Nitric oxide: G Oxidative and nitrosative stress in redox regulation of cell signaling
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
San Diego, Calif. [u.a.]
Acad. Press
2008
|
| Schriftenreihe: | Methods in enzymology
441 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLVIII, 436 S., [5] Bl. Ill., graph. Darst. |
| ISBN: | 9780123743091 |
Internformat
MARC
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| 003 | DE-604 | ||
| 005 | 20110803 | ||
| 007 | t | ||
| 008 | 080828s2008 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123743091 |9 978-0-12-374309-1 | ||
| 035 | |a (OCoLC)259975239 | ||
| 035 | |a (DE-599)BVBBV035027050 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91G |a DE-19 |a DE-355 |a DE-12 |a DE-20 | ||
| 245 | 1 | 0 | |a Nitric oxide |n G |p Oxidative and nitrosative stress in redox regulation of cell signaling |c ed. by Lester Packer |
| 264 | 1 | |a San Diego, Calif. [u.a.] |b Acad. Press |c 2008 | |
| 300 | |a XLVIII, 436 S., [5] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 441 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Packer, Lester |e Sonstige |4 oth | |
| 700 | 1 | |a Cadenas, Enrique |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV010923032 |g 7 |
| 830 | 0 | |a Methods in enzymology |v 441 |w (DE-604)BV000000938 |9 441 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016696087&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016696087 | ||
Datensatz im Suchindex
| _version_ | 1804137955723313152 |
|---|---|
| adam_text | Contents
Contributors
xiii
Volumes in Series
xxiii
1.
Protein 3-Nitrotyrosine in Complex Biological Samples:
Quantification by High-Pressure Liquid Chromatography/
Electrochemical Detection and Emergence of Proteomic
Approaches for Unbiased Identification of Modification Sites
1
Tal Nuriel, Ruba
S.
Deeb, David P. Hajjar, and Steven S. Gross
1.
Introduction
2
2.
Quantification of 3-NT in Proteins Using HPLC Separation and
Electrochemical (EC) Detection
3
3.
Protocol for Quantification of 3-NT in Hydrolyzed Proteins
Using HPLC-EC
3
4.
Beyond Quantification: Specification of 3-NT Sites in Proteins
7
Acknowledgment
16
References
16
2.
Selective Fluorogenic Derivatization of 3-Nitrotyrosine and
3,4-Dihydroxyphenylalanine in Peptides: A Method Designed
for Quantitative Proteomic Analysis
19
Victor S. Sharov, Elena S. Dremina, Justin
Pennington, Jacque
Killmer,
Christopher
Asmus,
Maria Thorson, Sung Jung Hong, Xiaobao Li,
John F. Stobaugh, and Christian
Schöneich
1.
Introduction
20
2.
Design of a Fluorogenic Derivatization Method
21
3.
Model Studies with 4-Methylcatechol and
2-Aminocresol
22
4.
Derivatization of a S-Nitrotyrosine-Containing Model
Peptide
with
Benzylamine and 4-Aminomethylbenzene Sulfonic Acid
23
5.
Conclusions
31
Acknowledgment
31
References
31
v¡
Contents
3.
Nitroalkenes:
Synthesis, Characterization, and Effects on
Macrophage
Activation
33
Ana
María
Ferreira,
Andrés
Trostchansky, Mariana Ferrari,
José M.
Souza,
and
Homero
Rubbo
1.
Introduction
34
2.
Synthesis and Characterization of Cholesteryl-Nitrolinoleate
36
3.
Synthesis and Identification of Nitroalkenes Isomers:
Mass Spectrometry Analysis of Nitroarachidonate
37
4.
Determination of
Lipid
Nitration during
Macrophage
Activation
39
5.
Effects of Nitroalkenes on NOS2 Induction by Activated
Macrophages
42
6.
Analysis of HO-1 Induction and NF-kB Activation
47
Acknowledgments
48
References
49
4.
