Genetic modification of hematopoietic stem cells: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2009
|
| Schriftenreihe: | Methods in molecular biology
506 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XXI, 494 S. Ill., graph. Darst. 27 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Genetic modification of hematopoietic stem cells |b methods and protocols |c ed. by Christopher Baum |
| 264 | 1 | |a New York, NY |b Humana Press |c 2009 | |
| 300 | |a XXI, 494 S. |b Ill., graph. Darst. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Methods in molecular biology |v 506 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Hematopoietic stem cells | |
| 650 | 4 | |a Hematopoietic Stem Cells | |
| 650 | 7 | |a Cellules souches hématopoïétiques - Génétique |2 ram | |
| 650 | 4 | |a Gene Therapy |x methods | |
| 650 | 4 | |a Hematopoietic Stem Cells | |
| 650 | 4 | |a Hematopoietic stem cells | |
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Datensatz im Suchindex
| _version_ | 1804138573035732992 |
|---|---|
| adam_text | Contents
Preface v
Contributors. xvii
1. Immunomagnetic Enrichment of Human and Mouse Hematopoietic
Stem Cells for Gene Therapy Applications 1
Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren
2. Isolation of Human and Mouse Hematopoietic Stem Cells 13
Tuk Tin Ng, Miranda R.M. Baert, Edwin F.E. de Haas,
Karin Pike-Overzet, and Frank J.T. Staal
3. Murine Hematopoietic Stem Cell Transduction
Using Retroviral Vectors 23
Ute Modlich, Axel Schambach, Zhixiong Li, and Bernhard Schiedlmeier
4. Genetic Modification of Human Hematopoietic Cells:
Preclinical Optimization of Oncoretroviral-mediated
Gene Transfer for Clinical Trials 33
Tulin Budak-Alpdogan and Isabelle Riviere
5. Short-Term Culture of Human CD34+ Cells
for Lentiviral Gene Transfer 59
Francesca Santoni de Sio and Luigi Naldini
6. T Cell Culture for Gammaretroviral Transfer 71
Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer
7. Retroviral Transduction of Murine Primary T Lymphocytes 83
James Lee, Michel Sadelain, and Renter Brentjens
8. Lentiviral Vector Gene Transfer into Human T Cells 97
Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset
9. DNA Transposons for Modification of Human Primary
T Lymphocytes 115
Xin Huang, Andrew Wilber, R. Scott Mclvor, and Xianzheng Zhou
10. Retroviral Gene Transfer into Primary Human Natural Killer Cells 127
Evren Alici, Tolga Sutlu, and M. Sirac Dilber
11. Lentiviral Vector-Mediated Genetic Programming of Mouse
and Human Dendritic Cells 139
Renata Stripecke
12. In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells 159
Dao Pan
13. In Vivo and Ex Vivo Gene Transfer in Thymocytes
and Thymocyte Precursors 171
Oumeya Adjali, Amelie Montel-Hagen, Louise Swainson,
Sophie Marty, Rita Vicente, Cedric Mongellaz, Chantal Jacquet,
Valerie Zimmermann, and Naomi Taylor
xiii
xiv Contents
14. Design and Production of Retro- and Lentiviral Vectors
for Gene Expression in Hematopoietic Cells 191
Axel Schambach, William P. Swaney, and Johannes CM. van der Loo
15. Knock-Down of Gene Expression in Hematopoietic Cells 207
Michaela Scherr, Letizia Venturing and Matthias Eder
16. The Use of Retroviral Vectors for tet-Regulated Gene
Expression in Cell Populations 221
Rainer Low
17. Detection of Replication Competent Retrovirus and Lentivirus 243
Lakshmi Sastry and Kenneth Cornetta
18. Release Testing of Retroviral Vectors and Gene-Modified Cells 265
Diana Nordling, Anne Kaiser, and Lilith Reeves
19. Copy Number Determination of Genetically-Modified
Hematopoietic Stem Cells 281
Todd Schuesler, Lilith Reeves, Christofvon Kalle,
and Elke Grassman
20. Tissue Procurement for Molecular Studies
Using Laser-Assisted Microdissection 299
Ulrich Lehtnann and Hans Kreipe
21. Leukemia Diagnosis in Murine Bone Marrow Transplantation Models 311
Zhixionjj Li, Ute Modlich, and Anjali Mishra
22. Humanized Mouse Models to Study the Human Haematopoietic
