Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Verl. Dr. Hut
2008
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Pharmazeutische Biologie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 195 S. Ill., graph. Darst. 21 cm, 331 gr. |
| ISBN: | 9783899639124 |
Internformat
MARC
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| 100 | 1 | |a Mehner, Christian Michael |d 1979- |e Verfasser |0 (DE-588)137613199 |4 aut | |
| 245 | 1 | 0 | |a Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare |c von Christian Michael Mehner |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a München |b Verl. Dr. Hut |c 2008 | |
| 300 | |a XII, 195 S. |b Ill., graph. Darst. |c 21 cm, 331 gr. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Pharmazeutische Biologie | |
| 502 | |a Zugl.: Bonn, Univ., Diss., 2008 | ||
| 650 | 4 | |a Blaualgen - Bioaktive Verbindungen | |
| 650 | 0 | 7 | |a Cyanobakterien |0 (DE-588)4136726-1 |2 gnd |9 rswk-swf |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017178952&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804138703728148480 |
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| adam_text | -TABLE OF CONTENTS- TABLE OF CONTENTS PAGE 1 INTRODUCTION 1 1 . 1 THE
BIOLOGY OF CYANOBACTERIA 1 1.2 CYANOBACTERIAL IMPACT ON HUMAN BEINGS:
TOXINS AND LEAD STRUCTURES 2 1.3 THE BIOSYNTHESIS OFPEPTIDES IN
CYANOBACTERIA 5 1.3.1 NON RIBOSOMAL PEPTIDE SYNTHETASES (NRPSS) 5 1.3.2
POLYKETIDE SYNTHASES (PKSS) 8 1.4 COMBINATORIAL CHEMISTRY IN
CYANOBACTERIA - THE PEPTIDES 9 1.4.1 CYANOPEPTOLINS 9 1.4.2 MICROCYSTINS
13 1.4.3 ANABAENOPEPTINS 15 1.4.4 MICRO VIRIDINS 17 1.4.5
CYCLAMIDES/CYANOBACTINS 19 1.4.6 CRYPTOPHYCINS 22 1.4.7 NOSTOPEPTOLIDES
24 1.4.8 NOSTOCYCLOPEPTIDES 26 1.5 SUMMARY - COMBINATORIAL CHEMISTRY IN
CYANOBACTERIA 27 2 ALM OF THE PRESENT STUDY 29 3 MATERIAL AND METHODS
(GENERAL PROCEDURES) 30 3.1 CYANOBACTERIAL STRAINS 30 3.1.1 ORIGINOF THE
CYANOBACTERIAL STRAINS 30 3.1.2 CULTIVATION OF CYANOBACTERIA 34 3.2
EXTRACTION OF LYOPHILIZED CYANOBACTERIAL BIOMASS 36 3.2.1 CYANOBACTERIA
FOR SCREENING PURPOSE 36 3.2.2 EXTRACTION OFLARGE SCALECULTUREOF
AFEJTOCMRA/ARE^O/) 36 3.3 BIOLOGICAL SCREENING 37 3.3.1 ANTIMICROBIAL
SCREENING 37 3.3.1.1 AGAR DIFFUSION ASSAY 37 3.3.1.2 BROTH
MICRO-DILUTION METHOD (TYCHONAMIDE A) 38 3.3.2 CYTOTOXICITY 38 3.3.2.1
CYTOTOXICITY TEST AGAINST HUMAN CANCER CELL LINES 38 I BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/992163552 DIGITALISIERT DURCH -TABLE OF
CONTENTS- 3.3.2.2 CLONOGENIC ASSAYS WITH HUMAN TUMOUR XENOGRAFTS AND 40
HEMATOPOIETIC STEM CELLS 3.3.3 TESTING OF ANTIPROTOZOAL ACTIVITY 41
3.3.4 ENZYME INHIBITION ASSAYS 43 3.3.4.1 PREPARATION OF THE CRUDE
EXTRACTS 43 3.3.4.2 HLE INHIBITION ASSAY 43 3.3.4.3 HLE INCUBATION
EXPERIMENT 44 3.3.4.4 CATHEPSIN G INHIBITION ASSAY 44 3.3.4.5 PROTEINASE
