Evolution of flagellin perception in Arabidopsis thaliana and its relatives:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
2010
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 86, IX S. Ill., graph. Darst. |
| ISBN: | 9783869554549 |
Internformat
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| 245 | 1 | 0 | |a Evolution of flagellin perception in Arabidopsis thaliana and its relatives |c vorgelegt von Monika Madlen Vetter |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Göttingen |b Cuvillier |c 2010 | |
| 300 | |a XVI, 86, IX S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
CONTENTS
LIST OF FIGURES VIUE
LIST OF TABLES IX
ABBREVIATIONS X
SUMMARY XIUE
ZUSAMMENFASSUNG XV
1. INTRODUCTION 1
1.1. MOLECULAR BASIS OF PLANT-PATHOGEN INTERACTION 1
1.1.1. PAMP-TRIGGERED IMMUNITY 1
1.1.2. BACTERIAL COUNTERMEASURES AND EFFECTOR-TRIGGERED IMMUNITY 2
1.1.3. EXAMPLES OF R GENE EVOLUTION 3
1.2. RECOGNITION OF BACTERIAL PAMPS 4
1.2.1. BACTERIAL FLAGELLIN 4
1.2.2. FLS2-MEDIATED PERCEPTION OF FLAGELLIN 5
1.2.3. COMPARISON OF FLAGELLIN PERCEPTION IN PLANTS AND MAMMALS 5
1.2.4. EFR-MEDIATED PERCEPTION OF EF-TU 6
1.3. THE EVOLUTIONARY ECOLOGY OF IMMUNITY 6
1.3.1. SPECIFICITY IN HOST-PATHOGEN INTERACTIONS 7
1.3.2. COSTS OF IMMUNITY 7
1.3.3. IMPACT OF IMMUNE RESPONSES ON GROWTH AND DEVELOPMENT 7
1.3.4. ECOLOGICA] RELEVANCE OF ETI AND PTI 8
1.4. POPULATION GENETIC SIGNATURES OF SELECTION 8
1.4.1. FREQUENCY ANALYSIS OF ALLELIC VARIANCE 9
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1006222448
DIGITALISIERT DURCH
IMAGE 2
CONTENTS
1.4.2. MCDONALD-KREITMAN TESTS 9
1.4.3. LINKAGE DISEQUILIBRIUM AND HAPLOTYPE STRUCTURE 9
1.4.4. POPULATION DIFFERENTIATION 10
1.4.5. GENOME-WIDC ASSOCIATION MAPPING 10
1.5. AIM OF THE THESIS 11
2. MATERIALS AND METHODS 13
2.1. MATERIALS 13
2.1.1. PLANT MATERIALS 13
2.1.2. PATHOGEN (PSEUDOMONAS SYRINGAE PV. LOMATO DC3000) 13
2.1.3. SUPPLIERA AND MANUFACTUREN; 14
2.1.4. OLIGONUCLEOTIDES 14
2.1.5. ANTIBIOTICS 14
2.1.6. PEPTIDES 14
2.1.7. MEDIA 15
2.1.8. ANTIBODIES 16
2.1.9. BUFFERS AND SOLUTIONS 16
2.2. METHODS 16
2.2.1. MAINTENANCE AND CULTIVATION OF A. THALIANA 16
2.2.2. SEED SURFACE STERILIZATION 16
2.2.3. TRANSFORMATION OF AIFLS2 INTO A. THALIANA 17
2.2.4. MAINTENANCE OF PSEUDOMONAS SYRINGAE 17
2.2.5. PATHOGEN INFECTION ASSAY AND QUANTIFICATION 17
2.2.6. MOLECULAR BIOLOGICAL METHODS 18
2.2.7. BIOCHEMICAL METHODS 19
2.2.8. BIOASSAYS TO MONITOR PAMP RESPONSES 20
2.2.9. QUANTITATIVE TRAIT LOCUS ANALYSIS 21
2.2.10. DNA SEQUENCE ASSEMBLY AND ANALYSIS 22
2.2.11. CONTROL FOR POPULATION STRUCTURE 22
2.2.12. ANALYSIS OF POPULATION DIFFERENTIATION 22
3. RESULTS 25
3.1. SEQUENCE DIVERSITY AT THE FLS2 LOCUS 25
3.2. FUNCTIONAL AND PHENOTYPIC ANALYSIS OF FLS2 IN A WORLD WIDE SET OF
A. THALIANA . 30
3.2.1. RESPONSIVENESS TO VARIOUS FLG22 PEPTIDE VARIANTS 30
3.2.2. FUNCTIONAL ANALYSIS OFFLG22 PERCEPTION 34
IMAGE 3
CONTENTS
3.2.3. MOLECULAR CAUSES OF FLG22 BINDING VARIATION 36
3.2.4. NATURAL VARIATION IN PAMP-INDUCED PHENOTYPIC RESPONSES 43
3.2.5. CORRELATION OF FLG22BINDING AND FLOWERING TIME IN A. THALIANA 50
3.2.6. CORRELATION OF FLG22 BINDING AND BACTERIA GROWTH 52
3.2.7. IMPACT OF FLS2-MEDIATED IMMUNE RESPONSES ON REPRODUCTIVE FITNESS
. . 53
3.2.8. ALFLS2-MEDIATED INHIBITION OF SEEDLING GROWTH IN A. THALIANA 55
3.3. CHARACTERIZATION OF IMMUNITY IN LOCAL POPULATIONS OF A. THALIANA 57
