Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Giessen
VVB Laufersweiler
2009
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Edition scientifique
|
| Schlagworte: | |
| Online-Zugang: | Abstract Inhaltsverzeichnis |
| Beschreibung: | VII, 96 S. Ill., graph. Darst. |
| ISBN: | 3835955527 |
Internformat
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| 650 | 7 | |a milk |2 cabt | |
| 650 | 7 | |a mycobacterium avium subsp. paratuberculosis |2 cabt | |
| 650 | 7 | |a bacterial diseases |2 cabt | |
| 650 | 7 | |a cattle |2 cabt | |
| 650 | 7 | |a dairy cattle |2 cabt | |
| 650 | 7 | |a cows |2 cabt | |
| 650 | 7 | |a polymerase chain reaction |2 cabt | |
| 650 | 7 | |a diagnosis |2 cabt | |
| 650 | 7 | |a faeces |2 cabt | |
| 650 | 7 | |a strains |2 cabt | |
| 650 | 7 | |a assays |2 cabt | |
| 650 | 7 | |a diagnostic techniques |2 cabt | |
| 650 | 7 | |a genetic diversity |2 cabt | |
| 650 | 7 | |a genetic variation |2 cabt | |
| 650 | 7 | |a disease prevalence |2 cabt | |
| 650 | 7 | |a human diseases |2 cabt | |
| 650 | 7 | |a man |2 cabt | |
| 650 | 7 | |a crohn's disease |2 cabt | |
| 650 | 7 | |a population genetics |2 cabt | |
| 650 | 7 | |a genotypes |2 cabt | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
DEDICATION I
DECLARATION II
LIST OF ABBREVIATIONS ILL
LIST OF TABLES VI
LIST OF FIGURES VII
1. INTRODUCTION 1
2. LITERATURE REVIEW: MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS .
2
2.1 TAXONOMY 2
2.2 NOMENCLATURE 2
2.3 CHARACTERISTIC FEATURES 3
2.4 JOHNE S DISEASE 4
2.4.1 INFECTION AND SUSCEPTIBILITY 4
2.4.2 GEOGRAPHICAL DISTRIBUTION AND SPREAD 4
2.4.3 TRANSMBSION 5
2.4.4 PALHOGENESIS: 5
2.4.5 CLINICAL PICTURE AND STAGES OF JOHNE S DISEASE 7
2.4.5.1 CLINICAL PICTURE 7
2.4.5.2 DISEASE STAGES 8
2.4.6 PATHOLOGY 8
2.4.7 MORBIDITY AND MORTALITY 9
2.4.8 ECONOMIC IMPORTANCE 9
2.4.9 DIAGNOSIS 10
2.4.9.1 DIRECT DETECTION OF THE CAUSAL AGENT 10
2.4.9.1.1 MICROSCOPIC EXAMINATION (ZIEHL-NEELSEN STAINING TECHNIQUE) 10
2.4.9.1.2 CULTURE METHODS 11
2.4.9.1.3 POLYMERASE CHAIN REACTION (PCR) 12
2.4.9.2 DETECTION OF HOST IMMUNOLOGICAL RESPONSE TO INFECTION -.. 13
2.4.9.2.1 ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELLSAS) 13
2.4.9.2.2 THE COMPLEMENT FIXATION TEST (CFT) 15
2.4.9.2.3 AGAR-GEL-IMMUNO-DIFFUSION TEST (AGID) 15
2.4.9.2.4 CELLULAR IMMUNITY TESTS 15
2.5 H U M AN RISK 16
2.6 DIVERSITY OF M. A VIUM SUBSPECIES PARATUBERCULOSIS STRAINS 17
3. MATERIAL AND METHODS 21
3.1 FIELD SAMPLES COLLECTION AND TRANSPORT 21
3.2 MICROSCOPIC INVESTIGATION OF FECES SAMPLES 21
3.3 DETECTION OF MAP BY CULTURE METHOD 23
3.3.1 DECONTAMINATION AND CULTURE OF FECES SAMPLES 23
3.3.2 DECONTAMINATION AND CULTURE OF MILK SAMPLES 23
3.4 ISOLATES IDENTIFICATION 23
3.4.1 PHENOTYPIC CHARACTERS AND COLONIES COUNT 23
3.4.2 ZIEHL-NEELSEN STAIN 24
