Fluorescence spectroscopy: new methods and applications
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest
Springer
1993
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Online-Zugang: | Inhaltsverzeichnis |
ISBN: | 3540552812 0387552812 |
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245 | 1 | 0 | |a Fluorescence spectroscopy |b new methods and applications |c ed. by Otto S. Wolfbeis |
264 | 1 | |a Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest |b Springer |c 1993 | |
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adam_text |
CONTENTS
PART 1. NEW METHODS IN FLUORESCENCE SPECTROSCOPY
1. FLUORESCENCE SPECTROSCOPY: WHERE WE ARE AND WHERE WE'RE GOING
BY STEVEN G. SCHULMAN 3
1 INTRODUCTION 3
2 THE MARRIAGE OF LUMINESCENCE SPECTROSCOPY WITH SEPARATION
METHODS 4
3 IMPROVEMENT OF SELECTIVITY BASED UPON NARROW-BAND
EXCITATION 6
3.1 FLUORESCENCE LINE NARROWING (FLN) SPECTROSCOPY .
.
. 6
3.2 SHPOL'SKII SPECTROSCOPY 7
3.3 MATRIX ISOLATION FLUORESCENCE SPECTROSCOPY 8
3.4 SUPERSONIC JET SPECTROSCOPY 8
4 FIBER OPTIC SENSORS - NON-DESTRUCTIVE SPECTROSCOPY .
.
.
. 9
5 SEMICONDUCTOR LASERS 10
6 REFERENCES 11
2. INTERACTIONS AND KINETICS OF SINGLE MOLECULES AS OBSERVED BY
FLUORESCENCE CORRELATION SPECTROSCOPY
BY RUDOLF RIGLER, JERKER WIDENGREN AND OLO METS 13
1 INTRODUCTION 13
2 THE EXPERIMENTAL SET UP 13
3 THEORETICAL BACKGROUND 15
4 RESULTS 17
4.1 TRANSLATIONAL DIFFUSION 17
4.2 ROTATIONAL DIFFUSION 18
4.3 MOLECULAR INTERACTIONS AND KINETICS 19
4.4 SINGLE MOLECULE EVENTS 20
5 CONCLUSIONS 21
6 REFERENCES 23
3. FAST OPTICAL IMAGING TECHNIQUES
BY CRAIG D. MACKAY 25
HTTP://D-NB.INFO/921068808
X CONTENTS
1 INTRODUCTION 25
2 HIGH SPEED IMAGING TECHNOLOGIES 25
2.1 RESOLUTION 25
2.2 SENSITIVITY 26
2.3 DYNAMIC RANGE 26
3 IMAGING SYSTEM COMPONENTS 26
4 EXAMPLES OF DETECTOR SYSTEMS 27
4.1 STANDARD SILICON PHOTODIODES 27
4.2 VIDICON TV CAMERAS 28
4.3 CHARGE-COUPLED DEVICES (CCD) 28
5 CONCLUSIONS 29
4. KINETIC STUDIES ON FLUORESCENCE PROBES USING SYNCHROTRON RADIATION
BY WOLFGANG RETTIG 31
1 INTRODUCTION: ADIABATIC PHOTOCHEMICAL PRODUCT FORMATION AND
FLUORESCENCE PROBES 31
2 FLUORESCENCE KINETICS AND ITS APPLICATION TO TICT STATES . . 33
2.1 THE PRINCIPLE OF TICT STATES 33
2.2 THE TIME STRUCTURE OF SYNCHROTRON RADIATION 34
3 HOW TO CONSTRUCT TICT FLUORESCENCE PROBES 35
3.1 PRINCIPAL APPROACHES 35
4 TICT PROBES IN BIOLOGY 42
5 APPLICATION OF TICT AND OTHER RELAXING PROBES IN LIQUID AND
RIGID MEDIA 43
6 PROTON TRANSFER FLUORESCENCE PROBES 45
