Manipulating the mouse embryo: a laboratory manual
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | Undetermined |
| Veröffentlicht: |
Cold Spring Harbor, NY
Cold Spring Harbor Laboratory Press
1994
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| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 497 S. Ill., graph. Darst. |
| ISBN: | 0879693843 |
Internformat
MARC
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| 245 | 1 | 0 | |a Manipulating the mouse embryo |b a laboratory manual |c Brigid Hogan ... |
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Datensatz im Suchindex
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| adam_text | Manipulating the Mouse Embryo
A LABORATORY MANUAL
Second Edition
Brigid Hogan
Howard Hughes Medical Institute and
Vanderbilt University Medical School
Rosa Beddington
National Institute for Medical Research, London
Frank Costantini
Columbia University
Elizabeth Lacy
Memorial Sloan-Kettering Cancer Center
Cold Spring Harbor Laboratory Press
Contents
Preface to First Edition, v
Preface to Second Edition, vii
Developmental Genetics and Embryology of the Mouse: Past, Present, and
Future, 1
Mendelian Inheritance and Linkage: The Beginnings of Mouse Genetics, 2
Origins of the Laboratory Mouse, 3
Origin of Inbred Strains and Other Resources of Mouse Genetics, 7
Origins of Developmental Genetics of the Mouse, 10
Origins of Experimental Mouse Embryology, 13
Manipulating the Mouse Genome, 15
The Systematic Search for New Genes and Developmental Mutants in the
Mouse, 16
Section A SUMMARY OF MOUSE DEVELOPMENT, 19
EARLY MOUSE DEVELOPMENT, 21
Origin of Germ Cells and Their Migration to the Genital Ridges, 28
Sex Determination and Germ Cells, 31
- Spermatogenesis, 32
Oogenesis, 33
Ovulation, 38
Fertilization, 40
Cytoskeletal Organization of the Egg before and after Fertilization, 41
Parthenogenesis, 43
Early Cleavage: One-cell Embryo to Eight-cell Uncompacted Morula, 45
Analysis of Embiyonic Gene Expression Using RT-PCR Techniques and
cDNA Libraries, 47
Restriction in the Developmental Potential of Embiyonic Nuclei, 47
Compaction and the Formation of the Blastocyst: The First
Differentiation Events, 48
Changes in Cell Adhesiveness with Compaction, 48
Cell Polarization with Compaction, 49
Segregation of the Trophectoderm and Inner Cell Mass Cell Lineages, 51
Implantation, 51
Trophectoderm and Its Derivatives, 54
The Second Round of Differentiation: Formation of the Primitive
Endoderm and Ectoderm, 56
ix
x Contents
Lineage Markers Used with Mouse Embryos, 57
The Primitive Ectoderm Lineage, 57
Primitive Ectoderm Cells Divide Rapidly, 61
The Epiblast Is a Pluripotent Tissue, 62
General Description of Gastrulation and the Formation of Mesoderm
and Definitive Endoderm, 63
The Node, 68
Fate Maps of Gastrulation, 69
Generation of Regional Diversity in the Mesoderm, 73
Tail Bud, 73
Turning, 74
Somites and their Derivatives, 74
Lateral Plate and Intermediate Mesoderm: Kidney and Genital Ridges, 81
Limbs, 82
