Molecular characterization and probiotic potential of Kimere lactobacilli:
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1. Verfasser: | |
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
Göttingen
Cuvillier
2009
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Ausgabe: | 1. Aufl. |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | IX, 117 S. Ill., graph. Darst. |
ISBN: | 9783869550565 |
Internformat
MARC
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035 | |a (OCoLC)723955078 | ||
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245 | 1 | 0 | |a Molecular characterization and probiotic potential of Kimere lactobacilli |c vorgelegt von Patrisio Njiru Njeru |
250 | |a 1. Aufl. | ||
264 | 1 | |a Göttingen |b Cuvillier |c 2009 | |
300 | |a IX, 117 S. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
502 | |a Zugl.: Kiel, Univ., Diss., 2009 | ||
650 | 7 | |a probiotics |2 cabt | |
650 | 7 | |a lactobacillus |2 cabt | |
650 | 7 | |a Kenya |2 cabt | |
650 | 7 | |a genetics |2 cabt | |
650 | 7 | |a characterization |2 cabt | |
650 | 7 | |a Pennisetum glaucum |2 cabt | |
650 | 0 | 7 | |a Perlhirse |0 (DE-588)4209339-9 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Probiotikum |0 (DE-588)4338112-1 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Isolierung |g Mikrobiologie |0 (DE-588)4193882-3 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Lactobacillus |0 (DE-588)4166370-6 |2 gnd |9 rswk-swf |
655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
689 | 0 | 0 | |a Perlhirse |0 (DE-588)4209339-9 |D s |
689 | 0 | 1 | |a Lactobacillus |0 (DE-588)4166370-6 |D s |
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Datensatz im Suchindex
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adam_text | Titel: Molecular characterization and probiotic potential of Kimere lactobacilli
Autor: Njeru, Patrisio Njiru
Jahr: 2009
TABLE OF CONTENTS
TABLE OF CONTENTS
Dedication.....................................................................................................................................................ii
TABLE OF CONTENTS.............................................................................................................................iii
LIST OF ABBREVIATIONS......................................................................................................................vi
ABSTRACT...............................................................................................................................................viii
ZUSAMMENFASSUNG............................................................................................................................ix
CHAPTER 1.........................................................................................................................1
MOLECULAR METHODS IN PROBIOTIC RESEARCH........................................................................1
ABSTRACT..................................................................................................................................................1
1 INTRODUCTION............................................................................................................................1
2 GENETIC FINGER PRINTING METHODS..................................................................................3
3 POLYMERASE CHAIN REACTION (PCR)-BASED METHODS...............................................5
4 HYBRIDIZATION METHODS.......................................................................................................6
5 REFERENCES...............................................................................................................................11
Chapter II........................................................................................................................20
Microbiological quality and biodiversity of lactobacilli from Kimere, a spontaneously fermented pearl
millet dough from Mbeere, Kenya (East Africa)........................................................................................20
ABSTRACT................................................................................................................................................21
1 INTRODUCTION..........................................................................................................................22
1.1 Kimere Production.........................................................................................................................23
2.2 Microbiological Quality of Kimere................................................................................................26
2 MATERIALS AND METHODS....................................................................................................28
2.1 Kimere sampling and sample preparation......................................................................................28
2.2 pH determination in Kimere...........................................................................................................28
2.3 Microbiological analysis of Kimere...............................................................................................29
2.4 Isolation and biochemical characterization of lactobacilli.............................................................29
2.5 Molecular characterization of lactobacilli......................................................................................30
2.5.1 Amplified ribosomal DNA restriction analysis (ARDRA)..............................................................................30
2.5.2 Species-specific PCR for L. fermentum.........................................................................................................31
2.5.3 16SrDNA sequencing....................................................................................................................................31
2.5.4 Pulsed-Field Gel Electrophoresis (PFGE)....................................................................................................31
2.6 Data analysis..................................................................................................................................32
Thesis Njeru P.N 2009
