2D PAGE: sample preparation and fractionation 1
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in molecular biology
424 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 459 S. Ill., graph. Darst. |
| ISBN: | 9781588297228 9781603270649 |
Internformat
MARC
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| 245 | 1 | 0 | |a 2D PAGE |b sample preparation and fractionation |n 1 |c edited by Anton Posch |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2008 | |
| 300 | |a XIII, 459 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 424 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-016741564 | ||
Datensatz im Suchindex
| _version_ | 1804138022257557504 |
|---|---|
| adam_text | Contents
Preface
.................................................................
v
Contributors
............................................................
x¡
Part I: Sample Preparation Basics
1.
Mechanical/Physical Methods of Cell Disruption and Tissue
Homogenization
................................................ 3
Stanley Goldberg
2.
Bacteria and Yeast Cell Disruption Using Lytic Enzymes
............ 23
Oriana Salazar
3.
Sample Solublization Buffers for Two-Dimensional
Electrophoresis
.................................................. 35
Walter Weiss and
Angelika
Görg
4.
Quantitation of Protein in Samples Prepared for 2-D
Electrophoresis
.................................................. 43
Tom Berkelman
5.
Removal of Interfering Substances in Samples Prepared
for Two-Dimensional (2-D) Electrophoresis
...................... 51
Tom Berkelman
6.
Protein Concentration by Hydrophilic Interaction Chromatography
Combined with Solid Phase Extraction
........................... 63
Ulrich
Schneider
Part II: Protein Labeling Techniques
7.
Difference Gel Electrophoresis Based on Lys/Cys Tagging
........... 73
Reiner Westermeier
and Burghardt
Scheibe
8.
Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated
Acrylamide and 2-Vinylpyridine
................................. 87
Pier Giorgio
Righetti,
Roberto
Sebastiano,
and
Attilio Citterio
9.
Stable Isotope Labeling by
Amino
Acids in Cell Culture (SILAC)
.....101
Albrecht Gruhler
and
Irina Kratchmarova
10.
ICPL—Isotope-Coded Protein Label
................................ 113
Josef
Kellermann
11.
Radiolabeling for Two-Dimensional Gel Analysis
...................125
Hélian Boucherie, Aurélie Massoni,
and
Christelle
Monríbot-Espagne
vii
vj¡¡
Contents
Part III: Fractionation of Proteins by Chemical
Reagents and Chromatography
12.
Sequential Extraction of Proteins by Chemical Reagents
.............139
Stuart J. Cordwel!
13.
Reducing Sample Complexity by RP-HPLC: Beyond the Tip
of the Protein Expression Iceberg
................................147
Gert
Van den Bergh and Lutgarde Arckens
14.
Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange
Mini Spin Columns Before Two-Dimensional Gel
Electrophoresis
..................................................157
Ning
Liu and
Aran
Paulus
15.
Reducing the Complexity of the Escherichia
coli Proteome
by Chromatography on Reactive Dye Columns
..................167
Phillip Cash and Ian R. Booth
16.
Reducing Sample Complexity in Proteomics by Chromatofocusing
with Simple Buffer Mixtures
.....................................187
Hong Shen, Xiang Li, Charles J. Bieberich, and Douglas D.
Frey
17.
Fractionation of Proteins by Immobilized Metal Affinity
Chromatography
................................................205
Xuesong Sun, Jen-
Fu Chiu,
and Qing-Yu He
18.
Fractionation of Proteins by Heparin Chromatography
..............213
Sheng Xiong, Ling Zhang, and Qing-Yu He
Part IV: Fractionation of Proteins by Electrophoresis
Methods
19.
Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric
Focusing
........................................................225
Julie Hey, Anton
Posch,
Andrew Cohen,
Ning
Liu,
and
Adrianna Harbers
20.
Microscale
Isoelectric Focusing in Solution
.........................241
Мее
-Jung Han and David W.
Speicher
21.
Prefractionation, Enrichment, Desalting and Depleting
of Low Volume and Low Abundance Proteins and Peptides
Using the MF1
0.................................................257
Valerie Wasinger, Linda Ly, Anna Fitzgerald, and Brad Walsh
22.
Sample Prefractionation in Granulated Sephadex IEF Gels
..........277
Angelika Görg, Carsten Lück,
and Walter Weiss
23.
Free-Flow Electrophoresis System for Plasma Proteomic
Applications
....................................................287
Robert Wildgruber,
¡izu
Yi,
Mikkel Nissum,
Christoph Eckerskorn,
and Gerhard Weber
Contents ix
24. Protein Fractionation
by Preparative Electrophoresis
................301
Michael Fountoulakis and Ploumisti Dimitraki
Part
V: Enrichment Strategies for Organelles,
MULTIPROTEIN
COMPLEXES, AND SPECIFIC PROTEIN CLASSES
25.