Development of the Nervous System: Neurulation, Timing of Neural
Tube Closure, 83
Generation of Regional Diversity in the Early Brain and Neural Tube, 84
Neural Crest, 88
Formation of the Branchial Arches and the Pharyngeal Region, 90
Gut, 91
Teratocarcinoma Cells and Embiyonic Stem Cells, 92
Size Regulation, 93
Imprinting, 95
X-inactivation, 96
Extraembiyonic Tissues, 98
Extraembryonic Endoderm: The Primitive Endoderm Gives Rise Only to
Visceral and Parietal Endoderm, 99
Gene Expression in Visceral Endoderm, 100
Gene Expression in Parietal Endoderm, 102
Differentiation of the Extraembiyonic Mesoderm, 103
The Structure and Function of the Placenta, 103
THE ADULT MOUSE, 106
Mouse Coat Color and Its Genetics, 106
A (agouti), 107
b (brown), 108
c (albino), 108
d (dilute), 109
Spotting Mutations, 109
Section B SETTING UP A COLONY FOR THE PRODUCTION OF
TRANSGENIC AND MUTANT MICE, 115
SETTING UP THE COLONY, 117
Production and Breeding of Transgenic and Mutant Mice, 117
Female Mice for Matings to Produce Fertilized Eggs for DNA
Injection, 117
Female Mice for Matings to Produce Blastocysts for Embiyonic
Stem Cell Injection, 120
Fertile Stud Male Mice, 120
Contents xi
Sterile Stud Male Mice for the Production of Pseudopregnant
Females, 121
Female Mice to Serve as Pseudopregnant Recipients and Foster
Mothers, 121
Transgenic Mice, Including Founder Mice and Transgenic Lines
Derived from These Founders, 123
Pathogen Control in Experimental Mouse Colonies, 124
Nomenclature for Transgenic Mice, 126
Long-term Storage of Transgenic Mice by Freezing Embryos, 126
Section C RECOVERY, CULTURE, AND TRANSFER OF EMBRYOS AND
GERM CELLS, 127
SETTING UP NATURAL MATINGS, 129
INDUCING SUPEROVULATION, 130
Influence of Age and Weight, 130
Dose of Gonadotropins, 131
Time of Administration of the Gonadotropins, 131
Strain of Mouse, 132
Reproductive Performance of the Stud Males, 132
INJECTING A MOUSE INTRAPERITONEALLY, 133
MAKING PIPETTES FOR COLLECTING AND TRANSFERRING EMBRYOS, 134
Making Pipettes from Hard Glass Capillaiy Tubing, 134
Preparing Siliconized Pasteur Pipettes, 135
RECOVERING PREIMPLANTATION EMBRYOS, 136
Opening the Abdominal Cavity, 136
Collecting Fertilized Eggs and Removing Cumulus Cells with
Hyaluronidase, 138
Collecting Two- to Eight-cell Embryos and Eight-cell Compacted
Morulae, 140
SETTING UP MICRODROP CULTURES, 142
-COLLECTING BLASTOCYSTS, 144
IN VITRO FERTILIZATION, 146
ETHANOL-INDUCED PARTHENOGENETIC ACTIVATION OF OOCYTES, 148
ISOLATING POSTIMPLANTATION-STAGE EMBRYOS, 151
Early Egg Cylinder Stage (~5 5 Days p c ), 152
Early Primitive Streak Stage (~6 5 Days p c ), 154
Late Primitive Streak Stage (-7 5 Days p c ), 156
Early Neural Fold Stage (~8 Days p c ), 157
Early Somite Stage (-8 5 Days p c ), 158
ISOLATING EXTRAEMBRYONIC MEMBRANES, 160
SEPARATING POSTIMPLANTATION GERM LAYER, 163
ISOLATING GERM CELLS FROM THE GENITAL RIDGE, 166
ISOLATING AND IN VITRO CULTURING OF IMMATURE AND PRE
OVULATORY OOCTYES, 169
VASECTOMIZING MALES AND PREPARING PSEUDOPREGNANT FEMALES,
TRANSFERRING EMBRYOS, 173