7 REFERENCES 46
5. DYNAMICS AND GEOMETRY IN DIMERIC FLAVOPROTEINS FROM FLUORESCENCE
RELAXATION SPECTROSCOPY
BY PHILIPPE I. H. BASTIAENS AND ANTONIE J. W. G. VISSER 49
1 INTRODUCTION 49
2 LIPOAMIDE DEHYDROGENASE AND GLUTATHIONE REDUCTASE AS
BIOPHYSICAL SYSTEMS 51
3 FLUORESCENCE METHODOLOGY 52
3.1 MOLECULAR RELAXATION SPECTROSCOPY 52
3.2 FLUORESCENCE LIFETIME DISTRIBUTIONS 54
4 EXAMPLES 58
5 CONCLUSIONS 62
6 REFERENCES 62
6. FLUORESCENCE SPECTROSCOPY OF LIGHT SCATTERING MATERIALS
BY DIETER OELKRUG, ULRIKE MAMMEL, MANFRED BRUN, REINER GIINTHER
AND STEFAN UHL 65
1 INTRODUCTION 65
2 SPATIAL INTENSITY DISTRIBUTION OF FLUORESCENCE AND DETERMINATION
OF FLUORESCENCE YIELDS 65
CONTENTS XI
3 SENSITIVITY AND CONCENTRATION DEPENDENCE OF FLUORIMETRIC
ANALYSIS 67
4 CHEMICAL INTERACTIONS BETWEEN FLUOROPHORE AND SUBSTRATE . . 69
5 PROBLEMS OF QUANTITATIVE FLUORESCENCE SPECTROSCOPY .
.
.
. 72
5.1 REABSORPTION AND REEMISSION 72
5.2 INHOMOGENEOUS DISTRIBUTION OF THE FLUOROPHORES .
.
.
. 74
5.3 REFLECTANCE SPECTROSCOPY OF FLUORESCENT SAMPLES .
.
.
. 77
6
REFERENCES 78
7. OPTICAL SENSORS BASED ON FLUORESCENCE QUENCHING
BY WOLFGANG TRETTNAK 79
1 INTRODUCTION 79
2 SOME PRINCIPLES OF FLUORESCENCE QUENCHING 79
3 OXYGEN SENSORS 81
4 HALOTHANE SENSOR 83
5 SULPHUR DIOXIDE SENSOR 84
6 HALIDE SENSORS 84
7 HUMIDITY SENSOR 84
8 OXYGEN SENSORS AS TRANSDUCERS 85
8.1 HYDROGEN PEROXIDE SENSORS 85
8.2 GLUCOSE SENSORS 86
8.3 OTHER BIOSENSORS 86
9 APPLICATIONS 87
10 FUTURE ASPECTS 88
11 REFERENCES 89
PART 2. NEW APPLICATIONS OF FLUOROMETRY
8. FLUORESCENCE IN FOREST DECLINE STUDIES
BY H. SCHNECKENBURGER AND W. SCHMIDT 93
1 INTRODUCTION 93
2 MATERIALS AND METHODS 95
2.1 PLANT MATERIALS 95
2.2 MEASURING DEVICES 96
3 RESULTS AND DISCUSSION 98
3.1 CHLOROPHYLL CONTENT 98
3.2 FLUORESCENCE SPECTRA 98
3.3 PICOSECOND KINETICS 99
3.4 LONG TERM DELAYED LUMINESCENCE (LDL) 103
3.5 INDUCTION KINETICS OF DELAYED LUMINESCENCE 106
4 RESUME 108
5 REFERENCES 108
9. FLUORESCENCE MICROSCOPY STUDIES OF STRUCTURE FORMATION IN
SURFACTANT MONOLAYERS
BY HANS RIEGLER AND HELMUTH MOHWALD I
L
L
XII CONTENTS
1 INTRODUCTION I
L
L
2 PRINCIPLES OF THE MICROFLUORESCENCE TECHNIQUE 112
3 INVESTIGATIONS ON THE PLANE WATER SURFACE 113
3.1 DOMAIN SUPERLATTICE 114
3.2 DOMAIN SHAPE AND SIZE 116
3.3 NUMBER OF DOMAINS 118
4 INVESTIGATIONS DURING THE HYDROPHILIC LANGMUIR-BLODGETT
TRANSFER 120
4.1 SUBSTRATE-MEDIATED CONDENSATION WITH A STATIONARY
SUBSTRATE 121
4.2 MORPHOLOGICAL INSTABILITIES OF THE LC GROWTH FRONT .
.