In-Gel Detection of S-Nitrosated Proteins Using
Fluorescence Methods
53
Nicholas J. Kettenhofen, Xunde Wang, Mark T. Gladwin, and Neil Hogg
1.
Introduction
54
2.
Role of Trace Metal Ions in Ascorbate-Mediated Reduction of
S-Nitrosothiols
55
3.
Detection of Protein S-Nitrosation Using Fluorescent Labeling Methods
56
4.
Current Protocol for CyDye-Switch Method
59
5.
Difference Gel Electrophoresis Analysis of a Model Protein Mixture
61
6.
Sensitivity of Detection of the CyDye Label
62
7.
Fluorescence Detection of S-Nitrosated Proteins in Plasma
64
8.
Two-Dimensional Difference Gel Electrophoresis Detection of
S-Nitrosated Proteins
64
9.
Modifications of the Original Biotin-Switch Method
67
10.
Current Procedure for the Biotin-Switch Assay
68
11.
Conclusion
70
Acknowledgment
70
References
70
5.
The Arachidonate-Dependent Survival Signaling Preventing
Toxicity
in Monocytes/Macrophages Exposed to Peroxynitrite
73
Orazio
Cantoni,
Ilaria Tommasini,
and Liana Cerioni
1.
Introduction
74
2.
Materials and Methods
75
3.
Results and Discussion
tí
Acknowledgments
81
References
81
Contents
vii
6.
Practical
Approaches to Investigate
Redox
Regulation of Heat
Shock Protein Expression and Intracellular Glutathione
Redox
State
83
Vittorio Calabrese, Anna Signorile,
Carolin
Cornelius,
Cesare Mancuso,
Giovanni Scapagnini, Bernardo Ventimiglia,
Nicolo
Ragusa,
and
Albena Dinkova-Kostova
1.
Introduction
84
2.
Nitric Oxide and Cellular Stress Response: Role of Vitagenes
87
3.
Material and Methods
93
4.
Results
98
5.
Discussion
99
Acknowledgment
103
References
104
7.
Monitoring Oxidative Stress in Vascular Endothelial Cells in
Response to Fluid Shear Stress: From Biochemical Analyses to
Micro- and Nanotechnologies 111
Mahsa Rouhanizadeh, WakakoTakabe, LisongAi, Hongyu Yu, andTzung Hsiai
1.
Introduction
112
2.
In Vitro Monitoring of Reactive Species
116
3.
Measurement of Endothelial
ROS
Generation in Response
to Shear Stress
120
4.
Protein Oxidation and Nitration in Response to Shear Stress
125
5.
Emerging Technology: Micro- and Nanotechnology
133
6.
Summary
145
References
145
8.
Determination of 5-Nitrosothiols in Biological and Clinical
Samples Using Electron Paramagnetic Resonance Spectrometry
with Spin Trapping
151
Paul G. Winyard,
lona
A. Knight, Frances L. Shaw, Sophie A. Rocks,
Claire A. Davies, Paul Eggieton, Richard Haigh, Matthew Whiteman,
and Nigel Benjamin
1.
Introduction
152
2.
Materials and Methods
153
3.
Relative Yield of Spin-Trapped NO from S-Nitrosoalbumin in
Comparison with S-Nitrosoglutathione
156
4.
S-Nitrosoalbumin Recovery
157
5.
Potential Signal Contribution from W-Nitrosamines and Other
Possible Sources of NO
157
viii Contents
6.
Use of the EPR Assay for S-Nitrosothiols in Combination with
Isotopie
Labeling
158
Acknowledgments
159
References
159
9.
Novel Method for Measuring S-Nitrosothiols Using
Hydrogen
Sulfide 161
Xinjun
Teng,
T.
Scott Isbetl, Jack
H.
Crawford, Charles A. Bosworth,
Gregory I. Giles, Jeffrey R. Koenitzer, Jack R. Lancaster,
Jeannette
E.
Doeller, David W.
Kraus,
and Rakesh P.
Patel
1.
Introduction
162
2.
