Programmed cell death: B The biology and therapeutic implications of cell death
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2008
|
| Schriftenreihe: | Methods in enzymology
446 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLVIII, 450 S Ill., graph. Darst. |
| ISBN: | 9780123744647 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV035086168 | ||
| 003 | DE-604 | ||
| 005 | 20081104 | ||
| 007 | t | ||
| 008 | 081007s2008 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123744647 |9 978-0-12-374464-7 | ||
| 035 | |a (OCoLC)316143986 | ||
| 035 | |a (DE-599)BVBBV035086168 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91G |a DE-355 |a DE-20 |a DE-19 |a DE-12 | ||
| 245 | 1 | 0 | |a Programmed cell death |n B |p The biology and therapeutic implications of cell death |c ed. by Roya Khosravi-Far ... |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2008 | |
| 300 | |a XLVIII, 450 S |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 446 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Khosravi-Far, Roya |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV035086045 |g 2 |
| 830 | 0 | |a Methods in enzymology |v 446 |w (DE-604)BV000000938 |9 446 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016754347&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016754347 | ||
Datensatz im Suchindex
| _version_ | 1804138040903335936 |
|---|---|
| adam_text | Contents
Contributors xin
Preface
xxi
Volumes in Series
1.
Analysis of Autophagic and Necrotic Cell Death in Dictyostelium
1
Corinne
Giusti,
Artemis
Kosta,
David Lam,
Emilie
Tresse,
Marie-Françoise
Luciani, and Pierre Golstein
1.
Introduction
2
2.
Growing
Dictyostelium
Cells in Monolayer Cultures
З
3.
Inducing
Autophagic Vacuolar
Cell Death
4
4.
Further Exploration of Autophagic Cell Death
6
5.
Inducing Necrotic Cell Death
10
6.
Further Exploration of Necrotic Cell Death
10
References
13
2.
Methods and Protocols for Studying Cell Death in
Drosophila
Π
Donna
Dentón,
Kathryn Mills, and Sharad Kumar
1.
Introduction
18
2.
Cell Death Analysis During Development
22
3.
Biochemical Analysis
27
4.
Genetic Analysis
31
References
35
3.
In Vivo and In Vitro Methods for Studying Apoptotic Cell
Engulfment in
Drosophila
39
Elizabeth A.
Silva,
Jemima Burden, and Nathalie
С
Franc
1.
Introduction
40
2.
Culture Conditions
41
3.
Labeling of Apoptotic Cells
43
4.
Visualizing the
Macrophages
46
5.
Quantification and Statistics
50
v¡
Contents
6.
Engulfment
¡η
Cell Culture
52
Acknowledgments
57
References
57
4.
A Mouse Mammary Epithelial Cell Model to Identify Molecular
Mechanisms Regulating Breast Cancer Progression
61
Vassiliki Karantza-Wadsworth and Eileen White
1.
Introduction
62
2.
A Novel Mouse Mammary Epithelial Cell Model
63
3.
Protocols
65
4.
Concluding Remarks
74
References
74
5.
Immortalized Mouse Epithelial Cell Models to Study the
Role of Apoptosis in Cancer
77
Robin Mathew, Kurt Degenhardt,
Liti
Haramaty,
Cristina
M.
Karp,
and Eileen White
1.
Introduction
78
2.
Immortalization of Baby Mouse Kidney, Mammary, Prostate,
and Ovarian Surface Epithelial Cells
81
3.
Development of
ІВМК
Cell Lines for Identifying the Role of
Apoptosis in Cancer
94
4.
Aposcreen: An iBMK Cell-Based Screen for the Identification of
Apoptosis-lnducing Compounds as Potential Anti-Cancer Agents
95
5.
Adaptation of the iBMK Cell Model System for the Evaluation
of Tissue-Specific Tumor-Promoting Functions
97
6.
Tumorigenicity Assay
100
7.