. 122
4.3
SUBSTRATE-MEDIATED
CONDENSATION AND LOCAL DYE
DISTRIBUTION DURING CONTINUOUS LB-TRANSFER 123
5 CONCLUSIONS 126
6 REFERENCES 127
10. FLUORESCENCE LIFETIME IMAGING AND APPLICATION TO CA
2+
IMAGING
BY JOSEPH R. LAKOWICZ, HENRYK SZMACINSKI, KASIMIERZ
NOWACZYK, KLAUS BEMDT AND MICHAEL L. JOHNSON 129
ABBREVIATIONS 129
1 INTRODUCTION 129
2 CREATION OF LIFETIME IMAGES 131
3 INSTRUMENTATION FOR FLIM 133
4 EXPERIMENTAL METHODS 134
5 RESULTS 134
5.1 FLIM OF LIFETIME STANDARDS 134
5.2 FLIM OF QUIN-2 138
5.3 PHASE SUPPRESSION IMAGING OF CA
2+
143
6 DISCUSSION 145
7 REFERENCES 146
11. ETHER PHOSPHOLIPIDS IN MEMBRANES: APPLICATIONS OF PHASE AND
STEADY-STATE FLUOROMETRY
BY ALBIN HERMETTER, ELMAR PRENNER, JOSEF LOIDL, EDWIN KALB,
ANDREAS SOMMER AND FRITZ PALTAUF 149
12. PYRENE-LABELLED LIPIDS AS FLUORESCENT PROBES IN STUDIES ON
BIOMEMBRANES AND MEMBRANE MODELS
BY PAAVO K.J. KINNUNEN, ANU KOIV AND PEKKA MUSTONEN .
.
.
. 159
1
INTRODUCTION 159
2 BASIC PHOTOPHYSICS OF PYRENE AND ITS LIPID DERIVATIVES .
.