Experimental Manipulation of H2S
163
3.
Specificity for
^S-Dependent
Detection of RSNO
164
4.
Comparison of H2S vs Tri-iodide and Copper/Cysteine Based
Methods for RSNO Detection
166
5.
Effects of
pH
on HjS-Dependent RSNO Detection
167
6.
Effector Antifoam
169
7.
Summary and Conclusions
170
Acknowledgments
170
References
170
10.
Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins
173
Madia Trujillo,
Gerardo Ferrer-Sueta,
and Rafael
Radi
1.
Introduction
174
2.
General Considerations for Reagents Used
176
3.
Kinetics Studies of the Oxidative Part of the Catalytic Cycle
177
4.
Reductive Part of the Catalytic Cycle
190
5.
Conclusions
191
Acknowledgments
193
References
194
11.
Nitrocytochrome a Synthesis, Purification,
and Functional Studies
197
José M.
Souza,
Laura Castro,
Adriana
María
Cassina, Carlos
Batthyány,
and Rafael
Radi
1.
introduction
198
2.
Synthesis of Tyrosine-Nitrated Cytochrome
с
200
3.
Purification of the Mononitrated Cytochrome
с
Species
202
4.
Mapping the Sites of Tyrosine Nitration and Spectral Characterization
of the Nitrated Cytochrome
с
205
5.
Functional Consequences of Nitrated Cytochrome
с
208
Contents
¡χ
6. Concluding
Remarks
213
Acknowledgments
213
References
214
12.
Tyrosine Nitration, Dimerization, and Hydroxylation by
Peroxynitrite in Membranes as Studied by the
Hydrophobie
Probe
/V-ŕ-BOC-l-tyrosine ŕert-Butyl
Ester
217
Silvina Bartesaghi,
Gonzalo Peluffo,
Hao Zhang, Joy Joseph,
Balaraman
Kalyanaraman, and Rafael
Radi
1.
Introduction
218
2.
Methods
220
3.
Results
223
4.
Discussion
232
Acknowledgments
234
References
234
13.
Assessment of
Superoxide
Production and NADPH
Oxidase
Activity by HPLC Analysis of Dihydroethidium
Oxidation Products
237
Francisco R. M. Laurindo,
Denise
С.
Fernandes,
and
Celio
X.
С.
Santos
1.
Introduction
238
2.
Probes for Assessment of
Superoxide 239
3.
Dihydroethidium as
a
Superoxide
Probe
240
4.
HPLC Analysis of Dihydroethidium Oxidation in Cells and
Tissues: General Considerations
243
5.
Extraction of Fluorescent DHE-Derived Products in
Biological Samples
248
6.
Quantification of Fluorescent DHE-Derived Products
251
7.
Assessment of NADPH
Oxidase
Activity in Cell Membrane
Fraction by DHE Oxidation
254
8.
Summary and Conclusions
257
References
257
14.
Methods to Measure the Reactivity of Peroxynitrite-Derived
Oxidants Toward Reduced Fluoresceins and Rhodamines
261
Peter Wardman
1.
Introduction
262
2.
Reactants
262
3.
Methods
266
4.
Sources of Error and Scope for Improvements
in Methodology
278
x
Contents
5.
Conclusions
279
Acknowledgments
279
References
279
15.
Detection and Characterization of Peroxynitrite-lnduced
Modifications of Tyrosine, Tryptophan, and Methionine
Residues by Tandem Mass Spectrometry
283
Igor Rebrin, Catherine Bregere, Timothy K. Gallaher,
and Rajindar S. Sohal
1.
Introduction
284
2.
Experimental Procedures
285
3.
Anticipated Results
287
4.
Conclusion
292
Acknowledgment
292
References
293
16.
Reductive Gas-Phase Chemiluminescence and Flow Injection
Analysis for Measurement of the Nitric Oxide Pool in
Biological Matrices
295
Ulrike
Hendgen-Cotta,
Marijke
Grau,
Tienush Rassaf, Putrika Gharini,
Malte Keim,
and
Petra Kleinbongard
1.
