HIV protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2009
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
485 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 457 S. Ill., graph. Darst. 27 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a HIV protocols |c ed. by Vinayaka R. Prasad and Ganjam V. Kalpana |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York, NY |b Humana Press |c 2009 | |
| 300 | |a XIV, 457 S. |b Ill., graph. Darst. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 485 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a AIDS (Disease) / Laboratory manuals | |
| 650 | 4 | |a HIV (Viruses) / Laboratory manuals | |
| 650 | 4 | |a HIV infections / Laboratory manuals | |
| 650 | 4 | |a HIV Infections / prevention & control | |
| 650 | 4 | |a Immunologic Techniques | |
| 650 | 4 | |a Molecular Biology / methods | |
| 650 | 4 | |a Virology / methods | |
| 650 | 4 | |a Clinical Protocols | |
| 650 | 2 | |a Biologie moléculaire - Méthode | |
| 650 | 7 | |a Biologie moléculaire |2 ram | |
| 650 | 7 | |a Diagnostic immunologique |2 ram | |
| 650 | 7 | |a Infections à HIV - Prévention |2 ram | |
| 650 | 2 | |a Infections à VIH - prévention et contrôle | |
| 650 | 2 | |a Protocoles cliniques | |
| 650 | 7 | |a Sida - Manuels de laboratoire |2 ram | |
| 650 | 7 | |a Virus de l'immunodéficience humaine - Manuels de laboratoire |2 ram | |
| 650 | 4 | |a AIDS (Disease) |v Laboratory manuals | |
| 650 | 4 | |a Clinical Protocols |v Laboratory Manuals | |
| 650 | 4 | |a HIV (Viruses) |v Laboratory manuals | |
| 650 | 4 | |a HIV Infections |x prevention & control | |
| 650 | 4 | |a HIV infections |v Laboratory manuals | |
| 650 | 4 | |a Immunologic Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Molecular Biology |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Virology |x methods |v Laboratory Manuals | |
| 700 | 1 | |a Prasad, Vinayaka R. |e Sonstige |0 (DE-588)13737321X |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 485 |w (DE-604)BV035362695 |9 485 | |
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Datensatz im Suchindex
| _version_ | 1804138508899581953 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Section I. Preparation of Virus Particles and Their
Analysis
1 Methods for Viral RNA Isolation and PCR Amplification for Sequencing
of Near Full-Length HIV-1 Genomes
Kimdar Sherefa Kemal, Milan Reinis, Barbara Weiser, and Harold Burger 3
2 Purification of HIV-1 Virions by Subtilisin Digestion or CD45
Immunoaffinity Depletion for Biochemical Studies
David E.Ott 15
3 Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method
Tong Goo, Immaculate Nankya, AwetAbraba, Ryan M. Troyer, Kenneth N. Nelson,
Andrea Rubio, and EricJ. Arts 27
Section II. Methods to Study HIV-1 Replication
Subsection A. Early Events
4 Cell-Free Assays for HIV-1 Uncoating
Christopher Aiken 41
5 Real-Time PCR Analysis of HIV-1 Replication Postentry Events
Jean L. Mbisa*, KristaA. Delviks-Frankenberrf, James A. Thomas,
Robert J. Gorelick, Vinay K. Pathak 55
6 Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells
Dibyakanti Mandal and Vinayaka R. Prasad 73
7 HIV-1 Recombination: An Experimental Assay and a Phylogenetic
Approach
Michael D. Moore, Mario PS. Chin, and Wei-Shau Hu 87
8 Methods of Preparation and Analysis of Intracellular Reverse Transcription
Complexes
Ariberto Fassati 107
9 Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription
Complexes by Co-immunoprecipitation
Sergey N. Iordanskiy and Michael I. Bukrinsky 121
10 Isolation and Analysis of HIV-1 Preintegration Complexes
Alan Engelman 135
11 Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy
Nathalie J. Arhel and Pierre Charneau 151
Subsection B. Late Events
12 Methods for the Study of HIV-1 Assembly
Abdul A. Waheed, Akira Ono, and Eric O. Freed 163
vii
viii Contents
13 Assembly of Immature HIV-1 Capsids
Using a Cell-Free System
Jaisri R. Lingappa and Beth K. Thielen 185
14 Preparation of Recombinant Hiv-1 Gag Protein and Assembly
of Virus-Like Particles In Vitro
Siddhartha A.K. Datta and Alan Rein 197
15 Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions
with Oligonucleotides
Andrew G. Stephen and Robert J. Fisher 209
16 Methods for Analysis of Incorporation and Annealing
oftRNALys in HIV-1
Shan Cen, Fei Guo, and Lawrence Kleiman 223
Section III. Specialized Approaches to Study HIV-1 Biology
AND PATHOGENESIS
17 Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors
Susana T. Valente and Stephen P. Goff. 235
18 Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi
Manuel Llano, Natassia Gaznick, and Eric M. Poeschla 257
19 Reverse Two-Hybrid Screening to Analyze Protein-Protein Interaction
of HIV-1 Viral and Cellular Proteins
Supratik Das and Gan jam V. Kalpana 271
20 Methods to Study Monocyte Migration Induced by HIV-Infected Cells
Vasudev R. Rao, Eliseo A. Eugenin, Joan W. Berman, and Vinayaka R. Prasad... .295
21 Novel Mouse Models for Understanding HIV-1 Pathogenesis
Aviva Joseph, Kaori Sanjjo, and Harris Goldstein 311
Section TV. Immunological Studies of HIV
Subsection A. Mucosal Immunology
22 Mucosal Antibody Responses to HIV
Zina Moldoveanu andjiri Mestecky 333
