Verfügbarkeit: Membrane proteomics

Membrane proteomics: methods and protocols

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Format:	Buch
Sprache:	English
Veröffentlicht:	New York, NY Humana 2009
Schriftenreihe:	Methods in molecular biology 528
Schlagworte:	Protéines membranaires - analyse Membrane Proteins > analysis Membrane proteins Proteomics Proteomics > methods Labortechnik Membranproteine
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIII, 342 S. Ill., graph. Darst.
ISBN:	9781603273091 9781603273107

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MARC


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Datensatz im Suchindex

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adam_text	Contents Preface ...................................................................... ρ Contributors ................................................................ xi Part I. In Silico Methods for Prediction oe Membrane Protein Hydrophobicity and Topology 1 Online Tools for Predicting Integral Membrane Proteins Henry Bigelow and Burkhard Rost............................................ З 2 In Silico Identification of Novel G Protein Coupled Receptors Matthew N. Davies and Darren R. Flower ..................................... 25 3 Transcriptome-Based Identification of Candidate Membrane Proteins Edward J. Evans, Lawrence Непе, Mai Vtwng, S. Hussain I. Abidi, and Simon J. Davis ........................................................ 37 Part II. Extraction and Purification of Membrane Proteins Part A. Plant Membrane Proteins 4 Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrirugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis Gian Maria D Amici, Christian G. Huber, and Letto Zolla..................... 61 Part В. Prokaryotic Membrane Proteins 5 Extraction of Yeast Mitochondria! Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning Henrik Everberß, Niklas Gustavsson, and Folke Tjerneld ........................ 73 6 ló-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis RalfJ. Braun, Norbert Kinkl, Hans Zischka, and Marius Ueffinß............... 83 Part C. Mammalian Membrane Proteins 7 Sequential Detergent Extraction Prior to Mass Spectrometry Analysis Fiona M. McCarthy, Amanda M. Cooksey, and Shane С Burgess ................. Ill 8 Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning Jens Schindler and Hans Gerd Nothwanß...................................... 119 VII v¡¡¡ Contents 9 Protocol to Enrich and Analyze Plasma Membrane Proteins Jacek R. Wiśniewski .......................................................... 127 10 Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing Matthew J. Peirce, Andrew P. Cope, and Robin Wait ............................ 135 11 Identification of Target Membrane Proteins as Detected by Phage Antibodies Cecile A. W. Geuijen, Ar jen Q. Bakker, and John de Kruif. ...................... 141 12 Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates Georgina S. Butler, Richard A. Dean, Derek Smith, ■ Christopher M. Overall ................................................ 159 13 Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction Robert J.A. Goode and Richard J. Simpson ..................................... 177 14 Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics Leonard J. Foster ............................................................. 189 15 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification Nadia Korfali, Elizabeth A. L. Fairley, Selene K. Swanson, Laurence Florens, and Eric C. Schirmer ...................................................... 201 16 Isolation of Extracellular Membranous Vesicles for Proteomic Analysis Rommel A. Mathias, Justin W. Lim, Honjfji, and Richard J. Simpson ...........227 Part III. Separation of Membrane Proteins 17 Enrichment of Human Platelet Membranes for Proteomic Analysis David W. Greening, Kristen M. Glenister, Rosemary L. Sparrow, and Richard J. Simpson ....................................................245 18 Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis Thierry Rabilloud ........................................................... 259 19 Two-Dimensional Separation of Membrane Proteins by ló-BAC-SDS-PAGE Hans Gerd Nathwang and Jens Schindler...................................... 269 Part IV. Identification and Quantification of Membrane Proteins 20 MudPIT Analysis: Application to Human Heart Tissue Kelti G, Kline and Christine CWu ........................................... 281 Contents ix 21 Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis Nan Wang,]. Bryce Тоищ and Ілащ Li ...................................... 295 22 Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry SrijeetK. Mitra- and Michael В. Goshe ......................................... 311 23 Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins Xuequn Chen and Philip С Andrews ......................................... 327 Index ....................................................................... 339