Oviduct Transfer, 173
Uterine Transfer, 178
xii Contents
CESAREAN SECTION AND FOSTERING, 182
IMPLANTING TISSUES UNDER THE KIDNEY CAPSULE, 183
TRANSFERRING OVARIES, 185
INJECTING VIRUSES AND CELLS IN UTERO, 187
Section D IN VITRO MANIPULATION OF PREIMPLANTATION
EMBRYOS, 189
REMOVING THE ZONA PELLUCIDA, 191
Method Using Acidic Tyrode Solution, 191
Method Using 0 5% Pronase Solution in M2 Medium, 191
DISAGGREGATING CLEAVAGE-STAGE EMBRYOS INTO INDIVIDUAL CELLS,
MAKING AGGREGATION CHIMERAS, 193
ISOLATING THE INNER CELL MASS BY IMMUNOSURGERY, 194
MICROINJECTING ES CELLS INTO HOST BLASTOCYSTS, 196
Preparing the Injection and Holding Pipettes, 196
Assembling the Micromanipulator, 199
Injecting Blastocysts, 201
ALTERNATIVES TO MICROINJECTING BLASTOCYSTS TO PRODUCE
CHIMERAS, 205
ELECTROFUSING TWO-CELL MOUSE EMBRYOS, 207
NUCLEAR TRANSPLANTATION IN THE MOUSE EMBRYO, 209
Isolating Embryos, 209
Making a Holding Pipette, 209
Making an Enucleation/Injection Pipette, 210
Enucleating an Egg, 213
Introducing Nuclei into Enucleated Embiyos, 215
Preparing Inactivated Sendai Virus, 216
Section E PRODUCTION OF TRANSGENIC MICE, 217
INTRODUCTION, 219
Applications of Transgenic Mouse Technology, 219
Methods for Gene Transfer into the Mouse, 220
DESIGNING TRANSGENES, 222
Effects of Prokaiyotic Vector Sequences, 222
Length of DNA Construct, 222
Cointroduction of Two Transgenes, 222
Distinguishing Expression of Transgenes and Endogenous Genes, 222
Strategies for Identifying Regulatory Sequences, 223
Reporter Genes, 223
Using Previously Identified Tissue-specific Regulatory Sequences in
Transgenic Constructs, 223
Expression of cDNAs and the Role of Introns in Transgene Expression,
Using Housekeeping Gene Promoters to Direct Ubiquitous Expression,
Transgenic Constructs for Conditional Gene Expression in the Mouse,
MICROINJECTING DNA INTO PRONUCLEI, 226
Contents xiii
Factors Affecting the Efficiency of Gene Transfer, 226
Preparing DNA Samples for Microinjection, 228
Method I: Electroelution with an Optional, but Recommended,
CsCl Centrifugation Step, 228
Method II: Isolation Using Low-melt Agarose and GELase, 230
Choice of Mouse Strain, 232
Timing of Injection, 232
Making Holding Pipettes, 232
Making Injection Pipettes, 235
The Microinjection Set-up, 237
Microscope, 237
Micromanipulators, 237
Table, 238
Syringe to Control Holding Pipette, 242
Syringe to Control Injection Pipette, 242
Variable Pressure Systems, 242
Constant Flow Systems, 243
Injection Chambers, 243
Injecting Mouse Egg Pronuclei, 244
Trouble-shooting Guide, 248
RETROVIRAL INFECTION OF PREIMPLANTATION EMBRYOS, 251
Section F ISOLATION, CULTURE, AND MANIPULATION OF EMBRYONIC
STEM CELLS, 253
CULTURING ES CELLS, 255
General Discussion of Culture Conditions for ES Cells, 255
Culture Medium, 256
Trypsin/EDTA, 257
Serum, 257
Addition of LIF, 258
Mouse Embiyo Fibroblasts as Feeder Layers, 258
STO Fibroblasts as Feeder Cells, 259
Mycoplasma Testing, 259
Preparing Mouse Embryo Fibroblasts, 260
Preparing STO or Mouse Embiyo Fibroblast Feeder Dishes, 261
