Avian embryology:
Gespeichert in:
| Vorheriger Titel: | Methods in cell biology, erschienen als Methods in cell biology XXXX 51 |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2008
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in cell biology
87 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 1. Aufl. u.d.T.: Methods in avian embryology |
| Beschreibung: | XV, 409 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780125641746 |
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| 245 | 1 | 0 | |a Avian embryology |c ed. by Marianne Bronner-Fraser |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2008 | |
| 300 | |a XV, 409 S., [8] Bl. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in cell biology |v 87 | |
| 500 | |a 1. Aufl. u.d.T.: Methods in avian embryology | ||
| 650 | 7 | |a Poultry |2 cabt | |
| 650 | 7 | |a Chick Embryos |2 cabt | |
| 650 | 7 | |a Embryonic Development |2 cabt | |
| 650 | 7 | |a Surgical Operations |2 cabt | |
| 650 | 7 | |a Tissue Culture |2 cabt | |
| 650 | 7 | |a Hybridization |2 cabt | |
| 650 | 4 | |a Birds |x Embryology | |
| 650 | 4 | |a Birds |x Phylogeny | |
| 650 | 4 | |a Birds |x embryology | |
| 650 | 4 | |a Chick Embryo | |
| 650 | 4 | |a Embryology |x methods | |
| 650 | 4 | |a Vertebrates |x Evolution | |
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| 780 | 0 | 0 | |i 1. Auflage |a Methods in cell biology, erschienen als |t Methods in cell biology XXXX 51 |
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Datensatz im Suchindex
| _version_ | 1804137986702442496 |
|---|---|
| adam_text | CONTENTS
Contributors
Preface
PART I Ettibryological Microsurgery and Tissue
Culture Methods
1.
Operations on Primitive Streak Stage Avian Embryos
Andrea
Streit
and
Claudio
D.
Stern
I. Introduction
3
II. Staging of Embryos
4
III. Anatomy of the Primitive Streak Stage Embryo
6
IV. New Culture
8
V. Hensen s Node Grafts and Other Induction Assays
10
VI. Other Types of Graft
14
VII.
Fixing Operated Embryos for Analysis
15
References
16
2.
Quail—Chick Transplantations
Nicole he Dotiarin,
Françoise
Dietenen-
Lièvre,
Sophie
Creuzet,
and
Магіє
-Aimée
Teiltet
I. Introduction
20
II. Differential Diagnosis of Quail and Chick Cells
23
III. Material and Equipment
27
IV. Egg Holders
28
V. Preparation and Sealing of Eggs
31
VI. Neural Tissue Transplantations
31
VII.
Early Transplantations in Blastodiscs
39
VIII.
Transplantation of Epiblast or Primitive Streak Fragments
40
IX. Hemopoietic Organ Rudiment Transplantations
40
X. Genetic Manipulation by Electroporation in the Quail—Chick System
47
XI. Results, Discussion, and Perspectives
49
References
52
Contents
3.
Other Chimeras: Quail-Duck and Mouse-Chick
Peter Y. Lwigale and Richard A
.
Schneider
I. Introduction
60
II. Preparation of Quail, Duck, Chick, and Mouse Embryos
62
III. Generation and Analysis of Quail-Duck Chimeras
65
IV. Generation and Analysis of Mouse—Chick Chimeras
67
V. Conclusion
71
References
71
4.
Manipulations of Neural Crest Cells or Their Migratory Pathways
Marianne Bronner-Fraser and
Martín García-Castro
I. Introduction
76
II. Preparation of Avian Neural Crest Cultures
76
III. Induction and Specification Assays for Neural Crest Cells
84
IV. Microinjection of Cells and Antibodies into Embryos
88
V. Labeling of Neural Crest Cells In Vivo with Vital Dyes
90
VI. Grafting Techniques
92
VII.
Conclusions
94
References
95
5.
Embryo Slices and Strips: Guidance and Adhesion Assays in the
Avian Embryo
Catherine E. Krull and Kathryn Tosney
I. Introduction
98
II. Preparing and Culturing Embryo Slices
98
III. Preparing and Culturing Somite Strips
104
IV. Perspectives
108
References
112
6.
