Practical enzymology:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2007
|
| Ausgabe: | 1. ed., 1. reprint |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 255 S. graph. Darst. |
| ISBN: | 9783527304448 |
Internformat
MARC
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| 245 | 1 | 0 | |a Practical enzymology |c Hans Bisswanger |
| 250 | |a 1. ed., 1. reprint | ||
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2007 | |
| 300 | |a XV, 255 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Enzimler | |
| 650 | 4 | |a Enzimler - Analiz | |
| 650 | 4 | |a Enzimoloji | |
| 650 | 4 | |a Enzymes | |
| 650 | 4 | |a Enzymes |x Analysis | |
| 650 | 4 | |a Enzymology | |
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Datensatz im Suchindex
| _version_ | 1804138645741895680 |
|---|---|
| adam_text | Contents
Abbreviations
XIII
1
Introduction
1
2
Enzyme Nomenclature
5
3
Enzyme Reactions
7
3.1
Theory of the Enzyme Reaction
7
3.1.1
Reaction Order
7
3.1.1.1
Zero-order Reactions
8
3.1.1.2
First-order Reactions
8
3.1.1.3
Second- and Higher-order Reactions
9
3.2
Michaelis-Menten Equation
10
3.3
Theory of the Enzyme Assay
16
3.3.1
Analysis of Progress Curves
16
3.3.2
How To Develop an Enzyme Assay
21
3.3.2.1
General Considerations
21
3.3.2.2 pH 22
3.3.2.3
Ionic Strength and Buffer
23
3.3.2.4
Temperature
23
3.3.2.5
Special Components
24
3.3.2.6
Concentration of Components and Technical Implications
26
3.3.3
Enzyme Activity
28
3.3.3.1
Definition of Enzyme Units
28
3.3.3.2
Absorption Coefficient
28
3.3.3.3
Calculation of Enzyme Activity
29
3.4
Theory of Coupled Enzyme Reactions
32
3.4.1
Two Coupled Reactions
32
3.4.2
Three Coupled Enzyme Reactions
35
3.5
Substrate Determination
35
3.5.1
End-point Method
35
3.5.2
Coupled Enzyme Reactions
37
3.5.3
Kinetic Method for Substrate Determination
38
Practical Enz^mology. H. Bisswanger
Copyright
ê
2004
WILEY-VCH
Verlag
GmbH
&
Co. KGaA,
Weinheim
ISBN
3-527-30444-4
VIII Contents
3.5.4
Enzymatic Cycling
38
3.6
Instrumental Aspects
40
3.6.1
Spectroscopie
Methods
41
3.6.1.1
UV/Visible Photometry
41
3.6.1.2
Turbidity Measurements
46
3.6.1.3
Fluorimeter
47
3.6.1.4
Luminometry
50
3.6.1.5
Polarimetry
51
3.6.2
Electrochemical Measurements
52
3.6.2.1 pH
Meter
52
3.6.2.2
pH Stat
53
3.6.2.3
Potentiometry
54
3.6.3
Analysis of Reactions that Release or Consume Gas
54
3.6.3.1
Warburg Manometer
54
3.6.3.2
Radioactive Labeling
55
3.6.3.3
Oxygen and Carbon Dioxide Electrodes
55
3.7
Enzyme Assays
56
3.7.1
General Considerations
56
3.7.2
Practical Aspects
59
3.7.3
Buffers and Solutions
59
3.7.3.1
Theoretical Aspects
59
3.7.3.2
Preparation of Buffers
63
3.7.3.3
Frequently Used Buffers and Solutions
61
3.7.3.4
Calculation of Enzyme Activities
62
3.7.4
Oxidoreductase Assays
64
3.7.4.1
Optical Assays
64
3.7.4.2
Fluorimetrie
Assays
65
3.7.4.3
Alcohol Dehydrogenase
66
3.7.4.4
Shikimate Dehydrogenase
68
3.7.4.5
L-Lactate Dehydrogenase
68
3.7.4.6
Isocitrate
Dehydrogenase
71
3.7.4.7
Glucose-
6
-phosphate Dehydrogenase
72
3.7.4.8
Malate