3 RESULTS.......................................................................................................................................32
3.1 Microbiological quality of Kimere.................................................................................................32
3.2 Biochemical characterization of lactobacilli..................................................................................33
3.3 Species-Specific PCR identification..............................................................................................38
3.4 ARDRA of Kimere isolates and type strains..................................................................................38
3.5 Partial sequence of the 16S rDNA.................................................................................................40
3.6 PFGE profiles of Kimere isolates...................................................................................................42
4 DISCUSSION.................................................................................................................................43
5 ACKNOWLEDGEMENTS............................................................................................................47
6 REFERENCES...............................................................................................................................47
Chapter III......................................................................................................................53
Strain specific immunomodulation by Lactobacillus fermentum strains isolated from Kimere; an African
fermented pearl millet (Pennisetum glaucum) dough...............................................................................53
Abstract.......................................................................................................................................................54
1 INTRODUCTION..........................................................................................................................55
2 MATERIAL AND METHODS......................................................................................................58
2.1 Bacteria cultures.............................................................................................................................58
2.2 Resistance to bile salts...................................................................................................................59
2.3 Acid tolerance................................................................................................................................60
2.4 Testing immunomodulation...........................................................................................................60
2.4.1 PBMCs Isolation...........................................................................................................................................60
2.4.2 Co-incubation of PBMCs and bacteria.........................................................................................................61
2.4.3 Cytokines assay.............................................................................................................................................61
2.5 Statistics.........................................................................................................................................61
3 RESULTS.......................................................................................................................................62
3.1 Resistance to bile salts and low pH................................................................................................62
3.2 Cytokine production patterns.........................................................................................................64
4 DISCUSSIONS...............................................................................................................................67
5 ACKNOWLEDGEMENTS............................................................................................................70
6 REFERENCES...............................................................................................................................70
CHAPTER IV....................................................................................................................74
Suppression Subtractive Hybridization of Kimere Lactobaciilus strains....................................................74
Abstract............................................................................... 75
iv
TABLE OF CONTENTS
1 INTRODUCTION..........................................................................................................................76
2 MATERIALS AND METHODS....................................................................................................79
2.1 Bacterial strains..............................................................................................................................79
2.2 Isolation of genomic DNA.............................................................................................................79
2.3 Suppression subtractive hybridization (SSH)................................................................................80
2.4 Cloning into a TA vector................................................................................................................81
2.5 Plasmid DNA isolation and Recombinants screening....................................................................81
2.6 PCR screening of isolates...............................................................................................................81
2.7 Dot hybridization...........................................................................................................................82
2.8 DNA Sequencing and analysis.......................................................................................................82
3 RESULTS.......................................................................................................................................83
3.1 Construction of SSH library...........................................................................................................83