3.4.3 MLDEPENDENCY. 24
3.4.4 MOLECULAR IDENTIFICATION 24
3.5 MOLECULAR DIAGNOSIS 24
3.5.1 SELECTION OF THE TARGET SEQUENCES 24
3.5.1.1 DETERMINATION OF DIFFERENT PCR PRIMERS SPECIFICITY 24
3.5.1.2 ASSESSMENT OF SYSTEMS SENSIUEVIEES 27
3.5.1.3 SPIKED SAMPLES. 28
3.5.2 EXTRACTION OF DNA FROM FECES AND MILK SAMPLES 28
3.5.3 SELECTION OF DIAGNOSTIC PCR ASSAYS 29
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1000205576
DIGITALISIERT DURCH
IMAGE 2
3.5.3.1 IS90I) NESTED PCR ASSAY 30
3.5.3.1.1 METHODOLOGIES APPLIED TO IMPROVE THE PERFORMANCE OF THE NESTED
PCR 30 3.5.3.2 F57 CONVENTIONAL PCR ASSAY 31
3.6 MOLECULAR CHARACTERIZATION OF ISOLATES (RESTRICTION FRAGMENT LENGTH
POLYMORPHISM) 31 3.6.1 M. AVIUM SUBSPECIES PARATUBERCULOSIS ISOLATES 31
3.6.1.1 MOLECULAR IDENTIFICATION OF ISOLATES 32
3.6.1.2 STRAINS GROWTH CONDITIONS 33
3.6.2 ISOLATION OFGENOMIC DNA 33
3.6.3 DIGESTION OFGENOMIC DNA WITH RESTRICTION ENDONUCLEASE BSTELL AND
SEPARATION OF DNA FRAGMENTS BY ELECTROPHORESIS 33
3.6.4 DOWNWARD SOUTHERN -BLOTTING 34
3.6.5 PREPARATION OF MAP IS900 DNA PROBE 35
3.6.6 HYBRIDIZATION WITH DIG - DNA PROBE AND MEMBRANE DETECTION 36
3.6.7 DETECTION AND VISUALIZATION 36
3.6.8 COMPUTER ASSISTED ANALYSIS OF RFLP FINGERPRINTS 37
3.6.9 NOMENCLATURE OF THE IS900 - BSTELL RFLP TYPES 37
3.6.10 SIMPSON S DIVERSITY INDEX CALCULATION 37
4. RESULTS 38
4.1 MOLECULAR DIAGNOSIS 38
4.1.1 SELECTION OF THE TARGET SEQUENCES 38
4.1.1.1 DETERMINATION OF DIFFERENT PCR PRIMERS SPECIFICITY 38
4.1.1.2 ASSESSMENT OF SYSTEMS SENSITIVITY 41
4.1.2 MAP DIAGNOSTIC PCR ASSAYS 43
4.1.2.1 1S900 NESTED PCR ASSAY 43
4.1.2.2 F57 CONVENTIONAL PCR ASSAY 44
4.2 DIRECT ZN STAINING OF FECES SAMPLES 45
4.3 DETECTION OF MAP BY CULTURE METHOD 46
4.3.1 ISOLATION OF MAP FROM FECES SAMPLES 46
4.3.2 ISOLATION OF MAP FROM MILK SAMPLES 46
4.3.3 COMPARISON OF ALL APPLIED TESTS FOR MAP ISOLATES IDENTIFICATION 47
4.4 COMPARATIVE ANALYSIS OF DIAGNOSTIC METHODS WITH RESPECT TO ANIMALS
SHEDDING STATUS .48 4.5 HERDS MONITORING 50
4.6 MOLECULAR CHARACTERIZATION OF ISOLATES 52
4.6.1 MOLECULAR IDENTIFICATION OF ISOLATES 52
4.6.2 DNA PROBE LABELING 52
4.6.3 IS9O0 BSTELL-RFLP 53
4.6.3.1 OUSTERS AND PROFILES 53
4.6.3.2 GEOGRAPHICAL DISTRIBUTION OFTHEIS900-BSTELL RFLP TYPES 57
4.6.3.3 COMPARATIVE ANALYSIS OF THE RFLP WITH VARIOUS TYPING SCHEMES
APPLIED IN DIFFERENT LABORATORIES 62 4.6.3.4 HOSTS - PATTERNS
RELATIONSHIP 63
5. DISCUSSION.........
..........................................................................................................
65
5.1 CONCLUSION AND CLINICAL RELEVANCE 73
5.2 EPIDEMIOLOGICAL RELEVANCE 74
6.
SUMMARY......................................................-..................-......--............*.--.....--...75
7.
ZUSANUIIEIIFASSUNG.............».».»...».»................»*».».».»....»».....»»»»»*.»??
