Auditory and vestibular research: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J. [u.a.]
Humana
2009
|
| Schriftenreihe: | Methods in molecular biology
493 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XII, 421 S. ill. (some col.) 27 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Auditory and vestibular research |b methods and protocols |c edited by Bernd Sokolowski |
| 264 | 1 | |a Totowa, N.J. [u.a.] |b Humana |c 2009 | |
| 300 | |a XII, 421 S. |b ill. (some col.) |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 493 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Vestibular apparatus | |
| 650 | 4 | |a Hearing disorders | |
| 650 | 4 | |a Equilibrium (Physiology) | |
| 650 | 4 | |a Auditory Pathways / physiology | |
| 650 | 4 | |a Ear Diseases / diagnosis | |
| 650 | 4 | |a Vestibule, Labyrinth / physiology | |
| 650 | 4 | |a Vestibular Diseases / diagnosis | |
| 650 | 7 | |a Oreille interne |2 ram | |
| 650 | 7 | |a Vestibule du labyrinthe osseux |2 ram | |
| 650 | 4 | |a Auditory Pathways |x physiology | |
| 650 | 4 | |a Ear Diseases |x diagnosis | |
| 650 | 4 | |a Equilibrium (Physiology) | |
| 650 | 4 | |a Hearing disorders | |
| 650 | 4 | |a Vestibular Diseases |x diagnosis | |
| 650 | 4 | |a Vestibular apparatus | |
| 650 | 4 | |a Vestibule, Labyrinth |x physiology | |
| 700 | 1 | |a Sokolowski, Bernd |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 493 |w (DE-604)BV035362695 |9 493 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017002699&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017002699 | ||
Datensatz im Suchindex
| _version_ | 1804138446687567872 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I. Nucleic Acid Protocols
1 RNA Isolation from Xenopus Inner Ear Sensory Endorgans
for Transcriptional Profiling and Molecular Cloning
Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier,
and Elba E. Serrano 3
2 Synthesis of Biotin-Labeled RNA for Gene Expression Measurements
Using Oligonucleotide Arrays
Ana E. Vazquez, Liping Nie, and Ebenezer N. Yamoah 21
3 In situ Hybridization Approach to Study mRNA Expression
and Distribution in Cochlear Frozen Sections
Hakim Hiel 31
4 Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal
Identification
Takunori Satoh and Donna M. Fekete 47
5 Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic
Origins of the Cochlear Nucleus
Jun Chul Kim and Susan M. Dymecki 65
6 The Practical Use of Cre and loxP Technologies in Mouse Auditory
Research
Tiling Yu andjian Zuo 87
7 Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory
Epithelium
Inna A. Belyantseva 103
8 Electroporation-Mediated Gene Transfer to the Developing Mouse
Inner Ear
John V. Brigande, Samuel P. Gubbels, David W. Woessner,
Jonathan J. Jungwirth, and Catherine S. Bresee 125
9 Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear
Kazuo Oshima, Pascal Senn, and Stefan Heller 141
10 Molecular Biology of Vestibular Schwannomas
Long-Sheng Chang and D. Bradley Welling 163
11 Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis
Media Causing Pathogens
Jonathan C. Thomas and Melinda M. Pettigrew 179
12 Fluorescence In Situ Hybridization for the Detection of Biofilm
in the Middle Ear and Upper Respiratory Tract Mucosa
Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-StoodUy,
Joseph E. Kerschner, and Garth D. Ehrlich J91
vii
viii Contents
13 Positional Cloning of Deafness Genes
Hannie Kretner and Brans P.M. Cremers 215
Part II. Amino Acid Protocols
14 Twist- Off Purification of Hair Bundles
Jung-Bum Shin, James Pagana, and Peter G. Gillespie 241
15 Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions
in the Auditory System
Dhasakumar S. Navaratnam 257
16 The Use of 2-D Gels to Identify Novel Protein-Protein Interactions
in the Cochlea
Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H. A. Sokolowski 269
17 Identification of Functionally Important Residues/Domains in Membrane
Proteins Using an Evolutionary Approach Coupled with Systematic
Mutational Analysis
Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge,
and William E. Brownell 287
18 In Vivo Verification of Protein Interactions in the Inner Ear
