Light and video microscopy:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier Acad. Press
2009
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 289 S., [2] Bl. Ill., graph. Darst. |
| ISBN: | 9780123742346 |
Internformat
MARC
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| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier Acad. Press |c 2009 | |
| 300 | |a XII, 289 S., [2] Bl. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804138118216941568 |
|---|---|
| adam_text | Contents
Preface
1.
The Relation between the Object
and the Image
Luminous and Nonluminous Objects
Object and Image
Theories of Vision
Light Travels in Straight Lines
Images Formed in a Camera
Obscura-.
Geometric
Considerations
Where Does Light Come From?
How Can the Amount of Light Be Measured?
2.
The Geometric Relationship between
Object and Image
Reflection by a Plane Mirror
Reflection by a Curved Mirror
Reflection from Various Sources
Images Formed by Refraction at a Plane Surface
Images Formed by Refraction at a Curved Surface
Fermat s Principle
Optical Path Length
Lens Aberrations
Geometric Optics and Biology
Geometric Optics of the Human Eye
Web Resources
3.
The Dependence of Image Formation
on the Nature Of Light
xi 4.
Bright-Field Microscopy
Components of the Microscope
The Optical Paths of the Light Microscope
Using the Bright-Field Microscope
Depth of Field
Out-of-Focus Contrast
Uses of Bright-Field Microscopy
Care and Cleaning of the Light Microscope
Web Resources
I
I
2
5
5
8
10
11
II
13
16
16
20
26
28
29
31
31
33
35
Christiaan Huygens and the Invention of the
Wave Theory of Light
35
Thomas Young and the Development of the Wave
Theory of Light
39
lames Clerk Maxwell and the Wave Theory of Light
51
Ernst Abbe and the Relationship of Diffraction to
Image Formation
53
Resolving Power and the Limit of Resolution
60
Contrast
63
Web Resources
65
5.
Photomicrography
Setting up the Microscope for Photomicrography
Scientific History of Photography
General Nature of the Photographic Process
The Resolution of the Film
Exposure and Composition
The Similarities between Film and the Retina
Web Resources
6.
Methods of Generating Contrast
Dark-Field Microscopy
Rheinberg
Illumination
Oblique Illumination
Phase-Contrast Microscopy
Oblique Illumination Reconsidered
Annular Illumination
7.
Polarization Microscopy
What Is Polarized Light?
Use an Analyzer to Test for Polarized Light
Production of Polarized Light
Influencing Light
Design of a Polarizing Microscope
What Is the Molecular Basis of Birefringence?
Interference of Polarized Light
The Origin of Colors in
Biréfringent
Specimens
Use of Compensators to Determine the
Magnitude and Sign of Birefringence
Crystalline versus Form Birefringence
67
68
73
76
79
80
80
80
81
83
83
85
87
90
91
93
93
95
95
97
98
99
108
109
111
111
113
114
119
120
122
126
133
133
144
vii
viii
Contents
Orthoscopic
versus
Conoscopic
Observations
1
45
Reflected Light Polarization Microscopy
145
Uses of Polarization Microscopy
145
Optical Rotatory (or Rotary) Polarization and
Optical Rotatory (or Rotary) Dispersion
147
Web Resources
148
8.
Interference Microscopy
149
Generation of Interference Colors
149
The Relationship of Interference Microscopy
to Phase-Contrast Microscopy
152
Quantitative Interference Microscopy:
Determination of the Refractive Index, Mass,
Concentration of Dry Matter, Concentration of
Water, and Density
154
Source of Errors When Using an Interference
Microscope
156
Making a Coherent Reference Beam
157
Double-Beam versus Multiple-Beam
Interference
158
Interference Microscopes Based on a Mach-
Zehnder Type Interferometer
158
Interference Microscopes Based on Polarized
Light
160
The Use of Transmission Interference
Microscopy in Biology
164
Reflection-Interference Microscopy
164
Uses of Reflection-Interference Microscopy
in Biology
165
9.
