Beta-Lactoglobulin: denaturation and aggregation = Beta-Lactoglobuline
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
1997
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IV, 135 S. graph. Darst. |
| ISBN: | 9039311307 |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV026167481 | ||
| 003 | DE-604 | ||
| 005 | 20180613 | ||
| 007 | t | ||
| 008 | 110326s1997 d||| m||| 00||| eng d | ||
| 020 | |a 9039311307 |9 90-393-1130-7 | ||
| 035 | |a (OCoLC)917404440 | ||
| 035 | |a (DE-599)BVBBV026167481 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-188 | ||
| 100 | 1 | |a Hoffmann, Marion |d 1967- |e Verfasser |0 (DE-588)173400930 |4 aut | |
| 245 | 1 | 0 | |a Beta-Lactoglobulin |b denaturation and aggregation = Beta-Lactoglobuline |c door Marion Amanda Maria Hoffmann |
| 246 | 1 | 1 | |a Beta-Lactoglobuline |
| 264 | 1 | |c 1997 | |
| 300 | |a IV, 135 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Utrecht, Univ., Diss., 1997 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021753279&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-021753279 | ||
Record in the Search Index
| _version_ | 1804144620736610304 |
|---|---|
| adam_text | Chapter 1
Introduction 1
Whey proteins 1
p-Lactoglobulin 2
Thermal behaviour of p-lactoglobulin 3
Model for the heat-induced aggregation of p-lactoglobulin 4
Purpose of the study 6
Outline of this thesis 6
References 7
Chapter 2
Heat-induced aggregation of p-lactoglobulin:
role of the free thiol group and disulphide bonds 9
Introduction 10
Materials and methods 11
Results and discussion 13
Formation of disulphide-linked aggregates 13
Aggregation of p-lactoglobulin in the presence of NEM 15
Effect of pH on the aggregation of p-lactoglobulin 18
Conclusions 22
References 23
Chapter 3
Aggregation of p-lactoglobulin studied by in situ light scattering 25
Introduction 26
Theory 27
Materials and methods 30
Results and discussion 33
Typical curves and reproducibility 33
In situ and heat-quenched light scattering 35
p-Lactoglobulin samples of different origin 36
Effect of concentration of p-lactoglobulin 39
Effect of salt concentration and composition 40
Effect of heating temperature 41
Effect of pH 43
Conclusions 44
References 45
i
Chapter 4
Molecular mass distributions of heat-induced p-lactoglobulin aggregates 47
Introduction 48
Materials and methods 49
Results and discussion 51
Typical SEC-MALLS elution profiles 51
Comparison of the molecular masses obtained by MALLS detection
and by conventional calibration 52
Decrease in concentration of native p-lactoglobulin 55
Effect of heating time and initial p-lactoglobulin concentration on
aggregate formation 57
Interactions involved in aggregate formation 60
Effect of pH, heating temperature and salt on aggregate formation 62
Conclusions 64
References 65
Chapter 5
Differential and adiabatic calorimetry study of p-lactoglobulin 67
Introduction 68
Materials and methods 69
Results and discussion 71
Typical DSC thermograms 71
Dependence of Tp on scan rate 72
Conclusions 73
References 73
Chapter 6
Isothermal and scanning calorimetry measurements on p-lactoglobulin 75
Introduction 76
Theory 78
Materials and methods 80
Results and discussion 82
Typical curves and correction of the thermograms 82
Evaluation of core parameter set at 65 °C 83
Concentration dependence at 65 °C 85
Temperature dependence 86
Conclusions 90
References 91
ii
Chapter 7
Heat-induced p-lactoglobulin aggregates: influence of phi 93
Introduction 94
Materials and methods 96
Results and discussion 98
Decrease in concentration of native p-lactoglobulin 98
SEC-MALLS elution profiles 99
Interactions involved in aggregate formation 102
Exposure and disappearance of reactive thiol groups 103
Isothermal calorimetry experiments 106
Conclusions 109
References 109
Chapter 8
General discussion 111
Introduction 111
Denaturation and aggregation in water at near-neutral pH
and the role of the thiol group 111
Model for the denaturation and aggregation in water at near-neutral pH 112
Temperature dependence 115
Influence of pH 116
Influence of salt concentration 117
Concluding remarks and relevance of the results obtained 118
References 119
Summary 121
Samenvatting 127
Dankwoord 133
Curriculum Vitae 135
iii
|
| any_adam_object | 1 |
| author | Hoffmann, Marion 1967- |
| author_GND | (DE-588)173400930 |
| author_facet | Hoffmann, Marion 1967- |
| author_role | aut |
| author_sort | Hoffmann, Marion 1967- |
| author_variant | m h mh |
| building | Verbundindex |
| bvnumber | BV026167481 |
| ctrlnum | (OCoLC)917404440 (DE-599)BVBBV026167481 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01151nam a2200301 c 4500</leader><controlfield tag="001">BV026167481</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180613 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110326s1997 d||| m||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9039311307</subfield><subfield code="9">90-393-1130-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)917404440</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV026167481</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hoffmann, Marion</subfield><subfield code="d">1967-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)173400930</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Beta-Lactoglobulin</subfield><subfield code="b">denaturation and aggregation = Beta-Lactoglobuline</subfield><subfield code="c">door Marion Amanda Maria Hoffmann</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Beta-Lactoglobuline</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">IV, 135 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Utrecht, Univ., Diss., 1997</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021753279&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-021753279</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV026167481 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:06:27Z |
| institution | BVB |
| isbn | 9039311307 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-021753279 |
| oclc_num | 917404440 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | IV, 135 S. graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Hoffmann, Marion 1967- Verfasser (DE-588)173400930 aut Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline door Marion Amanda Maria Hoffmann Beta-Lactoglobuline 1997 IV, 135 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Utrecht, Univ., Diss., 1997 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021753279&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hoffmann, Marion 1967- Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline |
| subject_GND | (DE-588)4113937-9 |
| title | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline |
| title_alt | Beta-Lactoglobuline |
| title_auth | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline |
| title_exact_search | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline |
| title_full | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline door Marion Amanda Maria Hoffmann |
| title_fullStr | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline door Marion Amanda Maria Hoffmann |
| title_full_unstemmed | Beta-Lactoglobulin denaturation and aggregation = Beta-Lactoglobuline door Marion Amanda Maria Hoffmann |
| title_short | Beta-Lactoglobulin |
| title_sort | beta lactoglobulin denaturation and aggregation beta lactoglobuline |
| title_sub | denaturation and aggregation = Beta-Lactoglobuline |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021753279&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hoffmannmarion betalactoglobulindenaturationandaggregationbetalactoglobuline AT hoffmannmarion betalactoglobuline |