Noninvasive In Vivo Monitoring of Tumor Growth by Expression
of Fluorescence and Chemiluminescence Markers
102
8.
Tumor-Derived Cell Lines (jDCLs) as a Model System
for Epithelial Tumor-Promoting Functions
103
9.
Concluding Remarks and Future Perspectives
104
Acknowledgments
105
References
105
6. DNA
Damage Response and Apoptosis
107
Dragos
Plesca,
Suparna
Mazumder, and
Alexandru
Almasan
1.
Introduction
108
2.
Methods
110
Acknowledgments
120
References
120
Contents
vii
7. Phorbol Ester-Induced Apoptosis and
Sénescence
in
Cancer
Cell
Models 123
Liqing
Xiao,
M.
Cecilia Caino,
Vivian
A.
von Burstin,
Jose
L.
Oliva, and Marcelo
G. Kazanietz
1.
Introduction
124
2.
PKG5 as a Pro-Apoptotic Kinase
124
3.
PKC<5 as a
Mediator of Apoptotic Responses in
Prostate
Cancer
Cells:
The Emergence
of an
Autocrine
Paradigm
125
4.
Roles of PKCa and PKCe in LNCaP Cell Death and Survival
127
5.
Regulation of Cell Cycle and Senescence by PKC
128
6.
Determination of Phorbol Ester-Induced Apoptosis in
Prostate Cancer Cells
129
7.
Adenoviral Expression of PKC Isozymes
131
8.
RNA
Interference of PKC Isozymes
132
9.
Collection and Storage of CM from LNCaP Cells
133
10.
Determination of Senescence in
Respose
to Phorbol Esters
134
References
136
8.
Manipulation of PKC Isozymes by
RNA
Interference and Inducible
Expression of PKC Constructs
141
Alakananda
Basu,
Shalini D.
Persaud,
and Usha Sivaprasad
1.
Introduction
141
2.
Methods
143
Acknowledgments
155
References
155
9.
Studying pSB-Dependent Cell Death In Vitro and In Vivo
159
Kageaki Kuribayashi,
Niklas
Finnberg,
and Wafik S. El-Deiry
1.
Introduction
159
2.
Studying
рбЗ-Оерепаепі
Cell Death In Vitro
160
3.
Overexpression of p53 by Adenovirus (Ad)
160
4.
Silencing the p53 Gene by Retroviral Vector
164
5.
Studying the Apoptotic Response
165
6.
Studying
pöS-Dependent
Apoptosis In Vivo
165
References
172
10.
NF-kB as a Determinant of Distinct Cell Death Pathways
175
Irene L. Ch en, Stephen M. Hedrick, and Alexander Hoffmann
1.
Introduction
176
2.
Methods
178
v¡¡¡
Contents
3. DNA 180
4. Proteins 183
5.
Metabolic
Health 184
6.
Senescence Detection
185
References
186
11.
Purification and Bioassay of Hedgehog Ligands for the
Study of Cell Death and Survival
189
Pilar
Martinez-Chinchilla and Natalia A. Riobo
1.
Introduction
190
2.
Hedgehog and the Regulation of Cell Death
193
3.
Generation of
Recombinant
Hedgehog Ligands
195
References
201
12.
Experimental Approaches to Investigate the Proteasomal
Degradation Pathways Involved in Regulation of Apoptosis
205
Alan Tseng, Hiroyuki Inuzuka, Darning
Gao,
Amrik Singh, and Wenyi Wei
1.
Introduction
206
2.
General Instructions
207
3.
Protocols
207
References
222
13.
Ubiquitination Mediated by Inhibitor of Apoptosis Proteins
225
Sun-Mi Park, Shimin Hu, Tae H. Lee, and Xiaolu Yang
1.
Introduction
225
2.
In Vitro Ubiquitination Assay
226
3.
In Vivo Ubiquitination Assay
232
Acknowledgments
234
References
234
14.
