Essential zebrafish methods: cell and developmental biology
The methods and techniques in this volume were chosen by the editors of 'Methods in Cell Biology', whose goal was to provide fail-safe methods, tips, and 'tricks of the trade' to experienced researchers and more junior members in the lab.
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
2009
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Reliable lab solutions
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | The methods and techniques in this volume were chosen by the editors of 'Methods in Cell Biology', whose goal was to provide fail-safe methods, tips, and 'tricks of the trade' to experienced researchers and more junior members in the lab. |
| Beschreibung: | XVIII, 546 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123745996 |
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| 650 | 2 | |a Danio zébré - méthodes | |
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Datensatz im Suchindex
| _version_ | 1804138525877075968 |
|---|---|
| adam_text | Titel: Essential zebrafish methods
Autor: Detrich, H. William
Jahr: 2009
CONTENTS
Contributors
Preface
1. Haploid Screens and Gamma-Ray Mutagenesis
Charline Walker
Update 2
I. Introduction 2
II. Production of Haploid Embryos 3
III. Development of Haploid Embryos 6
IV. Genetic Background 8
V. The Haploid Screen 10
VI. Limitations of Haploids 14
VII. Mosaicism in Ft Screens 16
VIII. Haploid Screens and Gamma-Ray Mutagenesis 17
IX. Nature of Gamma-Ray-Induced Mutations 21
X. Conclusion 24
References 25
2. Positional Cloning of Mutated Zebrafish Genes
William S. Talbot and Alexander F. Schier
30
30
32
36
39
41
42
42
44
Update
I. Introduction
II. Initiating a Positional Cloning Project
III. Defining the Critical Region
IV. Gene Discovery
V. Protocol 1
VI. Protocol 2
VII. Protocol 3 References
Contents
3. The Zebrafish Genome
John Postlethwait, Angel Amores, Allan Force, and Yi-Lin Yan
I. Introduction 47
II. Mapping the Zebrafish Genome 48
III. The Zebrafish Gene Map 50
IV. Two Genes in Zebrafish for One in Mammals 54
V. Gene Nomenclature in Zebrafish 56
VI. Conclusions 37
References 58
4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish
Adam Amsterdam and Nancy Hopkins
Update 61
I. Introduction °2
II. Mutagenesis ^
III. Cloning the Mutated Genes
IV. Future Directions
References
5. Genetic Screens for Maternal-Effect Mutations
Francisco Pelegri and Mary C. Mullins
Update g2
I. Introduction
II. Strategies for Maternal-Effect Screens
III. Selection of Lines for Genetic Screens
IV. Recovery and Maintenance of Maternal-Effect Mutations 98
101
V. Mapping Maternal-Effect Mutations lv
VI. Solutions, Materials, and Protocols
VII. Conclusions
References
Behavioral Screening Assays in Zebrafish
Michael B. Orger, Ethan Gahtan, Akira Muto, Patrick Page-McCaw,
Matthew C. Smear, and Herwig Baier
I. Introduction
II. General Considerations
III. Behavioral Assays
IV. Conclusions
References
83
84
95
109
110
113
115
116
126
126
Contents
7. TILLING the Zebrafish Genome: A Reverse Genetics Approach
Bruce W. Draper, Claire M. McCallum, Jennifer L. Stout, and Cecilia B. Moens
I. Introduction 132
II. Rationale for Reverse Genetics in Zebrafish 135
III. Rationale for Using the CEL1 Assay to Detect ENU-Induced Mutations 136
IV. Rationale for Generating a Cryopreserved Mutant Library 138
V. Method of N-Ethyl-N-Nitrosourea (ENU) Mutagenesis and
Rearing of F! Founder Fish 138
VI. Generating a Cryopreserved Mutant Library 139
VII. Isolating Genomic DNA 142
VIII. Choosing Fragments to Screen 144
IX. CEL1 Endonuclease Assay 144
X. Recovery of Mutations from Cryopreserved Sperm 149
XL Materials Cost Estimate 150
References 151
8. The Transgenesis and Gene and Enhancer Trap Methods in Zebrafish by
Using the Tol2 Transposable Element
Koichi Kawakami
I. Introduction 153
II. Transgenesis by Using the Tol2 Transposable Element in Zebrafish 155
III. Gene Trap and Enhancer Trap Approaches That Use the
Tol2 Transposon System in Zebrafish 161
IV Summary and Perspectives 169
References 171
9. Molecular Cytogenetic Methodologies and a Second-Generation BAC
Probe Panel Resource for Zebrafish Genomic Analyses
Charles Lee, Danielle Palmer, Jennifer L. Freeman, and Kim H. Brown
Update 175
I. Introduction 176
II. Methods 177
III. Second-Generation Zebrafish BAC Probe Panel 184
References 188
10. The Zon Laboratory Guide to Positional Cloning in Zebrafish
Yi Zhou, Nathan Bahary, Alan Davidson, David Ransom, Jennifer Shepard,
Howard Stern, Nikilaus Trede, Bruce Barut, and Leonard I. Zon
I. Introduction 192
II. Mapping Strains 192
Contents
III. Families and Genetic Markers 193
IV. Crosses for Line Maintenance and Mapping 193
V. Preparation of the DNA 195
VI. Mapping Genes 196
VII. Overgo Strategy for Rapid Chromosomal Walks and Positional Cloning 206
VIII. Protocol for Overgo Probing of High-Density Filters 207
General Flow of Information from the Radiation Hybrid Panel
Maps, the Sanger Institute Sequencing Project, and Fingerprinting the BACs 209
Synteny Between Human, Zebrafish, Fugu, and Tetraodon Genomes 213
Proving a Candidate Gene Is Responsible for the Mutant Phenotype 215
XII. Morpholinos 216
References 216
IX.