Differential Interference Contrast
(DIC) Microscopy
167
Design of a Transmitted Light Differential
Interference Contrast Microscope
167
Interpretation of a Transmitted Light
Differential Interference Contrast Image
169
Design of a Reflected Light Differential
Interference Contrast Microscope
173
Interpretation of a Reflected Light Differential
Interference Contrast Image
174
10.
Amplitude Modulation Contrast
Microscopy
Fluorescence Microscopy
Discovery of Fluorescence
Physics of Fluorescence
Design of a Fluorescence Microscope
175
Hoffman Modulation Contrast Microscopy
Reflected Light Hoffman Modulation Contrast
Microscopy 1
79
The Single-Sideband Edge Enhancement
Microscope
179
183
183
184
190
Fluorescence Probes
192
Pitfalls and Cures in Fluorescence Microscopy
193
Web Resources
193
12.
Various Types of Microscopes and
Accessories
195
Confocal Microscopes
195
Laser Microbeam Microscope
198
Optical Tweezers
199
Laser Capture
Microdissection
199
Laser
Doppler
Microscope
200
Centrifuge Microscope
200
Х
-Ray Microscope
201
Infrared Microscope
202
Nuclear Magnetic Resonance Imaging
Microscope
202
Stereo Microscopes
202
Scanning Probe Microscopes
203
Acoustic Microscope
204
Horizontal and Traveling Microscopes
205
Microscopes for Children
205
Microscope Accessories
205
Web Resources
206
13.
Video and Digital Microscopy
207
The Value of Video and Digital Microscopy
207
Video and Digital Cameras: The Optical to
Electrical Signal Converters
208
Monitors: Conversion of an Electronic Signal
14.
into an Optical Signal
216
Storage of Video and Digital Images
217
Connecting a Video System
218
Web Resources
218
Image Processing and Analysis
219
Analog Image Processing
219
Digital Image Processing
224
Enhancement Functions of Digital Image
Processors
225
Analysis Functions of Digital Image Processors
228
The Ethics of Digital Image Processing
229
Web Resources
230
176 15.
Laboratory Exercises
231
Laboratory
1 :
The Nature of Light and
Geometric Optics
231
Laboratory
2:
Physical Optics
233
Laboratory
3:
The Bright-Field Microscope
and Image Formation
234
Laboratory
4:
Phase-Contrast Microscopy,
Dark-Field Microscopy,
Rheinberg
Illumination, and Oblique Illumination
236
Laboratory
5:
Fluorescence Microscopy
239
Contents
ix
Laboratory
6:
Polarized Light
240
Laboratory
7:
Polarizing Light Microscopy
241
Laboratory
8:
Interference Microscopy
241
Laboratory
9:
Differential Interference
Contrast Microscopy and Hoffman Modulation
Contrast Microscopy
242
Laboratory
10:
Video and Digital Microscopy
and Analog and Digital Image Processing
243
Commercial Sources for Laboratory Equipment
and Specimens
245
References
247
Appendix I. A Final Exam
273
Appendix II. A Microscopist s Model of
the Photon
277
Index
285
Visit our companion website for additional book content, including answers to the final exam in the book, all of the images
from the book, and additional color images HYPERLINK http://www.elsevierdirect.com/companions/9780123742346
www.elsevierdirect.com/companions/9780123742346
|
| adam_txt |
Contents
Preface
1.
The Relation between the Object
and the Image
Luminous and Nonluminous Objects
Object and Image
Theories of Vision
Light Travels in Straight Lines
Images Formed in a Camera
Obscura-.
Geometric
Considerations
Where Does Light Come From?
How Can the Amount of Light Be Measured?
2.
The Geometric Relationship between
Object and Image
Reflection by a Plane Mirror
Reflection by a Curved Mirror
Reflection from Various Sources
Images Formed by Refraction at a Plane Surface
Images Formed by Refraction at a Curved Surface
Fermat's Principle
Optical Path Length
Lens Aberrations
Geometric Optics and Biology
Geometric Optics of the Human Eye
Web Resources
3.
