Fluorescence spectroscopy:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, AP
2008
|
| Schriftenreihe: | Methods in enzymology
450 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLII, 358 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123745866 |
Internformat
MARC
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|---|---|---|---|
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| 005 | 20100906 | ||
| 007 | t | ||
| 008 | 090120s2008 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123745866 |9 978-0-12-374586-6 | ||
| 035 | |a (OCoLC)300399208 | ||
| 035 | |a (DE-599)BVBBV035258708 | ||
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| 245 | 1 | 0 | |a Fluorescence spectroscopy |c ed. by Ludwig Brand ... |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, AP |c 2008 | |
| 300 | |a XLII, 358 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 450 | |
| 650 | 4 | |a Fluorescence spectroscopy | |
| 650 | 4 | |a Spectrometry, Fluorescence |x methods | |
| 650 | 0 | 7 | |a Fluoreszenzspektroskopie |0 (DE-588)4017701-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
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| 830 | 0 | |a Methods in enzymology |v 450 |w (DE-604)BV000000938 |9 450 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-017064260 | ||
Datensatz im Suchindex
| _version_ | 1804138540745883648 |
|---|---|
| adam_text | Contents
Contributors
χ/
Preface xv
Volumes in Series
xvii
1.
A Method in Enzymology for Measuring Hydrolytic
Activities in Live Cell Environments
1
Beverly Z. Packard and Akira Komoriya
1.
Protease Substrate Design
2
2.
Protease Specificity
5
3.
Cell-Permeable Fluorogenic Probes
7
4.
Conclusions
16
References
17
2.
Heterogeneity of Fluorescence Determined by the Method of
Area-Normalized Time-Resolved Emission Spectroscopy
21
N.
Periasamy
1.
Introduction
22
2.
TRES
and
TRANES
Methods
24
3.
TRES
and
TRANES
Spectra of Simple Cases
25
4.
Physical Significance of
TRANES
and Isoemissive Point
29
5.
TRES
and
TRANES
of Fluorophores in Microheterogeneous Media
30
6.
Fluorescence in Microheterogeneous and Biological
Media: Special Cases
32
Acknowledgment
33
References
33
3.
Multiparametric Probing of
Microenvironment
with
Solvatochromic Fluorescent Dyes
37
Andrey S.
Ktymchenko and Alexander P. Demchenko
1.
Universal and Specific Noncovalent Interactions
39
2.
The Methodology of Multiparametric Approach with Application of
3-Hydroxyflavone Dyes
40
3.
Correlations of
Spectroscopie
Data with Solvatochromic Variables
43
v¡
Contents
4.
Algorithm for Multiparametric Probing Based on Parameters
of Absorption and Dual Emission
47
5.
Application of Multiparametric Probing
49
6.
Limitations of the Multiparametric Approach
51
7.
Concluding Remarks
55
References
56
4.
Site-Selective Red-Edge Effects
59
Alexander P. Demchenko
1.
Introduction
60
2.
Molecular Disorder and the Origin of Red-Edge Effects
61
3.
The Principle of Photoselection
62
4.
Ground-State Heterogeneity
65
5.
The Magnitude of Red-Edge Excitation Fluorescence
Shift and Its Connection with Dielectric Relaxations
67
6.
Red-Edge Effect with High Resolution in Time
71
7.
Red-Edge Effects in Excited-State Reactions
72
8.
Fluorescent Probes for Optimal Observation of Red-Edge Effects
74
9.
Peculiarities of the Red-Edge Effects of
Indole
and Tryptophan
75
10.
Conclusions
76
References
77
5.
Fluorescence Approaches to Quantifying
Biomolecular Interactions
79
Catherine A.
Royer
and Suzanne F. Scarlata
1.
Introduction
80
2.
Fluorescence
Observables
80
3.
Designing a Fluorescence Experiment
85
4.