Circulating and Resident Pool of Nitric Oxide
296
2.
Applied Methods for Determination of Nitrite, Nitrate, and
Nitroso
Species in Biological Matrices and Iron-Nitrosyls in Tissue
301
3.
Sample Processing
304
4.
Conclusions
309
Acknowledgments
309
References
309
17.
Detection and Measurement for the Modification
and Inactivation of Caspase by Nitrosative Stress In Vitro
and In Vivo
317
Hee-Jun Na, Hun-Taeg Chung, Kwon-Soo Ha, Hansoo Lee,
Young-Guen Kwon, Timothy R.
Billiar,
and Young-Myeong Kim
1.
Nitrosative Stress
318
2.
Biological Reactivity of Nitric Oxide
319
3.
S-Nitrosylating Chemistry
319
4.
S-Nitrosylating Specificity
320
5.
S-NitrosylationofCaspases
321
6.
Experimental Procedures
322
7.
Summary
325
Contents
x¡
Acknowledgment
325
References
325
18.
Interactive Relations between Nitric Oxide (NO) and Carbon
Monoxide (CO):
Heme
Oxygenase-l/CO Pathway Is a Key
Modulator in NO-Mediated Antiapoptosis and Anti-inflammation
329
Hun-Taeg Chung, Byung-Min Choi, Young-Guen Kwon, and Young-Myeong Kim
1.
Introduction
330
2.
Induction of HO-1 by the NOS/NO Pathway
331
3.
Antiapoptotic Roles of the NOS/HO-l Pathway
332
4.
Anti-inflammatory Roles of the NOS/HO-l Pathway
334
5.
Interactive Relations between NO and CO
335
6.
Conclusion
335
Acknowledgment
336
References
337
19.
Detection and Characterization of In Vivo Nitration and Oxidation
of Tryptophan Residues in Proteins
339
Catherine Bregere, Igor Rebrin, and Rajindar S. Sohal
1.
Introduction
340
2.
Methods
341
3.
Conclusions
346
Acknowledgment
348
References
348
20.
In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized
Rat Brain
351
Rui
M.
Barbosa,
Cátia
F.
Lourenço, Ricardo
M.
Santos,
Francois Pomerleau,
Peter Huettl, Greg
A.
Gerhardt, and
João Laranjinha
1.
Introduction
352
2.
Experimental
Considerations
353
3.
In Vivo Experiments: Results and Discussion
359
4.
Concluding Remarks
365
Acknowledgments
365
References
365
21.
Nitric Oxide and Cardiobiology-Methods for Intact Hearts
and Isolated Myocytes
369
Joshua M. Hare, Fariden
Beigi,
and
Konstantinos
Tziomalos
1.
Introduction
370
2.
Nitroso-Redox Signal Mediators
371
xii Contents
3.
Nitric
Oxide
and Signaling Through
Cysteine Thiols
372
4.
Nitroso-Redox Balance or Imbalance
373
5.
Nitric Oxide Synthases, NADPH
Oxidase,
and Xanthine
Oxidoreductase (XOR)
375
6.
Nitric
Oxide-Redox
Balance in Excitation-Contraction Coupling
377
7.
Techniques for Assessing Oxidative and Nitrosative Stress
377
8.
Techniques Used for Measuring S-Nitrosylation
382
9.
Activity Measurements
385
10.
Conclusions
386
Acknowledgments
387
References
387
22.
Microscopic Technique for the Detection of Nitric
Oxide-Dependent Angiogenesis in an Animal Model
393
Seung Namkoong, Byoung-Hee Chung, Kwon-Soo Ha, Hansoo Lee,
Young-Guen Kwon, and Young-Myeong Kim
1.
Introduction
394
2.
Experimental Procedures
395
3.
Results
397
4.
Summary and Conclusion
400
Acknowledgment
401
References
401
Author Index
403
Subject Index
429
|
| adam_txt |
Contents
Contributors
xiii
Volumes in Series
xxiii
1.