Neural Crest, Sensory Neuron, and Muscle Cultures
I Man M. Ln· and Peter V*. Lwigale
I. Introduction
116
II. Materials
117
III. Methods
1І9
IV. Neural Crest Culture
120
V. Sensory Neuron Culture
122
VI. Pectoral Muscle Culture
126
VII.
Results and Discussions
126
References
130
Contents
7.
Methods in
Avian
Embryológ)
Experimental
and Molecular Manipulation
of the Embryonic Chick Limb
Lee Niswander
I. Introduction
136
II. Visualization of the Limb Bud and Analysis of the
Experimental Outcome
137
III. Proximal—Distal Limb Development
139
IV. Anterior—Posterior Patterning
147
V. Dorsal-Ventral (D-V) Patterning
150
References
150
8.
Cell Division, Differentiation, and Death in Avian Embryos
Sara Ahlgmi
I. Introduction
153
II. Studies in Cell Death
154
III. Studies in Cell Proliferation and Differentiation
155
IV. Selected Protocols
156
References
164
PART II Labeling and
Transgenesis
Approaches
9.
In Sim Hybridization Analysis of Chick Embryos in Whole-Mount and
Tissue Sections
Herví Acloque,
David G.
Wilkinson, and
M.
Angela
Nieto
I. Introduction
170
II. Solutions
171
III. Single and Multiple Detection of
RNA
in Floating Sections
or Whole-Mount Embryos
172
IV. Photography and Sectioning
179
V. Whole-Mount Fluorescent In Situ Hybridization
179
VI. Photography and Sectioning
182
VII. In
Situ Hybridization to Tissue Sections
182
References
185
10.
Vital Labeling of Embryonic Cells Using Fluorescent Dyes and Proteins
Sujata Bhattaciiaryya
,
Paul M. Kidesa, and Scott E.
Fraser
I. Introduction
188
II. Iontophoretic
Microinjection
of Lineage Tracers
189
Contents
III. lontophoretic
Application
of
Dii
198
IV.
Relative
Advantages of Dextran and
Dii
200
V. Photoactivation
of Fluorescent Proteins in Single Cells
200
VI. Conclusions and Emerging Technologies
208
References
208
11.
Time-Lapse Imaging of the Early Avian Embryo
Max Ezin and Scott Fmser
I. Introduction
212
II. The Technology of Time-Lapse Imaging
214
III. Considerations Before Time-Lapse Imaging
218
IV. Methods
222
References
233
12.
Gain- and Loss-of-Function Approaches in the Chick Embryo
Tatjana
Sauka-Spetigler and Meyer Barembantn
I. Gain-of-Function by Electroporation
239
II. Loss-of-Function by Electroporation
243
III. Retrovirus-Mediated Protein Expression
251
References
254
13.
Manipulation and Electroporation of the Avian
Segmental
Plate
and Somites In Vitro
Tadahiro Hmum and
Olivier Pourquié
1.
Introduction
258
II. Rationale
259
III. Methods
260
IV. Materials
266
V. Discussion
267
VI. Summary
269
References
269
14.
Transposon-Mediated Stable Integration and Tetracycline-Inducible
Expression of Electroporated
Transgenes
in Chicken Embryos
Yoshtko Tdkahdihi, Tadayashi
Habilitée, Sliiniàii
Sakągawti,
Koichi Kawakami, and Yiiki Sato
I. Introduction
272
II. Tet-Inducibk· Expression of Electroporated
Transgenes
272
Contents
III.
Stable
Integration
of
Electroporated
Transgenes
276
IV.
Stage-Speciñc
Manipulation
of Stably Integrated
Transgenes
278
V. Summary
278
References
280
15.
Generating Transgenic Quail using Lentiviruses
Greg Poynter and Rusty Latisford
I. Introduction
282
II. Experimental Procedures
284
III. Concluding Remarks
292
References
292
PART III Functional Genomics
16.
Gene Discovery: Macroarrays and Microarrays
Laura S. Gammill and Vivian M. Lee
I. Introduction
298
II. Chicken as a Model System: Integrating Embryology and Genomics
298
III. The Array: Choosing a Platform
299
IV. Strategy: Devising the Best Screen Possible
302
V. Analysis: Sorting through Several Thousand Data Points
303
VI. Protocol: Macroarray Screening
304
References
311
17.
