Plant signal transduction: methods and protocols
This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction.
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2009
|
| Schriftenreihe: | Methods in molecular biology
479 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction. |
| Beschreibung: | XI, 357 S. Ill., graph. Darst. |
| ISBN: | 9781588299437 1588299430 |
Internformat
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| 245 | 1 | 0 | |a Plant signal transduction |b methods and protocols |c ed. by Thomas Pfannschmidt |
| 264 | 1 | |a New York, NY |b Humana Press |c 2009 | |
| 300 | |a XI, 357 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 479 | |
| 490 | 0 | |a Springer-Protocols | |
| 520 | 3 | |a This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction. | |
| 650 | 7 | |a Transduction du signal cellulaire chez les plantes |2 ram | |
| 650 | 2 | |a Transduction du signal | |
| 650 | 4 | |a Gene Expression Regulation, Plant | |
| 650 | 4 | |a Plant Physiological Phenomena | |
| 650 | 4 | |a Plant cellular signal transduction | |
| 650 | 4 | |a Plant molecular biology | |
| 650 | 4 | |a Plants |x growth & development | |
| 650 | 4 | |a Signal Transduction | |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
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| 830 | 0 | |a Methods in molecular biology |v 479 |w (DE-604)BV035362695 |9 479 | |
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Datensatz im Suchindex
| _version_ | 1804137928702558208 |
|---|---|
| adam_text | Contents
Preface
..........................................................
v
Contributors
......................................................
їх
1
Multiple Dimensions in Plant Signal Transduction: An Overview
.......... 1
]orge
ƒ.
Casal
2
Phenotyping of Arabidopsis Mutants for Developmental
Effects of Gene Deletions
....................................... 17
Cordelia
Bolle
3
Phenotyping of Abiotic Responses and Hormone Treatments
m
Arabidopsis
....................................................
35
Cordelia
Bolle
4
Accurate Real-time Reverse Transcription Quantitative PCR
.............. 61
Marco Klatte and
Petra
Bauer
5
Probing Spatio-temporal Intracellular Calcium Variations in Plants
......... 79
Axel Mith
ofer,
Christian Mazars, and
Massimo E.
Maffei
6
Dynamic
Redox
Measurements with
Redox-Sensitive
GFP in Plants by Confocal Laser Scanning Microscopy
................. 93
Andreas J. Meyer and
Thorsten Brach
7
Imaging of Reactive Oxygen Species in vivo
............................ 109
Steven
M. Driev
er, Michael f.
Fryer,
Philip
M. Mullineaux,
and Neil
R.
Baker
8
Identification of Thioredoxin Targeted Proteins
Using Thioredoxin Single
Cysteine
Mutant-immobilized Resin
........... 117
Ken Motohashi, Patrick G.
N.
Romano, and
Toru Hisabori
9
Determination of
m
vivo Protein Phosphorylation
in Photosynthetic Membranes
.................................... 133
Julia P. Vamonen, Alexander
V. Vener,
and Eva-Man
Aro
10
Examining Protein Stability and Its Relevance
for Plant Growth and Development
................................ 147
Claus Schwechheimer, Björn C.
Willige,
Melma Zourelidou,
and
Esther
M.
N. Dohmann
11
Probing Circadian Rhythms
in Chlamydomonas rhemhardtü
by Functional Proteomics
........................................ 173
Volker Wagner and Maria Mittag
12 Bimolecular
Fluorescence Complementation
(BiFC)
to Study Protein-protein Interactions in Living Plant Cells
......... 189
Katia
Schütze,
Klaus
Harter,
and Christina
Chah
an
13
Fluorescence Cross-Correlation Spectroscopy of Plant Proteins
........... 203
Hideki
Muto,
Masataka Ktnjo, and
Kotaro
T. Yamamoto
VII
viii Contents
14
The Determination of Protein-protein Interactions
by the Mating-based Split-ubiquitin-system (mbSUS)
.................. 217
Christopher Grefen,
Petr Obrdlik,
and
Klaus Harter
15
Functional Complementation of Yeast Mutants
to Study Plant Signalling Pathways
................................. 235
Norbert
Mehlmer,
Elisabeth Scheikl-Pourkhalil,
and
Markus
Teige
16
Phosphatase Activities Analyzed by
m
vivo Expressions
.................. 247
Alois Scbweijjhofer, Zahra Ayatollahi, and Irute Meskiene
17.
Chromatm Immunoprecipitation Experiments to Investigate
in vivo Binding of Arabidopsis Transcription
Factors to Target Sequences
...................................... 261
Ъепщтт
Fode and
Christiane Gatz
18
Fluorescence-based Electrophoretic Mobility Shift Assay
in the Analysis of DNA-binding Proteins
............................ 273
Sebastian Sterner and Thomas
Pfannschmidt
19
Analysis of Plant Regulatory
DNA
sequences
by the Yeast-One-Hybrid Assay
................................... 291
Dierk
Wanke
and
Klaus Harter
20
Analysis of Plant Regulatory
DNA
Sequences
by Transient Protoplast Assays and
Computer
Arded
Sequence Evaluation
.............................. 311
Kenneth W. Berendzen, Klaus
Harter,
and Dierk
Wanke
21
RNA-protein Interaction Mediating Post-transcnptional Regulation
in the Orcadian System
......................................... 337
Jan C. Schoning and
Dorothée Staiger
Index
............................................................... 353
|
| adam_txt |
Contents
Preface
.
v
Contributors
.
їх
1
Multiple Dimensions in Plant Signal Transduction: An Overview
. 1
]orge
ƒ.
Casal
2
Phenotyping of Arabidopsis Mutants for Developmental
Effects of Gene Deletions
. 17
Cordelia
Bolle
3
Phenotyping of Abiotic Responses and Hormone Treatments
m
Arabidopsis
.