Protein 3-Nitrotyrosine in Complex Biological Samples:
Quantification by High-Pressure Liquid Chromatography/
Electrochemical Detection and Emergence of Proteomic
Approaches for Unbiased Identification of Modification Sites
1
Tal Nuriel, Ruba
S.
Deeb, David P. Hajjar, and Steven S. Gross
1.
Introduction
2
2.
Quantification of 3-NT in Proteins Using HPLC Separation and
Electrochemical (EC) Detection
3
3.
Protocol for Quantification of 3-NT in Hydrolyzed Proteins
Using HPLC-EC
3
4.
Beyond Quantification: Specification of 3-NT Sites in Proteins
7
Acknowledgment
16
References
16
2.
Selective Fluorogenic Derivatization of 3-Nitrotyrosine and
3,4-Dihydroxyphenylalanine in Peptides: A Method Designed
for Quantitative Proteomic Analysis
19
Victor S. Sharov, Elena S. Dremina, Justin
Pennington, Jacque
Killmer,
Christopher
Asmus,
Maria Thorson, Sung Jung Hong, Xiaobao Li,
John F. Stobaugh, and Christian
Schöneich
1.
Introduction
20
2.
Design of a Fluorogenic Derivatization Method
21
3.
Model Studies with 4-Methylcatechol and
2-Aminocresol
22
4.
Derivatization of a S-Nitrotyrosine-Containing Model
Peptide
with
Benzylamine and 4-Aminomethylbenzene Sulfonic Acid
23
5.
Conclusions
31
Acknowledgment
31
References
31
v¡
Contents
3.
Nitroalkenes:
Synthesis, Characterization, and Effects on
Macrophage
Activation
33
Ana
María
Ferreira,
Andrés
Trostchansky, Mariana Ferrari,
José M.
Souza,
and
Homero
Rubbo
1.
Introduction
34
2.
Synthesis and Characterization of Cholesteryl-Nitrolinoleate
36
3.
Synthesis and Identification of Nitroalkenes Isomers:
Mass Spectrometry Analysis of Nitroarachidonate
37
4.
Determination of
Lipid
Nitration during
Macrophage
Activation
39
5.
Effects of Nitroalkenes on NOS2 Induction by Activated
Macrophages
42
6.
Analysis of HO-1 Induction and NF-kB Activation
47
Acknowledgments
48
References
49
4.
In-Gel Detection of S-Nitrosated Proteins Using
Fluorescence Methods
53
Nicholas J. Kettenhofen, Xunde Wang, Mark T. Gladwin, and Neil Hogg
1.
Introduction
54
2.
Role of Trace Metal Ions in Ascorbate-Mediated Reduction of
S-Nitrosothiols
55
3.
Detection of Protein S-Nitrosation Using Fluorescent Labeling Methods
56
4.
Current Protocol for CyDye-Switch Method
59
5.
Difference Gel Electrophoresis Analysis of a Model Protein Mixture
61
6.
Sensitivity of Detection of the CyDye Label
62
7.
Fluorescence Detection of S-Nitrosated Proteins in Plasma
64
8.
Two-Dimensional Difference Gel Electrophoresis Detection of
S-Nitrosated Proteins
64
9.
Modifications of the Original Biotin-Switch Method
67
10.
Current Procedure for the Biotin-Switch Assay
68
11.
Conclusion
70
Acknowledgment
70
References
70
5.
The Arachidonate-Dependent Survival Signaling Preventing
Toxicity
in Monocytes/Macrophages Exposed to Peroxynitrite
73
Orazio
Cantoni,
Ilaria Tommasini,
and Liana Cerioni
1.
Introduction
74
2.
Materials and Methods
75
3.
Results and Discussion
tí
Acknowledgments
81
References
81
Contents
vii
6.
