Amplification methods for the immunolocalization of rare molecules in cells and tissues: with 9 tables
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Jena
Urban & Fischer
2001
|
| Ausgabe: | [Bindeeinh.] |
| Schriftenreihe: | Progress in histochemistry and cytochemistry
36,1 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 85 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV035294941 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 090206s2001 ad|| |||| 00||| eng d | ||
| 035 | |a (OCoLC)634969850 | ||
| 035 | |a (DE-599)GBV323920012 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91 |a DE-578 | ||
| 100 | 1 | |a Mayer, Gaétan |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Amplification methods for the immunolocalization of rare molecules in cells and tissues |b with 9 tables |c Gaétan Mayer ; Moı̈se Bendayan |
| 250 | |a [Bindeeinh.] | ||
| 264 | 1 | |a Jena |b Urban & Fischer |c 2001 | |
| 300 | |a 85 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Progress in histochemistry and cytochemistry |v 36,1 | |
| 700 | 1 | |a Bendayan, Moi͏̈se |e Verfasser |4 aut | |
| 830 | 0 | |a Progress in histochemistry and cytochemistry |v 36,1 |w (DE-604)BV000005277 |9 36,1 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017099916&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017099916 | ||
Datensatz im Suchindex
| _version_ | 1804138592868499456 |
|---|---|
| adam_text | Contents
1 Introduction 11
2 Pre-detection steps 12
2.1 Fixation 12
2.1.1 Chemical fixatives 13
2.1.2 Microwave-assisted fixation 14
2.2 Embedding 14
2.2.1 Embedding for light microscopy 15
2.2.2 Embedding for electron microscopy 16
2.2.2.1 Epoxy resins 16
2.2.2.2 Acrylic resins 17
2.3 Antigen retrieval for light and electron microscopy 19
2.3.1 Proteolytic pretreatment 20
2.3.2 Chemical reagents for antigen unmasking 21
2.3.3 Other pretreatments 21
2.3.4 Heat-mediated antigen retrieval 22
2.3.4.1 Proposed mechanisms 23
2.3.4.2 Antigen retrieval solutions 23
2.3.4.3 Heat-mediated antigen retrieval adapted for enhanced immunogold
labelings 24
3 Antigen-antibody reaction 26
3.1 Means of increasing the affinity of antibodies and antigen-antibody
complex formation 27
3.1.1 Genetically engineered antibodies 27
3.1.2 Novel monoclonal antibodies 27
3.1.3 Microwave and ultrasound-stimulated antigen-antibody complex for¬
mation 28
4 Amplified detection procedures 28
4.1 Direct versus indirect immunocytochemical techniques 28
4.2 Indirect detection systems 29
4.2.1 Immunofluorescence 29
4.2.2 Immunoenzymatic methods 31
4.2.2.1 Peroxidase-labeled antibodies and complexes 31
4.2.2.2 Multiple layer antibody methods 33
4.2.2.3 Enzyme-linked polymer amplification 35
4.2.3 Avidin-biotin systems 37
4.2.3.1 Avidin-biotin-enzyme cytochemistry 38
4.2.3.2 Enlarged complexes 39
4.2.3.3 Improved ABC complexes and anti-end-product antibody 39
4.2.3.4 Other avidin-biotin amplification approaches 41
6 • Contents
4.2.4 Colloidal gold immunocytochemistry 41
4.2.4.1 Protein-gold complexes 42
4.2.4.2 Quantitative evaluations 43
4.2.4.3 Colloidal gold amplification techniques 43
4.3 Catalytic deposition of derivatized tyramine by peroxidase 50
4.3.1 Mechanism of catalyzed reporter deposition 51
4.3.2 Marker-tagged HRP substrates 52
4.4 Signal intensification 55
5 Resolution 59
6 Concluding remarks 61
References 65
|
| any_adam_object | 1 |
| author | Mayer, Gaétan Bendayan, Moi͏̈se |
| author_facet | Mayer, Gaétan Bendayan, Moi͏̈se |
| author_role | aut aut |
| author_sort | Mayer, Gaétan |
| author_variant | g m gm m b mb |
| building | Verbundindex |
| bvnumber | BV035294941 |
| ctrlnum | (OCoLC)634969850 (DE-599)GBV323920012 |
| edition | [Bindeeinh.] |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01232nam a2200301 cb4500</leader><controlfield tag="001">BV035294941</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">090206s2001 ad|| |||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)634969850</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBV323920012</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-578</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Mayer, Gaétan</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Amplification methods for the immunolocalization of rare molecules in cells and tissues</subfield><subfield code="b">with 9 tables</subfield><subfield code="c">Gaétan Mayer ; Moı̈se Bendayan</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">[Bindeeinh.]</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Jena</subfield><subfield code="b">Urban & Fischer</subfield><subfield code="c">2001</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">85 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Progress in histochemistry and cytochemistry</subfield><subfield code="v">36,1</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bendayan, Moi͏̈se</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Progress in histochemistry and cytochemistry</subfield><subfield code="v">36,1</subfield><subfield code="w">(DE-604)BV000005277</subfield><subfield code="9">36,1</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017099916&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-017099916</subfield></datafield></record></collection> |
| id | DE-604.BV035294941 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:30:39Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017099916 |
| oclc_num | 634969850 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-578 |
| owner_facet | DE-91 DE-BY-TUM DE-578 |
| physical | 85 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Urban & Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Mayer, Gaétan Verfasser aut Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables Gaétan Mayer ; Moı̈se Bendayan [Bindeeinh.] Jena Urban & Fischer 2001 85 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 36,1 Bendayan, Moi͏̈se Verfasser aut Progress in histochemistry and cytochemistry 36,1 (DE-604)BV000005277 36,1 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017099916&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mayer, Gaétan Bendayan, Moi͏̈se Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables Progress in histochemistry and cytochemistry |
| title | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables |
| title_auth | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables |
| title_exact_search | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables |
| title_full | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables Gaétan Mayer ; Moı̈se Bendayan |
| title_fullStr | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables Gaétan Mayer ; Moı̈se Bendayan |
| title_full_unstemmed | Amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables Gaétan Mayer ; Moı̈se Bendayan |
| title_short | Amplification methods for the immunolocalization of rare molecules in cells and tissues |
| title_sort | amplification methods for the immunolocalization of rare molecules in cells and tissues with 9 tables |
| title_sub | with 9 tables |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017099916&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
| work_keys_str_mv | AT mayergaetan amplificationmethodsfortheimmunolocalizationofraremoleculesincellsandtissueswith9tables AT bendayanmoise amplificationmethodsfortheimmunolocalizationofraremoleculesincellsandtissueswith9tables |