Treatment with Mitomycin, 262
Treatment by Y-Irradiation, 262
CHOOSING HOST MOUSE STRAINS AND BREEDING HOMOZYGOUS
MUTANTS, 263
Pluripotential ES Cell Lines Already Available, 264
DE NOVO ISOLATION OF EMBRYONIC STEM CELLS FROM
BLASTOCYSTS, 265
OVARIECTOMY OF PREGNANT FEMALE MICE TO INDUCE IMPLANTATION
DELAY OF BLASTOCYSTS, 273
DESIGNING TARGETING CONSTRUCTS AND PREPARING DNA FOR
ELECTROPORATION, 275
General Considerations, 275
Vector Design, 275
DNA Purification, 276
xiv Contents
ELECTROPORATING DNA INTO EMBRYONIC STEM CELLS AND SELECTION
FOR CELLS IN WHICH DNA HAS UNDERGONE HOMOLOGOUS
RECOMBINATION, 277
ISOLATING INDIVIDUAL EMBRYONIC STEM CELL COLONIES, 279
RAPID PREPARATION OF DNA FROM CELLS IN 24-WELL TISSUE CULTURE
DISHES, 282
FREEZING CELLS, 283
ALTERNATIVE RAPID METHOD FOR REPLICA PLATING AND FREEZING
CELLS AND ANALYZING DNA BY SOUTHERN BLOT ASSAY USING
96-WELL TISSUE CULTURE DISHES, 285
Part A: Picking, Replicating, and Freezing Colonies, 285
Part B: Isolating and Screening DNA, 287
RAPID ANALYSIS OF DNA BY POLYMERASE CHAIN REACTION, 289
Section G ANALYSIS OF TRANSGENIC MICE, 291
ANALYZING EXOGENOUS GENES IN TRANSGENIC MICE, 293
ISOLATING HIGH-MOLECULAR-WEIGHT DNA FROM MOUSE TAILS, 296
ISOLATING HIGH-MOLECULAR-WEIGHT DNA FROM YOLK SACS OF 9 5-DAY
P C OR LATER EMBRYOS, 299
PERFORMING POLYMERASE CHAIN REACTION ON SMALL FRAGMENTS OF
MOUSE EMBRYOS, 301
IDENTIFYING HOMOZYGOUS TRANSGENIC MICE OR EMBRYOS, 305
Quantitation of Transgene Dosage, 305
Quantitation of Transgene Product or Phenotype, 306
In Situ Hybridization to Interphase Nuclei, 306
Test Breeding, 307
Southern Blot Analysis Using a Flanking Probe, 307
PCR Analysis Using a Flanking Primer, 307
CLONING TRANSGENE/HOST DNA JUNCTIONS, 309
Screening Bacteriophage Libraries with Transgene, 309
Plasmid Rescue, 310
Inverse PCR, 310
KARYOTYPING MOUSE CELLS, 311
Preparation of Cells, 313
Slide Making, 314
G-Banding, 314
MAPPING INTEGRATED GENES TO CHROMOSOMES BY IN SITU
HYBRIDIZATION, 316
RNasing Slides, 317
Hybridization, 317
Washes, 318
Autoradiography, 318
TISSUE BIOPSIES: PARTIAL HEPATECTOMY, SPLENECTOMY,
NEPHRECTOMY, AND TAIL BLEEDING, 319
Partial Hepatectomy, 319
Splenectomy, 321
Nephrectomy, 322
Tail Bleeding, 323
Contents xv
Section H TECHNIQUES FOR VISUALIZING GENES, GENE PRODUCTS,
AND SPECIALIZED CELL TYPES, 325
ISOLATING TOTAL RNA FROM MOUSE EMBRYOS OR FETAL TISSUES, 327
GENERAL TECHNIQUES FOR IN SITU HYBRIDIZATION AND
IMMUNOHISTOCHEMISTRY OF MOUSE EMBRYOS, 330
Tissue Fixation, 330
Fresh 4% Paraformaldehyde, 330
Bouin s Fixative, 330
Methanol/DMSO, 4:1, 331
Dehydration into Xylene, 331
Embedding in Wax, 331
Tricks for Handling and Embedding Small Specimens, 332
Handling Blastocysts for Fixation, 333
Cutting Sections, 333
Preparing Glass Slides and Coverslips for In Situ Hybridization, 334
Coating slides with TESPA, 335
Coating Slides with Poly-L-lysine, 335
Siliconizing Coverslips, 335
Dewaxing and Rehydrating Sections Prior to In Situ Hybridization or
Staining, 335
IMMUNOHISTOCHEMISTRY OF EMBRYO SECTIONS, 336