Practical
Approaches to Investigate
Redox
Regulation of Heat
Shock Protein Expression and Intracellular Glutathione
Redox
State
83
Vittorio Calabrese, Anna Signorile,
Carolin
Cornelius,
Cesare Mancuso,
Giovanni Scapagnini, Bernardo Ventimiglia,
Nicolo'
Ragusa,
and
Albena Dinkova-Kostova
1.
Introduction
84
2.
Nitric Oxide and Cellular Stress Response: Role of Vitagenes
87
3.
Material and Methods
93
4.
Results
98
5.
Discussion
99
Acknowledgment
103
References
104
7.
Monitoring Oxidative Stress in Vascular Endothelial Cells in
Response to Fluid Shear Stress: From Biochemical Analyses to
Micro- and Nanotechnologies 111
Mahsa Rouhanizadeh, WakakoTakabe, LisongAi, Hongyu Yu, andTzung Hsiai
1.
Introduction
112
2.
In Vitro Monitoring of Reactive Species
116
3.
Measurement of Endothelial
ROS
Generation in Response
to Shear Stress
120
4.
Protein Oxidation and Nitration in Response to Shear Stress
125
5.
Emerging Technology: Micro- and Nanotechnology
133
6.
Summary
145
References
145
8.
Determination of 5-Nitrosothiols in Biological and Clinical
Samples Using Electron Paramagnetic Resonance Spectrometry
with Spin Trapping
151
Paul G. Winyard,
lona
A. Knight, Frances L. Shaw, Sophie A. Rocks,
Claire A. Davies, Paul Eggieton, Richard Haigh, Matthew Whiteman,
and Nigel Benjamin
1.
Introduction
152
2.
Materials and Methods
153
3.
Relative Yield of Spin-Trapped NO' from S-Nitrosoalbumin in
Comparison with S-Nitrosoglutathione
156
4.
S-Nitrosoalbumin Recovery
157
5.
Potential Signal Contribution from W-Nitrosamines and Other
Possible Sources of NO"
157
viii Contents
6.
Use of the EPR Assay for S-Nitrosothiols in Combination with
Isotopie
Labeling
158
Acknowledgments
159
References
159
9.
Novel Method for Measuring S-Nitrosothiols Using
Hydrogen
Sulfide 161
Xinjun
Teng,
T.
Scott Isbetl, Jack
H.
Crawford, Charles A. Bosworth,
Gregory I. Giles, Jeffrey R. Koenitzer, Jack R. Lancaster,
Jeannette
E.
Doeller, David W.
Kraus,
and Rakesh P.
Patel
1.
Introduction
162
2.
Experimental Manipulation of H2S
163
3.
Specificity for
^S-Dependent
Detection of RSNO
164
4.
Comparison of H2S vs Tri-iodide and Copper/Cysteine Based
Methods for RSNO Detection
166
5.
Effects of
pH
on HjS-Dependent RSNO Detection
167
6.
Effector Antifoam
169
7.
Summary and Conclusions
170
Acknowledgments
170
References
170
10.
Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins
173
Madia Trujillo,
Gerardo Ferrer-Sueta,
and Rafael
Radi
1.
Introduction
174
2.
General Considerations for Reagents Used
176
3.
Kinetics Studies of the Oxidative Part of the Catalytic Cycle
177
4.
Reductive Part of the Catalytic Cycle
190
5.
Conclusions
191
Acknowledgments
193
References
194
11.
Nitrocytochrome a Synthesis, Purification,
and Functional Studies
197
José M.
Souza,
Laura Castro,
Adriana
María
Cassina, Carlos
Batthyány,
and Rafael
Radi
1.
introduction
198
2.
Synthesis of Tyrosine-Nitrated Cytochrome
с
200
3.
Purification of the Mononitrated Cytochrome
с
Species
202
4.
Mapping the Sites of Tyrosine Nitration and Spectral Characterization
of the Nitrated Cytochrome
с
205
5.
Functional Consequences of Nitrated Cytochrome
с
208
Contents
¡χ
6. Concluding
Remarks
213
Acknowledgments
213
References
214
12.