. 159
3
STUDIES ON LIPID DYNAMICS 162
3.1 PHOSPHOLIPID PHASE SEPARATION 163
3.2 FORMATION OF LIPID SUPERLATTICES 164
3.3 ESTIMATION OF LIPOSOME EQUILIBRIUM LATERAL SURFACE
PRESSURE 165
CONTENTS XIII
4 PERIPHERAL LIGAND-MEMBRANE INTERACTIONS 166
4.1 FORMATION OF SUPERLATTICES OF MEMBRANE-BOUND
CYTOCHROME C 166
4.2 BINDING OF POLYSOMES TO ROUGH ENDOPLASMIC RETICULUM
MEMBRANE 167
5 FUTURE PERSPECTIVES 169
6 REFERENCES 169
13. OPTICAL DETECTION OF INTRACELLULAR ION CONCENTRATIONS
BY JAN SLAVIK 173
1 INTRODUCTION 173
2 THEORY OF COLOR CHANGE 176
3 CALIBRATION CURVE 180
4 HOW TO BRING THE INDICATOR DYE INTO THE CELL 180
5 HOW FLUORESCENCE IS MEASURED 181
6 LIMITATIONS 183
6.1 TEMPORAL RESOLUTION 183
6.2 SPATIAL RESOLUTION 183
6.3 TEMPORAL AND SPATIAL RESOLUTION 184
7 CONCLUSION 184
8 REFERENCES 184
PART 3. FLUORIMETRIC ANALYSIS
14. ANALYTICAL APPLICATIONS O
F
VERY NEAR-IR FLUORIMETRY
BY JAMES N. MILLER, MARK B. BROWN, NICHOLA J. SEARE AND
STEPHEN SUMMERFIELD 189
1 INTRODUCTION 189
2 VNIR FLUOROPHORES 190
2.1 XANTHENE DYES 190
2.2 PHENOXAZINE DYES 191
2.3 CARBOCYANINE AND MEROCYANINE DYES 191
3 INSTRUMENTATION 192
4 RESULTS AND APPLICATIONS 193
5 CONCLUSIONS 195
6 REFERENCES 196
15. FLUORESCENCE DETECTION IN FLOW INJECTION ANALYSIS
BY MIGUEL VALC&CEL AND M. DOLORES LUQUE DE CASTRO 197
1 INTRODUCTION 197
2 REACTIONS INVOLVED 198
3 TYPES OF MANIFOLD 201
4 CONTINUOUS SEPARATION TECHNIQUES COUPLED TO FI SYSTEMS . . 207
5 (BIO)CHEMICAL FLUORIMETRIC SENSORS IN FI SYSTEMS 208
6 FINAL REMARKS 210
7 REFERENCES 211
XIV CONTENTS
16. FLUORESCENCE SPECTROSCOPY IN ENVIRONMENTAL AND HYDROLOGICAL SCIENCES
BY MARVIN C. GOLDBERG AND EUGENE R. WEINER 213
1 INTRODUCTION 213
2 EEM STUDIES, MULTI-COMPONENT ANALYSIS, SPECTRAL SIGNATURES,
AND "FINGERPRINTING" 214
'3 NON-EEM FLUORESCENCE METHODS 21S
4 DETERMINATION OF HUMIC DISSOCIATION CONSTANTS 218
5 PHYSICAL CHARACTERISTICS AND FLUOROPHORE COMPOSITION OF FULVIC
AND HUMIC ACIDS 218
6 HYDROLOGIC TRANSPORT OF PHENOL AND P-CRESOL IN THE
SOUTH.PLATTE RIVER COLORADO 219
7 GAS CHROMATOGRAPHIC MEASUREMENTS 219
8 FLUORESCENCE MEASUREMENTS AND RESULTS . . 221
9 PATTERN RECOGNITION 222
10 SEMI-QUANTITATIVE METHOD FOR CALCULATON OF KNOWN MATERIALS
IN EEM SPECTRA 225
11 EEM SPECTRA OF HUMIC FRACTIONS 232
12 GROUNDWATER POLLUTION MECHANISMS 232
13 CONCLUSIONS 237
14 REFERENCES 240
PART 4. FLUORESCENCE IMMUNOASSAY
17. FLUORESCENCE POLARISATION IMMUNOASSAY
BY CHRISTIAN KLEIN, HANS-GEORG BATZ, BRIGITTE DRAEGER,
HANS-JOACHIM GUDER, RUPERT HERRMANN, HANS-PETER JOSEL,
ULRICH NAGELE, ROLAND SCHENK AND BERNDT VOGT 245
1 INTRODUCTION 245
2 FLUORESCENCE POLARISATION 245
3 FLUOROPHORES 247
4 DESIGN AND SCREENING OF TRACERS 251
5 SUMMARY 258
6 REFERENCES 258
18. PROGRESS IN DELAYED FLUORESCENCE IMMUNOASSAY
BY ILKKA HEMMILA 259
ABBREVIATIONS 259
1 TIME-RESOLVED FLUOROMETRY 259
2 LONG-DECAY-TIME LUMINESCENT PROBES 259
2.1 CHELATE LABELS 260
3 TIME-RESOLVED FLUOROIMMUNOASSAYS 260
3.1 FLUORESCENCE ENHANCEMENT BASED ASSAYS 261
3.2 CHELATE SATURATION TECHNIQUES 262
3.3 ASSAYS WITH STABLE FLUORESCENT CHELATE LABELS 262
4 DOUBLE-, TRIPLE- AND QUADRUPLE-LABEL ASSAYS 263
5 HOMOGENEOUS ASSAYS 264
CONTENTS XV
6 DNA HYBRIDIZATION ASSAYS 264
7 FUTURE PROSPECTS 265
8 REFERENCES 265
19. CHEMILUMINESCENCE DETECTION IN IMMUNOCHEMICAL TECHNIQUES.
APPLICATIONS TO ENVIRONMENTAL MONITORING