Dehydrogenase
72
3.7.4.9
Glucose
Oxidase
73
3.7.4.10
Glyceraldehyde-S -phosphate Dehydrogenase
74
3.7.4.11
Pyruvate Ferredoxin Oxidoreductase
76
3.7.4.12
Glutamate
Dehydrogenase
76
3.7.4.13
L-
Amino
Acid
Oxidase
77
3.7.4.14
Uncase
78
3.7.4.15
Catalase
79
3.7.4.16
Peroxidase
80
3.7.4.17
Luciferase
82
3.7.5
Pyruvate Dehydrogenase Complex (PDHC)
83
3.7.5.1
Overall Activity of PDHC by NAD+ Reduction
84
3.7.5.2
Overall Activity of PDHC by
Dismutation
Assay
85
Contents
IX
3.7.5.3
Pyruvate Dehydrogenase (Lipoamide)
86
3.7.5.4
Dihydrolipoamide Acetyltransferase
88
3.7.5.5
Dihydrolipoamide Dehydrogenase
90
3.7.6
α
-Oxoglutarate
Dehydrogenase Complex (OGDHC)
91
3.7.6.1
Overall
Activity by NAD+ Reduction
92
3.7.6.2
α
-Oxoglutarate
Dehydrogenase
93
3.7.7
Transferases
94
3.7.7.1
Fatty Acid
Synthase
94
3.7.7.2
Phosphorylase a
95
3.7.7.3 Hexokinase 96
3.7.7.4
Pyruvate Kinase
97
3.7.7.5
Acetate
Kinase
98
3.7.7.6
3-Phosphoglycerate Kinase
99
3.7.8
Hydrolases
300
3.7.8.1
Lipase
100
3.7.8.2
Cholinesterase
102
3.7.8.3
Acetylcholinesterase
104
3.7.8.4
Alkaline Phosphatase
305
3.7.8.5
Acid Phosphatase
306
3.7.8.6
Ribonuclease
(Pancreatic)
107
3.7.8.7
α
-Amylase
І08
3.7.8.8
Amyloglucosidase
109
3.7.8.9
Lysozyme
309
3.7.8.10
a-Glucosidase
110
3.7.8.11 ß-Galactosidase 112
3.7.8.12 ß-Fructosidase 113
3.7.9
Proteases
3 33
3.7.9.1
Anson
Assay
114
3.7.9.2
Casein Assay
3 36
3.7.9.3
Azocasein Assay
117
3.7.9.4
Ninhydrin
Assay
117
3.7.9.5
Leucine Aminopeptidase
3 39
3.7.9.6
Chymotrypsin
3 39
3.7.9.7
Pepsin
121
3.7.9.8
Trypsin
122
3.7.9.9
Asparaginase
322
3.7.9.10
Urease
323
3.7.9.11
Adenosine Triphosphatase
325
3.7.10
Lyases
326
3.7.10.1
Pyruvate decarboxylase
326
3.7.10.2
Aldolase
127
3.7.10.3
Anthranilate Synthase
127
3.7.10.4
Carbonic Anhydrase
128
3.7.10.5
Fumarase
329
3.7.11
Isomerases
130
Χ Ι
Contents
3.7.11.1
Glucose/Xylose isomerase
230
3.7.12
Determination of Nicotinamide Nucleotides by Enzymatic Cycling
133
3.7.12.1
Determination of NADP(H)
133
3.7.12.2
Determination of NAD(H)
235
3.8
Diverse Methods
136
3.8.1
Protein Determination
136
3.8.1.1
Biuret Method
136
3.8.1.2
BCA Assay
137
3.8.1.3
Lowry Assay
139
3.8.1.4
Coomassie Binding Assay (Bradford Assay)
140
3.8.1.5
Absorption Method
141
3.8.1.6
Fluorimetrie
Assay
145
3.8.1.7
Ninhydrin
Assay
146
3.8.1.8
Modified
Ninhydrin
Assay for Protein Determination without
Hydrolysis
147
3.8.1.9
Protein Determination with 2-Hydroxy-l-naphthaldehyde
148
3.8.2
Phosphate Determination
149
3.8.3
Concentration of Enzyme Solutions
149
3.8.3.1
Precipitation
150
3.8.3.2 Ultrafiltration
and Dialysis
153
3.8.3.3
Ultracenrrifugation
154
3.8.3.4
Lyophilization
154
3.8.3.5
Miscellaneous Techniques
155
3.9
Enzyme Immunoassays
156
3.9.1
Non-competitive Solid-phase Enzyme Immunoassay
156
3.9.2
Competitive, Solid-phase Enzyme Immunoassay
157
3.9.3
Methods for Enzyme Immunoassays and Immobilization
Techniques
157
3.9.3.1
Protein Coupling to Cyanogen Bromide-activated
Agarose
157
3.9.3.2
Coupling of Diaminohexyl Spacer
159
3.9.3.3
Periodate
Activation of Cellulose