Tyrosine Nitration, Dimerization, and Hydroxylation by
Peroxynitrite in Membranes as Studied by the
Hydrophobie
Probe
/V-ŕ-BOC-l-tyrosine ŕert-Butyl
Ester
217
Silvina Bartesaghi,
Gonzalo Peluffo,
Hao Zhang, Joy Joseph,
Balaraman
Kalyanaraman, and Rafael
Radi
1.
Introduction
218
2.
Methods
220
3.
Results
223
4.
Discussion
232
Acknowledgments
234
References
234
13.
Assessment of
Superoxide
Production and NADPH
Oxidase
Activity by HPLC Analysis of Dihydroethidium
Oxidation Products
237
Francisco R. M. Laurindo,
Denise
С.
Fernandes,
and
Celio
X.
С.
Santos
1.
Introduction
238
2.
Probes for Assessment of
Superoxide 239
3.
Dihydroethidium as
a
Superoxide
Probe
240
4.
HPLC Analysis of Dihydroethidium Oxidation in Cells and
Tissues: General Considerations
243
5.
Extraction of Fluorescent DHE-Derived Products in
Biological Samples
248
6.
Quantification of Fluorescent DHE-Derived Products
251
7.
Assessment of NADPH
Oxidase
Activity in Cell Membrane
Fraction by DHE Oxidation
254
8.
Summary and Conclusions
257
References
257
14.
Methods to Measure the Reactivity of Peroxynitrite-Derived
Oxidants Toward Reduced Fluoresceins and Rhodamines
261
Peter Wardman
1.
Introduction
262
2.
Reactants
262
3.
Methods
266
4.
Sources of Error and Scope for Improvements
in Methodology
278
x
Contents
5.
Conclusions
279
Acknowledgments
279
References
279
15.
Detection and Characterization of Peroxynitrite-lnduced
Modifications of Tyrosine, Tryptophan, and Methionine
Residues by Tandem Mass Spectrometry
283
Igor Rebrin, Catherine Bregere, Timothy K. Gallaher,
and Rajindar S. Sohal
1.
Introduction
284
2.
Experimental Procedures
285
3.
Anticipated Results
287
4.
Conclusion
292
Acknowledgment
292
References
293
16.
Reductive Gas-Phase Chemiluminescence and Flow Injection
Analysis for Measurement of the Nitric Oxide Pool in
Biological Matrices
295
Ulrike
Hendgen-Cotta,
Marijke
Grau,
Tienush Rassaf, Putrika Gharini,
Malte Keim,
and
Petra Kleinbongard
1.
Circulating and Resident Pool of Nitric Oxide
296
2.
Applied Methods for Determination of Nitrite, Nitrate, and
Nitroso
Species in Biological Matrices and Iron-Nitrosyls in Tissue
301
3.
Sample Processing
304
4.
Conclusions
309
Acknowledgments
309
References
309
17.
Detection and Measurement for the Modification
and Inactivation of Caspase by Nitrosative Stress In Vitro
and In Vivo
317
Hee-Jun Na, Hun-Taeg Chung, Kwon-Soo Ha, Hansoo Lee,
Young-Guen Kwon, Timothy R.
Billiar,
and Young-Myeong Kim
1.
Nitrosative Stress
318
2.
Biological Reactivity of Nitric Oxide
319
3.
S-Nitrosylating Chemistry
319
4.
S-Nitrosylating Specificity
320
5.
S-NitrosylationofCaspases
321
6.
Experimental Procedures
322
7.
Summary
325
Contents
x¡
Acknowledgment
325
References
325
18.
Interactive Relations between Nitric Oxide (NO) and Carbon
Monoxide (CO):
Heme
Oxygenase-l/CO Pathway Is a Key
Modulator in NO-Mediated Antiapoptosis and Anti-inflammation
329
Hun-Taeg Chung, Byung-Min Choi, Young-Guen Kwon, and Young-Myeong Kim
1.