35
Cordelia
Bolle
4
Accurate Real-time Reverse Transcription Quantitative PCR
. 61
Marco Klatte and
Petra
Bauer
5
Probing Spatio-temporal Intracellular Calcium Variations in Plants
. 79
Axel Mith
ofer,
Christian Mazars, and
Massimo E.
Maffei
6
Dynamic
Redox
Measurements with
Redox-Sensitive
GFP in Plants by Confocal Laser Scanning Microscopy
. 93
Andreas J. Meyer and
Thorsten Brach
7
Imaging of Reactive Oxygen Species in vivo
. 109
Steven
M. Driev
er, Michael f.
Fryer,
Philip
M. Mullineaux,
and Neil
R.
Baker
8
Identification of Thioredoxin Targeted Proteins
Using Thioredoxin Single
Cysteine
Mutant-immobilized Resin
. 117
Ken Motohashi, Patrick G.
N.
Romano, and
Toru Hisabori
9
Determination of
m
vivo Protein Phosphorylation
in Photosynthetic Membranes
. 133
Julia P. Vamonen, Alexander
V. Vener,
and Eva-Man
Aro
10
Examining Protein Stability and Its Relevance
for Plant Growth and Development
. 147
Claus Schwechheimer, Björn C.
Willige,
Melma Zourelidou,
and
Esther
M.
N. Dohmann
11
Probing Circadian Rhythms
in Chlamydomonas rhemhardtü
by Functional Proteomics
. 173
Volker Wagner and Maria Mittag
12 Bimolecular
Fluorescence Complementation
(BiFC)
to Study Protein-protein Interactions in Living Plant Cells
. 189
Katia
Schütze,
Klaus
Harter,
and Christina
Chah
an
13
Fluorescence Cross-Correlation Spectroscopy of Plant Proteins
. 203
Hideki
Muto,
Masataka Ktnjo, and
Kotaro
T. Yamamoto
VII
viii Contents
14
The Determination of Protein-protein Interactions
by the Mating-based Split-ubiquitin-system (mbSUS)
. 217
Christopher Grefen,
Petr Obrdlik,
and
Klaus Harter
15
Functional Complementation of Yeast Mutants
to Study Plant Signalling Pathways
. 235
Norbert
Mehlmer,
Elisabeth Scheikl-Pourkhalil,
and
Markus
Teige
16
Phosphatase Activities Analyzed by
m
vivo Expressions
. 247
Alois Scbweijjhofer, Zahra Ayatollahi, and Irute Meskiene
17.
Chromatm Immunoprecipitation Experiments to Investigate
in vivo Binding of Arabidopsis Transcription
Factors to Target Sequences
. 261
Ъепщтт
Fode and
Christiane Gatz
18
Fluorescence-based Electrophoretic Mobility Shift Assay
in the Analysis of DNA-binding Proteins
. 273
Sebastian Sterner and Thomas
Pfannschmidt
19
Analysis of Plant Regulatory
DNA
sequences
by the Yeast-One-Hybrid Assay
. 291
Dierk
Wanke
and
Klaus Harter
20
Analysis of Plant Regulatory
DNA
Sequences
by Transient Protoplast Assays and
Computer
Arded
Sequence Evaluation
. 311
Kenneth W. Berendzen, Klaus
Harter,
and Dierk
Wanke
21
RNA-protein Interaction Mediating Post-transcnptional Regulation
in the Orcadian System
. 337
Jan C. Schoning and
Dorothée Staiger
Index
. 353 |
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| isbn | 9781588299437 1588299430 |
| language | English |
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| series2 | Methods in molecular biology Springer-Protocols |
| spelling | Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt New York, NY Humana Press 2009 XI, 357 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 479 Springer-Protocols This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction. Transduction du signal cellulaire chez les plantes ram Transduction du signal Gene Expression Regulation, Plant Plant Physiological Phenomena Plant cellular signal transduction Plant molecular biology Plants growth & development Signal Transduction Labortechnik (DE-588)4123602-6 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Signaltransduktion (DE-588)4318717-1 s Labortechnik (DE-588)4123602-6 s b DE-604 Pfannschmidt, Thomas Sonstige oth Methods in molecular biology 479 (DE-604)BV035362695 479 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016678103&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plant signal transduction methods and protocols Methods in molecular biology Transduction du signal cellulaire chez les plantes ram Transduction du signal Gene Expression Regulation, Plant Plant Physiological Phenomena Plant cellular signal transduction Plant molecular biology Plants growth & development Signal Transduction Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Pflanzen (DE-588)4045539-7 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4318717-1 (DE-588)4045539-7 (DE-588)4143413-4 |
| title | Plant signal transduction methods and protocols |
| title_auth | Plant signal transduction methods and protocols |
| title_exact_search | Plant signal transduction methods and protocols |
| title_exact_search_txtP | Plant signal transduction methods and protocols |
| title_full | Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt |
| title_fullStr | Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt |
| title_full_unstemmed | Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt |
| title_short | Plant signal transduction |
| title_sort | plant signal transduction methods and protocols |
| title_sub | methods and protocols |
| topic | Transduction du signal cellulaire chez les plantes ram Transduction du signal Gene Expression Regulation, Plant Plant Physiological Phenomena Plant cellular signal transduction Plant molecular biology Plants growth & development Signal Transduction Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Pflanzen (DE-588)4045539-7 gnd |
| topic_facet | Transduction du signal cellulaire chez les plantes Transduction du signal Gene Expression Regulation, Plant Plant Physiological Phenomena Plant cellular signal transduction Plant molecular biology Plants growth & development Signal Transduction Labortechnik Signaltransduktion Pflanzen Aufsatzsammlung |
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