23 Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular
Immunology Assays
Barbara L. Shacklett, J. William Critchfield, Donna Lemongello 347
Subsectiom B. Measuring T Cell Responses via Flow Cytometry
24 Quantifying HTV-1 -Specific CD8+ T-Cell Responses Using ELISPOT
and Cytokine Flow Cytometry
Barbara L. Shacklett, J. William Critchfield, and Donna Lemongello 359
25 Multiparameter Flow Cytometry Monitoring of T Cell Responses
Holden T. Maecker 375
Subsection C. Antiviral Responses
26 Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
David C. Montefiori 395
27 Assessing the Antiviral Activity of HIV-1 -Specific Cytotoxic
T Lymphocytes
OttoO. Tans 407
Contents ix
28 Methods for Quantitating Antigen-Specific T Cell Responses Using
Functional Assays in Rhesus Macaques
Rama Rao Amara .417
Section V. Drug Resistant Viruses and Viral Evolution
29 Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture
Drug Selection
Maureen Oliveira, Bluma G. Brenner, and Mark A. Wainberg 427
30 Virus Evolution as a Tool to Study HFV-1 Biology
Ben Berkhout and Atze T. Das .435
Index 453
|
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| discipline | Medizin |
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| physical | XIV, 457 S. Ill., graph. Darst. 27 cm |
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| spelling | HIV protocols ed. by Vinayaka R. Prasad and Ganjam V. Kalpana 2. ed. New York, NY Humana Press 2009 XIV, 457 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 485 Includes bibliographical references and index AIDS (Disease) / Laboratory manuals HIV (Viruses) / Laboratory manuals HIV infections / Laboratory manuals HIV Infections / prevention & control Immunologic Techniques Molecular Biology / methods Virology / methods Clinical Protocols Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Protocoles cliniques Sida - Manuels de laboratoire ram Virus de l'immunodéficience humaine - Manuels de laboratoire ram AIDS (Disease) Laboratory manuals Clinical Protocols Laboratory Manuals HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Laboratory Manuals Molecular Biology methods Laboratory Manuals Virology methods Laboratory Manuals Prasad, Vinayaka R. Sonstige (DE-588)13737321X oth Methods in molecular biology 485 (DE-604)BV035362695 485 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017043747&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | HIV protocols Methods in molecular biology AIDS (Disease) / Laboratory manuals HIV (Viruses) / Laboratory manuals HIV infections / Laboratory manuals HIV Infections / prevention & control Immunologic Techniques Molecular Biology / methods Virology / methods Clinical Protocols Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Protocoles cliniques Sida - Manuels de laboratoire ram Virus de l'immunodéficience humaine - Manuels de laboratoire ram AIDS (Disease) Laboratory manuals Clinical Protocols Laboratory Manuals HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Laboratory Manuals Molecular Biology methods Laboratory Manuals Virology methods Laboratory Manuals |
| title | HIV protocols |
| title_auth | HIV protocols |
| title_exact_search | HIV protocols |
| title_full | HIV protocols ed. by Vinayaka R. Prasad and Ganjam V. Kalpana |
| title_fullStr | HIV protocols ed. by Vinayaka R. Prasad and Ganjam V. Kalpana |
| title_full_unstemmed | HIV protocols ed. by Vinayaka R. Prasad and Ganjam V. Kalpana |
| title_short | HIV protocols |
| title_sort | hiv protocols |
| topic | AIDS (Disease) / Laboratory manuals HIV (Viruses) / Laboratory manuals HIV infections / Laboratory manuals HIV Infections / prevention & control Immunologic Techniques Molecular Biology / methods Virology / methods Clinical Protocols Biologie moléculaire - Méthode Biologie moléculaire ram Diagnostic immunologique ram Infections à HIV - Prévention ram Infections à VIH - prévention et contrôle Protocoles cliniques Sida - Manuels de laboratoire ram Virus de l'immunodéficience humaine - Manuels de laboratoire ram AIDS (Disease) Laboratory manuals Clinical Protocols Laboratory Manuals HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Laboratory Manuals Molecular Biology methods Laboratory Manuals Virology methods Laboratory Manuals |
| topic_facet | AIDS (Disease) / Laboratory manuals HIV (Viruses) / Laboratory manuals HIV infections / Laboratory manuals HIV Infections / prevention & control Immunologic Techniques Molecular Biology / methods Virology / methods Clinical Protocols Biologie moléculaire - Méthode Biologie moléculaire Diagnostic immunologique Infections à HIV - Prévention Infections à VIH - prévention et contrôle Protocoles cliniques Sida - Manuels de laboratoire Virus de l'immunodéficience humaine - Manuels de laboratoire AIDS (Disease) Laboratory manuals Clinical Protocols Laboratory Manuals HIV (Viruses) Laboratory manuals HIV Infections prevention & control HIV infections Laboratory manuals Immunologic Techniques Laboratory Manuals Molecular Biology methods Laboratory Manuals Virology methods Laboratory Manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017043747&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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