Introduction
330
2.
Induction of HO-1 by the NOS/NO Pathway
331
3.
Antiapoptotic Roles of the NOS/HO-l Pathway
332
4.
Anti-inflammatory Roles of the NOS/HO-l Pathway
334
5.
Interactive Relations between NO and CO
335
6.
Conclusion
335
Acknowledgment
336
References
337
19.
Detection and Characterization of In Vivo Nitration and Oxidation
of Tryptophan Residues in Proteins
339
Catherine Bregere, Igor Rebrin, and Rajindar S. Sohal
1.
Introduction
340
2.
Methods
341
3.
Conclusions
346
Acknowledgment
348
References
348
20.
In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized
Rat Brain
351
Rui
M.
Barbosa,
Cátia
F.
Lourenço, Ricardo
M.
Santos,
Francois Pomerleau,
Peter Huettl, Greg
A.
Gerhardt, and
João Laranjinha
1.
Introduction
352
2.
Experimental
Considerations
353
3.
In Vivo Experiments: Results and Discussion
359
4.
Concluding Remarks
365
Acknowledgments
365
References
365
21.
Nitric Oxide and Cardiobiology-Methods for Intact Hearts
and Isolated Myocytes
369
Joshua M. Hare, Fariden
Beigi,
and
Konstantinos
Tziomalos
1.
Introduction
370
2.
Nitroso-Redox Signal Mediators
371
xii Contents
3.
Nitric
Oxide
and Signaling Through
Cysteine Thiols
372
4.
Nitroso-Redox Balance or Imbalance
373
5.
Nitric Oxide Synthases, NADPH
Oxidase,
and Xanthine
Oxidoreductase (XOR)
375
6.
Nitric
Oxide-Redox
Balance in Excitation-Contraction Coupling
377
7.
Techniques for Assessing Oxidative and Nitrosative Stress
377
8.
Techniques Used for Measuring S-Nitrosylation
382
9.
Activity Measurements
385
10.
Conclusions
386
Acknowledgments
387
References
387
22.
Microscopic Technique for the Detection of Nitric
Oxide-Dependent Angiogenesis in an Animal Model
393
Seung Namkoong, Byoung-Hee Chung, Kwon-Soo Ha, Hansoo Lee,
Young-Guen Kwon, and Young-Myeong Kim
1.
Introduction
394
2.
Experimental Procedures
395
3.
Results
397
4.
Summary and Conclusion
400
Acknowledgment
401
References
401
Author Index
403
Subject Index
429 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T21:48:23Z |
| indexdate | 2024-07-09T21:20:31Z |
| institution | BVB |
| isbn | 9780123743091 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016696087 |
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| publishDate | 2008 |
| publishDateSearch | 2008 |
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| series | Methods in enzymology |
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| spelling | Nitric oxide G Oxidative and nitrosative stress in redox regulation of cell signaling ed. by Lester Packer San Diego, Calif. [u.a.] Acad. Press 2008 XLVIII, 436 S., [5] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 441 Methods in enzymology ... Packer, Lester Sonstige oth Cadenas, Enrique Sonstige oth (DE-604)BV010923032 7 Methods in enzymology 441 (DE-604)BV000000938 441 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016696087&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nitric oxide Methods in enzymology |
| title | Nitric oxide |
| title_auth | Nitric oxide |
| title_exact_search | Nitric oxide |
| title_exact_search_txtP | Nitric oxide |
| title_full | Nitric oxide G Oxidative and nitrosative stress in redox regulation of cell signaling ed. by Lester Packer |
| title_fullStr | Nitric oxide G Oxidative and nitrosative stress in redox regulation of cell signaling ed. by Lester Packer |
| title_full_unstemmed | Nitric oxide G Oxidative and nitrosative stress in redox regulation of cell signaling ed. by Lester Packer |
| title_short | Nitric oxide |
| title_sort | nitric oxide oxidative and nitrosative stress in redox regulation of cell signaling |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016696087&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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