X.
XI.
11. Sleeping Beauty Transposon for Efficient Gene Delivery
Spencer Hermanson, Ann E. Davidson, Sridhar Sivasubbu,
Darius Bakiunas, and Stephen C. Ekker
Update
I. Introduction
II. Transgenesis Constructs
III. Microinjection of the Zebrafish Embryo
IV. Raising Injected Embryos
V. Identifying Transgenic Founders
VI. Visualizing Fluorescent Reporters
VII. Molecular Characterization of the Transposon Integration Site
References
219
220
220
222
224
227
227
228
232
12. Transgene Manipulation in Zebrafish by Using Recombinases
Yang Bai,Jie Dong, and Gary W. Stuart
I. Introduction
II. Recombinase-Mediated Transgene Exchange and Mobilization
III. Summary and Conclusion
References
233
238
248
249
13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-Scel
Clemens Grabher, Jean-Stephane Joly, and Joachim Wittbrodt
I. Introduction
II. Transgenesis by Meganucleases
References
255
260
273
Contents
14. Cloning Zebrafish by Nuclear Transfer
Haigen Huang, BenshengJu, Ki-Young Lee, and Shuo Lin
I. Introduction 277
II. Recipes for Cell Culture and Nuclear Transfer 278
III. Cell Culture 278
IV. Nuclear Transfer 279
V. Summary of Nuclear Transfer 282
VI. Potential Applications of Zebrafish Cloning 283
References 284
15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale
In Situ Hybridization Screening
Bernard Thisse, Vincent Heyer, Aline Lux, Violaine Alunni, Agnes Degravc,
Iban Seiliez, Johanne Kirchner, Jean-Paul ParkhiU, and Christine Thisse
I. Introduction and Goals 288
II. Preparation of Antisense Digoxigenin (DIG)-Labeled RNA Probes 288
III. Preparation of Embryos 290
IV. Reagents and Buffers for In Situ Hybridization 291
V. In Situ Hybridization Protocol 292
VI. Double In Situ Protocol 296
VII. Recording Results 297
VIII. Concluding Remarks 300
References 300
16. Genetic Backgrounds, Standard Lines, and Their Husbandry
Bill Trevarrow and Barrie Robison
Update 303
I. Introduction 306
II. Nomenclature and Definitions 307
III. Goals for Line Use 307
IV. Breeding Strategies 308
V Cryopreservation 316
VI. Monitoring and Response 317
VII. Distribution 318
VIII. Summary and Recommendations 318
References 319
Contents
17. Common Diseases of Laboratory Zebrafish
Jennifer L. Matthews
I. Introduction 321
II. Diagnostic Evaluation 321
III. Common Diseases of Laboratory Zebrafish 322
IV. Zoonosis 343
References 344
18. Zebrafish Sperm Cryopreservation
Stephane Berghmans, John P. Morris IV, John P. Kanki, and A. Thomas Look
Update 347
I. Introduction: Benefits of Zebrafish Sperm Cryopreservation 348
II. Critical Variables Affecting Sperm Cryopreservation 349
III. Zebrafish Sperm Cryopreservation with N,N-Dimethylacetamide 352
IV. Future Directions 360
References 361
Index 365
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| spelling | Essential zebrafish methods cell and developmental biology ed. by H. William Detrich ... 1. ed. Amsterdam [u.a.] Elsevier 2009 XVIII, 546 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Reliable lab solutions The methods and techniques in this volume were chosen by the editors of 'Methods in Cell Biology', whose goal was to provide fail-safe methods, tips, and 'tricks of the trade' to experienced researchers and more junior members in the lab. Danio zébré - génétique Danio zébré - méthodes Zebra danio / Genetics / Research Zebra danios as laboratory animals / Laboratory manuals Zebrabärbling (DE-588)4079468-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Zebrabärbling (DE-588)4079468-4 s Methode (DE-588)4038971-6 s DE-604 Detrich, H. William Sonstige (DE-588)1013514475 oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017053822&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Essential zebrafish methods cell and developmental biology Danio zébré - génétique Danio zébré - méthodes Zebra danio / Genetics / Research Zebra danios as laboratory animals / Laboratory manuals Zebrabärbling (DE-588)4079468-4 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4079468-4 (DE-588)4038971-6 |
| title | Essential zebrafish methods cell and developmental biology |
| title_auth | Essential zebrafish methods cell and developmental biology |
| title_exact_search | Essential zebrafish methods cell and developmental biology |
| title_full | Essential zebrafish methods cell and developmental biology ed. by H. William Detrich ... |
| title_fullStr | Essential zebrafish methods cell and developmental biology ed. by H. William Detrich ... |
| title_full_unstemmed | Essential zebrafish methods cell and developmental biology ed. by H. William Detrich ... |
| title_short | Essential zebrafish methods |
| title_sort | essential zebrafish methods cell and developmental biology |
| title_sub | cell and developmental biology |
| topic | Danio zébré - génétique Danio zébré - méthodes Zebra danio / Genetics / Research Zebra danios as laboratory animals / Laboratory manuals Zebrabärbling (DE-588)4079468-4 gnd Methode (DE-588)4038971-6 gnd |
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