Phosphatases and Regulation of Cell Death
237
Chi-Wu Chiang, Ling Yan, and Elizabeth Yang
1.
Introduction
238
2.
Identification of the Putative Phosphatase in Cells
240
3.
In Vitro Phosphatase Assays
248
4.
Interaction of the Phosphatase and Apoptotic Protein
249
5.
Linking Phosphatase Activity to Cell Death Protein Function
253
6.
Conclusion
255
Acknowledgment
255
References
255
Contents
¡χ
15.
Analysis of
Neuronal
Cell Death in Mammals
259
Marcello D Amelio,
Virve Cavallucci,
Adamo
Diamantini,
and
Francesco Cecconi
1.
Introduction
260
2.
Apoptosis Detection In Vitro
260
3.
Assessment of Cell Death
262
4.
Apoptosis Detection in Brain Development
269
5.
Detection of Synaptic Apoptosis (Synaptosis)
272
6.
Conclusions
275
Acknowledgments
275
References
275
16.
Dissecting Apoptosis and Intrinsic Death Pathways in the Heart
277
Danielle Weidman, James Shaw, Joseph Bednarczyk, Kelly M.
Regula,
Natalia Yurkova,
Tong
Zhang, Floribeth Aguilar, and
Lorrie A. Kirshenbaum
1.
Introduction
278
2.
Protocols
279
Acknowledgments
283
References
284
17.
Quantification of Vascular Endothelial Cell Apoptosis In Vivo
287
Xuefeng Zhang and Sareh
Parângi
1.
Introduction
287
2.
Protocols
288
References
292
18.
Recombinant
TRAIL and TRAIL Receptor Analysis
293
Nicholas Harper and Marion MacFarlane
1.
Introduction
294
2.
Generation of
Recombinant
TRAIL
295
3.
Analysis of TRAIL Receptors
302
4.
Concluding Remarks
311
Acknowledgments
311
References
312
Contents
19.
Analysis of
Tnf-Related
Apoptosis-lnducing Ligand In Vivo
Through Bone Marrow Transduction and Transplantation
315
Keli Song,
Raj Mariappan, and
Roya Khosravi-Far
1.
Introduction
316
2.
Generation of Retroviral Infection Medium
317
3.
Bone Marrow Cell Preparation
318
4.
Retroviral Transduction of Bone Marrow Cells
319
5.
Bone Marrow Transplantation
321
6.
Assessment of Bone Marrow Transplantation
322
7.
Pathologic Evaluation of TRAIL-Transduced Mice
323
8.
Methods for Immunohistochemical Staining for TRAIL Expression
326
9.
Applications that Use the Bone Marrow Transplantation Approach
328
Acknowledgments
329
References
329
20.
Overcoming Resistance to TRAIL-lnduced Apoptosis in
Prostate Cancer by Regulation of c-FLIP
333
Xiaoping Zhang, Wenhua Li, Aria F. Olumi
1.
Introduction
334
2.
Structure and Molecular Mechanisms of c-FLIP in Apoptosis
334
3.
Expression of c-FLIP
335
4.
Regulation of c-FLIP Expression
337
5.
Targeting c-FLIP to Enhance Apoptosis in Cancer Cells
341
6.
Methods and Materials
342
Acknowledgments
346
References
346
21.
Caspase Assays: Identifying Caspase Activity and
Substrates In Vitro and In Vivo
351
Cristina
Pop, Guy S. Salvesen, and Fiona L. Scott
1.
Introduction
352
2.
Caspase Methods
353
3.
Conclusion
365
References
366
22.
Synthesis and Biophysical Characterization of Stabilized
α
-Helices of BCL-2 Domains
369
Gregory H. Bird,
Federico
Bernal,
Kenneth
Pitter,
and
Loren
D.
Walensky
1.
Introduction
370
2.
Synthesis of Non-Natural
Amino
Acids for
Peptide
Stapling
372
Contents
x¡
3. Design
and Synthesis of Stabilized a-Helices of BCL-2 Domains
(SAHBs)
373
4.