3 INHIBITION ASSAY 45 3.3.4.6 ACHE INHIBITION ASSAY 45 3.3.4.7 ENZYMATIC
ANALYSES 47 3.3.5 ANTIVIRAL ASSAY 47 3.3.6 P2Y-RECEPTOR ASSAY 49 1.4
MOLECULAR BIOLOGICAL METHODS 50 3.4.1 DNAISOLATION 50 3.4.2
DETERMINATION OF DNA CONCENTRATION AND PURITY 51 3.4.3 AMPLIFICATION OF
DNA USING POLYMERASE CHAIN REACTION (PCR) 51 3.4.3.1 ^-SCREENING 51
3.4.3.2 PFO-SCREENING 52 3.4.3.3 PCR AMPLIFICATION OF NOIF 53 3.4.4
AGAROSE-GEL ELECTROPHORESIS 53 3.4.5 DNA EXTRACTION FROM AGAROSE GELS 54
3.4.6 PREPARATION OF E. COLI COMPETENT CELLS (XL-1 BLUE) 54 3.4.7
CLONING OF PCR PRODUCTS IN E. COLI XL-1 BLUE CELLS 54 3.4.8 PREPARATION
OF PLASMIDS 55 3.4.9 AUTOMATED DNA SEQUENCING 55 5.5 CHROMATOGRAPHY 56
3.5.1 VACUUM LIQUID CHROMATOGRAPHY (VLC) 56 3.5.2 HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY (HPLC) 56 5.6 STRUCTURE ELUCIDATION 57 3.6.1 NMR
SPECTROSCOPY 57 3.6.2 MASS SPECTROMETRY 58 3.6. -TIIBLE OF CON(ENTS- 3.7
3.8 3.9 4 4.1 3.6.5 3.6.6 3.6.7 3.6.8 3.6.9 OPTICAL ROTATION CHIRAL
GC-MS CHIRAL HPLC ADVANCED MARFEY S METHOD OXIDATION OF INSULAPEPTOLIDE
A, B AND E SYNTHESIS OF O-METHYL-HOMOTYROSINE ENANTIOMERS MEDIA, PRIMER
BUFFERS AND SOLUTIONS RESULTS & DISCUSSION SCREENIINE STUDIES OF
CVANOBACTERIAL EXTRACTS 59 60 61 62 63 64 66 67 68 68 4.1.1
ANTIMICROBIAL TESTING 68 4.1.2 CYTOTOXICITY 68 4.1.3 ACHE AND HLE
INHIBITION ASSAYS 70 4.2 MOLECULAR BIOLOGICAL ANALYSES AND
CHARACTERIZATION OF THE BIOSYNTHETIC 71 POTENTIAL OF CYANOBACTERIAL
STRAINS 4.2.1 SCREENING FOR POLYKETIDE SYNTHASE (PKS) AND NONRIBOSOMAL
PEPTIDE 72 SYNTHETASE (NRPS) GENE FRAGMENTS 4.2.2 SPECIFICATION OF THE
BIOSYNTHETIC POTENTIAL OFNOSTOC INSULARE 73 4.2.3 MALDI-TOF
CHARACTERIZATION OF SYP ORANGE AND BO61BG-U 75 4.3 DISCUSSION AND
ASSESSMENT OF SCREENING RESULTS AND MOLECULAR BIOLOGICAL 76 ANALYSES 4.4
PEPTIDES OF TYCHONEMA SP. 79 4.4. -TABLE OF CONTCNTS- 4.5.3 BIOSYNTHETIC
CONSIDERATIONS AND STRUCTURAL DIVERSITY 99 4.5.4 BIOACTIVITY 101 4.5.5
ECOLOGICAL FUNCTION 102 4.6 INVESTIGATION OF THE STRAIN NOSTOC INSULARE
104 4.6.1 ISOLATION OFNEWCYANOPEPTOLINS 104 4.6.2 STRUCTURE ELUCIDATION
OF THE INSULAPEPTOLIDES 107 4.6.3 BIOLOGICAL ACTIVITY OF
INSULAPEPTOLIDES 119 4.7 DISCUSSION OF THE RESULTS CONCERNING THE
INSULAPEPTOLIDES 124 4.7.1 STRAIN NOSTOC INSULARE (NOSTOCALES) 124 4.7.2
STRUCTURAL FEATURES 125 4.7.3 BIOSYNTHETIC CONSIDERATIONS AND STRUCTURAL
DIVERSITY 126 4.7.4 BIOACTIVITY 132 4.7.5 ECOLOGICAL FUNCTION 135 5
SUMMARY 136 6 REFERENCES 139 7 APPENDIX 161 NMR SPECTRAL DATA 161
MOLECULAR BIOLOGICAL DATA 192 XN
|
| any_adam_object | 1 |
| author | Mehner, Christian Michael 1979- |
| author_GND | (DE-588)137613199 |
| author_facet | Mehner, Christian Michael 1979- |
| author_role | aut |
| author_sort | Mehner, Christian Michael 1979- |
| author_variant | c m m cm cmm |
| building | Verbundindex |
| bvnumber | BV035374616 |