8. REAGENTS, KITS, BUFFERS AND EQUIPMENTS 79
8.1 REAGENTS AND KITS 79
8.2 BUFFERS 81
8.3 LABORATORY EQUIPMENTS AND TUBES 82
8.4 IS900 R F LP PATTERNS COMPUTER ASCERTAINMENT 83
9. REFERENCES ~~~ 84
ACKNOWLEDGMENT 96
|
| any_adam_object | 1 |
| author | Salem, Mohamed |
| author_facet | Salem, Mohamed |
| author_role | aut |
| author_sort | Salem, Mohamed |
| author_variant | m s ms |
| building | Verbundindex |
| bvnumber | BV026885828 |
| ctrlnum | (OCoLC)540989916 (DE-599)DNB1000205576 |
| dewey-full | 636.20896340756 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.20896340756 |
| dewey-search | 636.20896340756 |
| dewey-sort | 3636.20896340756 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Salem, Mohamed Verfasser aut Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization submitted by Mohamed Salem 1. Aufl. Giessen VVB Laufersweiler 2009 VII, 96 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Giessen, Univ., Diss., 2009 milk cabt mycobacterium avium subsp. paratuberculosis cabt bacterial diseases cabt cattle cabt dairy cattle cabt cows cabt polymerase chain reaction cabt diagnosis cabt faeces cabt strains cabt assays cabt diagnostic techniques cabt genetic diversity cabt genetic variation cabt disease prevalence cabt human diseases cabt man cabt crohn's disease cabt population genetics cabt genotypes cabt Isolierung Mikrobiologie (DE-588)4193882-3 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Mycobacterium paratuberculosis (DE-588)4520169-9 gnd rswk-swf Milchkuh (DE-588)4169916-6 gnd rswk-swf Hessen (DE-588)4024729-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hessen (DE-588)4024729-6 g Milchkuh (DE-588)4169916-6 s Mycobacterium paratuberculosis (DE-588)4520169-9 s Isolierung Mikrobiologie (DE-588)4193882-3 s Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 http://library.vetmed.fu-berlin.de/ResourceList/details/207767 lizenzfrei Abstract DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022411642&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Salem, Mohamed Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization milk cabt mycobacterium avium subsp. paratuberculosis cabt bacterial diseases cabt cattle cabt dairy cattle cabt cows cabt polymerase chain reaction cabt diagnosis cabt faeces cabt strains cabt assays cabt diagnostic techniques cabt genetic diversity cabt genetic variation cabt disease prevalence cabt human diseases cabt man cabt crohn's disease cabt population genetics cabt genotypes cabt Isolierung Mikrobiologie (DE-588)4193882-3 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Mycobacterium paratuberculosis (DE-588)4520169-9 gnd Milchkuh (DE-588)4169916-6 gnd |
| subject_GND | (DE-588)4193882-3 (DE-588)4256726-9 (DE-588)4520169-9 (DE-588)4169916-6 (DE-588)4024729-6 (DE-588)4113937-9 |
| title | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization |
| title_auth | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization |
| title_exact_search | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization |
| title_full | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization submitted by Mohamed Salem |
| title_fullStr | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization submitted by Mohamed Salem |
| title_full_unstemmed | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization submitted by Mohamed Salem |
| title_short | Detection of Mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization |
| title_sort | detection of mycobacterium avium subspecies paratuberculosis in dairy herds and comparative molecular characterization |
| topic | milk cabt mycobacterium avium subsp. paratuberculosis cabt bacterial diseases cabt cattle cabt dairy cattle cabt cows cabt polymerase chain reaction cabt diagnosis cabt faeces cabt strains cabt assays cabt diagnostic techniques cabt genetic diversity cabt genetic variation cabt disease prevalence cabt human diseases cabt man cabt crohn's disease cabt population genetics cabt genotypes cabt Isolierung Mikrobiologie (DE-588)4193882-3 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Mycobacterium paratuberculosis (DE-588)4520169-9 gnd Milchkuh (DE-588)4169916-6 gnd |
| topic_facet | milk mycobacterium avium subsp. paratuberculosis bacterial diseases cattle dairy cattle cows polymerase chain reaction diagnosis faeces strains assays diagnostic techniques genetic diversity genetic variation disease prevalence human diseases man crohn's disease population genetics genotypes Isolierung Mikrobiologie Polymerase-Kettenreaktion Mycobacterium paratuberculosis Milchkuh Hessen Hochschulschrift |
| url | http://library.vetmed.fu-berlin.de/ResourceList/details/207767 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022411642&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT salemmohamed detectionofmycobacteriumaviumsubspeciesparatuberculosisindairyherdsandcomparativemolecularcharacterization |