Structural Assessment of SAHBs by Circular Dichroism (CD)
376
5.
Protease Resistance Testing of SAHBs
378
6.
Cell Permeability Screening of SAHBs
381
7.
Summary
383
Acknowledgments
384
References
384
23.
Dissection of the BCL-2 Family Signaling Network with
Stabilized a-Helices of BCL-2 Domains
387
Kenneth
Pitter,
Federico
Bernal,
James LaBelle, and
Loren
D.
Walensky
1.
Introduction
388
2.
BCL-2 Family Binding Measurements by Fluorescence
Polarization Assay
388
3.
In Vitro Release Assays as a Measure of Pro-Apoptotic Activity
392
4.
Measurement of Cellular Apoptosis Induction
400
5.
Identification of In Situ Mechanistic Targets of SAHBs
403
6.
Summary
405
Acknowledgments
405
References
405
24.
ERM-Mediated Genetic Screens in Mammalian Cells
409
Dan Liu and Zhou
Songyang
1.
introduction
409
2.
Methods and Design
411
3.
ERM-Mediated Genetic Screens and Re-PCA
415
4.
Conclusions
417
Acknowledgment
418
References
418
Author Index
421
Subject Index
445
|
| adam_txt |
Contents
Contributors xin
Preface
xxi
Volumes in Series
1.
Analysis of Autophagic and Necrotic Cell Death in Dictyostelium
1
Corinne
Giusti,
Artemis
Kosta,
David Lam,
Emilie
Tresse,
Marie-Françoise
Luciani, and Pierre Golstein
1.
Introduction
2
2.
Growing
Dictyostelium
Cells in Monolayer Cultures
З
3.
Inducing
Autophagic Vacuolar
Cell Death
4
4.
Further Exploration of Autophagic Cell Death
6
5.
Inducing Necrotic Cell Death
10
6.
Further Exploration of Necrotic Cell Death
10
References
13
2.
Methods and Protocols for Studying Cell Death in
Drosophila
Π
Donna
Dentón,
Kathryn Mills, and Sharad Kumar
1.
Introduction
18
2.
Cell Death Analysis During Development
22
3.
Biochemical Analysis
27
4.
Genetic Analysis
31
References
35
3.
In Vivo and In Vitro Methods for Studying Apoptotic Cell
Engulfment in
Drosophila
39
Elizabeth A.
Silva,
Jemima Burden, and Nathalie
С
Franc
1.
Introduction
40
2.
Culture Conditions
41
3.
Labeling of Apoptotic Cells
43
4.
Visualizing the
Macrophages
46
5.
Quantification and Statistics
50
v¡
Contents
6.
Engulfment
¡η
Cell Culture
52
Acknowledgments
57
References
57
4.
A Mouse Mammary Epithelial Cell Model to Identify Molecular
Mechanisms Regulating Breast Cancer Progression
61
Vassiliki Karantza-Wadsworth and Eileen White
1.
Introduction
62
2.
A Novel Mouse Mammary Epithelial Cell Model
63
3.
Protocols
65
4.
Concluding Remarks
74
References
74
5.
Immortalized Mouse Epithelial Cell Models to Study the
Role of Apoptosis in Cancer
77
Robin Mathew, Kurt Degenhardt,
Liti
Haramaty,
Cristina
M.
Karp,
and Eileen White
1.
Introduction
78
2.
Immortalization of Baby Mouse Kidney, Mammary, Prostate,
and Ovarian Surface Epithelial Cells
81
3.
Development of
ІВМК
Cell Lines for Identifying the Role of
Apoptosis in Cancer
94
4.
Aposcreen: An iBMK Cell-Based Screen for the Identification of
Apoptosis-lnducing Compounds as Potential Anti-Cancer Agents
95
5.
