Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
DVG
2008
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| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 97 S. Ill., graph. Darst. 21 cm |
| ISBN: | 9783939902874 |
Internformat
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Datensatz im Suchindex
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| adam_text | MOLECULAR PATHOGENESIS, DIFFERENTIAL TRANSCRIPTION OF ENZYMES FORMING
THE LYSOSOMAL MULTIENZYMIC COMPLEX AND MICROSATELLITE BASED GENOTYPING
IN CANINE G M RGANGLIOSIDOSIS THESIS SUBMITTED IN PARTIAL FULFILLMENT OF
THE REQUIREMENTS FOR THE DEGREE DOCTOR OF PHILOSOPHY - PH.D.- AT THE
DEPARTMENT OF PATHOLOGY UNIVERSITY OF VETERINARY MEDICINE HANNOVER AND
THE CENTER FORISYSTEMSNEUROSCIERICE HANNOVER AWARDED BY THE UNIVERSITY
OF VETERINARY MEDICINE HANNOVER BY DR. ROBERT KREUTZER BORN IN BRA$OV /
ROMANIA HANNOVER, GERMANY 2008 TABLE OF CONTENT CHAPTER 1 - GENERAL
INTRODUCTION 1 1.1. GMI -GANGLIOSIDOSIS 2 1.2. CAUSE OF
GMI-GANGLIOSIDOSIS 3 1.3. CLINICAL FEATURES AND CHARACTERISTICS OF GMI
-GANGLIOSIDOSIS 5 1.3.1. HUMAN GMRGANGLIOSIDOSIS 5 1.3.2.
GMI-GANGLIOSIDOSIS IN ANIMAL SPECIES 6 1.3.2.1. GMI-GANGLIOSIDOSIS IN
DOGS 6 1.3.2.2. GMI-GANGLIOSIDOSIS IN CATS 8 1.3.2.3. GMI-GANGLIOSIDOSIS
IN CATTLE AND SHEEP 8 1.3.2.4. GMI-GANGLIOSIDOSIS IN BIRDS 8 1.4.
PATHOPHYSIOLOGY OF GMI-GANGLIOSIDOSIS 9 1.4.1. GMI-GANGLIOSIDE AND
BETA-GALACTOSIDASE 9 1.4.1.1. STRUCTURE AND LOCALIZATION OF
GMI-GANGLIOSIDE 9 1.4.1.2. FUNCTION OF GMI-GANGLIOSIDE 10 1.4.1.3.
BETA-GALACTOSIDASE BIOSYNTHESIS AND PROCESSING 11 1.4.1.4. DEGRADATION
PATHWAY OF GMI-GANGLIOSIDE 12 1.4.2. PATHOGENESIS OF GMI-GANGLIOSIDOSIS
13 1.4.2.1. EFFECTS OF GLBL GENETIC MODIFICATIONS ON ENZYMATIC ACTIVITY
13 1.4.2.2. MOLECULAR MECHANISMS OF CELLULAR DYSFUNCTION IN
GMI-GANGLIOSIDOSIS 14 1.4.3. BIOCHEMICAL AND PATHOLOGICAL FEATURES OF G
M I-GANGLIOSIDOSIS 16 1.4.3.1. HUMAN GMI-GANGLIOSIDOSIS 16 1.4.3.2.
ANIMAL GMI-GANGLIOSIDOSES 16 1.5. MOLECULAR DIAGNOSIS OF
GMI-GANGLIOSIDOSIS 18 1.6. REFERENCES 19 1.7. HYPOTHESES, AIMS AND
OUTLINE OF THE THESIS 33 1.7.1. HYPOTHESES 33 1.7.2. AIMS 33 1.7.3.
OUTLINE 34 CHAPTER 2 - GENOTYPE CONDITIONED DIFFERENTIAL TRANSCRIPTION
OF CANINE GLBL, CANINE NEUI AND PPCA IN AN ANIMAL MODEL OF LATE
INFANTILE GMI-GANGLIOSIDOSIS 35 ABSTRACT 36 MATERIALS AND METHODS 38
CELL CULTURE PROCEDURES 38 IV RNA EXTRACTION, CDNA SYNTHESIS AND PRIMER
DESIGN 38 QUANTITATIVE RT-PCR (QRT-PCR) 40 RESULTS 41 ACKNOWLEDGEMENT 49
REFERENCES 49 CHAPTER 3 - INSIGHTS INTO POST-TRANSLATIONAL PROCESSING OF
IJ-GALACTOSIDASE IN AN ANI- MAL MODEL RESEMBLING LATE INFANTILE HUMAN
GMI -GANGLIOSIDOSIS 54 ABSTRACT 55 INTRODUCTION 55 MATERIALS AND METHODS
57 COMPUTER-AIDED ANALYSES 57 RNA ISOLATION AND CDNA SYNTHESIS 57
EXPRESSION VECTOR CONSTRUCTION 57 CELL CULTURE PROCEDURES AND
TRANSFECTION 59 PROTEIN EXTRACTIONS 59 LYSOSOMES STAINING AND IMAGING 60
RESULTS 61 PREDICTED CHARACTERISTICS OF CANINE COMPARED TO HUMAN GLBL 61
INTRACELLULAR LOCALIZATION, PROCESSING AND ENZYMATIC ACTIVITY OF WILD
TYPE AND MUTANT CANINE GLB1 62 DISCUSSION 67 ACKNOWLEDGEMENTS 70
REFERENCES 70 CHAPTER 4 - RAPID AND ACCURATE GUII-GANGLIOSIDOSIS
DIAGNOSIS USING A PARENTAGE TESTING MICROSATELLITE 76 ABSTRACT 77 1.