The Dependence of Image Formation
on the Nature Of Light
xi 4.
Bright-Field Microscopy
Components of the Microscope
The Optical Paths of the Light Microscope
Using the Bright-Field Microscope
Depth of Field
Out-of-Focus Contrast
Uses of Bright-Field Microscopy
Care and Cleaning of the Light Microscope
Web Resources
I
I
2
5
5
8
10
11
II
13
16
16
20
26
28
29
31
31
33
35
Christiaan Huygens and the Invention of the
Wave Theory of Light
35
Thomas Young and the Development of the Wave
Theory of Light
39
lames Clerk Maxwell and the Wave Theory of Light
51
Ernst Abbe and the Relationship of Diffraction to
Image Formation
53
Resolving Power and the Limit of Resolution
60
Contrast
63
Web Resources
65
5.
Photomicrography
Setting up the Microscope for Photomicrography
Scientific History of Photography
General Nature of the Photographic Process
The Resolution of the Film
Exposure and Composition
The Similarities between Film and the Retina
Web Resources
6.
Methods of Generating Contrast
Dark-Field Microscopy
Rheinberg
Illumination
Oblique Illumination
Phase-Contrast Microscopy
Oblique Illumination Reconsidered
Annular Illumination
7.
Polarization Microscopy
What Is Polarized Light?
Use an Analyzer to Test for Polarized Light
Production of Polarized Light
Influencing Light
Design of a Polarizing Microscope
What Is the Molecular Basis of Birefringence?
Interference of Polarized Light
The Origin of Colors in
Biréfringent
Specimens
Use of Compensators to Determine the
Magnitude and Sign of Birefringence
Crystalline versus Form Birefringence
67
68
73
76
79
80
80
80
81
83
83
85
87
90
91
93
93
95
95
97
98
99
108
109
111
111
113
114
119
120
122
126
133
133
144
vii
viii
Contents
Orthoscopic
versus
Conoscopic
Observations
1
45
Reflected Light Polarization Microscopy
145
Uses of Polarization Microscopy
145
Optical Rotatory (or Rotary) Polarization and
Optical Rotatory (or Rotary) Dispersion
147
Web Resources
148
8.
Interference Microscopy
149
Generation of Interference Colors
149
The Relationship of Interference Microscopy
to Phase-Contrast Microscopy
152
Quantitative Interference Microscopy:
Determination of the Refractive Index, Mass,
Concentration of Dry Matter, Concentration of
Water, and Density
154
Source of Errors When Using an Interference
Microscope
156
Making a Coherent Reference Beam
157
Double-Beam versus Multiple-Beam
Interference
158
Interference Microscopes Based on a Mach-
Zehnder Type Interferometer
158
Interference Microscopes Based on Polarized
Light
160
The Use of Transmission Interference
Microscopy in Biology
164
Reflection-Interference Microscopy
164
Uses of Reflection-Interference Microscopy
in Biology
165
9.
Differential Interference Contrast
(DIC) Microscopy
167
Design of a Transmitted Light Differential
Interference Contrast Microscope
167
Interpretation of a Transmitted Light
Differential Interference Contrast Image
169
Design of a Reflected Light Differential
Interference Contrast Microscope
173
Interpretation of a Reflected Light Differential
Interference Contrast Image
174
10.
Amplitude Modulation Contrast
Microscopy
Fluorescence Microscopy
Discovery of Fluorescence
Physics of Fluorescence
Design of a Fluorescence Microscope
175
Hoffman Modulation Contrast Microscopy
Reflected Light Hoffman Modulation Contrast
Microscopy 1
79
The Single-Sideband Edge Enhancement
Microscope
179
183
183
184
190
Fluorescence Probes
192
Pitfalls and Cures in Fluorescence Microscopy
193
Web Resources
193
12.