| ctrlnum | (OCoLC)318215364 (DE-599)DNB992163552 |
| dewey-full | 579.390724 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579.390724 |
| dewey-search | 579.390724 |
| dewey-sort | 3579.390724 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV035374616 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:32:24Z |
| institution | BVB |
| isbn | 9783899639124 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017178952 |
| oclc_num | 318215364 |
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| owner_facet | DE-12 DE-19 DE-BY-UBM DE-29T |
| physical | XII, 195 S. Ill., graph. Darst. 21 cm, 331 gr. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Pharmazeutische Biologie |
| spelling | Mehner, Christian Michael 1979- Verfasser (DE-588)137613199 aut Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare von Christian Michael Mehner 1. Aufl. München Verl. Dr. Hut 2008 XII, 195 S. Ill., graph. Darst. 21 cm, 331 gr. txt rdacontent n rdamedia nc rdacarrier Pharmazeutische Biologie Zugl.: Bonn, Univ., Diss., 2008 Blaualgen - Bioaktive Verbindungen Cyanobakterien (DE-588)4136726-1 gnd rswk-swf Bioaktive Verbindungen (DE-588)4145589-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Cyanobakterien (DE-588)4136726-1 s Bioaktive Verbindungen (DE-588)4145589-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017178952&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mehner, Christian Michael 1979- Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare Blaualgen - Bioaktive Verbindungen Cyanobakterien (DE-588)4136726-1 gnd Bioaktive Verbindungen (DE-588)4145589-7 gnd |
| subject_GND | (DE-588)4136726-1 (DE-588)4145589-7 (DE-588)4113937-9 |
| title | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare |
| title_auth | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare |
| title_exact_search | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare |
| title_full | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare von Christian Michael Mehner |
| title_fullStr | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare von Christian Michael Mehner |
| title_full_unstemmed | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare von Christian Michael Mehner |
| title_short | Bioactive peptides from the cyanobacterial strains Tychonema sp. and Nostoc insulare |
| title_sort | bioactive peptides from the cyanobacterial strains tychonema sp and nostoc insulare |
| topic | Blaualgen - Bioaktive Verbindungen Cyanobakterien (DE-588)4136726-1 gnd Bioaktive Verbindungen (DE-588)4145589-7 gnd |
| topic_facet | Blaualgen - Bioaktive Verbindungen Cyanobakterien Bioaktive Verbindungen Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017178952&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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