Adaptation of the iBMK Cell Model System for the Evaluation
of Tissue-Specific Tumor-Promoting Functions
97
6.
Tumorigenicity Assay
100
7.
Noninvasive In Vivo Monitoring of Tumor Growth by Expression
of Fluorescence and Chemiluminescence Markers
102
8.
Tumor-Derived Cell Lines (jDCLs) as a Model System
for Epithelial Tumor-Promoting Functions
103
9.
Concluding Remarks and Future Perspectives
104
Acknowledgments
105
References
105
6. DNA
Damage Response and Apoptosis
107
Dragos
Plesca,
Suparna
Mazumder, and
Alexandru
Almasan
1.
Introduction
108
2.
Methods
110
Acknowledgments
120
References
120
Contents
vii
7. Phorbol Ester-Induced Apoptosis and
Sénescence
in
Cancer
Cell
Models 123
Liqing
Xiao,
M.
Cecilia Caino,
Vivian
A.
von Burstin,
Jose
L.
Oliva, and Marcelo
G. Kazanietz
1.
Introduction
124
2.
PKG5 as a Pro-Apoptotic Kinase
124
3.
PKC<5 as a
Mediator of Apoptotic Responses in
Prostate
Cancer
Cells:
The Emergence
of an
Autocrine
Paradigm
125
4.
Roles of PKCa and PKCe in LNCaP Cell Death and Survival
127
5.
Regulation of Cell Cycle and Senescence by PKC
128
6.
Determination of Phorbol Ester-Induced Apoptosis in
Prostate Cancer Cells
129
7.
Adenoviral Expression of PKC Isozymes
131
8.
RNA
Interference of PKC Isozymes
132
9.
Collection and Storage of CM from LNCaP Cells
133
10.
Determination of Senescence in
Respose
to Phorbol Esters
134
References
136
8.
Manipulation of PKC Isozymes by
RNA
Interference and Inducible
Expression of PKC Constructs
141
Alakananda
Basu,
Shalini D.
Persaud,
and Usha Sivaprasad
1.
Introduction
141
2.
Methods
143
Acknowledgments
155
References
155
9.
Studying pSB-Dependent Cell Death In Vitro and In Vivo
159
Kageaki Kuribayashi,
Niklas
Finnberg,
and Wafik S. El-Deiry
1.
Introduction
159
2.
Studying
рбЗ-Оерепаепі
Cell Death In Vitro
160
3.
Overexpression of p53 by Adenovirus (Ad)
160
4.
Silencing the p53 Gene by Retroviral Vector
164
5.
Studying the Apoptotic Response
165
6.
Studying
pöS-Dependent
Apoptosis In Vivo
165
References
172
10.
NF-kB as a Determinant of Distinct Cell Death Pathways
175
Irene L. Ch'en, Stephen M. Hedrick, and Alexander Hoffmann
1.
Introduction
176
2.
Methods
178
v¡¡¡
Contents
3. DNA 180
4. Proteins 183
5.
Metabolic
Health 184
6.
Senescence Detection
185
References
186
11.
Purification and Bioassay of Hedgehog Ligands for the
Study of Cell Death and Survival
189
Pilar
Martinez-Chinchilla and Natalia A. Riobo
1.
Introduction
190
2.
Hedgehog and the Regulation of Cell Death
193
3.
Generation of
Recombinant
Hedgehog Ligands
195
References
201
12.
Experimental Approaches to Investigate the Proteasomal
Degradation Pathways Involved in Regulation of Apoptosis
205
Alan Tseng, Hiroyuki Inuzuka, Darning
Gao,
Amrik Singh, and Wenyi Wei
1.
Introduction
206
2.
General Instructions
207
3.
Protocols
207
References
222
13.
Ubiquitination Mediated by Inhibitor of Apoptosis Proteins
225
Sun-Mi Park, Shimin Hu, Tae H. Lee, and Xiaolu Yang
1.
Introduction
225
2.