Various Types of Microscopes and
Accessories
195
Confocal Microscopes
195
Laser Microbeam Microscope
198
Optical Tweezers
199
Laser Capture
Microdissection
199
Laser
Doppler
Microscope
200
Centrifuge Microscope
200
Х
-Ray Microscope
201
Infrared Microscope
202
Nuclear Magnetic Resonance Imaging
Microscope
202
Stereo Microscopes
202
Scanning Probe Microscopes
203
Acoustic Microscope
204
Horizontal and Traveling Microscopes
205
Microscopes for Children
205
Microscope Accessories
205
Web Resources
206
13.
Video and Digital Microscopy
207
The Value of Video and Digital Microscopy
207
Video and Digital Cameras: The Optical to
Electrical Signal Converters
208
Monitors: Conversion of an Electronic Signal
14.
into an Optical Signal
216
Storage of Video and Digital Images
217
Connecting a Video System
218
Web Resources
218
Image Processing and Analysis
219
Analog Image Processing
219
Digital Image Processing
224
Enhancement Functions of Digital Image
Processors
225
Analysis Functions of Digital Image Processors
228
The Ethics of Digital Image Processing
229
Web Resources
230
176 15.
Laboratory Exercises
231
Laboratory
1 :
The Nature of Light and
Geometric Optics
231
Laboratory
2:
Physical Optics
233
Laboratory
3:
The Bright-Field Microscope
and Image Formation
234
Laboratory
4:
Phase-Contrast Microscopy,
Dark-Field Microscopy,
Rheinberg
Illumination, and Oblique Illumination
236
Laboratory
5:
Fluorescence Microscopy
239
Contents
ix
Laboratory
6:
Polarized Light
240
Laboratory
7:
Polarizing Light Microscopy
241
Laboratory
8:
Interference Microscopy
241
Laboratory
9:
Differential Interference
Contrast Microscopy and Hoffman Modulation
Contrast Microscopy
242
Laboratory
10:
Video and Digital Microscopy
and Analog and Digital Image Processing
243
Commercial Sources for Laboratory Equipment
and Specimens
245
References
247
Appendix I. A Final Exam
273
Appendix II. A Microscopist's Model of
the Photon
277
Index
285
Visit our companion website for additional book content, including answers to the final exam in the book, all of the images
from the book, and additional color images HYPERLINK "http://www.elsevierdirect.com/companions/9780123742346"
www.elsevierdirect.com/companions/9780123742346 |
| any_adam_object | 1 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T22:25:33Z |
| indexdate | 2024-07-09T21:23:06Z |
| institution | BVB |
| isbn | 9780123742346 |
| language | English |
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| spelling | Wayne, Randy Verfasser aut Light and video microscopy Randy Wayne Amsterdam [u.a.] Elsevier Acad. Press 2009 XII, 289 S., [2] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Lumière Microscopie Vidéomicroscopie Microscopy Video microscopy Videomikroskopie (DE-588)4367875-0 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Mikroskopie (DE-588)4039238-7 s DE-604 Videomikroskopie (DE-588)4367875-0 s Digitalisierung UB Bayreuth application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016802109&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wayne, Randy Light and video microscopy Lumière Microscopie Vidéomicroscopie Microscopy Video microscopy Videomikroskopie (DE-588)4367875-0 gnd Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4367875-0 (DE-588)4039238-7 |
| title | Light and video microscopy |
| title_auth | Light and video microscopy |
| title_exact_search | Light and video microscopy |
| title_exact_search_txtP | Light and video microscopy |
| title_full | Light and video microscopy Randy Wayne |
| title_fullStr | Light and video microscopy Randy Wayne |
| title_full_unstemmed | Light and video microscopy Randy Wayne |
| title_short | Light and video microscopy |
| title_sort | light and video microscopy |
| topic | Lumière Microscopie Vidéomicroscopie Microscopy Video microscopy Videomikroskopie (DE-588)4367875-0 gnd Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Lumière Microscopie Vidéomicroscopie Microscopy Video microscopy Videomikroskopie Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016802109&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT waynerandy lightandvideomicroscopy |