Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 32 S. graph. Darst. |
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MARC
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| 100 | 1 | |a Hüssy, Daniela Anita |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |c vorgelegt von Daniela Anita Hüssy |
| 264 | 1 | |c 2000 | |
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| 502 | |a Zürich, Univ., Diss. | ||
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Datensatz im Suchindex
| _version_ | 1804144888039604224 |
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| adam_text | Titel: Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) rep
Autor: Hüssy, Daniela Anita
Jahr: 2000
INHALTSVERZEICHNIS
1. ABSTRACT................................................................................1
2. EVTRODUCTION.......................................................................2
3. MATERIALS AND METHODS....................................................4
3.1. Samples.............................................................................4
3.2. Sample preparation............................................................5
3.3. DNA extraction..................................................................5
3.4. Conventional seminested PCR.............................................6
3.5. Primers andprobes for fluorogenic PCRs.............................6
3.6. Plasmid Standards for quantitation.......................................7
a) Standard for the Ovine herpesvirus 2 (OvHV-2)...............7
b) Standard for the genomic sheep DNA (SHgenom)..............8
3.7. Fluorogenic PCRs..............................................................8
a) OvHV-2.......................................................................8
b) SHgenom......................................................................9
3.8. Relative quantitation..........................................................9
3.9. OvHV-2 replication in vivo................................................10
4.RESULTS..................................................................................10
4.1. Comparison ofthe OvHV-2 fluorogenic PCR to the
conventional seminested PCR............................................10
4.2. Detection of genomic sheep DNA bv fluorogenic PCR...........11
4.3. Analytical sensitivity. linearity and efficiency.......................12
4.4. Reproducibility of the two fluorogenic PCRs........................15
4.5. Example for relative quantitation of OvHV-2.......................15
5. DISCUSSION............................................................................17
6.ACKNOWLEDGMENTS............................................................20
7. REFERENCES...........................................................................21
8.FIGURELEGENDSANDFIGURES............................................23
9. TABLES....................................................................................29
DANK...........................................................................................31
LEBENSLAUF...............................................................................32
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| spelling | Hüssy, Daniela Anita Verfasser aut Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep vorgelegt von Daniela Anita Hüssy 2000 32 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zürich, Univ., Diss. (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021953332&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hüssy, Daniela Anita Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |
| subject_GND | (DE-588)4113937-9 |
| title | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |
| title_auth | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |
| title_exact_search | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |
| title_full | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep vorgelegt von Daniela Anita Hüssy |
| title_fullStr | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep vorgelegt von Daniela Anita Hüssy |
| title_full_unstemmed | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep vorgelegt von Daniela Anita Hüssy |
| title_short | Establishment of a quantitative fluorogenic PCR assay for measuring ovine herpesvirus 2 (OVHV-2) replication in sheep |
| title_sort | establishment of a quantitative fluorogenic pcr assay for measuring ovine herpesvirus 2 ovhv 2 replication in sheep |
| topic_facet | Hochschulschrift |
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