DNA methylation: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2009
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
507 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XVI, 391 S. Ill., graph. Darst. |
| ISBN: | 9781617379383 9781934115619 |
Internformat
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| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York |b Humana Press |c 2009 | |
| 300 | |a XVI, 391 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Methods in molecular biology |v 507 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Literaturangaben | ||
| 650 | 4 | |a DNA / Methylation | |
| 650 | 4 | |a DNA Methylation | |
| 650 | 7 | |a ADN - Méthylation |2 ram | |
| 650 | 2 | |a Méthylation de l'ADN | |
| 650 | 4 | |a DNA Methylation | |
| 650 | 4 | |a DNA |x Methylation | |
| 650 | 0 | 7 | |a DNS |0 (DE-588)4070512-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methylierung |0 (DE-588)4123751-1 |2 gnd |9 rswk-swf |
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| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Tost, Jörg |e Sonstige |4 oth | |
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Datensatz im Suchindex
| _version_ | 1804138508841910272 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
Part I. Introduction
1. DNA Methylation: An Introduction to the Biology
and the Disease-Associated Changes of a Promising Biomarker
Jb rg Tost 3
Part II. Global Methylation Levels
2. Quantification of Global DNA Methylation by Capillary Electrophoresis
and Mass Spectrometry
Maria Berdasco, Mario F. Fraga, and Manel Esteller 23
3. Methyl Group Acceptance Assay for the Determination of Global DNA
Methylation Levels
Kenneth P. Nephew, Curt Batch, and David G. Skalnik 35
Part III. Methods for Whole Genome Analysis of DNA
Methylation Patterns
4. Immunodetection Array
Johannes Prb ll, Christian Wechselberger, Mathilde Fbdermayr, Otto Zach,
and Dieter Lutz 45
5. Methylated DNA Immunoprecipitation (MeDIP)
Fabio Mohn, Michael Weber, Dirk Schiibeler, and Tim-Christoph Roloff 55
6. The MIRA Method for DNA Methylation Analysis
TiborA. Ranch and Gerd P. Pfeifer 65
7. The HELP Assay
Mayumi Oda and John M. Greatly 77
8. Differential Methylation Hybridization: Profiling DNA Methylation
with a High-Density CpG Island Microarray
Pearlly S. Tan, Dustin Potter, Daniel E. Deatberage, Shili Lin,
and Tim H.-M. Huang 89
9. Analysis of DNA Methylation by Amplification
of Intermethylated Sites (AIMS)
Mireia Jordd, Jairo Rodriguez, Jordi Frigola, and Miguel A. Peinado 107
10. Methylation-Sensitive Representational Difference Analysis (MS-RDA)
Toshikazu Ushijima and Satoshi Yamashita 117
ix
x Contents
11. Restriction Landmark Genomic Scanning: Analysis of CpG Islands
in Genomes by 2D Gel Electrophoresis
Joseph F. Costello, Dominic J. Smiraglia, Chibo Hong,
and Christoph Plass 131
12. GoldenGate® Assay for DNA Methylation Profiling
Marina Bibikova and Jian-Bing Fan 149
13. 5 -Azacytidine Expression Arrays
Paul Cairns 165
Part IV. Gene-Specific Methylation Analysis
14. DNA Methylation Analysis by Bisulfite Conversion, Cloning,
and Sequencing of Individual Clones
Yingying Zhang, Christian Rohde, Sascha Tierling,
Heinrich Stamerjohanns, Richard Reinhardt, Jorn Walter,
and Albert Jeltsch 177
15. Identification and Quantification of Differentially Methylated Loci
by the Pyrosequencing™ Technology
Emelyne Dejeux, Hafida El abdalaoui, Ivo Glynne Gut, andjbrg Tost 189
16. Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific
Cleavage and Primer Extension Methods
Dirk van den Boom and Mathias Ehrich 207
17. Melting Curve Assays for DNA Methylation Analysis
TomaszK. Wojdacz and Alexander Dobrovic 229
18. Methylation SNaPshot: A Method for the Quantification
of Site-Specific DNA Methylation Levels
Zachary Kaminsky andArturas Petronis 241
19. Bio-COBRA: Absolute Quantification of DNA Methylation
in Electrofluidics Chips
Romulo Martin Brena and Christoph Plass 257
20. Restriction Digestion and Real-Time PCR (qAMP)
Christopher C. Oakes, Sophie La Salle, Jacquetta M. Trasler,
and Bernard Robaire 271
21. MethylQuant: A Real-Time PCR-Based Method to Quantify DNA
Methylation at Single Specific Cytosines
Claire Dugast-Darzacq and Thierry Grange 281
22. Methylation-Specific PCR
Julien D. F. Licchesi and James G. Herman 305
23. MethyLight
Mihaela Campan, Daniel J. Weisenberger, Binh Trinh,
and Peter W. Laird 325
24. Quantification of Methylated DNA by HeavyMethyl Duplex PCR
Jttrgen Distler 339
Contents xi
Part V. Special Applications
25 Analysis of Methylated Circulating DNA in Cancer Patients Blood
Eiji Sunami, Anh-Thu Vu, Sandy L. Nguyen, and Dave S. B. Hoon 349
26 Prevention of PCR Cross-Contamination by UNG Treatment
of Bisulfite-Treated DNA
Reimo Tetzner 357
27 Profiling DNA Methylation from Small Amounts of Genomic DNA
Starting Material: Efficient Sodium Bisulfite Conversion
and Subsequent Whole-Genome Amplification
Jonathan Mill and Arturas Petronis 371
Index 383
|
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| isbn | 9781617379383 9781934115619 |
| language | English |
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| publishDate | 2009 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | DNA methylation methods and protocols ed. by Jörg Tost 2. ed. New York Humana Press 2009 XVI, 391 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 507 Springer protocols Literaturangaben DNA / Methylation DNA Methylation ADN - Méthylation ram Méthylation de l'ADN DNS (DE-588)4070512-2 gnd rswk-swf Methylierung (DE-588)4123751-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content DNS (DE-588)4070512-2 s Methylierung (DE-588)4123751-1 s b DE-604 Tost, Jörg Sonstige oth Methods in molecular biology 507 (DE-604)BV035362695 507 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017043710&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA methylation methods and protocols Methods in molecular biology DNA / Methylation DNA Methylation ADN - Méthylation ram Méthylation de l'ADN DNS (DE-588)4070512-2 gnd Methylierung (DE-588)4123751-1 gnd |
| subject_GND | (DE-588)4070512-2 (DE-588)4123751-1 (DE-588)4143413-4 |
| title | DNA methylation methods and protocols |
| title_auth | DNA methylation methods and protocols |
| title_exact_search | DNA methylation methods and protocols |
| title_full | DNA methylation methods and protocols ed. by Jörg Tost |
| title_fullStr | DNA methylation methods and protocols ed. by Jörg Tost |
| title_full_unstemmed | DNA methylation methods and protocols ed. by Jörg Tost |
| title_short | DNA methylation |
| title_sort | dna methylation methods and protocols |
| title_sub | methods and protocols |
| topic | DNA / Methylation DNA Methylation ADN - Méthylation ram Méthylation de l'ADN DNS (DE-588)4070512-2 gnd Methylierung (DE-588)4123751-1 gnd |
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