Conclusions
103
References
103
6. Förster
Resonance Energy Transfer Measurements
of
Transmembrane
Helix Dimerization Energetics
107
Mikhail Merzlyakov and
Kalina
Hristova
1.
Introduction
108
2.
Challenges in Quantitative Measurements of Interactions
Between TM Helices in Bilayers
108
3.
Bilayer Platforms for Measuring TM Helix Interactions Using FRET
110
4.
FRET Due to Random Colocalization of Donors and
Acceptors (Proximity FRET)
114
Contents
vii
5.
FRET Efficiencies and Energetics of
TM
Helix
Dimerization 115
6.
Biological Insights from FRET Measurements
124
7.
Conclusion
125
References
125
7.
Application of Single-Molecule Spectroscopy in
Studying Enzyme Kinetics and Mechanism
129
Jue Shi, Joseph Dertouzos,
Ari Gafni,
and Duncan Steel
1.
Introduction
130
2.
Experimental Considerations
134
3.
Sample Preparation
136
4.
Instrumentation
138
5.
Data Analysis of Single-Molecule Trajectories
141
6.
Additional Considerations
151
7.
Summary
154
References
155
8.
Ultrafast Fluorescence Spectroscopy via
Upconversion: Applications to Biophysics
159
Jianhua Xu and Jay R.
Knutson
1.
Introduction
160
2.
Basic Concepts
162
3.
Upconversion Spectrophotofluorometer and
Experimental Considerations
168
4.
Ultrafast Photophysics of Single Tryptophan, Peptides,
Proteins, and Nucleic Acids
171
5.
Summary and Future Directions
179
References
179
9.
Use of Fluorescence Resonance Energy Transfer
(FRET) in Studying Protein-induced
DNA
Bending
185
Anatoly
I. Dragan
and Peter
L. Prívalov
1.
Introduction
186
2.
Preparation of Labeled
DNA
Duplexes
187
3.
Fluorescence Resonance Energy Transfer
189
4.
FRET in Studying Large Protein-Induced
DNA
Bends
191
5.
Dependence of the Protein-induced
DNA
Bend on the
Forces Involved in Binding
194
6.
FRET in Studying Small Protein-Induced
DNA
Bends
194
7.
Conclusions
198
v¡¡¡
Contents
Acknowledgment
198
References
199
10.
Fluorescent Pteridine Probes for Nucleic Acid Analysis
201
Mary E. Hawkins
1.
introduction
202
2.
Pteridine Analog Characteristics
205
3.
Procedures for Oligonucleotide Synthesis with Pteridine Analogs
208
4.
Characterization of Pteridine-Containing Sequences
210
5.
Applications
211
6.
Summary
227
Acknowledgments
228
References
228
11.
Single-Molecule Fluorescence Methods for the Analysis of
RNA
Folding and Ribonucleoprotein Assembly
233
Goran
Pljevaljčić
and David P. Millar
1.
Introduction
233
2.
Labeling Methods
236
3.
Single-Molecule Fluorescence Detection Methods
238
4.
Diffusion Single-Pair FRET for
RNA
Based Systems
238
5.
Total Internal Reflection Fluorescence (TIRF) for
RNA
Based Systems
240
6.
Application
1:
Folding of the Hairpin Ribozyme
243
7.
Application
2:
Assembly of the Rev-RRE Complex
247
Acknowledgments
249
References
249
12.
Using Fluorophore-Labeled Oligonucleotides to
Measure Affinities of Protein-DNA Interactions
253
Brian J. Anderson, Chris Larkin,
Kip Guja,
and Joel
F. Schildbach
1.
Introduction
254
2.
Definitions of Fluorescence Anisotropy and Intensity
254
3.
Advantages of Fluorescence Measurements
256
4.
Disadvantages of Fluorescence Measurements
257
5.
Designing the Oligonucleotide
258
6.
Designing the Experiment
266
7.
Competition Assays for Determining Specificity
269
8.
Conclusions
270
Acknowledgments
270
References
270
Contents
¡x
13.