adam_txt	Contents Preface . ρ Contributors . xi Part I. In Silico Methods for Prediction oe Membrane Protein Hydrophobicity and Topology 1 Online Tools for Predicting Integral Membrane Proteins Henry Bigelow and Burkhard Rost. З 2 In Silico Identification of Novel G Protein Coupled Receptors Matthew N. Davies and Darren R. Flower . 25 3 Transcriptome-Based Identification of Candidate Membrane Proteins Edward J. Evans, Lawrence Непе, Mai Vtwng, S. Hussain I. Abidi, and Simon J. Davis . 37 Part II. Extraction and Purification of Membrane Proteins Part A. Plant Membrane Proteins 4 Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrirugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis Gian Maria D'Amici, Christian G. Huber, and Letto Zolla. 61 Part В. Prokaryotic Membrane Proteins 5 Extraction of Yeast Mitochondria! Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning Henrik Everberß, Niklas Gustavsson, and Folke Tjerneld . 73 6 ló-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis RalfJ. Braun, Norbert Kinkl, Hans Zischka, and Marius Ueffinß. 83 Part C. Mammalian Membrane Proteins 7 Sequential Detergent Extraction Prior to Mass Spectrometry Analysis Fiona M. McCarthy, Amanda M. Cooksey, and Shane С Burgess . Ill 8 Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning Jens Schindler and Hans Gerd Nothwanß. 119 VII v¡¡¡ Contents 9 Protocol to Enrich and Analyze Plasma Membrane Proteins Jacek R. Wiśniewski . 127 10 Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing Matthew J. Peirce, Andrew P. Cope, and Robin Wait . 135 11 Identification of Target Membrane Proteins as Detected by Phage Antibodies Cecile A. W. Geuijen, Ar jen Q. Bakker, and John de Kruif. . 141 12 Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates Georgina S. Butler, Richard A. Dean, Derek Smith, '■ Christopher M. Overall . 159 13 Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction Robert J.A. Goode and Richard J. Simpson . 177 14 Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics Leonard J. Foster . 189 15 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification Nadia Korfali, Elizabeth A. L. Fairley, Selene K. Swanson, Laurence Florens, and Eric C. Schirmer . 201 16 Isolation of Extracellular Membranous Vesicles for Proteomic Analysis Rommel A. Mathias, Justin W. Lim, Honjfji, and Richard J. Simpson .227 Part III. Separation of Membrane Proteins 17 Enrichment of Human Platelet Membranes for Proteomic Analysis David W. Greening, Kristen M. Glenister, Rosemary L. Sparrow, and Richard J. Simpson .245 18 Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis Thierry Rabilloud . 259 19 Two-Dimensional Separation of Membrane Proteins by ló-BAC-SDS-PAGE Hans Gerd Nathwang and Jens Schindler. 269 Part IV. Identification and Quantification of Membrane Proteins 20 MudPIT Analysis: Application to Human Heart Tissue Kelti G, Kline and Christine CWu . 281 Contents ix 21 Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis Nan Wang,]. Bryce Тоищ and Ілащ Li . 295 22 Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry SrijeetK. Mitra- and Michael В. Goshe . 311 23 Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins Xuequn Chen and Philip С Andrews . 327 Index . 339
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spelling	Membrane proteomics methods and protocols ed. by Matthew J. Peirce ... New York, NY Humana 2009 XIII, 342 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 528 Springer-Protocols Protéines membranaires - analyse Membrane Proteins analysis Membrane proteins Proteomics Proteomics methods Labortechnik (DE-588)4123602-6 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 s Labortechnik (DE-588)4123602-6 s b DE-604 Peirce, Matthew J. Sonstige oth Methods in molecular biology 528 (DE-604)BV035362695 528 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016998385&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Membrane proteomics methods and protocols Methods in molecular biology Protéines membranaires - analyse Membrane Proteins analysis Membrane proteins Proteomics Proteomics methods Labortechnik (DE-588)4123602-6 gnd Membranproteine (DE-588)4130026-9 gnd
subject_GND	(DE-588)4123602-6 (DE-588)4130026-9
title	Membrane proteomics methods and protocols
title_auth	Membrane proteomics methods and protocols
title_exact_search	Membrane proteomics methods and protocols
title_exact_search_txtP	Membrane proteomics methods and protocols
title_full	Membrane proteomics methods and protocols ed. by Matthew J. Peirce ...
title_fullStr	Membrane proteomics methods and protocols ed. by Matthew J. Peirce ...
title_full_unstemmed	Membrane proteomics methods and protocols ed. by Matthew J. Peirce ...
title_short	Membrane proteomics
title_sort	membrane proteomics methods and protocols
title_sub	methods and protocols
topic	Protéines membranaires - analyse Membrane Proteins analysis Membrane proteins Proteomics Proteomics methods Labortechnik (DE-588)4123602-6 gnd Membranproteine (DE-588)4130026-9 gnd
topic_facet	Protéines membranaires - analyse Membrane Proteins analysis Membrane proteins Proteomics Proteomics methods Labortechnik Membranproteine
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