Stem Cell Compartment 331
Dominique Bonnet
23. Canine Models of Gene-Modified Hematopoiesis 341
Brian C. Beard and Hans-Peter Kiem
24. Detection of Retroviral Integration Sites by Linear
Amplification-Mediated PCRand Tracking of Individual
Integration Clones in Different Samples 363
Manfred Schmidt, Kerstin Schwarzwaelder,
Cynthia C. Bartholomew, Hanno Glimm, and Christofvon Kalle
25. Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones 373
Olga S. Kustikova, Ute Modlich, and Boris Fehse
26. Tracking Gene-Modified T Cells In Vivo 391
Alessandra Recchia and Fulvio Mavilio
27. DNA Microarray Studies of Hematopoietic Subpopulations 403
Karin Pike-Overzet, Dick de Ridder, Tom Schonewille,
and Frank J.T. Staal
28. Quantification of Genomic Mutations in Murine
Hematopoietic Cells 423
Hartmut Geiger, David Schleimer,
KalpanaJ. Nattamai, and Jan Vijg
29. Proteomics Studies After Hematopoietic Stem Cell Transplantation 437
Eva M. Weissinger, Petra Ziirbig, and Arnold Ganser
Contents xv
30. Spectral Karyotyping and Fluorescence
In Situ Hybridization of Murine Cells 453
Cornelia Rudolph and Brigitte Schlegelberger
31. Database Setup for Preclinical Studies
of Gene-Modified Hematopoiesis 467
Brenden Balcik, Elke Grassman, and Lilitb Reeves
32. The US and EU Regulatory Perspectives on the Clinical
Use of Hematopoietic Stem/Progenitor Cells Genetically
Modified Ex Vivo by Retroviral Vectors 477
Carolyn A. Wilson and Klaus Cichutek
Index 489
|
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| spelling | Genetic modification of hematopoietic stem cells methods and protocols ed. by Christopher Baum New York, NY Humana Press 2009 XXI, 494 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 506 Includes bibliographical references and index Hematopoietic stem cells Hematopoietic Stem Cells Cellules souches hématopoïétiques - Génétique ram Gene Therapy methods Blutstammzelle (DE-588)4146093-5 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf Blutstammzelle (DE-588)4146093-5 s Genanalyse (DE-588)4200230-8 s b DE-604 Baum, Christopher 1962- Sonstige (DE-588)133797821 oth Methods in molecular biology 506 (DE-604)BV035362695 506 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017086034&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Genetic modification of hematopoietic stem cells methods and protocols Methods in molecular biology Hematopoietic stem cells Hematopoietic Stem Cells Cellules souches hématopoïétiques - Génétique ram Gene Therapy methods Blutstammzelle (DE-588)4146093-5 gnd Genanalyse (DE-588)4200230-8 gnd |
| subject_GND | (DE-588)4146093-5 (DE-588)4200230-8 |
| title | Genetic modification of hematopoietic stem cells methods and protocols |
| title_auth | Genetic modification of hematopoietic stem cells methods and protocols |
| title_exact_search | Genetic modification of hematopoietic stem cells methods and protocols |
| title_full | Genetic modification of hematopoietic stem cells methods and protocols ed. by Christopher Baum |
| title_fullStr | Genetic modification of hematopoietic stem cells methods and protocols ed. by Christopher Baum |
| title_full_unstemmed | Genetic modification of hematopoietic stem cells methods and protocols ed. by Christopher Baum |
| title_short | Genetic modification of hematopoietic stem cells |
| title_sort | genetic modification of hematopoietic stem cells methods and protocols |
| title_sub | methods and protocols |
| topic | Hematopoietic stem cells Hematopoietic Stem Cells Cellules souches hématopoïétiques - Génétique ram Gene Therapy methods Blutstammzelle (DE-588)4146093-5 gnd Genanalyse (DE-588)4200230-8 gnd |
| topic_facet | Hematopoietic stem cells Hematopoietic Stem Cells Cellules souches hématopoïétiques - Génétique Gene Therapy methods Blutstammzelle Genanalyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017086034&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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