159
3.9.3.4
Conjugation of Proteins (Antibodies) to Enzymes (Peroxidase)
160
3.9.3.5
Introduction of Thiol Groups into Antibodies or Proteins
161
3.9.3.6
Conjugation of
ß-galactosidase
to Antibodies by MBS
161
3.9.3.7
Conjugation of Alkaline Phosphatase to Antibodies by
Glutaraldehyde
162
3.9.3.8
Cross-linking of Proteins with Dimethyl Suberimidate
162
4
Binding Measurements
165
4.1
Irreversible, Reversible, Specific, and Unspecific Binding
165
4.1.1
General Considerations
165
4.1.2
Experimental Aspects
167
4.1.2.1
Amount of Enzyme or
Macromolecule
168
4.2.1.2
Stability of the Enzyme or
Macromolecule
268
4.1.2.3
Stability of the Ligand
168
Contents
І
XI
4.1.2.4
Concentration of Components
269
4.2
Binding Measurement by Size Discrimination
170
4.2.1 Ultrafiltration 170
4.2.2
Equilibrium Dialysis
171
4.2.2.1
Determination of
Indole
Binding to Bovine Serum Albumin
172
4.2.3
Evaluation of Binding Experiments
275
4.2.4
Gel Filtration
177
4.2.5
Ultracentrifugation
179
4.3
Spectroscopic Techniques
180
4.3.1
Difference Spectroscopy
181
4.3.1.1
Difference Spectroscopic Determination of Binding of Ligands to
Catalase
283
4.3.1.2
Evaluation of Spectroscopic Binding Curves
288
4.3.2
Fluorescence Spectroscopy
289
4.3.2.1 ANS
Binding to Bovine Serum Albumin
292
4.4
Other Methods for Binding Measurements
295
4.4.1
Radioactive Labeling
295
4.4.2
Reflectrometric Interference Spectroscopy
296
5
Enzymes in Technical Applications
297
5.1
Principles of Enzyme Immobilization
298
5.1.1
Adsorption
299
5.1.2
Entrapment
299
5.1.3
Encapsulation
200
5.1.4
Cross-linking
202
5.1.5
Covalent Binding to Solid Supports
202
5.1.5.1
Supports
202
5.1.5.2
Spacer
203
5.2
Methods for Enzyme Immobilization
204
5.2.1
Microencapsulation inside Nylon Beads
205
5.2.2
Entrapment in Polyacrylamide
205
5.2.3
Covalent Immobilization of Enzymes on Nonporous Glass Surfaces
207
5.2.4
Immobilization on Controlled-pore Glass
209
5.2.5
Covalent Immobilization of Enzymes to
Polyamide 22 2
5.2.6
O-Alkylation with Triethyloxoniumtetrafluoroborate
222
5.2.7
Immobilization of Enzymes to
Amine
Groups after Partial Hydrolysis of
Polyamide 226
5.2.8
Immobilization of Enzymes to Carboxyl Groups after Partial Hydrolysis
of
Polyamide 227
5.2.9
Immobilization of Enzymes to Polyester
228
5.2.10
Immobilization by Alkaline Hydrolysis and Activation with
Tosylchloride
220
5.2.11
Alkaline Hydrolysis and Activation by Carbonyldiimidazol
222
5.3
Analysis of Immobilized Enzymes
222
5.3.1
Protein Assays
222
XII Contents
5.3.2 Enzyme
Assays
222
5.3.2.1
Modified Optical Assay for Immobilized Enzymes
223
5.3.2.2
Cofactors in Reactions with Immobilized Enzymes
224
5.4
Enzyme Reactors
225
5.4.1
Batch Reactor (Stirred-tank Reactor)
226
5.4.2
Membrane Reactor
226
5.4.3
Solid-bed Reactor
227
5.4.4
Immobilized Cells
227
5.5
Biosensors
228
5.5.1
Enzyme Electrodes
228
5.5.2
Immunoelectrodes
232
5.5.3
Other Biosensors
233
5.5.4
Bioaffinity Sensors
233
5.6
Immobilized Enzymes in Therapy
234
Index
249
|
| any_adam_object | 1 |
