Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.):
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 109 S. Ill., graph. Darst. |
Internformat
MARC
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| 001 | BV026536379 | ||
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| 008 | 110326s2005 ad|| m||| 00||| eng d | ||
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| 100 | 1 | |a Trujillo, Marco |d 1974- |e Verfasser |0 (DE-588)130191280 |4 aut | |
| 245 | 1 | 0 | |a Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) |c submitted by Marco Trujillo Linke |
| 264 | 1 | |c 2005 | |
| 300 | |a VI, 109 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Gießen, Univ., Diss., 2005 | ||
| 650 | 0 | 7 | |a Wechselwirkung |0 (DE-588)4064937-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Erysiphe graminis |0 (DE-588)4152953-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Getreide |0 (DE-588)4121356-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Getreide |0 (DE-588)4121356-7 |D s |
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| 689 | 0 | 2 | |a Wechselwirkung |0 (DE-588)4064937-4 |D s |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022100648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-022100648 | |
Datensatz im Suchindex
| _version_ | 1808225622186524672 |
|---|---|
| adam_text |
1.
INTRODUCTION
1
1.1.
COMPATIBILITY
1
1.2.
RESISTANCE
1
1.2.1.
NON-HOST
RESISTANCE
2
1.2.2.
HOST
RESISTANCE
3
1.2.2.1
.THE
NON-RACE-SPECIFIC
RESISTANCE
4
1.2.2.2.THE
RACE-SPECIFIC
RESISTANCE
4
1
.2.2.3.INDUCED
RESISTANCE
5
1.3.
DEFENCE
MECHANISMS
6
1.3.1.
HYPERSENSITIVE
RESPONSE
6
1.3.2.
CELL
WALL
APPOSITIONS
7
1.3.3.
PR-PROTEINS
7
1.4.
MECHANISMS
OF
PATHOGEN
RECOGNITION
AND
SIGNAL
TRANSDUCTION
8
1.5.
REACTIVE
OXYGEN
INTERMEDIATES
(ROI)
9
1.5.1.
REACTIVE
OXYGEN
INTERMEDIATES
IN
THE
HEALTHY
PLANT
10
1.5.2.
REACTIVE
OXYGEN
INTERMEDIATES
IN
PLANT
DEFENCE
12
1.5.3.
THE
PLANT
NADPH
OXIDASE
GENE
14
(RESPIRATORY
BURST
OXIDASE
HOMOLOGUE)
1.6.
THE
BARLEY-POWDERY
MILDEW
PATHOSYSTEM
16
1.6.1.
THE
COMPATIBLE
INTERACTION
17
1.6.2.
THE
MLO
MEDIATED
RESISTANCE
19
1.6.3.
THE
MLA
MEDIATED
RESISTANCE
20
1.7.
OBJECTIVES
22
2.
MATERIALS
AND
METHODS
23
2.1.
PLANTS,
PATHOGENS
AND
INOCULATION
23
2.2.
MICROSCOPY
OF
INFECTION
STRUCTURES
AND
HISTOCHEMICAL
STAINING
24
2.3.
RNA
EXTRACTION
AND
REVERSE
TRANSCRIPTION
25
2.4.
ISOLATION
OF
HVRBOHA
PARTIAL
CLONES
AND
FRAGMENTS
25
USED
FOR
DSRNA
SYNTHESIS
2.5.
SCREENING
OF
BAC-LIBRARIES
26
2.6.
REAL
TIME
QUANTITATIVE
RT-PCR
26
2.7.
TRANSIENT
TRANSFORMATION
ASSAY
AND
RNAI
27
3.
RESULTS
28
3.1.
CYTOLOGICAL
ANALYSIS
OF
CELLULAR
RESPONSES
IN
THE
28
NON-HOST INTERACTION
OF
DIFFERENT
BARLEY
GENOTYPES
AND
BLUMERIA
GRAMINIS
F.SP.
TRITICI
3.1.1.
QUALITATIVE
ANALYSIS
OF
THE
BARLEY-FIG/
INTERACTION
28
3.1.2.
QUANTITATIVE
ANALYSIS
OF
THE
INTERACTION
OF
BARLEY
29
LINES
SUSPMUR,
SUSPTRIT
AND
TRIGO
BIASA
WITH
BGT
3.1.3.
ANALYSIS
OF
THE
FUNCTION
OF
ROR
GENES
IN
NON-HOST
31
PENETRATION
RESISTANCE
3.1.4.
ANALYSIS
OF
THE
FUNCTION
OF
THE
RAR1
GENE
IN
BARLEY
33
NON-HOST
RESISTANCE
TO
2?GTA95
3.2.
HISTOCHEMICAL
ANALYSIS
OF
SUPEROXIDE
(O2*
'
)
AND
HYDROGEN
PEROXIDE
35
(H2O2)
PRODUCTION
DURING
THE
NON-HOST
INTERACTION
OF
BARLEY
AND
WHEAT
WITH
INAPPROPRIATE
FORMAE
SPECIALES
OF
BLUMERIA
GRAMINIS
3.2.1.
INTERACTION
PHENOTYPES
OF
BARLEY
AND
WHEAT
WITH
3
5
THE
INAPPROPRIATE
FORMAE
SPECIALES
OF
B.
GRAMINIS
3.2.2.
CELLULAR
LOCALIZATION
OF
O2''
37
3.2.3.
SIMULTANEOUS
DETECTION
OF
O2'"
AND
H2O2
39
3.2.4.
QUANTITATIVE
ANALYSIS
OF
O2''
DETECTION
40
3.2.5.