by Coimmunoprecipitation
Margaret C. Harvey and Bernd H. A. Sokolowski 299
19 Identification of Transcription Factor-DNA Interactions Using Chromatin
Immunoprecipitation Assays
Liping Nie, Ana E. Vazquez, and Ebenezer N. Yatnoah 311
20 Surface Plasmon Resonance (SPR) Analysis of Binding Interactions
of Proteins in Inner-Ear Sensory Epithelia
Dennis G. Drescher, Neeliyath A. Bjimakrishnan, Marian J. Drescher 323
21 Multiplexed Isobaric Tagging Protocols for Quantitative Mass
Spectrometry Approaches to Auditory Research
Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan 345
Part III. Imaging Protocols
22 Fluorescence Microscopy Methods in the Study of Protein Structure
and Function
Heather Jensen-Smith, Benjamin Currall, Danielle Rossino, LeAnn Tiede,
Michael Nichols, and Richard Hallworth 369
23 Ion Imaging in the Cochlear Hair Cells
Gregory I. Frolenkov 381
24 Atomic Force Microscopy in Studies of the Cochlea
Michio Murakoshi and Hiroshi Wada .401
Index 415
|
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| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T21:28:19Z |
| institution | BVB |
| language | English |
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| physical | XII, 421 S. ill. (some col.) 27 cm |
| publishDate | 2009 |
| publishDateSearch | 2009 |
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| publisher | Humana |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Auditory and vestibular research methods and protocols edited by Bernd Sokolowski Totowa, N.J. [u.a.] Humana 2009 XII, 421 S. ill. (some col.) 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 493 Includes bibliographical references and index Vestibular apparatus Hearing disorders Equilibrium (Physiology) Auditory Pathways / physiology Ear Diseases / diagnosis Vestibule, Labyrinth / physiology Vestibular Diseases / diagnosis Oreille interne ram Vestibule du labyrinthe osseux ram Auditory Pathways physiology Ear Diseases diagnosis Vestibular Diseases diagnosis Vestibule, Labyrinth physiology Sokolowski, Bernd Sonstige oth Methods in molecular biology 493 (DE-604)BV035362695 493 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017002699&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Auditory and vestibular research methods and protocols Methods in molecular biology Vestibular apparatus Hearing disorders Equilibrium (Physiology) Auditory Pathways / physiology Ear Diseases / diagnosis Vestibule, Labyrinth / physiology Vestibular Diseases / diagnosis Oreille interne ram Vestibule du labyrinthe osseux ram Auditory Pathways physiology Ear Diseases diagnosis Vestibular Diseases diagnosis Vestibule, Labyrinth physiology |
| title | Auditory and vestibular research methods and protocols |
| title_auth | Auditory and vestibular research methods and protocols |
| title_exact_search | Auditory and vestibular research methods and protocols |
| title_full | Auditory and vestibular research methods and protocols edited by Bernd Sokolowski |
| title_fullStr | Auditory and vestibular research methods and protocols edited by Bernd Sokolowski |
| title_full_unstemmed | Auditory and vestibular research methods and protocols edited by Bernd Sokolowski |
| title_short | Auditory and vestibular research |
| title_sort | auditory and vestibular research methods and protocols |
| title_sub | methods and protocols |
| topic | Vestibular apparatus Hearing disorders Equilibrium (Physiology) Auditory Pathways / physiology Ear Diseases / diagnosis Vestibule, Labyrinth / physiology Vestibular Diseases / diagnosis Oreille interne ram Vestibule du labyrinthe osseux ram Auditory Pathways physiology Ear Diseases diagnosis Vestibular Diseases diagnosis Vestibule, Labyrinth physiology |
| topic_facet | Vestibular apparatus Hearing disorders Equilibrium (Physiology) Auditory Pathways / physiology Ear Diseases / diagnosis Vestibule, Labyrinth / physiology Vestibular Diseases / diagnosis Oreille interne Vestibule du labyrinthe osseux Auditory Pathways physiology Ear Diseases diagnosis Vestibular Diseases diagnosis Vestibule, Labyrinth physiology |
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