Dictyostelium discoideum protocols:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Schriftenreihe: | Methods in molecular biology
346 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 564 S. Ill., graph. Darst. |
| ISBN: | 1588296237 |
Internformat
MARC
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| 245 | 1 | 0 | |a Dictyostelium discoideum protocols |c ed. by Ludwig Eichinger ... |
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Datensatz im Suchindex
| _version_ | 1804145213120184320 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I The Organism, Community Resources,
and Genome-wide Studies
1 The Secret Lives of Dictyostelium
Richard H. Kessin 3
2 The Genome of Dictyostelium discoideum
Adam Kuspa and William F. Loomis 15
3 The cDNA Sequencing Project
Hideko Urushihara, Takahiro Mono, and Yoshimasa Tanaka 31
4 dictyBase and the Dicty Stock Center
Petra Fey, Pascale Gaudet, Karen E. Pilcher,
Jakob Franke, and Rex L Chisholm 51
5 Analysis of Gene Expression Using cDNA Microarrays
Marcel Kaul and Ludwig Eichinger 75
6 Proteomic Analysis of Dictyostelium discoideum
Udo Roth, Stefan Miiller, and Franz-Georg Hanisch 95
Part II Basic Methods and Molecular Genetic Techniques
7 Cultivation, Spore Production, and Mating
Hideko Urushihara 113
8 Parasexual Genetics Using Axenic Cells
Jason King and Robert Insall 125
9 Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior
Paul R. Fisher and Sarah J. Annesley 137
10 Purification Techniques of Subcellular Compartments
for Analytical and Preparative Purposes
Laurence Aubry and Gerard Klein 171
11 Generation of Multiple Knockout Mutants Using
the Cre-loxP System
Alan R. Kimmel and Jan Faix 787
12 Restriction Enzyme-Mediated Integration (REMI) Mutagenesis
Adam Kuspa 201
vii
viii Contents
13 RNA Interference and Antisense-Mediated Gene Silencing
in Dictyostelium
Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen 211
Part III Imaging and Localization Methods
14 Application of Fluorescent Protein Tags as Reporters in Live-Cell
Imaging Studies
Annette Miiller-Taubenberger 229
15 Investigating Gene Expression: In Situ Hybridization
and Reporter Genes
Ricardo Escalante and Leandro Sastre 247
16 Application of 2D and 3D DIAS to Motion Analysis of Live Cells
in Transmission and Confocal Microscopy Imaging
Deborah Wessels, Spencer Kuhl, and David R. Soil 261
1 7 Using Quantitative Fluorescence Microscopy and FRET Imaging
to Measure Spatiotemporal Signaling Events
in Single Living Cells
Xuehua Xu, Joseph A. Brzostowski, and Tian fin 281
18 Visualizing Signaling and Cell Movement During
the Multicellular Stages of Dictyostelium Development
Dirk Dormann and Cornells J. Weijer 297
19 Under-Agarose Chemotaxis of Dictyostelium discoideum
David Woznica and David A. Knecht 311
20 Optimized Fixation and Immunofluorescence Staining Methods
for Dictyostelium Cells
Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati 327
21 Cryofixation Methods for Ultrastructural Studies
of Dictyostelium discoideum
Mark f. Crimson and Richard L. Blanton 339
Part IV Dictyostelium as Model Organism
22 Analysis of Signal Transduction: Formation of cAMP, cGMP,
and lns(1,4,5)P3 In Vivo and In Vitro
Peter }. M. Van Haastert 369
23 Assaying Chemotaxis of Dictyostelium Cells
Michelle C. Mendoza and Richard A. Firtel 393
24 Characterization of Cross-Linked Actin Filament Gels and Bundles
Using Birefringence and Polarized Light Scattering
Ruth Furukawa and Marcus Fechheimer 407
Contents ix
25 Quantitative and Microscopic Methods for Studying
the Endocytic Pathway
Francisco Rivero and Markus Maniak 423
26 Preparation of Intact, Highly Purified Phagosomes
from Dictyostelium
Daniel Gotthardt, Regis Dieckmann, Vincent Blancheteau,
Claudia Kistler, Frank Reichardt, and Thierry Soldati 439
27 Assaying Cell-Cell Adhesion
Salvatore Bozzaro 449
28 Periodic Activation of ERK2 and Partial Involvement of G Protein
in ERK2 Activation by cAMP in Dictyostelium Cells
Mineko Maeda 469
29 An Improved Method for Dictyostelium Centrosome Isolation
Irene Schulz, Yvonne Reinders, Albert Sickmann,
and Ralph Graf 479
30 Epigenetics in Dictyostelium
Markus Kaller, Wolfgang Nellen, and Jonathan R. Chubb 491
31 Dictyostelium discoideum as a Model to Study Host-Pathogen
Interactions
Can Unal and Michael Steinert 507
32 Pharmacogenetics: Defining the Genetic Basis of Drug Action
and Inositol Trisphosphate Analysis
Kathryn E. Adley, Melanie Keim, and Robin S. B.Williams 517
33 How to Assess and Study Cell Death in Dictyostelium discoideum
Artemis Kosta, Catherine Laporte, David Lam, Emilie Tresse,
Marie-Francoise Luciani, and Pierre Golstein 535
Index 551
|
| any_adam_object | 1 |
| author2 | Eichinger, Ludwig |
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| author_facet | Eichinger, Ludwig |
| building | Verbundindex |
| bvnumber | BV026613754 |
| ctrlnum | (OCoLC)699130737 (DE-599)GBV503639974 |
| dewey-full | 579.52 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579.52 |
| dewey-search | 579.52 |
| dewey-sort | 3579.52 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| isbn | 1588296237 |
| language | English |
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| physical | XV, 564 S. Ill., graph. Darst. |
| publishDate | 2006 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... Totowa, NJ Humana Press 2006 XV, 564 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 346 Dictyostelium discoideum (DE-588)4012135-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Dictyostelium discoideum (DE-588)4012135-5 s DE-188 Eichinger, Ludwig edt Methods in molecular biology 346 (DE-604)BV035362695 346 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022168108&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dictyostelium discoideum protocols Methods in molecular biology Dictyostelium discoideum (DE-588)4012135-5 gnd |
| subject_GND | (DE-588)4012135-5 (DE-588)4143413-4 |
| title | Dictyostelium discoideum protocols |
| title_auth | Dictyostelium discoideum protocols |
| title_exact_search | Dictyostelium discoideum protocols |
| title_full | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_fullStr | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_full_unstemmed | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_short | Dictyostelium discoideum protocols |
| title_sort | dictyostelium discoideum protocols |
| topic | Dictyostelium discoideum (DE-588)4012135-5 gnd |
| topic_facet | Dictyostelium discoideum Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022168108&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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