Isolation of Endocitic Organelles by Density Gradient
Centrifugation
...................................................317
Mariana
Eça Guimarães de Araújo,
Lukas
Alfons
Huber,
and Taras Stasyk
26.
Isolation
of Highly Pure Rat Liver Mitochondria with the Aid of
Zone-Electrophoresis in a Free Flow Device (ZE-FFE)
.............333
Hans Zischka, Josef Lichtmannegger, Nora Jaegemann,
Luise
Jennen,
Daniela
Hamöller, Evamaria
Huber, Axel Walch,
Karl
H.
Summer, and
Martin
Göttlicher
27. Isolation
of
Proteins and Protein
Complexes
by Immunoprecipitation
.........................................349
Barbara Kaboord and Maria Perr
28. Isolation
of
Phosphoproteins.......................................365
Lawrence G.
Puente
and Lynn A. Megeney
29. Glycoprotein
Enrichment Through
Lectin
Affinity Techniques
.......373
Yehia Mechref, Milan
Madera,
and Milos V.
Novotný
30. Isolation
of Bacterial Cell Membranes Proteins Using Carbonate
Extraction
.......................................................397
Mark P. Molloy
31.
Enrichment of Membrane Proteins by Partitioning
in Detergent/Polymer Aqueous Two-Phase Systems
..............403
Henrik
Everberg,
Niklas
Gustavsson,
and
Folke Tjerneld
32.
The Isolation of Detergent-Resistant
Lipid
Rafts
for Two-Dimensional Electrophoresis
............................413
Ki
-Вит
Kim, Jae-Seon Lee, and Young-Gyu
Ko
33.
Isolation of Membrane Protein Complexes by Blue Native
Electrophoresis
..................................................423
Veronika
Reisinger and
Lutz
Α.
Eichacker
34.
Tissue
Microdissection
.............................................433
Heidi S. Erickson, John W. Gillespie,
and Michael R. Emmert-Buck
Index
...................................................................449
|
| adam_txt |
Contents
Preface
.
v
Contributors
.
x¡
Part I: Sample Preparation Basics
1.
Mechanical/Physical Methods of Cell Disruption and Tissue
Homogenization
. 3
Stanley Goldberg
2.
Bacteria and Yeast Cell Disruption Using Lytic Enzymes
. 23
Oriana Salazar
3.
Sample Solublization Buffers for Two-Dimensional
Electrophoresis
. 35
Walter Weiss and
Angelika
Görg
4.
Quantitation of Protein in Samples Prepared for 2-D
Electrophoresis
. 43
Tom Berkelman
5.
Removal of Interfering Substances in Samples Prepared
for Two-Dimensional (2-D) Electrophoresis
. 51
Tom Berkelman
6.
Protein Concentration by Hydrophilic Interaction Chromatography
Combined with Solid Phase Extraction
. 63
Ulrich
Schneider
Part II: Protein Labeling Techniques
7.
Difference Gel Electrophoresis Based on Lys/Cys Tagging
. 73
Reiner Westermeier
and Burghardt
Scheibe
8.
Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated
Acrylamide and 2-Vinylpyridine
. 87
Pier Giorgio
Righetti,
Roberto
Sebastiano,
and
Attilio Citterio
9.
Stable Isotope Labeling by
Amino
Acids in Cell Culture (SILAC)
.101
Albrecht Gruhler
and
Irina Kratchmarova
10.
ICPL—Isotope-Coded Protein Label
. 113
Josef
Kellermann
11.
Radiolabeling for Two-Dimensional Gel Analysis
.125
Hélian Boucherie, Aurélie Massoni,
and
Christelle
Monríbot-Espagne
vii
vj¡¡
Contents
Part III: Fractionation of Proteins by Chemical
Reagents and Chromatography
12.
Sequential Extraction of Proteins by Chemical Reagents
.139
Stuart J. Cordwel!
13.
Reducing Sample Complexity by RP-HPLC: Beyond the Tip
of the Protein Expression Iceberg
.147
Gert
Van den Bergh and Lutgarde Arckens
14.
Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange
Mini Spin Columns Before Two-Dimensional Gel
Electrophoresis
.157
Ning
Liu and
Aran
Paulus
15.
Reducing the Complexity of the Escherichia
coli Proteome
by Chromatography on Reactive Dye Columns
.167
Phillip Cash and Ian R. Booth
16.
Reducing Sample Complexity in Proteomics by Chromatofocusing
with Simple Buffer Mixtures
.187
Hong Shen, Xiang Li, Charles J. Bieberich, and Douglas D.
Frey
17.
Fractionation of Proteins by Immobilized Metal Affinity
Chromatography
.205
Xuesong Sun, Jen-
Fu Chiu,
and Qing-Yu He
18.
Fractionation of Proteins by Heparin Chromatography
.213
Sheng Xiong, Ling Zhang, and Qing-Yu He
Part IV: Fractionation of Proteins by Electrophoresis
Methods
19.
Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric
Focusing
.225
Julie Hey, Anton
Posch,
Andrew Cohen,
Ning
Liu,
and
Adrianna Harbers
20.
Microscale
Isoelectric Focusing in Solution
.241
Мее
-Jung Han and David W.
Speicher
21.
Prefractionation, Enrichment, Desalting and Depleting
of Low Volume and Low Abundance Proteins and Peptides
Using the MF1
0.257
Valerie Wasinger, Linda Ly, Anna Fitzgerald, and Brad Walsh
22.
Sample Prefractionation in Granulated Sephadex IEF Gels
.277
Angelika Görg, Carsten Lück,
and Walter Weiss
23.
Free-Flow Electrophoresis System for Plasma Proteomic
Applications
.287
Robert Wildgruber,
¡izu
Yi,
Mikkel Nissum,
Christoph Eckerskorn,
and Gerhard Weber
Contents ix
24. Protein Fractionation
by Preparative Electrophoresis
.301
Michael Fountoulakis and Ploumisti Dimitraki
Part
V: Enrichment Strategies for Organelles,
MULTIPROTEIN
COMPLEXES, AND SPECIFIC PROTEIN CLASSES
25.
Isolation of Endocitic Organelles by Density Gradient
Centrifugation
.317
Mariana
Eça Guimarães de Araújo,
Lukas
Alfons
Huber,
and Taras Stasyk
26.
Isolation
of Highly Pure Rat Liver Mitochondria with the Aid of
Zone-Electrophoresis in a Free Flow Device (ZE-FFE)
.333
Hans Zischka, Josef Lichtmannegger, Nora Jaegemann,
Luise
Jennen,
Daniela
Hamöller, Evamaria
Huber, Axel Walch,
Karl
H.
Summer, and
Martin
Göttlicher
27. Isolation
of
Proteins and Protein
Complexes
by Immunoprecipitation
.349
Barbara Kaboord and Maria Perr
28. Isolation
of
Phosphoproteins.365
Lawrence G.
Puente
and Lynn A. Megeney
29. Glycoprotein
Enrichment Through
Lectin
Affinity Techniques
.373
Yehia Mechref, Milan
Madera,
and Milos V.
Novotný
30. Isolation
of Bacterial Cell Membranes Proteins Using Carbonate
Extraction
.397
Mark P. Molloy
31.
Enrichment of Membrane Proteins by Partitioning
in Detergent/Polymer Aqueous Two-Phase Systems
.403
Henrik
Everberg,
Niklas
Gustavsson,
and
Folke Tjerneld
32.
The Isolation of Detergent-Resistant
Lipid
Rafts
for Two-Dimensional Electrophoresis
.413
Ki
-Вит
Kim, Jae-Seon Lee, and Young-Gyu
Ko
33.
Isolation of Membrane Protein Complexes by Blue Native
Electrophoresis
.423
Veronika
Reisinger and
Lutz
Α.
Eichacker
34.
Tissue
Microdissection
.433
Heidi S. Erickson, John W. Gillespie,
and Michael R. Emmert-Buck
Index
.449 |
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| id | DE-604.BV035073204 |
| illustrated | Illustrated |
| index_date | 2024-07-02T22:04:46Z |
| indexdate | 2024-07-09T21:21:35Z |
| institution | BVB |
| isbn | 9781588297228 9781603270649 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016741564 |
| oclc_num | 612053867 |
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| owner | DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-11 |
| physical | XIII, 459 S. Ill., graph. Darst. |
| publishDate | 2008 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | 2D PAGE sample preparation and fractionation 1 edited by Anton Posch Totowa, NJ Humana Press 2008 XIII, 459 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 424 Methods in molecular biology ... Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf Zweidimensionale Elektrophorese (DE-588)4221393-9 s DE-604 Posch, Anton Sonstige oth (DE-604)BV035073128 1 Methods in molecular biology 424 (DE-604)BV035362695 424 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016741564&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | 2D PAGE sample preparation and fractionation Methods in molecular biology Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| subject_GND | (DE-588)4221393-9 |
| title | 2D PAGE sample preparation and fractionation |
| title_auth | 2D PAGE sample preparation and fractionation |
| title_exact_search | 2D PAGE sample preparation and fractionation |
| title_exact_search_txtP | 2D PAGE sample preparation and fractionation |
| title_full | 2D PAGE sample preparation and fractionation 1 edited by Anton Posch |
| title_fullStr | 2D PAGE sample preparation and fractionation 1 edited by Anton Posch |
| title_full_unstemmed | 2D PAGE sample preparation and fractionation 1 edited by Anton Posch |
| title_short | 2D PAGE |
| title_sort | 2d page sample preparation and fractionation |
| title_sub | sample preparation and fractionation |
| topic | Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| topic_facet | Zweidimensionale Elektrophorese |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016741564&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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