Dissection of Chick Genomic Regulatory Regions
Hisato Kondoh and
Masattori Uchikawa
I. Introduction
314
II. Rationale
315
III. Methods
319
IV. Discussion
329
References
335
18.
Computational Approaches to Finding and Analyzing rii-Regulatory Elements
C. Titus Brmm
I. Structure and Function of ns-Regulatory Elements
338
II. Effective ris-Regulatory Sequence Analysis
339
Contents
III. Approaches and Tools for Finding Conserved Sequence Elements
342
IV. Identifying Transcription Factor Binding Sites Computationally
352
References
363
19.
Investigating Regulatory Factors and Their
DNA
Binding Affinities Through
Real Time Quantitative PCR (RT-QPCR) and Chromatin Immunoprecipitation
(ChIP) Assays
Lisa A. Taneyhill and Meghan S. Adams
I. Introduction
368
II. Materials for RT-QPCR
373
III. General Principles and Definitions
375
IV. Types of Assays
376
V. Methods of Analysis
378
VI. Assay Setup
380
VII. ChIP
and RT-QPCR
381
VIII.
Conclusions and Perspectives
387
References
389
Index
391
Volumes in Series
401
|
| adam_txt |
CONTENTS
Contributors
Preface
PART I Ettibryological Microsurgery and Tissue
Culture Methods
1.
Operations on Primitive Streak Stage Avian Embryos
Andrea
Streit
and
Claudio
D.
Stern
I. Introduction
3
II. Staging of Embryos
4
III. Anatomy of the Primitive Streak Stage Embryo
6
IV. New Culture
8
V. Hensen's Node Grafts and Other Induction Assays
10
VI. Other Types of Graft
14
VII.
Fixing Operated Embryos for Analysis
15
References
16
2.
Quail—Chick Transplantations
Nicole he Dotiarin,
Françoise
Dietenen-
Lièvre,
Sophie
Creuzet,
and
Магіє
-Aimée
Teiltet
I. Introduction
20
II. Differential Diagnosis of Quail and Chick Cells
23
III. Material and Equipment
27
IV. Egg Holders
28
V. Preparation and Sealing of Eggs
31
VI. Neural Tissue Transplantations
31
VII.
Early Transplantations in Blastodiscs
39
VIII.
Transplantation of Epiblast or Primitive Streak Fragments
40
IX. Hemopoietic Organ Rudiment Transplantations
40
X. Genetic Manipulation by Electroporation in the Quail—Chick System
47
XI. Results, Discussion, and Perspectives
49
References
52
Contents
3.
Other Chimeras: Quail-Duck and Mouse-Chick
Peter Y. Lwigale and Richard A
.
Schneider
I. Introduction
60
II. Preparation of Quail, Duck, Chick, and Mouse Embryos
62
III. Generation and Analysis of Quail-Duck Chimeras
65
IV. Generation and Analysis of Mouse—Chick Chimeras
67
V. Conclusion
71
References
71
4.
Manipulations of Neural Crest Cells or Their Migratory Pathways
Marianne Bronner-Fraser and
Martín García-Castro
I. Introduction
76
II. Preparation of Avian Neural Crest Cultures
76
III. Induction and Specification Assays for Neural Crest Cells
84
IV. Microinjection of Cells and Antibodies into Embryos
88
V. Labeling of Neural Crest Cells In Vivo with Vital Dyes
90
VI. Grafting Techniques
92
VII.
Conclusions
94
References
95
5.
Embryo Slices and Strips: Guidance and Adhesion Assays in the
Avian Embryo
Catherine E. Krull and Kathryn Tosney
I. Introduction
98
II. Preparing and Culturing Embryo Slices
98
III. Preparing and Culturing Somite Strips
104
IV. Perspectives
108
References
112
6.
Neural Crest, Sensory Neuron, and Muscle Cultures
I "Man M. Ln· and Peter V*. Lwigale
I. Introduction
116
II. Materials
117
III. Methods
1І9
IV. Neural Crest Culture
120
V. Sensory Neuron Culture
122
VI. Pectoral Muscle Culture
126
VII.
