DNA-protein interactions: principles and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
| Ausgabe: | 3. ed. |
| Schriftenreihe: | Methods in molecular biology
543 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 680 S. Ill., graph. Darst. |
| ISBN: | 9781603270144 9781603270151 |
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| 245 | 1 | 0 | |a DNA-protein interactions |b principles and protocols |c ed. by Tom Moss ... |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2009 | |
| 300 | |a XVII, 680 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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Datensatz im Suchindex
| _version_ | 1804138590736744448 |
|---|---|
| adam_text | Contents
Preface
.............................................................
v
Contributors
............................................................. xiii
1
Filter-Binding Assays
.............................................. 1
Peter G. Stockley
2
Electrophoretic Mobility Shift Assays for the Analysis
of DNA-Protein Interactions
........................................ 15
Manon
Gaudreault, Marie-Eve Ginjjras,
Maryse Lessard,
Steeve
Ledere,
and
Sylvain
L·
Guérin
3
DNase I Footprinting
.............................................. 37
Benoît Leblanc
and Tom Moss
4
Exonuclease III Footprinting on Immobilized
DNA
Templates
.............. 49
Patrizia
Spitalny and Michael Thomtn
5
Hydroxyl Radical Footprinting of Protein-DNA Complexes
................. 57
Indujagannathan and Jeffrey]. Hayes
6
The Use of Diethyl
Pyrocarbonate
and Potassium Permanganate
as Probes for Strand Separation and Structural Distortions in
DNA........... 73
Brenda P.
Kahl
and Marvin R.
Paule
7
Uranyl
Photofootprinting
........................................... 87
Peter
E.
Nielsen
8
Identification of Protein/DNA Contacts with Dimethyl
Sulfate: Methylation
Protection and Methylation Interference
............... 97
Peter E. Shaw and A. Francis Stewart
9
Ethylation Interference Footprinting of DNA-Protein Complexes
............ 105
Iain W. Manfield and Peter G. Stockley
10
Site-Directed Cleavage of
DNA
by Protein-Fe(II) EDTA Conjugates
Within Model Chromatin Complexes
.................................. 121
David R. Chafin and Jeffrey J. Hayes
11
Identification of Nucleic Acid High-Affinity Binding Sequences
of Proteins by SELEX
.............................................. 139
Philippe Bouvet
12
Identification of Sequence-Specific DNA-Binding Proteins
by Southwestern Blotting
........................................... 151
Simon
Labbé, Jean-François
Harrisson,
and Carl
Séguin
13 Footprinting DNA—Protein
Interactions in Native Polyacrylamide
Gels
by
Chemical
Nucleolytic Activity of l^O-Phenanthroline-Copper
......... 163
Athanasios G. Papavassiliou
14 Determination
of a Transcription Factor-Binding Site
by Nudease Protection Footprinting onto Southwestern Blots
............... 201
Athanasios G. Papavassiliou
IX
χ
Contents
15
Use of a Reporter Gene Assay in Yeast for Genetic Analysis
of DNA-Protein Interactions
........................................ 219
David R.
Setzer,
Deborah B. Schulman, Cathy V.
Günther,
and Michael J. Bumbulis
16
Chromatin Immunoprecipitation in Mammalian Cells
..................... 243
Amy Svotelis, Nicolas
Gévry,
and Luc Gaudreau
17
Sequential Chromatin Immunoprecipitation Protocol: ChlP-reChIP
.......... 253
Mayra Furlan-Magaril,
Héctor Rincón-Arano,
and
Félix
Recillas-Targa
18
Profiling Genome-Wide Histone Modifications and Variants
by ChlP-chip on Tiling Microarrays in <S. cerevisiae
........................ 267
Alain
R. Bataille
and
François
Robert
19
Nucleosome Mapping
............................................. 281
Nicolas
Gévry,
Amy Svotelis, Marc Larochelle, and Luc Gaudreau
20
In Cellulo
DNA
Analysis (LMPCR Footprinting)
......................... 293
Régen Drouin,
Nathalie Bastien,
Jean-François Millau,
François
Vigneault, and
Isabelle Paradis
21
Atomic Force Microscopy Imaging and Probing of
DNA,
Proteins, and Protein-DNA Complexes: Silatrane Surface Chemistry
.......... 337
Yuri L. Lyubchenko,
Luda
S. Shlyakhtenko,
and Alexander A, Gall
22
Two-Dimensional Crystallisation of Soluble Protein Complexes
.............. 353
Patrick
Schultz,
Corinne
Crucifix, and Luc Lebeau
23
Assays for Transcription Factor Activity
................................. 369
Douglas Browning, Nigel Savery, Annie
Kolb,
and Stephen Busby
24
Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
....... 389
Rainer Meffert,
Klaus Dose,
Gabriele
Rathgeber,
and
Hans-Jochen Schäfer
25
Static and Kinetic Site-Specific Protein-DNA Photocrosslinking:
Analysis of Bacterial Transcription Initiation Complexes
.................... 403
Nikolai Naryshkin, Sergei Druzhinin, Andrei Revyakin,
Tounggyu Kim, Vladimir Mekler, and
Richard H.
