Apoptotic detection methods - from morphology to gene:
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Jena
Urban & Fischer
2003
|
| Ausgabe: | [Bindeeinh.] |
| Schriftenreihe: | Progress in histochemistry and cytochemistry
38,3 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | S. 278 - 339 Ill., graph. Darst. |
Internformat
MARC
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-017105166 | ||
Datensatz im Suchindex
| _version_ | 1804138600310243328 |
|---|---|
| adam_text | Contents
1 Introduction Y. Otsuki 283
2 History of cell death study Y. Otsuki 284
2.1 Overview 284
2.2 Apoptosis and necrosis 285
2.3 Apoptosis and oncosis 286
2.4 Apoptosis and programmed cell death 287
3 Apoptosis-related factors and its signaling Y. Otsuki 287
3.1 Bcl-2 family 287
3.2 TNF and TNF receptor family 288
3.3 Apoptotic signaling 289
4 Methods of morphological detection of apoptosis Y. Otsuki 289
4.1 Transmission electron microscopy 289
4.2 Histochemical dye staining 292
4.2.1 Hematoxylin and eosin staining 292
4.2.2 Toluidine blue staining of epoxy-resin-embedded sections 292
4.3 Fluorescence dye staining 293
4.4 Immunohistochemistry 294
4.4.1 Expression of Bcl-2 294
4.4.2 Expression of Fas and Fas ligand 295
4.5 Immunoelectron microscopy 296
4.5.1 Subcellular localization of Bcl-2 296
4.5.2 Subcellular localization of Fas and Fas ligand 296
4.5.3 Specificity of antibodies 297
4.6 TUNEL staining 299
4.6.1 Light microscopy 299
4.6.2 Transmission electron microscopy 300
4.6.2.1 Quantitative analysis of free 3 -OH DNA ends 301
4.6.2.2 Quantitative analysis of free 5 -OH DNA ends 303
4.6.3 Confocal laser microscopy 305
5 Flow cytometry M-A. Shibata 305
6 Biochemical methods for detecting apoptosis M-A. Shibata 308
6.1 Caspase activity 308
6.1.1 Colorimetric assay 308
6.1.2 Fluorometric assay 309
7 Molecular biological methods for detecting apoptosis 312
7.1 DNA agarose gel electrophoresis M-A. Shibata 312
7.2 Western blotting M-A. Shibata 314
7.3 RT-PCR M-A. Shibata 314
7.4 Transcriptional regulation of bcl-2 by nuclear proteins Z. Li 316
278 • Contents
7.4.1 Transcriptional factors of bcl-2 Z. Li 316
7.4.2 Transcriptional factors of bcl-2 in human endometrium Z. Li 321
7.4.3 Southwestern technique using BIACORE system Z. Li 321
8 Gene transfer for apoptotic induction M-A. Shibata 326
9 Concluding remarks Y. OtsukI 329
10 References 331
|
| any_adam_object | 1 |
| author | Ōtsuki, Yoshinori Li, Zhonglian Shibata, Masa-Aki |
| author_facet | Ōtsuki, Yoshinori Li, Zhonglian Shibata, Masa-Aki |
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| building | Verbundindex |
| bvnumber | BV035300288 |
| ctrlnum | (OCoLC)248981733 (DE-599)GBV363052542 |
| edition | [Bindeeinh.] |
| format | Book |
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| id | DE-604.BV035300288 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:30:46Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017105166 |
| oclc_num | 248981733 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-578 |
| owner_facet | DE-91 DE-BY-TUM DE-578 |
| physical | S. 278 - 339 Ill., graph. Darst. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Urban & Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Ōtsuki, Yoshinori Verfasser aut Apoptotic detection methods - from morphology to gene Yoshinori Otsuki ; Zhonglian Li ; Masa-Aki Shibata [Bindeeinh.] Jena Urban & Fischer 2003 S. 278 - 339 Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 38,3 Apoptosis Apoptosis (DE-588)4291096-1 gnd rswk-swf Apoptosis (DE-588)4291096-1 s DE-604 Li, Zhonglian Verfasser aut Shibata, Masa-Aki Verfasser aut Progress in histochemistry and cytochemistry 38,3 (DE-604)BV000005277 38,3 DE-605 pdf/application http://www.gbv.de/dms/hbz/toc/ht013664325.pdf 2008-11-15 Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017105166&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ōtsuki, Yoshinori Li, Zhonglian Shibata, Masa-Aki Apoptotic detection methods - from morphology to gene Progress in histochemistry and cytochemistry Apoptosis Apoptosis (DE-588)4291096-1 gnd |
| subject_GND | (DE-588)4291096-1 |
| title | Apoptotic detection methods - from morphology to gene |
| title_auth | Apoptotic detection methods - from morphology to gene |
| title_exact_search | Apoptotic detection methods - from morphology to gene |
| title_full | Apoptotic detection methods - from morphology to gene Yoshinori Otsuki ; Zhonglian Li ; Masa-Aki Shibata |
| title_fullStr | Apoptotic detection methods - from morphology to gene Yoshinori Otsuki ; Zhonglian Li ; Masa-Aki Shibata |
| title_full_unstemmed | Apoptotic detection methods - from morphology to gene Yoshinori Otsuki ; Zhonglian Li ; Masa-Aki Shibata |
| title_short | Apoptotic detection methods - from morphology to gene |
| title_sort | apoptotic detection methods from morphology to gene |
| topic | Apoptosis Apoptosis (DE-588)4291096-1 gnd |
| topic_facet | Apoptosis |
| url | http://www.gbv.de/dms/hbz/toc/ht013664325.pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017105166&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
| work_keys_str_mv | AT otsukiyoshinori apoptoticdetectionmethodsfrommorphologytogene AT lizhonglian apoptoticdetectionmethodsfrommorphologytogene AT shibatamasaaki apoptoticdetectionmethodsfrommorphologytogene |
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