Bacteriophages: methods and protocols 1 Isolation, characterization and interactions
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
| Schriftenreihe: | Methods in molecular biology
501 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXII, 307 S. Ill., graph. Darst. |
| ISBN: | 9781617377150 |
Internformat
MARC
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| 245 | 1 | 0 | |a Bacteriophages |b methods and protocols |n 1 |p Isolation, characterization and interactions |c ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2009 | |
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| 490 | 1 | |a Methods in molecular biology |v 501 | |
| 490 | 0 | |a Methods in molecular biology |v ... | |
| 650 | 4 | |a Bacteriophages | |
| 650 | 4 | |a Bacteriophages - Research - Methodology | |
| 650 | 4 | |a Bacteriophages - isolation & purification - Laboratory Manuals | |
| 650 | 4 | |a Bacteriophages - physiology - Laboratory Manuals | |
| 650 | 4 | |a Genomics - methods - Laboratory Manuals | |
| 700 | 1 | |a Clokie, Martha R. J. |e Sonstige |4 oth | |
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Datensatz im Suchindex
| _version_ | 1804138664044789760 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Introduction xiii
Section 1. Bacteriophage Isolation
1 Methods for the Isolation of Viruses from Environmental Samples
K. Eric Wommack, KurtE. Williamson, Rebekah R. Helton, Sbellie R. Bench,
and Danielle M. WinJet 3
2 Bacteriophage Enrichment from Water and Soil
Rohan Van Twest and Andrew M. Kropinski 15
3 Isolation of Phage via Induction of Lysogens
RaulR.Raya andElvira M. HSbert 23
4 Isolation of Cyanophages from Aquatic Environments
Andrew D. Millard 33
5 Isolation of Viruses from High Temperature Environments
Jennifer Fulton, Trevor Douglas, and Mark Young 43
6 Isolation of Novel Large and Aggregating Bacteriophages
Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Borries Demeler,
and Stephen C. Hardies 55
Section 2. Bacteriophage Characterization
7 Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
Andrew M. Kropinski, Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr
and Roger P. Johnson 69
8 Enumeration of Bacteriophages by the Direct Plating Plaque Assay
Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr, and Roger P. Johnson 77
9 Enumeration of Bacteriophages Using the Small Drop Plaque Assay
System
Amanda Mazzocco, Thomas E. Waddell, Erika Lingohr and Roger P. Johnson 81
10 Determination of Virus Abundance
by Epifluorescence Microscopy
Alice C. Ortmann and Curtis A. Suttle 87
11 Enumeration of Bacteriophages Using Flow Cytometry
Corina P. D. Brussaard 97
12 Basic Phage Electron Microscopy
Hans-W. Ackermann 113
vii
viii Contents
13 Phage Classification and Characterization
Hans-W. Ackermann 127
14 Phage Host Range and Efficiency of Plating
Elizabeth Kutter 141
15 Measurement of the Rate of Attachment
of Bacteriophage to Cells
Andrew M. Kropinski 151
16 Measurement of the Bacteriophage Inactivation Kinetics with Purified
Receptors
Andrew M. Kropinski 157
17 Bacteriophage Plaques: Theory and Analysis
Stephen T. Abedon and John Tin 161
18 Practical Methods for Determining Phage Growth Parameters
PaulHymanandStephen T. Abedon 175
19 Phage Production and Maintenance of Stocks, Including Expected Stock
Lifetimes
Louis-Charles Fortier and Sylvain Moineau 203
Section 3. Bacteriophage-Host Interactions
20 Construction of Phage Mutants
Robert Villafane 223
21 Modifying Bacteriophage with Recombineering
Lynn C. Thomttson, Amos B. Oppenheim, and Donald L. Court 239
22 Identification and Isolation of Lysogens with Induced Prophage
Jonathan Livny, Christopher N. LaRock, and David I. Friedman 253
23 Generalized Transduction
Anne Thierauf, Gerardo Perez, and Stanley Maloy 267
24 Preparation and Characterization of Anti-phage Serum
Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco,
and Roger P. Johnson 287
25 Generalized Transduction by Lytic Bacteriophages
Thomas E. Waddell, Kristyn Franklin, Amanda Mazzocco,
Andrew M. Kropinski and Roger P. Johnson 293
Index 305
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T21:31:47Z |
| institution | BVB |
| isbn | 9781617377150 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017149904 |
| oclc_num | 643726182 |
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| owner_facet | DE-12 DE-355 DE-BY-UBR DE-11 DE-188 DE-20 |
| physical | XXII, 307 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Bacteriophages methods and protocols 1 Isolation, characterization and interactions ed. by Martha R.J. Clokie and Andrew M. Kropinski Totowa, NJ Humana Press 2009 XXII, 307 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 501 Methods in molecular biology ... Bacteriophages Bacteriophages - Research - Methodology Bacteriophages - isolation & purification - Laboratory Manuals Bacteriophages - physiology - Laboratory Manuals Genomics - methods - Laboratory Manuals Clokie, Martha R. J. Sonstige oth (DE-604)BV035345676 1 Methods in molecular biology 501 (DE-604)BV035362695 501 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017149904&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bacteriophages methods and protocols Methods in molecular biology Bacteriophages Bacteriophages - Research - Methodology Bacteriophages - isolation & purification - Laboratory Manuals Bacteriophages - physiology - Laboratory Manuals Genomics - methods - Laboratory Manuals |
| title | Bacteriophages methods and protocols |
| title_auth | Bacteriophages methods and protocols |
| title_exact_search | Bacteriophages methods and protocols |
| title_full | Bacteriophages methods and protocols 1 Isolation, characterization and interactions ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_fullStr | Bacteriophages methods and protocols 1 Isolation, characterization and interactions ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_full_unstemmed | Bacteriophages methods and protocols 1 Isolation, characterization and interactions ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_short | Bacteriophages |
| title_sort | bacteriophages methods and protocols isolation characterization and interactions |
| title_sub | methods and protocols |
| topic | Bacteriophages Bacteriophages - Research - Methodology Bacteriophages - isolation & purification - Laboratory Manuals Bacteriophages - physiology - Laboratory Manuals Genomics - methods - Laboratory Manuals |
| topic_facet | Bacteriophages Bacteriophages - Research - Methodology Bacteriophages - isolation & purification - Laboratory Manuals Bacteriophages - physiology - Laboratory Manuals Genomics - methods - Laboratory Manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017149904&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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