In Vitro Ubiquitination Assay
226
3.
In Vivo Ubiquitination Assay
232
Acknowledgments
234
References
234
14.
Phosphatases and Regulation of Cell Death
237
Chi-Wu Chiang, Ling Yan, and Elizabeth Yang
1.
Introduction
238
2.
Identification of the Putative Phosphatase in Cells
240
3.
In Vitro Phosphatase Assays
248
4.
Interaction of the Phosphatase and Apoptotic Protein
249
5.
Linking Phosphatase Activity to Cell Death Protein Function
253
6.
Conclusion
255
Acknowledgment
255
References
255
Contents
¡χ
15.
Analysis of
Neuronal
Cell Death in Mammals
259
Marcello D'Amelio,
Virve Cavallucci,
Adamo
Diamantini,
and
Francesco Cecconi
1.
Introduction
260
2.
Apoptosis Detection In Vitro
260
3.
Assessment of Cell Death
262
4.
Apoptosis Detection in Brain Development
269
5.
Detection of Synaptic Apoptosis (Synaptosis)
272
6.
Conclusions
275
Acknowledgments
275
References
275
16.
Dissecting Apoptosis and Intrinsic Death Pathways in the Heart
277
Danielle Weidman, James Shaw, Joseph Bednarczyk, Kelly M.
Regula,
Natalia Yurkova,
Tong
Zhang, Floribeth Aguilar, and
Lorrie A. Kirshenbaum
1.
Introduction
278
2.
Protocols
279
Acknowledgments
283
References
284
17.
Quantification of Vascular Endothelial Cell Apoptosis In Vivo
287
Xuefeng Zhang and Sareh
Parângi
1.
Introduction
287
2.
Protocols
288
References
292
18.
Recombinant
TRAIL and TRAIL Receptor Analysis
293
Nicholas Harper and Marion MacFarlane
1.
Introduction
294
2.
Generation of
Recombinant
TRAIL
295
3.
Analysis of TRAIL Receptors
302
4.
Concluding Remarks
311
Acknowledgments
311
References
312
Contents
19.
Analysis of
Tnf-Related
Apoptosis-lnducing Ligand In Vivo
Through Bone Marrow Transduction and Transplantation
315
Keli Song,
Raj Mariappan, and
Roya Khosravi-Far
1.
Introduction
316
2.
Generation of Retroviral Infection Medium
317
3.
Bone Marrow Cell Preparation
318
4.
Retroviral Transduction of Bone Marrow Cells
319
5.
Bone Marrow Transplantation
321
6.
Assessment of Bone Marrow Transplantation
322
7.
Pathologic Evaluation of TRAIL-Transduced Mice
323
8.
Methods for Immunohistochemical Staining for TRAIL Expression
326
9.
Applications that Use the Bone Marrow Transplantation Approach
328
Acknowledgments
329
References
329
20.
Overcoming Resistance to TRAIL-lnduced Apoptosis in
Prostate Cancer by Regulation of c-FLIP
333
Xiaoping Zhang, Wenhua Li, Aria F. Olumi
1.
Introduction
334
2.
Structure and Molecular Mechanisms of c-FLIP in Apoptosis
334
3.
Expression of c-FLIP
335
4.
Regulation of c-FLIP Expression
337
5.
Targeting c-FLIP to Enhance Apoptosis in Cancer Cells
341
6.
Methods and Materials
342
Acknowledgments
346
References
346
21.
Caspase Assays: Identifying Caspase Activity and
Substrates In Vitro and In Vivo
351
Cristina
Pop, Guy S. Salvesen, and Fiona L. Scott
1.
Introduction
352
2.
Caspase Methods
353
3.
Conclusion
365
References
366
22.
Synthesis and Biophysical Characterization of Stabilized
α
-Helices of BCL-2 Domains
369
Gregory H. Bird,
Federico
Bernal,
Kenneth
Pitter,
and
Loren
D.