Identifying Small Pulsatile Signals within Noisy Data:
A Fluorescence Application
273
Michael L. Johnson, Leon S. Farhy, Paula P.
Veldhuis,
and Joseph R. Lakowicz
1.
Introduction
274
2.
Methods
274
3.
Results
280
4.
Discussion
284
Acknowledgments
286
References
286
14.
Determination of Zinc Using Carbonic Anhydrase-Based
Fluorescence Biosensors
287
Rebecca Bozym, Tamiika K. Hurst, Nissa
Westerberg,
Andrea Stoddard,
Carol A. Fierke, Christopher J. Frederickson, and Richard B. Thompson
1.
Introduction
288
2.
Principles of CA-Based Zinc Sensing
288
3.
Transducing Zinc Binding as a Fluorescence Change
290
4.
Free Versus Bound Zinc Ion: Speciation
295
5.
Metal Ion Buffers
297
6.
Kinetics
301
7.
Applications: Ratiometric Determination of Free Zinc in Solution
302
8.
Preparation of Apocarbonic Anhydrase
303
9.
Intracellular Sensing with TAT Tag
304
10.
Intracellular Sensing with an Expressible CA Sensor
305
References
307
15.
Instrumentation for Fluorescence-Based Fiber Optic Biosensors
311
Richard B. Thompson,
Hui-Hui Zeng,
Daniel Ohnemus,
Bryan McCranor,
Michele
Cramer, and James Moffett
1.
Introduction and Rationale for Fluorescence-Based
Fiber Optic Sensors
312
2.
Basic Principles of Fiber Optics for Fluorescence Sensors
313
3.
Optics and Mechanics of Fluorescence-Based Fiber Optic Sensors
316
4.
Mounting and Alignment of the Instrument
319
5.
Standards for Ratiometric and Lifetime-Based Fiber Optic
Fluorescence Sensors
323
6.
Construction of Fiber Optic Probes
326
7.
Zn2+ Probe
329
8.
Cu2+ Probe
330
9.
Use of Fiber Optic Probes for Discrete Samples
332
Contents
10.
Operating Issues: Noise, Background, Thermal Drift, and
Mode Hopping
333
11.
Field and Shipboard Use
334
Acknowledgments
335
References
336
Author Index
339
Subject Index
353
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| id | DE-604.BV035258708 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:29:49Z |
| institution | BVB |
| isbn | 9780123745866 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017064260 |
| oclc_num | 300399208 |
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| physical | XLII, 358 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
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| publisher | Elsevier, AP |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Fluorescence spectroscopy ed. by Ludwig Brand ... Amsterdam [u.a.] Elsevier, AP 2008 XLII, 358 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 450 Fluorescence spectroscopy Spectrometry, Fluorescence methods Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Fluoreszenzspektroskopie (DE-588)4017701-4 s b DE-604 Brand, Ludwig Sonstige (DE-588)137343906 oth Methods in enzymology 450 (DE-604)BV000000938 450 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017064260&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fluorescence spectroscopy Methods in enzymology Fluorescence spectroscopy Spectrometry, Fluorescence methods Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| subject_GND | (DE-588)4017701-4 (DE-588)4143413-4 |
| title | Fluorescence spectroscopy |
| title_auth | Fluorescence spectroscopy |
| title_exact_search | Fluorescence spectroscopy |
| title_full | Fluorescence spectroscopy ed. by Ludwig Brand ... |
| title_fullStr | Fluorescence spectroscopy ed. by Ludwig Brand ... |
| title_full_unstemmed | Fluorescence spectroscopy ed. by Ludwig Brand ... |
| title_short | Fluorescence spectroscopy |
| title_sort | fluorescence spectroscopy |
| topic | Fluorescence spectroscopy Spectrometry, Fluorescence methods Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
| topic_facet | Fluorescence spectroscopy Spectrometry, Fluorescence methods Fluoreszenzspektroskopie Aufsatzsammlung |
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