IMMUNOHISTOCHEMISTRY OF WHOLE MOUNT EMBRYOS, 340
IN SITU HYBRIDIZATION WITH RNA PROBES, 344
General Procedures for Avoiding Contamination with RNase, 344
Fixing, Wax Embedding, and Sectioning, 344
Preparing Probe, 347
Prehybridization, 348
Hybridization, 349
Posthybridization Washing, 349
Autoradiography, 350
— Autoradiography—Developing, 350
IN SITU HYBRIDIZATION OF WHOLE MOUNT EMBRYOS WITH RNA PROBES,
General Considerations, 353
Preparing Embiyos, 353
Preparing Probe, 354
In Situ Hybridization Procedure, 357
SECTIONING SPECIMENS AFTER IN SITU HYBRIDIZATION, 368
DNA-DNA IN SITU HYBRIDIZATION TECHNIQUE FOR DETECTING
TRANSGENIC CELLS CARRYING MULTIPLE COPIES OF A
GLOBIN GENE, 369
Preparing Biotinylated Probe, 371
Preparing Tissues and Slides, 371
Hybridization, 372
Detection, 372
STAINING FOR (J-GALACTOSIDASE (lacZ) ACTIVITY, 373
Staining Whole Embiyos, 374
Staining Frozen Sections, 375
GLUCOSE PHOSPHATE ISOMERASE ELECTROPHORESIS, 376
xvi Contents
STAINING EMBRYOS FOR CARTILAGE AND BONE, 379
STAINING NEWBORN AND ADULT MICE FOR BONE, 381
DETERMINATION OF SEX USING MOLECULAR PROBES, 382
Determining Sex Using Retroviral DNA Probes, 382
Determining Sex Using Polymerase Chain Reaction to Detect the Sry
Gene, 382
SEXING EMBRYOS BY STAINING AMNION NUCLEI, 384
Section I IN VITRO CULTURE OF EGGS, EMBRYOS, PRIMORDIAL GERM
CELLS, AND TERATOCARCINOMA CELLS, 385
GENERAL CONSIDERATIONS, 387
CULTURE MEDIA FOR PREIMPLANTATION-STAGE EMBRYOS, 389
Preparing M16 Culture Medium, 390
Preparing M2 Culture Medium, 392
Preparing M2 and M16 Media from Concentrated Stocks, 394
M2 from Concentrated Stocks, 396
M16 from Concentrated Stocks, 397
Other Media Used for Culturing Preimplantation Embiyos, 397
WHITTINGHAM S MEDIUM FOR IN VITRO FERTILIZATION OF MOUSE
OOCYTES, 398
CULTURE MEDIA FOR BLASTOCYSTS, POSTIMPLANTATION EMBRYOS, AND
TISSUES, 399
Culture of Blastocysts, 399
Preparing Human Placental Cord Serum, 399
CULTURE OF PRIMITIVE STREAK/EARLY SOMITE STAGE EMBRYOS, 401
Roller Culture Apparatus, 401
Preparing Embiyos, 401
Preparing Medium, 402
CULTURE OF PRIMORDIAL GERM CELLS FROM EMBRYOS OF DIFFERENT
STAGES, 405
CULTURE OF TERATOCARCINOMA CELLS, 408
F9 Teratocarcinoma Cells, 408
Preparing Gelatin-coated Tissue Culture Dishes, 408
Subculturing with Tiypsin/EDTA, 408
Differentiation into Parietal Endoderm-like Cells, 410
Differentiation into Visceral Endoderm-like Cells, 412
Other Teratocarcinoma Cell Lines, 412
Serum-free Media for Teratocarcinoma Cells, 412
Appendix 1 Buffers and Solutions, 415
Acidic Tyrode Solution for Removing Zonae, 415
Alkaline Phosphatase Buffers, 415
Alkaline Phosphatase Staining Solution, 416
Avertin, Anesthetic, 416
Bovine Serum Albumin (BSA), 417
Ca++/Mg++-free PBS, 417
Ca++/Mg++-free Tyrode Ringer s Saline (pH 7 6-7 7), 417
Hyaluronidase, 417
Contents xvii
NTM Buffer (NTMT without Tween 20) for In Situ Hybridization of Intact
Embiyos with RNA Probes, 417
NTMT Buffer for In Situ Hybridization of Intact Embiyos with RNA Probes,
Pancreatin/Tiypsin Solution for Separating Germ and Tissue Layers, 418
