Gene knockout protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2009
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
530 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 516 S. Ill., graph. Darst. |
| ISBN: | 9781627038348 9781934115268 9781597454711 |
Internformat
MARC
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| 245 | 1 | 0 | |a Gene knockout protocols |c ed. by Ralf Kühn ... |
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| 300 | |a XVI, 516 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 2 | |a Souris transgéniques | |
| 650 | 4 | |a Gene Knockout Techniques |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Gene targeting | |
| 650 | 4 | |a Transgenic mice | |
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Datensatz im Suchindex
| _version_ | 1804138571852939264 |
|---|---|
| adam_text | Contents
Preface
............................................................
v
Contributors
........................................................ ix
Color Plates
........................................................ xv
1.
Overview on Mouse Mutagenesis
...................................... 1
Ralf Kühn
and Wolfgang
Wurst
Part I: Gene Modification in
ES
Cells
2.
Construction of Gene-Targeting Vectors by Recombineering
................ 15
Sonß-Choon
Lee, Wei
Wanß,
ani Pentao
Liu
3.
Gene-Trap Vectors and Mutagenesis
................................... 29
Silke De-Zolt,
Joachim
Altschmied,
Patricia Ruiz,
Harald von Melchner,
and
Frank
Schnütgen
4.
Chromosome Engineering in
ES
Cells
.................................. 49
Louise van
der Weyden,
Charles Shaw-Smith, and Allan Bradley
5.
Gene Modification in Embryonic Stem Cells by Single-Stranded
DNA
Oligonucleotides
................................................... 79
Mariéke
Aarts, Marleen Dekker, Rob Dekker,
Sandra
de Vries,
Anja
van der Wal,
Eva Wielders, and Hein
te Riele
6. Generation
of shRNA Transgenic Mice
................................. 101
Christiane
Hitz, Patricia Steuber-Buchberger, Sabit
Delie,
Wolfyang Wurst,
and
Ralf Kühn
7. Mutagenesis
of Mouse Embryonic Stem Cells with Ethylmethanesulfonate
...... 131
Robert Munroe and John Schimenti
Part II: Stem Cell Manipulation
8.
Gene Targeting in Mouse Embryonic Stem Cells
.......................... 141
Lino Tessarollo, Mary Ellen Palko, Keiko
Akągi,
and
Vincenzo
Coppola
9.
Manipulating Mouse Embryonic Stem Cells
.............................. 165
Eileen Southon and Lino Tessarollo
10. ES
Cell Line Establishment
........................................... 187
Heidrun Kern
and
Branko Zevnik
11.
Generation of Double-Knockout Embryonic Stem Cells
.................... 205
Eva Wielders,
Marleen Dekker,
and
Hein te
Riele
12. Differentiation
Analysis of Pluripotent Mouse Embryonic Stem
(ES)
Cells
In Vitro
......................................................... 219
Insa
S.
Schroeder, Cornelia
Wiese,
Thuy T. Truong, Alexandra Rolletschek,
and Anna M. Wobus
13.
Cloning of
ES
Cells and Mice by Nuclear Transfer
......................... 251
Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama
VII
viii Contents
Part III: Genetically Engineered Mice
14.
Isolation, Microinjection and Transfer of Mouse Blastocysts
................. 269
Susan W. Reid and Lino Tessarollo
15.
Aggregation Chimeras: Combining
ES
Cells, Diploid, and Tetraploid
Embryos
......................................................... 287
Mika
Tanaka, Anna-Katerina Hadjantonakis,
Kristina Vintersten,
and
Andras
Nagy
16.
VelociMouse: Fully
ES
Cell-Derived FO-Generation Mice Obtained from the
Injection of
ES
Cells into Eight-Cell-Stage Embryos
....................... 311
Thomas M. DeCMara, William T. Poueymirou,
Wojtek Auerbach,
David Frendewey, George D. Yancopoulos, and David M. Valenzuela
17.
Generation of Cre
Recombinase-
Expressing Transgenic Mice Using Bacterial
Artificial Chromosomes
............................................. 325
Jan Rodriguez Parkitna, David Engblom, and
Günther Schütz
18.
Inducible Cre Mice
................................................. 343
Susanne Feil,
Nadejdą
Valtcheva, and Robert
Feil
19.
Creation and Use of a Cre
Recombinase
Transgenic Database
................ 365
Andras
Nagy,
Lynn Mar, and Graham Watts
20.
Transposon Mutagenesis in Mice
...................................... 379
David A. Largaespada
21.
Lentiviral
Transgenesis.............................................. 391
Alexander
Pfeifer
and Andreas
Hofmann
22.