| author | Bisswanger, Hans 1943- |
| author_GND | (DE-588)129060461 |
| author_facet | Bisswanger, Hans 1943- |
| author_role | aut |
| author_sort | Bisswanger, Hans 1943- |
| author_variant | h b hb |
| building | Verbundindex |
| bvnumber | BV035332826 |
| callnumber-first | Q - Science |
| callnumber-label | QP601 |
| callnumber-raw | QP601 |
| callnumber-search | QP601 |
| callnumber-sort | QP 3601 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WD 5050 |
| classification_tum | CHE 828f |
| ctrlnum | (OCoLC)212595177 (DE-599)BSZ278643272 |
| discipline | Biologie Chemie |
| edition | 1. ed., 1. reprint |
| format | Book |
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| genre_facet | Praktikum Lehrbuch |
| id | DE-604.BV035332826 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:31:29Z |
| institution | BVB |
| isbn | 9783527304448 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017137243 |
| oclc_num | 212595177 |
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| owner_facet | DE-703 DE-91S DE-BY-TUM |
| physical | XV, 255 S. graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Bisswanger, Hans 1943- Verfasser (DE-588)129060461 aut Practical enzymology Hans Bisswanger 1. ed., 1. reprint Weinheim Wiley-VCH 2007 XV, 255 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Enzimler Enzimler - Analiz Enzimoloji Enzymes Enzymes Analysis Enzymology Enzymkinetik (DE-588)4133166-7 gnd rswk-swf Enzymtechnologie (DE-588)4135962-8 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf 1\p (DE-588)4127380-1 Praktikum gnd-content 2\p (DE-588)4123623-3 Lehrbuch gnd-content Enzym (DE-588)4014988-2 s DE-604 Enzymtechnologie (DE-588)4135962-8 s Enzymkinetik (DE-588)4133166-7 s 3\p DE-604 Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017137243&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Bisswanger, Hans 1943- Practical enzymology Enzimler Enzimler - Analiz Enzimoloji Enzymes Enzymes Analysis Enzymology Enzymkinetik (DE-588)4133166-7 gnd Enzymtechnologie (DE-588)4135962-8 gnd Enzym (DE-588)4014988-2 gnd |
| subject_GND | (DE-588)4133166-7 (DE-588)4135962-8 (DE-588)4014988-2 (DE-588)4127380-1 (DE-588)4123623-3 |
| title | Practical enzymology |
| title_auth | Practical enzymology |
| title_exact_search | Practical enzymology |
| title_full | Practical enzymology Hans Bisswanger |
| title_fullStr | Practical enzymology Hans Bisswanger |
| title_full_unstemmed | Practical enzymology Hans Bisswanger |
| title_short | Practical enzymology |
| title_sort | practical enzymology |
| topic | Enzimler Enzimler - Analiz Enzimoloji Enzymes Enzymes Analysis Enzymology Enzymkinetik (DE-588)4133166-7 gnd Enzymtechnologie (DE-588)4135962-8 gnd Enzym (DE-588)4014988-2 gnd |
| topic_facet | Enzimler Enzimler - Analiz Enzimoloji Enzymes Enzymes Analysis Enzymology Enzymkinetik Enzymtechnologie Enzym Praktikum Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017137243&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bisswangerhans practicalenzymology |