Results and Discussions
126
References
130
Contents
7.
Methods in
Avian
Embryológ)'
Experimental
and Molecular Manipulation
of the Embryonic Chick Limb
Lee Niswander
I. Introduction
136
II. Visualization of the Limb Bud and Analysis of the
Experimental Outcome
137
III. Proximal—Distal Limb Development
139
IV. Anterior—Posterior Patterning
147
V. Dorsal-Ventral (D-V) Patterning
150
References
150
8.
Cell Division, Differentiation, and Death in Avian Embryos
Sara Ahlgmi
I. Introduction
153
II. Studies in Cell Death
154
III. Studies in Cell Proliferation and Differentiation
155
IV. Selected Protocols
156
References
164
PART II Labeling and
Transgenesis
Approaches
9.
In Sim Hybridization Analysis of Chick Embryos in Whole-Mount and
Tissue Sections
Herví' Acloque,
David G.
Wilkinson, and
M.
Angela
Nieto
I. Introduction
170
II. Solutions
171
III. Single and Multiple Detection of
RNA
in Floating Sections
or Whole-Mount Embryos
172
IV. Photography and Sectioning
179
V. Whole-Mount Fluorescent In Situ Hybridization
179
VI. Photography and Sectioning
182
VII. In
Situ Hybridization to Tissue Sections
182
References
185
10.
Vital Labeling of Embryonic Cells Using Fluorescent Dyes and Proteins
Sujata Bhattaciiaryya
,
Paul M. Kidesa, and Scott E.
Fraser
I. Introduction
188
II. Iontophoretic
Microinjection
of Lineage Tracers
189
Contents
III. lontophoretic
Application
of
Dii
198
IV.
Relative
Advantages of Dextran and
Dii
200
V. Photoactivation
of Fluorescent Proteins in Single Cells
200
VI. Conclusions and Emerging Technologies
208
References
208
11.
Time-Lapse Imaging of the Early Avian Embryo
Max Ezin and Scott Fmser
I. Introduction
212
II. The Technology of Time-Lapse Imaging
214
III. Considerations Before Time-Lapse Imaging
218
IV. Methods
222
References
233
12.
Gain- and Loss-of-Function Approaches in the Chick Embryo
Tatjana
Sauka-Spetigler and Meyer Barembantn
I. Gain-of-Function by Electroporation
239
II. Loss-of-Function by Electroporation
243
III. Retrovirus-Mediated Protein Expression
251
References
254
13.
Manipulation and Electroporation of the Avian
Segmental
Plate
and Somites In Vitro
Tadahiro Hmum and
Olivier Pourquié
1.
Introduction
258
II. Rationale
259
III. Methods
260
IV. Materials
266
V. Discussion
267
VI. Summary
269
References
269
14.
Transposon-Mediated Stable Integration and Tetracycline-Inducible
Expression of Electroporated
Transgenes
in Chicken Embryos
Yoshtko Tdkahdihi, Tadayashi
Habilitée, Sliiniàii
Sakągawti,
Koichi Kawakami, and Yiiki Sato
I. Introduction
272
II. Tet-Inducibk· Expression of Electroporated
Transgenes
272
Contents
III.
Stable
Integration
of
Electroporated
Transgenes
276
IV.
Stage-Speciñc
Manipulation
of Stably Integrated
Transgenes
278
V. Summary
278
References
280
15.
Generating Transgenic Quail using Lentiviruses
Greg Poynter and Rusty Latisford
I. Introduction
282
II. Experimental Procedures
284
III. Concluding Remarks
292
References
292
PART III Functional Genomics
16.
Gene Discovery: Macroarrays and Microarrays
Laura S. Gammill and Vivian M. Lee
I. Introduction
298
II. Chicken as a Model System: Integrating Embryology and Genomics
298
III. The Array: Choosing a Platform
299
IV. Strategy: Devising the Best Screen Possible
302
V. Analysis: Sorting through Several Thousand Data Points
303
VI. Protocol: Macroarray Screening
304
References
311
17.