QUANTITATIVE
ANALYSIS
OF
SIMULTANEOUS
41
O2'
AND
H2O2
DETECTION
3.3.
CHARACTERIZATION
OF
A
BARLEY
RESPIRATORY
BURST
OXIDASE
42
HOMOLOGUE
IN
THE
INTERACTION
WITH
BLUMERIA
GRAMINIS
3.3.1.
CYTOLOGICAL
ANALYSIS
OF
ROI
PRODUCTION
42
3.3.2.
ISOLATION
OF
HVRBOHA
44
3.3.3.
FUNCTIONAL
ANALYSIS
OF
HVRBOHA
47
3.3.4.
EXPRESSION
ANALYSIS
OF
HVRBOHA
49
4.
DISCUSSION
51
4.1.
CYTOLOGICAL
AND
GENETIC
OVERLAP
OF
BARLEY
HOST
AND
51
NON-HOST
RESISTANCE
TO
BLUMERIA
GRAMINIS
4.1.1.
CELLULAR
DEFENCE
RESPONSES
OF
THE
NON-HOST
RESISTANCE
51
4.1.2.
INVOLVEMENT
OF
ROR1/ROR2
IN
NON-HOST
RESISTANCE
53
4.1.3.
INVOLVEMENT
OF
RAR
1
IN
NON-HOST
RESISTANCE
5
5
4.2.
SUPEROXIDE
AND
HYDROGEN
PEROXIDE
PLAY
DIFFERENT
ROLES
IN
THE
57
NON-HOST
INTERACTION
OF
BARLEY
AND
WHEAT
WITH
INAPPROPRIATE
FORMAE
SPECIALES
OF
BLUMERIA
GRAMINIS
4.2.1.
O2*'
PRODUCTION
IN
THE
NON-HOST
INTERACTION
57
4.2.2.
H2O2
PRODUCTION
IN
THE
NON-HOST
INTERACTION
60
4.2.3.
INTERPLAY
OF
O2
'
'
AND
H2O2
62
4.2.4.
THE
ROLE
OF
THE
RAC
PROTEINS
IN
NON-HOST
RESISTANCE
64
4.3.
THE
BARLEY
RESPIRATORY
BURST
OXIDASE
HOMOLOGUE
IN
THE
66
INTERACTION
WITH
BLUMERIA
GRAMINIS'.
FRIEND
OR
FOE?
4.3.1.
TRANSIENT
KNOCK-DOWN
OF
HVRBOHA
66
4.3.2.
SEQUENCE
DEPENDENT
EFFICIENCY
OF
HVRBOHA
68
TRANSIENT
KNOCK-DOWN
4.3.3.
RBOH
AS
A
SOURCE
OF
ROI
AND
THEIR
POSSIBLE
ROLES
69
4.3.4.
EVOLUTIONARY
CONSEQUENCES
FOR
RBOH
FUNCTION
71
5.
SUMMARY
/
ZUSAMMENFASSUNG
74
6.
REFERENCES
76
7.
ANNEXES
96
VI |
| any_adam_object | 1 |
| author | Trujillo, Marco 1974- |
| author_GND | (DE-588)130191280 |
| author_facet | Trujillo, Marco 1974- |
| author_role | aut |
| author_sort | Trujillo, Marco 1974- |
| author_variant | m t mt |
| building | Verbundindex |
| bvnumber | BV026536379 |
| ctrlnum | (OCoLC)615397456 (DE-599)BVBBV026536379 |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| id | DE-604.BV026536379 |
| illustrated | Illustrated |
| indexdate | 2024-08-24T00:12:12Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022100648 |
| oclc_num | 615397456 |
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| owner | DE-188 |
| owner_facet | DE-188 |
| physical | VI, 109 S. Ill., graph. Darst. |
| publishDate | 2005 |
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| spelling | Trujillo, Marco 1974- Verfasser (DE-588)130191280 aut Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) submitted by Marco Trujillo Linke 2005 VI, 109 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Gießen, Univ., Diss., 2005 Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Erysiphe graminis (DE-588)4152953-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Getreide (DE-588)4121356-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Getreide (DE-588)4121356-7 s Erysiphe graminis (DE-588)4152953-4 s Wechselwirkung (DE-588)4064937-4 s Molekularbiologie (DE-588)4039983-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022100648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Trujillo, Marco 1974- Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) Wechselwirkung (DE-588)4064937-4 gnd Erysiphe graminis (DE-588)4152953-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Getreide (DE-588)4121356-7 gnd |
| subject_GND | (DE-588)4064937-4 (DE-588)4152953-4 (DE-588)4039983-7 (DE-588)4121356-7 (DE-588)4113937-9 |
| title | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) |
| title_auth | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) |
| title_exact_search | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) |
| title_full | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) submitted by Marco Trujillo Linke |
| title_fullStr | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) submitted by Marco Trujillo Linke |
| title_full_unstemmed | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) submitted by Marco Trujillo Linke |
| title_short | Cytological and molecular analysis of host and non-host interactions of cereals with the powdery mildew fungus (Blumeria graminis ff. spp.) |
| title_sort | cytological and molecular analysis of host and non host interactions of cereals with the powdery mildew fungus blumeria graminis ff spp |
| topic | Wechselwirkung (DE-588)4064937-4 gnd Erysiphe graminis (DE-588)4152953-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Getreide (DE-588)4121356-7 gnd |
| topic_facet | Wechselwirkung Erysiphe graminis Molekularbiologie Getreide Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022100648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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