3.2 Sequence Protein homology and DNA matches............................................................................85
4 DISCUSSION.................................................................................................................................88
4.1 Relationship between Kl-Lbl SSH library and cell-surface structures.........................................89
4.1.1 Exopolysaccharides (EPS)............................................................................................................................89
4.1.2 Lipoteichoic and Wall teichoic acids.............................................................................................................90
4.1.3 Peptidogfycans..............................................................................................................................................90
4.2 Relationship between Kl-Lbl SSH library and Immune modulation...........................................91
5 ACKNOWLEDGEMENTS............................................................................................................93
6 REFERENCES...............................................................................................................................93
Chapter V......................................................................................................................101
1 GENERAL DISCUSSIONS AND APPLICATION OF RESULTS............................................101
1.1 Probiotics and Allergy..................................................................................................................103
1.2 Probiotics and Inflammatory Bowel Diseases (IBD)...................................................................104
1.3 Probiotics and Autoimmune Diseases..........................................................................................106
1.4 Superantigens and Thl/Th2 balance............................................................................................106
1.5 Conclusions and recommendations..............................................................................................107
2 REFERENCES.............................................................................................................................108
Acknowledgement....................................................................................................................................113
List of Tables............................................................................................................................................115
List of Figures...........................................................................................................................................116
|
any_adam_object | 1 |
author | Njeru, Patrisio Njiru 1975- |
author_GND | (DE-588)139457585 |
author_facet | Njeru, Patrisio Njiru 1975- |
author_role | aut |
author_sort | Njeru, Patrisio Njiru 1975- |
author_variant | p n n pn pnn |
building | Verbundindex |
bvnumber | BV026733202 |
ctrlnum | (OCoLC)723955078 (DE-599)DNB996162534 |
dewey-full | 615.329 |
dewey-hundreds | 600 - Technology (Applied sciences) |
dewey-ones | 615 - Pharmacology and therapeutics |
dewey-raw | 615.329 |
dewey-search | 615.329 |
dewey-sort | 3615.329 |
dewey-tens | 610 - Medicine and health |
discipline | Biologie Medizin |
edition | 1. Aufl. |
format | Thesis Book |
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spelling | Njeru, Patrisio Njiru 1975- Verfasser (DE-588)139457585 aut Molecular characterization and probiotic potential of Kimere lactobacilli vorgelegt von Patrisio Njiru Njeru 1. Aufl. Göttingen Cuvillier 2009 IX, 117 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Kiel, Univ., Diss., 2009 probiotics cabt lactobacillus cabt Kenya cabt genetics cabt characterization cabt Pennisetum glaucum cabt Perlhirse (DE-588)4209339-9 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Isolierung Mikrobiologie (DE-588)4193882-3 gnd rswk-swf Lactobacillus (DE-588)4166370-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Perlhirse (DE-588)4209339-9 s Lactobacillus (DE-588)4166370-6 s Probiotikum (DE-588)4338112-1 s Isolierung Mikrobiologie (DE-588)4193882-3 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022272852&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Njeru, Patrisio Njiru 1975- Molecular characterization and probiotic potential of Kimere lactobacilli probiotics cabt lactobacillus cabt Kenya cabt genetics cabt characterization cabt Pennisetum glaucum cabt Perlhirse (DE-588)4209339-9 gnd Probiotikum (DE-588)4338112-1 gnd Isolierung Mikrobiologie (DE-588)4193882-3 gnd Lactobacillus (DE-588)4166370-6 gnd |
subject_GND | (DE-588)4209339-9 (DE-588)4338112-1 (DE-588)4193882-3 (DE-588)4166370-6 (DE-588)4113937-9 |
title | Molecular characterization and probiotic potential of Kimere lactobacilli |
title_auth | Molecular characterization and probiotic potential of Kimere lactobacilli |
title_exact_search | Molecular characterization and probiotic potential of Kimere lactobacilli |
title_full | Molecular characterization and probiotic potential of Kimere lactobacilli vorgelegt von Patrisio Njiru Njeru |
title_fullStr | Molecular characterization and probiotic potential of Kimere lactobacilli vorgelegt von Patrisio Njiru Njeru |
title_full_unstemmed | Molecular characterization and probiotic potential of Kimere lactobacilli vorgelegt von Patrisio Njiru Njeru |
title_short | Molecular characterization and probiotic potential of Kimere lactobacilli |
title_sort | molecular characterization and probiotic potential of kimere lactobacilli |
topic | probiotics cabt lactobacillus cabt Kenya cabt genetics cabt characterization cabt Pennisetum glaucum cabt Perlhirse (DE-588)4209339-9 gnd Probiotikum (DE-588)4338112-1 gnd Isolierung Mikrobiologie (DE-588)4193882-3 gnd Lactobacillus (DE-588)4166370-6 gnd |
topic_facet | probiotics lactobacillus Kenya genetics characterization Pennisetum glaucum Perlhirse Probiotikum Isolierung Mikrobiologie Lactobacillus Hochschulschrift |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022272852&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
work_keys_str_mv | AT njerupatrisionjiru molecularcharacterizationandprobioticpotentialofkimerelactobacilli |