Walensky
1.
Introduction
370
2.
Synthesis of Non-Natural
Amino
Acids for
Peptide
Stapling
372
Contents
x¡
3. Design
and Synthesis of Stabilized a-Helices of BCL-2 Domains
(SAHBs)
373
4.
Structural Assessment of SAHBs by Circular Dichroism (CD)
376
5.
Protease Resistance Testing of SAHBs
378
6.
Cell Permeability Screening of SAHBs
381
7.
Summary
383
Acknowledgments
384
References
384
23.
Dissection of the BCL-2 Family Signaling Network with
Stabilized a-Helices of BCL-2 Domains
387
Kenneth
Pitter,
Federico
Bernal,
James LaBelle, and
Loren
D.
Walensky
1.
Introduction
388
2.
BCL-2 Family Binding Measurements by Fluorescence
Polarization Assay
388
3.
In Vitro Release Assays as a Measure of Pro-Apoptotic Activity
392
4.
Measurement of Cellular Apoptosis Induction
400
5.
Identification of In Situ Mechanistic Targets of SAHBs
403
6.
Summary
405
Acknowledgments
405
References
405
24.
ERM-Mediated Genetic Screens in Mammalian Cells
409
Dan Liu and Zhou
Songyang
1.
introduction
409
2.
Methods and Design
411
3.
ERM-Mediated Genetic Screens and Re-PCA
415
4.
Conclusions
417
Acknowledgment
418
References
418
Author Index
421
Subject Index
445 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV035086168 |
| ctrlnum | (OCoLC)316143986 (DE-599)BVBBV035086168 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01281nam a2200313 cc4500</leader><controlfield tag="001">BV035086168</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20081104 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">081007s2008 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123744647</subfield><subfield code="9">978-0-12-374464-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)316143986</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV035086168</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91G</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Programmed cell death</subfield><subfield code="n">B</subfield><subfield code="p">The biology and therapeutic implications of cell death</subfield><subfield code="c">ed. by Roya Khosravi-Far ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2008</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XLVIII, 450 S</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">446</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">...</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Khosravi-Far, Roya</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV035086045</subfield><subfield code="g">2</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in enzymology</subfield><subfield code="v">446</subfield><subfield code="w">(DE-604)BV000000938</subfield><subfield code="9">446</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016754347&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016754347</subfield></datafield></record></collection> |
| id | DE-604.BV035086168 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:08:59Z |
| indexdate | 2024-07-09T21:21:52Z |
| institution | BVB |
| isbn | 9780123744647 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016754347 |
| oclc_num | 316143986 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-12 |
| owner_facet | DE-91G DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-12 |
| physical | XLVIII, 450 S Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Programmed cell death B The biology and therapeutic implications of cell death ed. by Roya Khosravi-Far ... Amsterdam [u.a.] Elsevier, Acad. Press 2008 XLVIII, 450 S Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 446 Methods in enzymology ... Khosravi-Far, Roya Sonstige oth (DE-604)BV035086045 2 Methods in enzymology 446 (DE-604)BV000000938 446 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016754347&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Programmed cell death Methods in enzymology |
| title | Programmed cell death |
| title_auth | Programmed cell death |
| title_exact_search | Programmed cell death |
| title_exact_search_txtP | Programmed cell death |
| title_full | Programmed cell death B The biology and therapeutic implications of cell death ed. by Roya Khosravi-Far ... |
| title_fullStr | Programmed cell death B The biology and therapeutic implications of cell death ed. by Roya Khosravi-Far ... |
| title_full_unstemmed | Programmed cell death B The biology and therapeutic implications of cell death ed. by Roya Khosravi-Far ... |
| title_short | Programmed cell death |
| title_sort | programmed cell death the biology and therapeutic implications of cell death |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016754347&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035086045 (DE-604)BV000000938 |
| work_keys_str_mv | AT khosravifarroya programmedcelldeathb |