Mitochondrial DNA: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Dordrecht [u.a.]
Humana Press
2009
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
554 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 403 S. Ill., graph. Darst. |
| ISBN: | 9781934115602 9781597455213 |
Internformat
MARC
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| 245 | 1 | 0 | |a Mitochondrial DNA |b methods and protocols |c ed. by Jeffrey A. Stuart |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Dordrecht [u.a.] |b Humana Press |c 2009 | |
| 300 | |a XI, 403 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 554 | |
| 650 | 4 | |a DNA, Mitochondrial |v Laboratory Manuals | |
| 650 | 4 | |a Genetic Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Mitochondrial DNA |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804138692001923072 |
|---|---|
| adam_text | Contents
Preface
............................................................
γ
Contributors
........................................................ ix
Part I: Mitochondrial
DNA
Replication, Transcription,
Translation, and Respiratory Complex Assembly
1.
Biochemical Isolation of mtDNA
Nucleoide
from Animal Cells
............... 3
Daniel F.
Bogenhagen
2.
Analysis of Mitochondrial
DNA
by Two-Dimensional
Agarose
Gel Electrophoresis
................................................. 15
Aurelio
Reyes, Takehiro Yasukawa,
Trida
J
.
Cluett, and Ian
J.
Holt
3.
Comparative Purification Strategies for
Drosophila
and Human Mitochondrial
DNA
Replication Proteins:
DNA Polymerase
γ
and Mitochondrial
Single-Stranded
DNA-
Binding Protein
.................................. 37
Marcos T.
Oliveira
and Laurie S. Kaguni
4.
Functional Analysis of Mutant Mitochondrial
DNA
Polymerase Proteins
Involved in Human Disease
.......................................... 59
Sherine S. L. Chan and William C. Copeland
5.
Preparation of Human Mitochondrial Single-Stranded
DNA-Binding Protein
............................................... 73
Matthew J. Longley, Leslie A. Smith, and William
С
Copeland
6.
Methods for Assessing Binding of Mitochondrial Transcription Factor A
(TFAM) to
DNA.................................................. 87
Atsushi Fukuoh and Dongchon Kang
7.
Inducible Expression in Human Cells, Purification, and In Vitro Assays
for the Mitochondrial
DNA Helicase
Twinkle
............................ 103
Steffi
Goff
art and Hans Spelbrink
8.
Purification Strategy for
Recombinant
Forms of the Human Mitochondrial
DNA Helicase.....................................................
121
Tawn
D.
Ziebarth and Laurie
S.
Kaguni
9.
Methods for Studying Mitochondrial Transcription Termination with
Isolated Components
............................................... 127
Paola Loguercio Pelosa,
Stefania
Deceglie,
Marina Roberti,
Maria Nicola
Gada leta,
and
Palmiro
Cantatore
10.
Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins
and Assembly of Individual Structural Subunits into Functional
Holoenzyme Complexes
............................................. 143
Scot C. Leary and Florin Sasarman
VII
viii Contents
Part II: Mitochondrial
DNA
Damage and Repair
11.
Reactive Oxygen Species Production by Mitochondria
...................... 165
Adrian J. Lambert and Martin D. Brand
12.
Measuring mtDNA Damage Using a Supercoiling-Sensitive
qPCRApproach
................................................... 183
Sam W. Chan andjunjian Z. Chen
13.
Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial
DNA
by Immunofluorescence Method
................................. 199
Mizuki Ohno, Sugako Oka, and Yusaku Nakabeppu
14.
In Vitro Measurement of
DNA
Base Excision Repair
in Isolated Mitochondria
............................................. 213
Melissa M. Page and Jeffrey A. Stuart
15.
Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through
Stable Transfection, Transient Transfection, Viral Transduction, and
TAT-Mediated Protein Transduction
................................... 233
Christopher A. Koczor, Janet W. Snyder,
Inna
N.
Shokolenko,
Allison W.
Dobson,
Glenn L. Wilson, and Susan P. LeDoux
16.
Construction and Characterization of a Cell Line Deficient in Repair of
Mitochondrial, but Not Nuclear, Oxidative
DNA
Damage
.................. 251
Sugako Oka, Mizuki Ohno, and Yusaku Nakabeppu
Part III: Mitochondrial
DNA Mutations
17.
Mitochondrial
DNA
Oxidative Damage and Mutagenesis in
Saccharomyces cerevisiae
.............................................. 267
Lyra M. Griffiths, Nicole A. Doudican, Gerald S. Shadel, and Paul W. Doetsch
18.
Determination of
DNA
Mutation Load in Human Tissues Using Denaturing
HPLC-Based Heteroduplex Analysis
................................... 287
Kok
Seong
Lim,
Robert
К.
Naviaux, and Richard H. Haas
19.
Rapid Identification of Unknown Heteroplasmic Mitochondrial
DNA
Mutations with Mismatch-Specific Surveyor Nuclease
...................... 301
Sylvie
Bannwarth, Vincent
Procaccio,
and
Véronique
Paquis-Flucklinger
20.
Collection of Isolated Cells for Studying Mitochondrial
DNA
Mutations
Within Individual Cells
.............................................. 315
Yevgenya Kraytsberg, Natalya Bodyak, Susan Myerow, Alexander Nicholas,
Konstantin
Ebralidze, and
Konstantin
Khrapko
21.
Quantitative Analysis of Somatic Mitochondrial
DNA
Mutations by Single-Cell
Single-Molecule PCR
............................................... 329
Yevgenya Kraytsberg, Natalya Bodyak, Susan Myerow, Alexander Nicholas,
Konstantin
Ebralidze, and
Konstantin
Khrapko
22.
Measuring
DNA
Precursor Pools in Mitochondria
......................... 371
Christopher K. Mathews and Linda J. Wheeler
23.
Establishment of Human Cell Lines Lacking Mitochondrial
DNA............. 383
Kazunari Hashiguchi and Qiu-Mei Zhang-Akiyama
Index
................................................................ 393
|
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| isbn | 9781934115602 9781597455213 |
| language | English |
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| series | Methods in molecular biology |
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| spelling | Mitochondrial DNA methods and protocols ed. by Jeffrey A. Stuart 2. ed. Dordrecht [u.a.] Humana Press 2009 XI, 403 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 554 DNA, Mitochondrial Laboratory Manuals Genetic Techniques Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS (DE-588)4202301-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Mitochondriale DNS (DE-588)4202301-4 s Methode (DE-588)4038971-6 s b DE-604 Stuart, Jeffrey A. Sonstige oth Methods in molecular biology 554 (DE-604)BV035362695 554 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017169826&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitochondrial DNA methods and protocols Methods in molecular biology DNA, Mitochondrial Laboratory Manuals Genetic Techniques Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS (DE-588)4202301-4 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4202301-4 (DE-588)4038971-6 |
| title | Mitochondrial DNA methods and protocols |
| title_auth | Mitochondrial DNA methods and protocols |
| title_exact_search | Mitochondrial DNA methods and protocols |
| title_full | Mitochondrial DNA methods and protocols ed. by Jeffrey A. Stuart |
| title_fullStr | Mitochondrial DNA methods and protocols ed. by Jeffrey A. Stuart |
| title_full_unstemmed | Mitochondrial DNA methods and protocols ed. by Jeffrey A. Stuart |
| title_short | Mitochondrial DNA |
| title_sort | mitochondrial dna methods and protocols |
| title_sub | methods and protocols |
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| topic_facet | DNA, Mitochondrial Laboratory Manuals Genetic Techniques Laboratory Manuals Mitochondrial DNA Laboratory manuals Mitochondriale DNS Methode |
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