PBSMT for Immunohistochemistiy of Whole Mount Embryos, 418
PBT, 418
Phenol/Chloroform Solution for Isolating Total RNA from Mouse Embryos or
Fetal Tissues, 418
Phosphate-buffered Saline (PBS), 419
Pronase Solution, 419
Saline/EDTA Buffer plus Glucose for Isolation of Germ Cells and Tissue
Culture, 419
20X SSC, 420
20X SSPE, 420
TE (Tris/EDTA) Buffer, 420
0 25% Tiypsin in Tris-Saline for Tissue Culture, 420
Tiypsin/EDTA Solutions, 421
Appendix 2 Sources of Information, 423
Genetic Resources, 423
Books, 424
Journals, 424
Databases, 425
Software, 426
Appendix 3 Suppliers, 429
Inbred, Hybrid, and Random-bred Mice, 429
Mouse Genomic DNAs and RNAs, 429
— Mouse Genomic and cDNA Libraries and Clones, 429
Equipment and Supplies, 430
Recommended Reading, 439
References, 441
Glossaiy, 471
Index, 475
|
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| language | Undetermined |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-021736203 |
| oclc_num | 611333141 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | XVII, 497 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | marc |
| spelling | Manipulating the mouse embryo a laboratory manual Brigid Hogan ... 2. ed. Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press 1994 XVII, 497 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Mice cabt Laboratory Animals cabt Genetic Engineering cabt Transgenics cabt Embryo (DE-588)4014555-4 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Embryologische Methode gnd rswk-swf Maus (DE-588)4169148-9 s Embryologische Methode f DE-604 Embryo (DE-588)4014555-4 s Hogan, Brigid Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021736203&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Manipulating the mouse embryo a laboratory manual Mice cabt Laboratory Animals cabt Genetic Engineering cabt Transgenics cabt Embryo (DE-588)4014555-4 gnd Maus (DE-588)4169148-9 gnd |
| subject_GND | (DE-588)4014555-4 (DE-588)4169148-9 |
| title | Manipulating the mouse embryo a laboratory manual |
| title_auth | Manipulating the mouse embryo a laboratory manual |
| title_exact_search | Manipulating the mouse embryo a laboratory manual |
| title_full | Manipulating the mouse embryo a laboratory manual Brigid Hogan ... |
| title_fullStr | Manipulating the mouse embryo a laboratory manual Brigid Hogan ... |
| title_full_unstemmed | Manipulating the mouse embryo a laboratory manual Brigid Hogan ... |
| title_short | Manipulating the mouse embryo |
| title_sort | manipulating the mouse embryo a laboratory manual |
| title_sub | a laboratory manual |
| topic | Mice cabt Laboratory Animals cabt Genetic Engineering cabt Transgenics cabt Embryo (DE-588)4014555-4 gnd Maus (DE-588)4169148-9 gnd |
| topic_facet | Mice Laboratory Animals Genetic Engineering Transgenics Embryo Maus Embryologische Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021736203&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hoganbrigid manipulatingthemouseembryoalaboratorymanual |