Sperm Cryopreservation and In Vitro Fertilization
......................... 407
Susan
Marschall,
Auke Boersma, and Martin
Hrabe
de
Angelis
Part IV: Phenotype Analysis
23.
Influence of Genetic Background on Genetically Engineered
Mouse Phenotypes
................................................. 423
Thomas Doetschman
24.
Pathologic Phenotyping of Mutant Mice
................................ 435
Roderick T. Branson
25.
Systemic First-Line Phenotyping
...................................... 463
Valerie Gailus-Durner, Helmut
Fuchs,
Thure
Adler,
Antonio Aguilar
Pimentel, Lore Becker,
Ines
Bolle,
Julia Calzada-Wack, Claudia Dalke,
Nicole Ehrhardt, Barbara Ferwagner,
Wolfyang
Hans,
Sabine
M.
Halter,
Gabriele Hölzlwimmer, Marion Horsch, Anahita Javaheri, Magdalena
Kallnik, Eva Kling, Christoph Lengger, Corinna Mörth, Ilona Mossbrugger,
Beatrix
Naton,
Cornelia Prehn, Oliver
Puk,
Birgit Rathkolb, Jan Rozman,
Anja Schrewe, Frank Thiele,
Jerzy
Adamski, Bernhard Aigner, Heidrun
Behrendt, Dirk H. Busch, Jack
Favor,
Jochen Graw, Gerhard Heldmaier,
Boris Ivandic, Hugo
Katus,
Martin Klingenspor,
Thomas Klopstock, Elisabeth Kremmer, Markus Ollert,
Leticia
Quintanilla-Martinez, Holger Schulz, Eckhard Wolf,
Wolfgang Wurst, and Martin
Hrabe
de Angelis
Index................................................................ 511
|
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| ctrlnum | (OCoLC)276226992 (DE-599)BVBBV035280027 |
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| discipline | Biologie Chemie-Ingenieurwesen Biotechnologie |
| edition | 2. ed. |
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| id | DE-604.BV035280027 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:30:19Z |
| institution | BVB |
| isbn | 9781627038348 9781934115268 9781597454711 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017085249 |
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| physical | XVI, 516 S. Ill., graph. Darst. |
| publishDate | 2009 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
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| spelling | Gene knockout protocols ed. by Ralf Kühn ... 2. ed. New York, NY Humana Press 2009 XVI, 516 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 530 molecular biology cabt genetics cabt molecular genetics cabt Ciblage gène Génétique ram Souris transgéniques Gene Knockout Techniques methods Laboratory Manuals Gene targeting Transgenic mice Mutante (DE-588)4256709-9 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf Gen-Targeting (DE-588)4294998-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Mutante (DE-588)4256709-9 s Gen (DE-588)4128987-0 s DE-604 Transgene Tiere (DE-588)4279021-9 s Gen-Targeting (DE-588)4294998-1 s Kühn, Ralf Sonstige oth Methods in molecular biology 530 (DE-604)BV035362695 530 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017085249&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gene knockout protocols Methods in molecular biology molecular biology cabt genetics cabt molecular genetics cabt Ciblage gène Génétique ram Souris transgéniques Gene Knockout Techniques methods Laboratory Manuals Gene targeting Transgenic mice Mutante (DE-588)4256709-9 gnd Transgene Tiere (DE-588)4279021-9 gnd Maus (DE-588)4169148-9 gnd Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd |
| subject_GND | (DE-588)4256709-9 (DE-588)4279021-9 (DE-588)4169148-9 (DE-588)4128987-0 (DE-588)4294998-1 (DE-588)4143413-4 |
| title | Gene knockout protocols |
| title_auth | Gene knockout protocols |
| title_exact_search | Gene knockout protocols |
| title_full | Gene knockout protocols ed. by Ralf Kühn ... |
| title_fullStr | Gene knockout protocols ed. by Ralf Kühn ... |
| title_full_unstemmed | Gene knockout protocols ed. by Ralf Kühn ... |
| title_short | Gene knockout protocols |
| title_sort | gene knockout protocols |
| topic | molecular biology cabt genetics cabt molecular genetics cabt Ciblage gène Génétique ram Souris transgéniques Gene Knockout Techniques methods Laboratory Manuals Gene targeting Transgenic mice Mutante (DE-588)4256709-9 gnd Transgene Tiere (DE-588)4279021-9 gnd Maus (DE-588)4169148-9 gnd Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd |
| topic_facet | molecular biology genetics molecular genetics Ciblage gène Génétique Souris transgéniques Gene Knockout Techniques methods Laboratory Manuals Gene targeting Transgenic mice Mutante Transgene Tiere Maus Gen Gen-Targeting Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017085249&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT kuhnralf geneknockoutprotocols |