INTRODUCTION 77 2. MATERIALS AND METHODS 78 2.1. DNA ISOLATION 78 2.2.
MICROSATELLITE AMPLIFICATION AND ANALYSIS 78 3. RESULTS . 79 4.
DISCUSSION AND CONCLUSIONS 79 ACKNOWLEDGEMENTS 80 REFERENCES 80 CHAPTER
5 - GENERAL DISCUSSIONS 83 5.1. GENOTYPE RELATED GLBL, NEUI AND PPCA
MRNA EXPRESSION 84 5.2. STRUCTURAL FEATURES, LYSOSOMAL TRANSPORT AND
MATURATION OF CANINE GLBL 85 5.3. GENOTYPE IDENTIFICATION BY
MICROSATELLITE ANALYSIS 88 5.4. REFERENCES 89 SUMMARY 94 ZUSAMMENFASSUNG
96 VI
|
| any_adam_object | 1 |
| author | Kreutzer, Robert 1972- |
| author_GND | (DE-588)130279390 |
| author_facet | Kreutzer, Robert 1972- |
| author_role | aut |
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| author_variant | r k rk |
| building | Verbundindex |
| bvnumber | BV035332744 |
| ctrlnum | (OCoLC)301991876 (DE-599)DNB991744543 |
| dewey-full | 636.70896858845043 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.70896858845043 |
| dewey-search | 636.70896858845043 |
| dewey-sort | 3636.70896858845043 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-07-09T21:31:29Z |
| institution | BVB |
| isbn | 9783939902874 |
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| spelling | Kreutzer, Robert 1972- Verfasser (DE-588)130279390 aut Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis by Robert Kreutzer 1. Aufl. Gießen DVG 2008 IX, 97 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: Hannover, Tierärztliche Hochsch., Diss., 2008 dogs cabt pathogenesis cabt transcription cabt enzymes cabt lysosomes cabt genotypes cabt gangliosidosis cabt autosomal-rezessiv cabt animal models cabt storage disorders cabt nervous system diseases cabt Erbkrankheit swd Galactosidase <beta-> swd Gangliosidose swd Husky swd RNS-Spleißen swd Typisierung swd (DE-588)4113937-9 Hochschulschrift gnd-content HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017137159&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kreutzer, Robert 1972- Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis dogs cabt pathogenesis cabt transcription cabt enzymes cabt lysosomes cabt genotypes cabt gangliosidosis cabt autosomal-rezessiv cabt animal models cabt storage disorders cabt nervous system diseases cabt Erbkrankheit swd Galactosidase <beta-> swd Gangliosidose swd Husky swd RNS-Spleißen swd Typisierung swd |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis |
| title_auth | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis |
| title_exact_search | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis |
| title_full | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis by Robert Kreutzer |
| title_fullStr | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis by Robert Kreutzer |
| title_full_unstemmed | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis by Robert Kreutzer |
| title_short | Molecular pathogenesis, differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine GM1-gangliosidosis |
| title_sort | molecular pathogenesis differential transcription of enzymes forming the lysosomal multienzymic complex and microsatellite based genotyping in canine gm1 gangliosidosis |
| topic | dogs cabt pathogenesis cabt transcription cabt enzymes cabt lysosomes cabt genotypes cabt gangliosidosis cabt autosomal-rezessiv cabt animal models cabt storage disorders cabt nervous system diseases cabt Erbkrankheit swd Galactosidase <beta-> swd Gangliosidose swd Husky swd RNS-Spleißen swd Typisierung swd |
| topic_facet | dogs pathogenesis transcription enzymes lysosomes genotypes gangliosidosis autosomal-rezessiv animal models storage disorders nervous system diseases Erbkrankheit Galactosidase <beta-> Gangliosidose Husky RNS-Spleißen Typisierung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017137159&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kreutzerrobert molecularpathogenesisdifferentialtranscriptionofenzymesformingthelysosomalmultienzymiccomplexandmicrosatellitebasedgenotypingincaninegm1gangliosidosis |