Transmission of minute virus of mice in mouse populations: international PhD program "infection biology"
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
DVG-Service
2009
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Abstract Inhaltsverzeichnis |
| Beschreibung: | [12], 93 S. Ill., graph. Darst. |
| ISBN: | 9783941703261 |
Internformat
MARC
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| 100 | 1 | |a Keubler, Lydia Maria |d 1979- |e Verfasser |0 (DE-588)138854165 |4 aut | |
| 245 | 1 | 0 | |a Transmission of minute virus of mice in mouse populations |b international PhD program "infection biology" |c by Lydia Maria Janus |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Gießen |b DVG-Service |c 2009 | |
| 300 | |a [12], 93 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a viral diseases |2 cabt | |
| 650 | 2 | |a virus of mice | |
| 650 | 7 | |a parvoviridae |2 cabt | |
| 650 | 7 | |a transmission |2 cabt | |
| 650 | 7 | |a polymerase chain reaction |2 cabt | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
ABBREVIATIONS
1 ABSTRACT
INTRODUCTION
2.1 2.2
2.3
2.4
2.5
2.6
2.7
2.8
PARVO VI RIDAE RODENT PARVOVIRUSES
MINUTE VIRUS OF MICE
2.3.1 MOUSE PARVOVIRUS
PARVOVIRAL IMPACT ON RESEARCH
DIAGNOSTIC METHODS
PARVOVIRAL PERSISTENCE
REDERIVATION
AIMS OF THIS STUDY
4
5 7 9 10
11 13
15 17
3 MATERIALS AND METHODS 19
3.1 MATERIALS 19
3.2 MOUSE STRAINS AND HUSBANDRY 19
3.3 ANIMAL EXPERIMENTS 20
3.4 VIRUS PROPAGATION AND TITRATION 20
3.5 MVMI INFECTION OF MICE 21
3.6 INDIRECT IMMUNFLUORESCENCE ASSAY (IFA) 21
3.7 ENZYME LINKED MMUNO-ABSORBENT ASSAY (ELISA) 22
3.8 MOUSE ANTIBODY PRODUCTION TEST (MAP-TEST) 23
3.9 SUBLETHAL IRRADIATION 23
3.10 DNA-EXTRACTION 24
3.11 PCR 24
3.12 SEQUENCING AND SEQUENCE ANALYSIS 26
3.13 RT-PCR 27
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/995700702
DIGITALISIERT DURCH
IMAGE 2
TABLE OF CONTENTS
3.14 REAL-TIME PCR 28
3.15 EXPIANT CULTURES 29
3.16 MAGNETIC CELL SORTING (MACS) AND ANALYSIS 30
3.17 IN SITU RT-PCR 31
3.18 OOCYTE AND 2-CELL EMBRYO COLLECTION 32
3.19 SPERM CELL COLLECTION 33
3.20 IN VITRO CONTAMINATION OF OOCYTES 33
3.21 STATISTICAL ANALYSIS 34
4 RESULTS 35
4.1 PART I: ANTIBODY RESPONSE TO MVMI IN MULTIPLE STRAINS OF MICE 35
4.1.1 SEROCONVERSION VARIED AMONG MOUSE STRAINS 35
4.1.2 SERUM LEVELS OF MVMI-SPECIFIC ANTIBODIES DEPENDED ON THE
GENETIC BACKGROUND OF MICE 36
4.1.3 TITRATION STUDIES: VIRAL LOAD DETERMINED STRAIN-DEPENDENT
SEROCONVERSION 39
4.1.4 EVALUATION OF THE INFLUENCE OF THE MHC-HAPLOTYPE ON THE
ANTIBODY RESPONSE 41
4.2 PART II: MVMI PERSISTENT INFECTION IN IMMUNOCOMPETENT MICE 43
4.2.1 DETECTION OF VIRAL DNA AT 16 WEEKS POST EXPOSURE 43
4.2.2 USE OF MOUSE ANTIBODY PRODUCTION TEST TO DETERMINE VIRAL
INFECTIVITY 44
4.2.3 FECAL SHEDDING OF MVMI IN FVB/N MICE 44
4.2.4 DETECTION OF VIRAL DNA AND MRNA AT 8 WEEKS POST INOCULATION 46
4.2.5 USE OF EXPIANT CULTURES TO DETERMINE VIRAL INFECTIVITY 47
4.2.6 USE OF MACS ANALYSIS AND IN SITU RT-PCR TO IDENTIFY SPLEEN CELLS
SUPPORTING MVMI PERSISTENCE 48
4.2.7 IMMUNOSUPPRESSION OF PERSISTENTLY INFECTED MICE BY IRRADIATION 51
4.3 PART III: RISK ASSESSMENT OF MVMI TRANSMISSION DURING REDERIVATION
53
4.3.1 DETECTION OF VIRAL DNA IN REPRODUCTIVE ORGANS 53
4.3.2 DETECTION OF MVMI DNA IN OOCYTES 55
IMAGE 3
TABLE OF CONTENTS
4.3.3 DETECTION OF MVMI DNA IN SPERM CELLS 56
4.3.4 DETECTION OF MVMI DNA IN 2-CELL EMBRYOS 57
4.3.5 IN VITRO CONTAMINATION OF OOCYTES 58
5 DISCUSSION
6 OUTLOOK
7 APPENDIX
59
69
71
7.1 LABORATORY INSTRUMENTS 71
7.2 COMPOSITION OF BUFFERS, SOLUTIONS AND MEDIA 72
7.3 SUPPORTIVE DATA 73
8 REFERENCES 76
CURRICULUM VITAE 87
PUBLICATIONS 89
ACKNOWLEDGEMENTS 91
DECLARATION 93
|
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| discipline | Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Keubler, Lydia Maria 1979- Verfasser (DE-588)138854165 aut Transmission of minute virus of mice in mouse populations international PhD program "infection biology" by Lydia Maria Janus 1. Aufl. Gießen DVG-Service 2009 [12], 93 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Hannover, Med. Hochsch., Diss., 2009 mice cabt viral diseases cabt virus of mice parvoviridae cabt transmission cabt polymerase chain reaction cabt (DE-588)4113937-9 Hochschulschrift gnd-content http://library.vetmed.fu-berlin.de/ResourceList/details/172404 Abstract DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022261752&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Keubler, Lydia Maria 1979- Transmission of minute virus of mice in mouse populations international PhD program "infection biology" mice cabt viral diseases cabt virus of mice parvoviridae cabt transmission cabt polymerase chain reaction cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" |
| title_auth | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" |
| title_exact_search | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" |
| title_full | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" by Lydia Maria Janus |
| title_fullStr | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" by Lydia Maria Janus |
| title_full_unstemmed | Transmission of minute virus of mice in mouse populations international PhD program "infection biology" by Lydia Maria Janus |
| title_short | Transmission of minute virus of mice in mouse populations |
| title_sort | transmission of minute virus of mice in mouse populations international phd program infection biology |
| title_sub | international PhD program "infection biology" |
| topic | mice cabt viral diseases cabt virus of mice parvoviridae cabt transmission cabt polymerase chain reaction cabt |
| topic_facet | mice viral diseases virus of mice parvoviridae transmission polymerase chain reaction Hochschulschrift |
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