiox In Vivo
9.
Regulation of Transcription Factor Activity by SUMO Modification
.......... 141
Jian
Ouyanß,
Alvaro
Valin,
and Grace Gill
10.
Characterization of the Effects and Functions
of Sumoylation Through Rapamycin-Mediated Heterodimerization
........... 153
Shanshan Zhu and Michael J. Matunis
viii Contents
Part V: Biochemical Studies of SUMO Conjugation
11.
Purification of SUMO Conjugating Enzymes
and Kinetic Analysis of Substrate Conjugation
........................... 167
AH A. Yunus and Christopher D. Lima
12.
Performing In Vitro Sumoylation Reactions
Using
Recombinant
Enzymes
...................................... 187
Andreas Werner, Marie-Christine Moutty,
Ulrike Möller,
and
Frauke Melchior
13.
Preparation of Sumoylated Substrates for Biochemical Analysis
............. 201
Puck Knipscheer,
Helene Klug,
Titia
К.
Sixma, and Andrea Pichler
14.
Strategies for the Expression of SUMO-Modified
Target Proteins in Escherichia
coli
................................... 211
Hisato Saitoh, Junsuke Uwada, and Azusa Kawasaki
Part VI: Biochemical Studies of SUMO Deconjugation
15.
Preparation of SUMO Proteases and Kinetic
Analysis Using Endogenous Substrates
............................... 225
David
Reverter
and Christopher D. Lima
16.
An In Vitro FRET-Based Assay for the Analysis
of SUMO Conjugation and Isopeptidase Cleavage
....................... 241
Nicolas Stankovic-Valentin,
Lukasz
Kozaczkiewicz,
Katja Curth,
and
Frauke Melchior
17.
FRET-Based In Vitro Assays for the Analysis
of SUMO Protease Activities
....................................... 253
Michael H. Tatham and Ronald T. Hay
18.
Detection and Characterization of SUMO Protease
Activity Using a Sensitive Enzyme-Based Reporter Assay
.................. 269
Craig A. Leach, Xufan
Tian,
Michael R.
Mattern,
and Benjamin Nicholson
Part
VII:
Manipulation and Alternative Uses of the SUMO System
19.
Inhibition of the SUMO Pathway by Garni
............................ 285
Mariaelena
Pozzebon,
Chiara
V.
Segré,
and Susanna
Chiocca
20.
SUMO Fusion Technology for Enhanced Protein
Production in Prokaryotic and Eukaryotic Expression Systems
.............. 303
Index
................................................................. 319
Erratum
©
Springer Science+Business Media, LLC
2009
Publisher s
erratum
to:
SUMO Protocols
Helle
D.
Ulrich (Editor), 2009
The published table of contents has omitted the authors of Chapter
20,
Sumo Fusion Technology for Enhanced Protein Production in
Prokaryotic and Eukaryotic Expression Systems. The authors are:
Tadas
Panavas,
Carsten
Sanders, and Tauseef R. Butt.
The published index contains inaccurate page references. An
electronic version of the corrected index can be found at:
http://λvw^v.springer.corn/humana+press/biocherшstryЉook/978-l-
934115-80-0
|
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| id | DE-604.BV035382558 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:32:33Z |
| institution | BVB |
| isbn | 9781934115800 9781597455664 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017186793 |
| oclc_num | 233934292 |
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| owner_facet | DE-355 DE-BY-UBR DE-11 |
| physical | X, 324 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Humana |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer-Protocols |
| spelling | SUMO protocols ed. by Helle D. Ulrich New York, NY Humana 2009 X, 324 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 497 Springer-Protocols Petites protéines apparentées à ubiquitine Ubiquitines Ubiquitines ram Small Ubiquitin-Related Modifier Proteins analysis Small Ubiquitin-Related Modifier Proteins physiology Ubiquitin Modifikation Biochemie (DE-588)4307844-8 gnd rswk-swf Ubiquitin-ähnliche Proteine (DE-588)4526337-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ubiquitin-ähnliche Proteine (DE-588)4526337-1 s Modifikation Biochemie (DE-588)4307844-8 s b DE-604 Ulrich, Helle D. Sonstige oth Methods in molecular biology 497 (DE-604)BV035362695 497 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017186793&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | SUMO protocols Methods in molecular biology Petites protéines apparentées à ubiquitine Ubiquitines Ubiquitines ram Small Ubiquitin-Related Modifier Proteins analysis Small Ubiquitin-Related Modifier Proteins physiology Ubiquitin Modifikation Biochemie (DE-588)4307844-8 gnd Ubiquitin-ähnliche Proteine (DE-588)4526337-1 gnd |
| subject_GND | (DE-588)4307844-8 (DE-588)4526337-1 (DE-588)4143413-4 |
| title | SUMO protocols |
| title_auth | SUMO protocols |
| title_exact_search | SUMO protocols |
| title_full | SUMO protocols ed. by Helle D. Ulrich |
| title_fullStr | SUMO protocols ed. by Helle D. Ulrich |
| title_full_unstemmed | SUMO protocols ed. by Helle D. Ulrich |
| title_short | SUMO protocols |
| title_sort | sumo protocols |
| topic | Petites protéines apparentées à ubiquitine Ubiquitines Ubiquitines ram Small Ubiquitin-Related Modifier Proteins analysis Small Ubiquitin-Related Modifier Proteins physiology Ubiquitin Modifikation Biochemie (DE-588)4307844-8 gnd Ubiquitin-ähnliche Proteine (DE-588)4526337-1 gnd |
| topic_facet | Petites protéines apparentées à ubiquitine Ubiquitines Small Ubiquitin-Related Modifier Proteins analysis Small Ubiquitin-Related Modifier Proteins physiology Ubiquitin Modifikation Biochemie Ubiquitin-ähnliche Proteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017186793&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT ulrichhelled sumoprotocols |