Dissection of Chick Genomic Regulatory Regions
Hisato Kondoh and
Masattori Uchikawa
I. Introduction
314
II. Rationale
315
III. Methods
319
IV. Discussion
329
References
335
18.
Computational Approaches to Finding and Analyzing rii-Regulatory Elements
C. Titus Brmm
I. Structure and Function of ns-Regulatory Elements
338
II. Effective ris-Regulatory Sequence Analysis
339
Contents
III. Approaches and Tools for Finding Conserved Sequence Elements
342
IV. Identifying Transcription Factor Binding Sites Computationally
352
References
363
19.
Investigating Regulatory Factors and Their
DNA
Binding Affinities Through
Real Time Quantitative PCR (RT-QPCR) and Chromatin Immunoprecipitation
(ChIP) Assays
Lisa A. Taneyhill and Meghan S. Adams
I. Introduction
368
II. Materials for RT-QPCR
373
III. General Principles and Definitions
375
IV. Types of Assays
376
V. Methods of Analysis
378
VI. Assay Setup
380
VII. ChIP
and RT-QPCR
381
VIII.
Conclusions and Perspectives
387
References
389
Index
391
Volumes in Series
401 |
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| dewey-ones | 571 - Physiology & related subjects |
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| edition | 2. ed. |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV035048431 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:55:39Z |
| indexdate | 2024-07-09T21:21:01Z |
| institution | BVB |
| isbn | 9780125641746 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016717148 |
| oclc_num | 243819190 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-20 DE-188 |
| physical | XV, 409 S., [8] Bl. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Avian embryology ed. by Marianne Bronner-Fraser 2. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2008 XV, 409 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 87 1. Aufl. u.d.T.: Methods in avian embryology Poultry cabt Chick Embryos cabt Embryonic Development cabt Surgical Operations cabt Tissue Culture cabt Hybridization cabt Birds Embryology Birds Phylogeny Birds embryology Chick Embryo Embryology methods Vertebrates Evolution Embryologie (DE-588)4014562-1 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Vögel (DE-588)4063673-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Vögel (DE-588)4063673-2 s Embryologie (DE-588)4014562-1 s Methode (DE-588)4038971-6 s DE-604 Bronner-Fraser, Marianne Sonstige oth 1. Auflage Methods in cell biology, erschienen als Methods in cell biology XXXX 51 Methods in cell biology 87 (DE-604)BV002534878 87 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016717148&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Avian embryology Methods in cell biology Poultry cabt Chick Embryos cabt Embryonic Development cabt Surgical Operations cabt Tissue Culture cabt Hybridization cabt Birds Embryology Birds Phylogeny Birds embryology Chick Embryo Embryology methods Vertebrates Evolution Embryologie (DE-588)4014562-1 gnd Methode (DE-588)4038971-6 gnd Vögel (DE-588)4063673-2 gnd |
| subject_GND | (DE-588)4014562-1 (DE-588)4038971-6 (DE-588)4063673-2 (DE-588)4143413-4 |
| title | Avian embryology |
| title_auth | Avian embryology |
| title_exact_search | Avian embryology |
| title_exact_search_txtP | Avian embryology |
| title_full | Avian embryology ed. by Marianne Bronner-Fraser |
| title_fullStr | Avian embryology ed. by Marianne Bronner-Fraser |
| title_full_unstemmed | Avian embryology ed. by Marianne Bronner-Fraser |
| title_old | Methods in cell biology, erschienen als Methods in cell biology XXXX 51 |
| title_short | Avian embryology |
| title_sort | avian embryology |
| topic | Poultry cabt Chick Embryos cabt Embryonic Development cabt Surgical Operations cabt Tissue Culture cabt Hybridization cabt Birds Embryology Birds Phylogeny Birds embryology Chick Embryo Embryology methods Vertebrates Evolution Embryologie (DE-588)4014562-1 gnd Methode (DE-588)4038971-6 gnd Vögel (DE-588)4063673-2 gnd |
| topic_facet | Poultry Chick Embryos Embryonic Development Surgical Operations Tissue Culture Hybridization Birds Embryology Birds Phylogeny Birds embryology Chick Embryo Embryology methods Vertebrates Evolution Embryologie Methode Vögel Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016717148&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT bronnerfrasermarianne avianembryology |