BY EUGENE H. J. M. JANSEN 267
1 INTRODUCTION 267
2 CHEMILUMINESCENT LABELS IN IMMUNOASSAYS 267
2.1 TRANSIENT LABELS 268
2.2 ENZYME LABELS 269
2.3 ADVANTAGES OF ENZYME LABELS 271
2.4 DISADVANTAGES OF ENZYME LABELS 272
2.5 COMPARISON OF ENZYME LABELS 272
3 DETECTION OF CHEMILUMINESCENCE 273
3.1 LUMINOMETERS 273
3.2 OTHER DETECTION DEVICES 274
4 APPLICATIONS TO ENVIRONMENTAL MONITORING 274
4.1 IMMUNOASSAYS 274
4.2 BIOTRANSFORMATION ENZYMES 274
4.3 LUMINOUS BACTERIA 276
4.4 HYGIENE MONITORING 276
5 CONCLUSIONS 277
6 REFERENCES 278
PART 5. FLUORESCENCE IN BIOMEDICAL SCIENCES
20. FLUORESCENCE TRANSIENTS IN NEUROBIOLOGY: APPLICATIONS OF VOLTAGE
SENSITIVE AND ION INDICATOR DYES
BY WILLIAM N. ROSS 281
1 INTRODUCTION 281
2 VOLTAGE SENSITIVE DYES 282
3 CALCIUM INDICATOR DYES 283
4 SODIUM INDICATOR DYES 286
5 ION DIFFUSION 286
6 IMAGING TECHNOLOGY 288
7 FUTURE DIRECTIONS 288
8 REFERENCES 289
21. OPTICAL MONITORING OF POSTSYNAPTIC POTENTIAL IN THE EARLY EMBRYONIC
AVIAN BRAIN STEM USING A VOLTAGE-SENSITIVE DYE
BY K. KAMINO, T. SAKAI, Y. MOMOSE-SATO, H. KOMURO, A. HIROTA
AND K. SATO 291
1 INTRODUCTION 291
2 OPTICAL METHODS FOR MONITORING CELLULAR ELECTRICAL ACTIVITY 291
2.1 VOLTAGE-SENSITIVE DYES 291
XVI PREFACE
2.2 OPTICAL RECORDING SYSTEM 292
3 EMBRYONIC CHICK BRAIN STEM PREPARATIONS 292
4 OPTICAL DETECTION OF EVOKED ACTION POTENTIALS 295
5 POSTSYNAPTIC POTENTIAL SIGNALS 295
5.1 SYNAPTIC FATIGUE 298
5.2 EFFECTS OF EXTERNAL CA
2+
298
6 EVIDENCE FOR GLUTAMINERGIC EPSPS 299
7 DISCUSSION 299
8 REFERENCES 302
SUBJECT INDEX 305 |
any_adam_object | 1 |
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spelling | Fluorescence spectroscopy new methods and applications ed. by Otto S. Wolfbeis Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo ; Hong Kong ; Barcelona ; Budapest Springer 1993 txt rdacontent n rdamedia nc rdacarrier Fluoreszenzspektroskopie (DE-588)4017701-4 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 1991 Graz gnd-content Fluoreszenzspektroskopie (DE-588)4017701-4 s DE-604 Wolfbeis, Otto S. 1947-2023 (DE-588)1061164683 edt DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019107807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Fluorescence spectroscopy new methods and applications Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
subject_GND | (DE-588)4017701-4 (DE-588)1071861417 |
title | Fluorescence spectroscopy new methods and applications |
title_auth | Fluorescence spectroscopy new methods and applications |
title_exact_search | Fluorescence spectroscopy new methods and applications |
title_full | Fluorescence spectroscopy new methods and applications ed. by Otto S. Wolfbeis |
title_fullStr | Fluorescence spectroscopy new methods and applications ed. by Otto S. Wolfbeis |
title_full_unstemmed | Fluorescence spectroscopy new methods and applications ed. by Otto S. Wolfbeis |
title_short | Fluorescence spectroscopy |
title_sort | fluorescence spectroscopy new methods and applications |
title_sub | new methods and applications |
topic | Fluoreszenzspektroskopie (DE-588)4017701-4 gnd |
topic_facet | Fluoreszenzspektroskopie Konferenzschrift 1991 Graz |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019107807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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