Ebright
26
Use of Site-Specific Protein—
DNA
Photocrosslinking
of Purified Complexes to Analyze the Topology of the
RNA
Polymerase II Transcription Initiation Complex
.......................... 439
Diane Forget,
Céline Domecq,
and
Benoit Coulombe
27
Site-Directed
DNA
Crosslinking of Large Multisubunit
Protein-DNA Complexes
........................................... 453
Jim
Persinger
and
Blatne
Bartholomew
28
Functional Studies of DNA-Protein Interactions
Using FRET Techniques
........................................... 475
Simon Blouin, Timothy D. Craggs, Daniel A.
Lafontaine,
and
J.
Carlos
Penedo
29
Single-Molecule FRET Analysis of Protein-DNA Complexes
................ 503
Mike Heilemann,
Ling Chin Hwang,
Konstantinos
Lymperopoulos, ttndAchillefsN. Kapanidis
Contents xi
30
Analysis of
DNA
Supercoiling Induced by DNA-Protein Interactions
.......... 523
David J.
Clark und
Benoît Leblanc
3
1
The Cruciform
DNA
Mobility Shift Assay: A Tool to Study
Proteins that Recognize Bent
DNA................................... 537
Victor T. Stefanovsky and Tom Moss
32
Plasmid Vectors for the Analysis of Protein-Induced
DNA
Bending
........... 547
Christian Zwieb and Sankar Adhya
33
Analysis of Distant Communication on Defined Chromatin
Templates In Vitro
................................................ 563
Yury S. Polikanov and Vastly M. Studitsky
34
A Competition Assay for
DNA
Binding Using the Fluorescent Probe
ANS...... 577
Ian A. Taylor and G. Geoff Kneale
35
Fluorescence Spectroscopy and Anisotropy in the Analysis
of DNA-Protein Interactions
........................................ 589
Rosy Favicchio, Anatoly
I. Dragan,
G.
Geoff Kneale, and
Christopher M. Read
36
Circular Dichroism for the Analysis of Protein-DNA Interactions
............. 613
P.D.
Cary
and G. Geoff Kneale
37
Defining the Thermodynamics of Protein/DNA Complexes
and Their Components Using Micro-calorimetry
......................... 625
Colyn Crane-Robinson, Anatoly
I. Dragan,
and Christopher M. Read
38
Surface Plasmon Resonance Assays of DNA-Protein Interactions
............. 653
Peter G. Stockley and
Björn Persson
Index
.............................................................. 671
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| spelling | DNA-protein interactions principles and protocols ed. by Tom Moss ... 3. ed. Totowa, NJ Humana Press 2009 XVII, 680 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 543 ADN ram DNA Footprinting methods Laboratory Manuals DNA-Binding Proteins chemistry Laboratory Manuals DNA-Binding Proteins genetics Laboratory Manuals DNA-protein interactions Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s DNS (DE-588)4070512-2 s Wechselwirkung (DE-588)4064937-4 s DE-604 Moss, Tom Sonstige oth Methods in molecular biology 543 (DE-604)BV035362695 543 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017098341&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA-protein interactions principles and protocols Methods in molecular biology ADN ram DNA Footprinting methods Laboratory Manuals DNA-Binding Proteins chemistry Laboratory Manuals DNA-Binding Proteins genetics Laboratory Manuals DNA-protein interactions Wechselwirkung (DE-588)4064937-4 gnd Proteine (DE-588)4076388-2 gnd DNS (DE-588)4070512-2 gnd |
| subject_GND | (DE-588)4064937-4 (DE-588)4076388-2 (DE-588)4070512-2 (DE-588)4143413-4 |
| title | DNA-protein interactions principles and protocols |
| title_auth | DNA-protein interactions principles and protocols |
| title_exact_search | DNA-protein interactions principles and protocols |
| title_full | DNA-protein interactions principles and protocols ed. by Tom Moss ... |
| title_fullStr | DNA-protein interactions principles and protocols ed. by Tom Moss ... |
| title_full_unstemmed | DNA-protein interactions principles and protocols ed. by Tom Moss ... |
| title_short | DNA-protein interactions |
| title_sort | dna protein interactions principles and protocols |
| title_sub | principles and protocols |
| topic | ADN ram DNA Footprinting methods Laboratory Manuals DNA-Binding Proteins chemistry Laboratory Manuals DNA-Binding Proteins genetics Laboratory Manuals DNA-protein interactions Wechselwirkung (DE-588)4064937-4 gnd Proteine (DE-588)4076388-2 gnd DNS (DE-588)4070512-2 gnd |
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