3.3.1. SEQUENCE VARIATION AND GENETIC DIFFERENTIATION AT THE FLS2 LOCUS
IN LOCAL
POPULATIONS OF A. THALIANA 57
3.3.2. NATURAL VARIATION AND QUANTITATIVE DIFFERENTIATION OF IMMUNE
RESPONSES
IN LOCAL POPULATIONS OF A THALIANA 58
4. DISCUSSION 63
4.1. FLS2 SEQUENCE DIVERSITY 63
4.2. MOLECULAR CAUSES OF FLG22 BINDING VARIATION 64
4.3. CORRELATION OF FLG22 BINDING VARIATION AND FLG22-INDUCED IMMUNE
RESPONSES . . 65
4.4. GENETIC BASIS OF FLG22-INDUCED INHIBITION OF SEEDLING GROWTH 66
4.5. GENETIC DIFFERENTIATION OF FLS2 AND QUANTITATIVE DIFFERENTIATION OF
FLG22-INDUCED
IMMUNE RESPONSES 68
4.6. THE ECONOMIES OF IMMUNITY 69
5. CONCLUSION 73
REFERENCES 75
A. SUPPLEMENTARY TABLES I
A.L. TABLE OF A. THALIANA ACCESSIONS I
A.2. TABLE OF BRASSICACEAE SPECIES III
A.3. TABLE OF MUTANT AND TRANSGENIC A. THALIANA LINES IV
A.4. PHENOTYPIC CHARACTERIZATION OFFLS2 NON-FUNCTIONAL MUTANTS IV
A.5. LIST OF SUPPLIERS AND MANUFACTURERS V
ACKNOWLEDGMENTS VII
ERKLAERUNG IX
VII
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| discipline | Biologie |
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| spelling | Vetter, Monika Madlen Verfasser (DE-588)142368776 aut Evolution of flagellin perception in Arabidopsis thaliana and its relatives vorgelegt von Monika Madlen Vetter 1. Aufl. Göttingen Cuvillier 2010 XVI, 86, IX S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2010 Molekulare Evolution (DE-588)4812902-1 gnd rswk-swf Immunreaktion (DE-588)4133841-8 gnd rswk-swf Flagelline (DE-588)4344991-8 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Immunreaktion (DE-588)4133841-8 s Molekulare Evolution (DE-588)4812902-1 s Flagelline (DE-588)4344991-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022455005&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Vetter, Monika Madlen Evolution of flagellin perception in Arabidopsis thaliana and its relatives Molekulare Evolution (DE-588)4812902-1 gnd Immunreaktion (DE-588)4133841-8 gnd Flagelline (DE-588)4344991-8 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4812902-1 (DE-588)4133841-8 (DE-588)4344991-8 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | Evolution of flagellin perception in Arabidopsis thaliana and its relatives |
| title_auth | Evolution of flagellin perception in Arabidopsis thaliana and its relatives |
| title_exact_search | Evolution of flagellin perception in Arabidopsis thaliana and its relatives |
| title_full | Evolution of flagellin perception in Arabidopsis thaliana and its relatives vorgelegt von Monika Madlen Vetter |
| title_fullStr | Evolution of flagellin perception in Arabidopsis thaliana and its relatives vorgelegt von Monika Madlen Vetter |
| title_full_unstemmed | Evolution of flagellin perception in Arabidopsis thaliana and its relatives vorgelegt von Monika Madlen Vetter |
| title_short | Evolution of flagellin perception in Arabidopsis thaliana and its relatives |
| title_sort | evolution of flagellin perception in arabidopsis thaliana and its relatives |
| topic | Molekulare Evolution (DE-588)4812902-1 gnd Immunreaktion (DE-588)4133841-8 gnd Flagelline (DE-588)4344991-8 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Molekulare Evolution Immunreaktion Flagelline Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022455005&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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